Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,941,341 G→A 100% L343L (CTC→CTT)  amiC ← N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,941,3410GA99.0% 992.0 / ‑0.9 298L343L (CTC→CTT) amiCN‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (152/143);  total (152/146)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 298 total aligned reads displayed.

CTGGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCAG  >  CP009273/2941194‑2941487
                                                                                                                                                   |                                                                                                                                                  
ctgGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTGACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAg                                                                                                                                                  <  2:1137687/150‑1 (MQ=255)
 tgGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGc                                                                                                                                                 >  2:1787666/1‑150 (MQ=255)
 tgGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGc                                                                                                                                                 >  2:3472944/1‑150 (MQ=255)
  ggAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAAACCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCt                                                                                                                                                >  1:52962/1‑150 (MQ=255)
    aaaGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTa                                                                                                                                              <  2:138503/150‑1 (MQ=255)
    aaaGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTa                                                                                                                                              <  1:1699588/150‑1 (MQ=255)
     aaGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAt                                                                                                                                             >  2:662788/1‑150 (MQ=255)
     aaGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAt                                                                                                                                             <  2:1545323/150‑1 (MQ=255)
     aaGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAt                                                                                                                                             <  1:209742/150‑1 (MQ=255)
     aaGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAt                                                                                                                                             >  2:4194927/1‑150 (MQ=255)
      aGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAtt                                                                                                                                            <  1:3966733/150‑1 (MQ=255)
        tCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCa                                                                                                                                          >  1:3757214/1‑150 (MQ=255)
         cGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAg                                                                                                                                         <  2:2837450/150‑1 (MQ=255)
          gCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGt                                                                                                                                        >  2:3213135/1‑150 (MQ=255)
          gCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGt                                                                                                                                        >  2:564468/1‑150 (MQ=255)
          gCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGt                                                                                                                                        <  2:1915638/150‑1 (MQ=255)
                 tCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCtt                                                                                                                                 <  2:4670418/150‑1 (MQ=255)
                 tCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCATGTTGATTTTACCAAGCATATTCAGTAACGCtt                                                                                                                                 >  2:2625576/1‑150 (MQ=255)
                 tCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTATAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAATCTTATTCAGTACCTCtt                                                                                                                                 >  1:4411237/1‑150 (MQ=255)
                  cAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGACTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCttt                                                                                                                                >  2:4809699/1‑150 (MQ=255)
                          gCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACt                                                                                                                        <  2:3270789/150‑1 (MQ=255)
                          gCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACt                                                                                                                        >  1:4362301/1‑150 (MQ=255)
                          actctACCTCAACTTTACTGATAAAAGCCGTTTCGACCAGAATGGAGGGAATATCTGTTGCATTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTATATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTATACCAAACt                                                                                                                        >  2:2957733/2‑150 (MQ=255)
                           ctctTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACtt                                                                                                                       <  2:4448536/150‑1 (MQ=255)
                            tctTCCACAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTc                                                                                                                      >  2:1519793/1‑150 (MQ=255)
                             tttCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCa                                                                                                                     >  1:696815/2‑150 (MQ=255)
                             ctTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCa                                                                                                                     <  1:1562803/150‑1 (MQ=255)
                                 cTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCt                                                                                                                 <  2:1633342/150‑1 (MQ=255)
                                 cTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCt                                                                                                                 >  1:329339/1‑150 (MQ=255)
                                 cTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCt                                                                                                                 <  1:359392/150‑1 (MQ=255)
                                 cTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCt                                                                                                                 <  2:5005999/150‑1 (MQ=255)
                                     aCGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCg                                                                                                             >  1:1107400/1‑150 (MQ=255)
                                     aCGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCg                                                                                                             <  1:2812831/150‑1 (MQ=255)
                                        ttACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCa                                                                                                          <  1:1273857/150‑1 (MQ=255)
                                           cTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCACTg                                                                                                       >  1:2666716/1‑150 (MQ=255)
                                             gATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGt                                                                                                     <  2:2259424/150‑1 (MQ=255)
                                             gATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGt                                                                                                     >  1:2894000/1‑150 (MQ=255)
                                               tAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCa                                                                                                   <  1:4519796/150‑1 (MQ=255)
                                               tAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCa                                                                                                   <  2:280302/150‑1 (MQ=255)
                                               tAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCa                                                                                                   <  2:3099993/150‑1 (MQ=255)
                                               tAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCa                                                                                                   >  1:1945686/1‑150 (MQ=255)
                                                aaaCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAg                                                                                                  <  1:806252/150‑1 (MQ=255)
                                                aaaCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAg                                                                                                  <  2:4121970/150‑1 (MQ=255)
                                                  aCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCg                                                                                                <  2:2155348/150‑1 (MQ=255)
                                                  aCGCCGTGTCGACCAGACTGGAGGGAATCTCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCg                                                                                                <  2:4364291/150‑1 (MQ=255)
                                                    gCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGAc                                                                                              <  1:92617/150‑1 (MQ=255)
                                                     ccGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACt                                                                                             >  2:2176576/1‑150 (MQ=255)
                                                     ccGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTGAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACt                                                                                             <  2:3072963/150‑1 (MQ=255)
                                                       gTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGt                                                                                           >  2:3532561/1‑150 (MQ=255)
                                                        tttCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTa                                                                                          <  1:2976567/150‑1 (MQ=255)
                                                         ttCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTAc                                                                                         >  2:1229163/1‑150 (MQ=255)
                                                          tCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTAcc                                                                                        >  2:4483498/1‑150 (MQ=255)
                                                           cGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCa                                                                                       >  2:4344369/1‑150 (MQ=255)
                                                               cAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATc                                                                                   >  2:1457644/1‑150 (MQ=255)
                                                               cAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATc                                                                                   >  2:379352/1‑150 (MQ=255)
                                                                aGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCCGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCg                                                                                  <  1:4787909/150‑1 (MQ=255)
                                                                   aTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAAc                                                                               >  2:4607660/1‑150 (MQ=255)
                                                                      gAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGTTTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCCGTACCGCTTTACCAAACTTCAGGCTGTCGGCACTGGTCAGCGACTGTACCATATCGAACATg                                                                            <  1:1065055/150‑1 (MQ=255)
                                                                         ggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgtg                                                                         <  2:1019103/150‑1 (MQ=255)
                                                                          gAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgtgt                                                                        >  1:1179511/1‑150 (MQ=255)
                                                                              atCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATTGTCAGCGACTGTACCATATCGAAAATGGTGTGGTc                                                                    >  2:5098018/1‑150 (MQ=255)
                                                                              atCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTc                                                                    >  2:890879/1‑150 (MQ=255)
                                                                              atCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTc                                                                    <  1:5095200/150‑1 (MQ=255)
                                                                              atCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTc                                                                    >  2:2004015/1‑150 (MQ=255)
                                                                                 tGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGAc                                                                 <  2:1727202/150‑1 (MQ=255)
                                                                                     gCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAg                                                             >  1:1237867/1‑150 (MQ=255)
                                                                                        tttAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCgg                                                          >  1:626786/1‑150 (MQ=255)
                                                                                          tAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTc                                                        >  1:274649/1‑150 (MQ=255)
                                                                                          tAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTc                                                        >  1:3528395/1‑150 (MQ=255)
                                                                                             tACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCAcc                                                     <  2:523852/150‑1 (MQ=255)
                                                                                             tACGGCAAACACGGCCTGTTCAACTTTATTTTTATGCAGCTTGTTTATTTTACCACGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCAcc                                                     <  2:2276679/150‑1 (MQ=255)
                                                                                                ggCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCt                                                  >  1:3372489/1‑150 (MQ=255)
                                                                                                 gCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCtt                                                 <  2:2386717/150‑1 (MQ=255)
                                                                                                  cAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACAGCTTTACCAAACTTCAGGCTGTCGGCAAAGGTCAGCGACTGAACCATATCGAACATGGATTGGTCGACATATCGGTCACCGCttt                                                >  1:3059411/1‑150 (MQ=255)
                                                                                                      cccGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCt                                            >  1:2481108/1‑150 (MQ=255)
                                                                                                       ccGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTc                                           >  2:2212571/1‑150 (MQ=255)
                                                                                                       ccGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTc                                           <  2:4544482/150‑1 (MQ=255)
                                                                                                          gCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACg                                        <  1:4760779/150‑1 (MQ=255)
                                                                                                            cTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGcc                                      >  1:4463808/1‑150 (MQ=255)
                                                                                                             tGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGcca                                     <  1:2328067/150‑1 (MQ=255)
                                                                                                             tGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGcca                                     <  2:2894000/150‑1 (MQ=255)
                                                                                                             tGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGcca                                     <  2:3682266/150‑1 (MQ=255)
                                                                                                               ttCAACTTGATTTTTATGCTGATTGTTGATTTTACCAAGCTTATTCAGTACCGATTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGccacc                                   <  1:5130581/150‑1 (MQ=255)
                                                                                                               ttCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGccacc                                   >  1:4181350/1‑150 (MQ=255)
                                                                                                                 cAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCaa                                 >  1:4222323/1‑150 (MQ=255)
                                                                                                                      tGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAg                            <  1:645695/150‑1 (MQ=255)
                                                                                                                        aTTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCACTGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTc                          <  2:7457/150‑1 (MQ=255)
                                                                                                                        aTTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTc                          >  2:253888/1‑150 (MQ=255)
                                                                                                                              aTGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGc                    >  1:4164287/1‑150 (MQ=255)
                                                                                                                               tGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTGCCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCg                   >  2:1659595/1‑150 (MQ=255)
                                                                                                                                gCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCACGTCCGAGGCGt                  >  2:3590280/1‑150 (MQ=255)
                                                                                                                                  aGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCATGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTc                >  1:2140883/1‑150 (MQ=255)
                                                                                                                                   gCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCt               <  1:2043829/150‑1 (MQ=255)
                                                                                                                                    cTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTg              >  1:961204/1‑150 (MQ=255)
                                                                                                                                     ttgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTgg             >  2:3019697/1‑150 (MQ=255)
                                                                                                                                     ttgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTgg             >  2:4627109/1‑150 (MQ=255)
                                                                                                                                      tgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTggg            <  1:1885797/150‑1 (MQ=255)
                                                                                                                                       gttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGt           <  2:2326939/150‑1 (MQ=255)
                                                                                                                                         tgATTTTACAAAGATTTTTCAGTACCGCTTTACCATACTTCTGGCTGTCGGAAATGTTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGttt         <  1:240971/150‑1 (MQ=255)
                                                                                                                                                aCCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGATGCGTTCTGGGTTtgttcaag  >  2:1694641/1‑145 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCAG  >  CP009273/2941194‑2941487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑