Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,940,854 G→T 13.8% intergenic (+32/‑2) metZ → / → metW tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,940,8540GT13.8% 441.5 / 37.4 159intergenic (+32/‑2)metZ/metWtRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (47/89);  new base T (9/14);  total (56/103)
Fisher's exact test for biased strand distribution p-value = 8.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

TTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCA  >  CP009273/2940705‑2941001
                                                                                                                                                      |                                                                                                                                                   
ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTg                                                                                                                                                     <  2:2914289/150‑1 (MQ=255)
    aTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcgc                                                                                                                                                 <  1:142432/150‑1 (MQ=255)
     tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcgcg                                                                                                                                                <  2:2208954/150‑1 (MQ=255)
     tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcgcg                                                                                                                                                <  2:2732850/150‑1 (MQ=255)
     tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcgcg                                                                                                                                                <  1:3820637/150‑1 (MQ=255)
     tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCACCTCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAcgcg                                                                                                                                                <  2:2118510/150‑1 (MQ=255)
      gAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATT‑AAATTTGATGAAGTAAAAGCAGTACGGTg                                                                                                                                                     >  1:591127/1‑144 (MQ=255)
       aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTTTGTTGAAGT‑AAAGCAGTACGGTGAccgctc                                                                                                                                              >  2:55403/1‑145 (MQ=255)
       aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCggg                                                                                                                                              >  2:1724594/1‑150 (MQ=255)
       aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCggg                                                                                                                                              <  1:5017055/150‑1 (MQ=255)
       aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGAc                                                                                                                                                   <  1:1724600/145‑1 (MQ=255)
         aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGt                                                                                                                                            <  2:507170/150‑1 (MQ=255)
          aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTg                                                                                                                                           <  2:2947017/150‑1 (MQ=255)
          aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTg                                                                                                                                           <  2:776470/150‑1 (MQ=255)
          aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTg                                                                                                                                           <  2:776514/150‑1 (MQ=255)
             cgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGag                                                                                                                                        <  1:1620349/150‑1 (MQ=255)
                    aGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTg                                                                                                                                 <  2:1687557/150‑1 (MQ=255)
                     gTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTgg                                                                                                                                <  2:3136373/150‑1 (MQ=255)
                      tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGt                                                                                                                               <  2:376606/150‑1 (MQ=255)
                      tagaGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGt                                                                                                                               <  1:1575916/147‑1 (MQ=255)
                       cgaGTGCGCAGCCCCGGACCCGCGGAGCAGCCGCCGCGGAGCTCGCCGCGCCCCTAACCCGAAGGTCGGCGGTTCAACTCCGGCCCCCGCACCCAATTACAATTTGATGAAGG‑AAAGCCGTACGGTGACGCGGGGTGGAGCAGCCTggga                                                                                                                              <  2:4399318/148‑3 (MQ=9)
                       ataGTGCTCATCCACTGAGGCGGTGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGCAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa                                                                                                                              <  2:4399306/150‑1 (MQ=17)
                       ataGTGCGCATCCACGGTCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGCAGT‑AAAGCAGTACGGTGACGCGGGGTGTAGCAGCCTGGTa                                                                                                                              <  2:2395053/150‑1 (MQ=255)
                       ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa                                                                                                                              <  2:2394890/150‑1 (MQ=255)
                           tGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCtc                                                                                                                          <  2:3291517/150‑1 (MQ=255)
                            gcgcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcg                                                                                                                         <  1:2794779/150‑1 (MQ=255)
                               cATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTCCGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                                                      <  1:3166803/150‑1 (MQ=255)
                                 tCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGTTCGTCggg                                                                                                                    <  1:4123690/150‑1 (MQ=255)
                                 tCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                                                    <  1:2481769/150‑1 (MQ=255)
                                       gACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATa                                                                                                              <  1:1980551/150‑1 (MQ=40)
                                       gACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATa                                                                                                              <  1:4614264/150‑1 (MQ=40)
                                          gcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc                                                                                                           >  1:1544537/1‑150 (MQ=40)
                                             gggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGa                                                                                                        <  2:187442/150‑1 (MQ=40)
                                                tGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAgg                                                                                                     <  2:3683797/150‑1 (MQ=40)
                                                  gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                                                                                   <  2:3524926/150‑1 (MQ=40)
                                                   agcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                                                  <  2:996662/150‑1 (MQ=255)
                                                   agcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                                                  <  2:4134352/150‑1 (MQ=255)
                                                      tgcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCNTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGtcgtcg                                                                                               >  1:2676008/2‑150 (MQ=255)
                                                      agcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTGCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGtcgtcg                                                                                               <  2:4530735/150‑1 (MQ=255)
                                                       gcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCgg                                                                                              <  2:3274498/150‑1 (MQ=40)
                                                          tGGTAGCTCTTCTGGCTATTAACCCGAAGGTCGTCGGTTCAAATCCTGCCCGCGCACACAATTAAAATTTGATGTAGA‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTc                                                                                           <  1:40025/150‑1 (MQ=11)
                                                           ggTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCa                                                                                          <  2:1945741/150‑1 (MQ=40)
                                                           ggTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCa                                                                                          <  2:1569985/150‑1 (MQ=40)
                                                            gTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAACTAGGCGGAAGG‑AGGTCTGTAAGGTTACGAGCGGTGGAGCTTCCTGGTAGCTCGGCGGGCGCATAACCCGACGGGCTTCGGTACaa                                                                                         >  2:4740411/1‑150 (MQ=2)
                                                               gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                                                      <  1:4129902/150‑1 (MQ=255)
                                                                cTCGTCTGTCTCATAACCCGAAGGTCGTCGGTTCAAATCCGTCACCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCATTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATcc                                                                                     <  1:778725/150‑1 (MQ=14)
                                                                cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATcc                                                                                     >  1:449436/1‑150 (MQ=40)
                                                                  cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                                                   <  2:1405209/150‑1 (MQ=40)
                                                                     cgGGCTCTAATCCCTAAGGTCTTCGGTTCAAATCCTGACCCAGCTAACAATTATAATTTGCTGTAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGccc                                                                                <  2:4695111/150‑1 (MQ=11)
                                                                      gggCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGcccc                                                                               >  2:4878921/1‑150 (MQ=40)
                                                                       ggCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGccccc                                                                              <  1:2707603/150‑1 (MQ=40)
                                                                        gCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCg                                                                             <  1:1077823/150‑1 (MQ=255)
                                                                        gCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCg                                                                             <  1:4662218/150‑1 (MQ=40)
                                                                        gCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCg                                                                             <  2:310977/150‑1 (MQ=40)
                                                                          tCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCa                                                                           <  1:797056/150‑1 (MQ=40)
                                                                            aTAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                                                         >  1:3656868/1‑150 (MQ=40)
                                                                             tAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCCAcc                                                                        >  1:1342538/1‑150 (MQ=255)
                                                                              aaCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTCCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                       <  1:79907/150‑1 (MQ=255)
                                                                              aaCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                       >  1:1514582/1‑150 (MQ=40)
                                                                                   gAAGGTCGTCGGTTCTAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AATGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                  <  2:3514653/150‑1 (MQ=255)
                                                                                   gAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                  <  1:4937150/150‑1 (MQ=255)
                                                                                   gAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                  <  2:1016770/150‑1 (MQ=255)
                                                                                     aGGTCGTCGGTTCAAATCCGGCCCGCGCAACCAATTATATTTTGTTGTAGT‑ATTGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                                <  2:1772640/150‑1 (MQ=14)
                                                                                      ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAtt                                                               <  1:1305667/150‑1 (MQ=255)
                                                                                           tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAGGACGT‑AAAGCAGTACGGTGACGCTGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCCCAACCAAACAAAGTtgatg                                                          >  1:2851916/1‑150 (MQ=14)
                                                                                             tgTTCAAATCCGTCCCCCTCAAACAATTAAATTTTGATGAAGT‑ATCGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGaa                                                        <  2:4494969/149‑1 (MQ=17)
                                                                                             ggTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGACGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAACTTTGAGGaa                                                        >  1:920966/1‑150 (MQ=255)
                                                                                              gTTCAAATCCGGCCCCCGCAACCAATTATACTTTGATGAAGT‑ATAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAg                                                       <  2:1375192/150‑1 (MQ=255)
                                                                                              gTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAg                                                       >  1:3113936/1‑150 (MQ=255)
                                                                                              gTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAg                                                       >  1:1427106/1‑150 (MQ=255)
                                                                                              gTTCAAATCCGGCCCCCGCAACCAATTAAAATTATATGAAGT‑AAAGCAGTACGGTGACGCGGGCTGGAGCAGCCTGGTAGCTCGTCGGGCTAATAACCCGAAGGTCGTCGGTTCAAATCCGGCACCCGCAACCAATAAAATATgaagaaa                                                       >  1:461834/1‑149 (MQ=14)
                                                                                               ttCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGGACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGTAGGTCGTCGGTTCCAATCCGGCCCCCGCAACCAATGAATTTtgctccaga                                                      >  1:2336863/1‑143 (MQ=14)
                                                                                                tCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTa                                                     >  2:1433313/1‑150 (MQ=255)
                                                                                                  aaaTCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTGGATCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATACGGCCCCCGCAACCAATCAATTGTGATCAAGTaat                                                   >  1:2112272/1‑149 (MQ=14)
                                                                                                  aaaTCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTaaa                                                   <  1:2016638/150‑1 (MQ=255)
                                                                                                  aaaTCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGCAGTaaa                                                   >  1:3466246/1‑150 (MQ=255)
                                                                                                     tCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGc                                                <  1:4999544/150‑1 (MQ=255)
                                                                                                       cGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAg                                              <  2:1350538/150‑1 (MQ=255)
                                                                                                         gCCCCCGCAACCAATTAAAATTTGATGAATT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTa                                            <  2:4035046/150‑1 (MQ=255)
                                                                                                            cccGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTCAAATTTGATGAAGTAAAAGCAGTACgg                                         >  1:4044656/1‑150 (MQ=255)
                                                                                                              cgaaaCCAATTATATTTTGTTGTAGT‑AAAGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTg                                       <  1:4263566/147‑1 (MQ=18)
                                                                                                                   ccAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAGTTGATGAAGTAAAAGCAGTACGGTGAcgcg                                  >  2:522234/1‑150 (MQ=255)
                                                                                                                      aTTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCgggg                               <  1:1233654/150‑1 (MQ=255)
                                                                                                                       ttACAATTTGATGAAGT‑AACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGt                              <  2:1545735/150‑1 (MQ=255)
                                                                                                                          aaaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGTAGTAAAAGCAGTACGGTGACGCGGGGTGGa                           >  2:5157259/1‑150 (MQ=255)
                                                                                                                           aaTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAGTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGag                          >  1:2251020/1‑150 (MQ=255)
                                                                                                                             tttGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGagca                        <  1:4344304/150‑1 (MQ=255)
                                                                                                                               tgatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATATGATGAAGTAAAAGCAGTACTGTGACGCGGGCTGGagcagc                      >  2:4791408/1‑150 (MQ=255)
                                                                                                                                gatgaAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                     >  1:4864561/1‑150 (MQ=255)
                                                                                                                                        tctccgaGTCCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGc              <  1:1132312/144‑1 (MQ=255)
                                                                                                                                         aaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt             >  2:3068224/1‑150 (MQ=255)
                                                                                                                                          acgCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtc            <  1:117989/148‑1 (MQ=255)
                                                                                                                                          aaGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtc            >  1:3206922/1‑150 (MQ=255)
                                                                                                                                              aGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg        <  2:1544537/150‑1 (MQ=255)
                                                                                                                                              aGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAGTTGATGAAGTAAATGCAGTACGGTGAAGAGGGGTGGAGAAGACTGGAAGCtcgtcg        >  2:2131206/1‑150 (MQ=14)
                                                                                                                                                 aCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGc     >  1:2354935/1‑150 (MQ=255)
                                                                                                                                                  cGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCt    >  1:2381320/1‑150 (MQ=255)
                                                                                                                                                  cGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGAAGCCTGGTCGCTCGTCGGGCt    >  2:1401559/1‑150 (MQ=255)
                                                                                                                                                   ggTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAATGTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTAGCTCGTCGGGCTc   >  1:3417052/1‑150 (MQ=255)
                                                                                                                                                   ggTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTc   <  2:5009340/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCA  >  CP009273/2940705‑2941001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑