Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,959,004 G→A 100% I131I (ATC→ATT)  lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,959,0040GA99.2% 801.1 / ‑2.8 241I131I (ATC→ATT) lgtphosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (133/105);  total (135/106)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 241 total aligned reads displayed.

GTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGG  >  CP009273/2958857‑2959150
                                                                                                                                                   |                                                                                                                                                  
gttggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAAt                                                                                                                                                  >  2:1240440/1‑150 (MQ=255)
 ttggttTGCAGCAGCAAAATATCGTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATa                                                                                                                                                 <  2:4567658/150‑1 (MQ=255)
  tggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaa                                                                                                                                                >  1:2881107/1‑150 (MQ=255)
   ggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaaa                                                                                                                                               <  2:4954314/150‑1 (MQ=255)
   ggttTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATaaa                                                                                                                                               <  1:2401605/150‑1 (MQ=255)
       tGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCa                                                                                                                                           >  1:2454441/1‑150 (MQ=255)
        gcagcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAAGGAGTGGTGCAATAAAATCag                                                                                                                                          >  2:4234109/1‑150 (MQ=255)
         cagcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCaga                                                                                                                                         <  2:1931773/150‑1 (MQ=255)
          agcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCagag                                                                                                                                        <  1:3770678/150‑1 (MQ=255)
           gcagcaAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAgaga                                                                                                                                       <  2:3665147/150‑1 (MQ=255)
               caAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTg                                                                                                                                   <  1:4173418/150‑1 (MQ=255)
                   atatCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaag                                                                                                                               >  2:3166547/1‑150 (MQ=255)
                    tatCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaaga                                                                                                                              >  1:43439/1‑150 (MQ=255)
                    tatCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaaga                                                                                                                              <  1:1880729/150‑1 (MQ=255)
                     atCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGgaagaa                                                                                                                             <  1:4995377/150‑1 (MQ=255)
                       cttctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAgg                                                                                                                           >  1:4626989/1‑150 (MQ=255)
                          ctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCTATAAAATCAGAGACCTGGAAGAAGGAAc                                                                                                                        <  2:3945932/150‑1 (MQ=255)
                          ctGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAAc                                                                                                                        <  1:55137/150‑1 (MQ=255)
                           tGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACg                                                                                                                       <  2:1328218/150‑1 (MQ=255)
                             tACGGGAGCCAGGGAACAGCATGGGAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGtt                                                                                                                     <  2:1426349/150‑1 (MQ=255)
                             tACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGtt                                                                                                                     <  2:2820468/150‑1 (MQ=255)
                             tACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGtt                                                                                                                     <  1:3097406/150‑1 (MQ=255)
                             tACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGtt                                                                                                                     >  1:4050568/1‑150 (MQ=255)
                              aCGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGttt                                                                                                                    <  2:3050520/150‑1 (MQ=255)
                               cgtgAGCCATGGAACAGCATGGAAAACGGGAAGTTCGGGTCAACGCGGCCCCAGACTTCAACGTTTATAATGTGGCCCAGACTACCGGCACCAAGAACAAACGTTATGATTGGTGCAATAAAATAAGAGACATGGTAGAATGTACGTTTa                                                                                                                   <  2:3026504/147‑1 (MQ=255)
                               cGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTa                                                                                                                   <  2:2876158/150‑1 (MQ=255)
                                 ggAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGt                                                                                                                 >  2:815676/1‑150 (MQ=255)
                                    gCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcg                                                                                                              >  1:4789492/1‑150 (MQ=255)
                                       aGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGACTGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggcg                                                                                                           >  1:1986605/1‑150 (MQ=255)
                                       aGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggcg                                                                                                           >  1:4561578/1‑150 (MQ=255)
                                       aGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggcg                                                                                                           <  1:1349350/150‑1 (MQ=255)
                                       aGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggcg                                                                                                           >  1:2805276/1‑150 (MQ=255)
                                        gggAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggcgg                                                                                                          >  2:4463441/1‑150 (MQ=255)
                                        gggAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTAcggcgg                                                                                                          >  1:2736149/1‑150 (MQ=255)
                                         ggAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACggcggg                                                                                                         <  2:886354/150‑1 (MQ=255)
                                            aCAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGa                                                                                                      >  1:815192/1‑150 (MQ=255)
                                              aGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAg                                                                                                    <  1:3269407/150‑1 (MQ=255)
                                              aGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAg                                                                                                    <  1:4502322/150‑1 (MQ=255)
                                                 aTGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATa                                                                                                 >  2:1042453/1‑150 (MQ=255)
                                                 aTGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATa                                                                                                 <  1:4146105/150‑1 (MQ=255)
                                                 aTGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATa                                                                                                 >  1:1939604/1‑150 (MQ=255)
                                                   ggCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAat                                                                                               <  2:110120/150‑1 (MQ=255)
                                                         cGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATcac                                                                                         >  1:4576265/1‑150 (MQ=255)
                                                          gggAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATcacc                                                                                        >  1:2132797/1‑150 (MQ=255)
                                                           ggAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATcacca                                                                                       <  2:1292248/150‑1 (MQ=255)
                                                                ttCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATa                                                                                  <  2:1814479/150‑1 (MQ=255)
                                                                 tCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATaa                                                                                 >  1:1055425/1‑150 (MQ=255)
                                                                       cAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGACTGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCaa                                                                           >  1:2303876/1‑150 (MQ=255)
                                                                         aCGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATc                                                                         >  1:1448336/1‑150 (MQ=255)
                                                                          cgcgGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCCAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCa                                                                        <  1:929861/150‑1 (MQ=255)
                                                                          cgcgGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCa                                                                        <  2:3132264/150‑1 (MQ=255)
                                                                              gCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgcc                                                                    <  1:4480923/150‑1 (MQ=255)
                                                                              gCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgcc                                                                    <  1:4705972/150‑1 (MQ=255)
                                                                              gCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgcc                                                                    <  1:1623159/150‑1 (MQ=255)
                                                                               ccccACAATTCTCTGTTTATATAGTTGCCCAGACGCCCGGCACCAAGACCACACGGAATGAGTTGTGCAATAAAATCAGAGACCTGGTAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCTATCAGgccg                                                                   <  2:4774051/150‑1 (MQ=255)
                                                                                cccACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgccgc                                                                  >  1:4623089/1‑150 (MQ=255)
                                                                                 ccACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGgccgcc                                                                 <  1:3156028/150‑1 (MQ=255)
                                                                                    caATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTg                                                              <  2:3905957/150‑1 (MQ=255)
                                                                                      aTTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGa                                                            >  2:4926899/1‑150 (MQ=255)
                                                                                      aTTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGa                                                            <  2:1383216/150‑1 (MQ=255)
                                                                                      aTTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGa                                                            >  2:4926854/1‑150 (MQ=255)
                                                                                      aTTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGa                                                            >  1:1204979/1‑150 (MQ=255)
                                                                                       ttCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGaa                                                           >  2:1126357/1‑150 (MQ=255)
                                                                                        tCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGaaa                                                          <  2:1006765/150‑1 (MQ=255)
                                                                                          aCCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGa                                                        >  1:3008937/1‑150 (MQ=255)
                                                                                               taataaAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgc                                                   >  1:1151017/1‑150 (MQ=255)
                                                                                               taataaAGGTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgc                                                   <  2:3450484/150‑1 (MQ=255)
                                                                                                 ataaAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATgccg                                                 >  1:1665945/1‑150 (MQ=255)
                                                                                                    aaGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGccgccg                                              >  1:2856059/1‑150 (MQ=255)
                                                                                                    aaGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGccgccg                                              >  1:3415450/1‑150 (MQ=255)
                                                                                                     aGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGt                                             <  1:1536374/150‑1 (MQ=255)
                                                                                                       ttGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTcc                                           >  1:2924078/1‑150 (MQ=255)
                                                                                                       ttGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTcc                                           >  2:3783950/1‑150 (MQ=255)
                                                                                                         gCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCa                                         <  1:4292629/150‑1 (MQ=255)
                                                                                                          cccAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAg                                        <  2:1512135/150‑1 (MQ=255)
                                                                                                            cAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGac                                      >  1:3854519/1‑150 (MQ=255)
                                                                                                             aGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGaca                                     >  1:4465895/1‑150 (MQ=255)
                                                                                                              gACGCCCGGCACCAAGACCAAACGGAATTAGTGGTGAAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGacac                                    <  1:1019918/150‑1 (MQ=255)
                                                                                                              gACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGacac                                    <  2:4623089/150‑1 (MQ=255)
                                                                                                                 gCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACTGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGa                                 >  1:3998578/1‑150 (MQ=255)
                                                                                                                 gCCCGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGa                                 >  2:652368/1‑150 (MQ=255)
                                                                                                                   ccGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAAc                               <  1:254231/150‑1 (MQ=255)
                                                                                                                    cGGCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACa                              >  1:3607453/1‑150 (MQ=255)
                                                                                                                    aggCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACa                              >  2:2588382/2‑150 (MQ=255)
                                                                                                                      gCACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGa                            <  1:1787855/150‑1 (MQ=255)
                                                                                                                       cACCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATACTCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGAt                           <  2:1604200/150‑1 (MQ=255)
                                                                                                                        aCCAAGACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATa                          <  2:2241638/150‑1 (MQ=255)
                                                                                                                             gACCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCg                     >  1:5131320/1‑150 (MQ=255)
                                                                                                                              aCCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCgg                    >  2:265732/1‑150 (MQ=255)
                                                                                                                              aCCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCgg                    >  2:2071765/1‑150 (MQ=255)
                                                                                                                              aCCAAACGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCgg                    >  2:3714686/1‑150 (MQ=255)
                                                                                                                                 aaaCGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATc                 <  1:4094572/150‑1 (MQ=255)
                                                                                                                                   aCGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGTCATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCgg               >  2:3250717/1‑150 (MQ=255)
                                                                                                                                   aCGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCgg               <  1:125772/150‑1 (MQ=255)
                                                                                                                                    cGGAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGc              >  2:5144825/1‑150 (MQ=255)
                                                                                                                                     ggAATGAGTGGTGCAATAAAATCAGAGACCTTGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACAAGGAACAGATACAGCGGATCGGcc             <  2:2856059/150‑1 (MQ=255)
                                                                                                                                     ggAATGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGcc             <  1:2658635/150‑1 (MQ=255)
                                                                                                                                         tGAGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGACAGACATGCCGCCGACCCAGACACGGAACAGATACAGCGGAGCGGCCCTaa         >  2:5077803/1‑150 (MQ=255)
                                                                                                                                           aGTGGTGCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAAc       <  2:811690/150‑1 (MQ=255)
                                                                                                                                            gtggtgCAATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACt      <  2:3592904/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGG  >  CP009273/2958857‑2959150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑