Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,039,409 T→G 12.4% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,039,4090TG12.4% 394.3 / 14.0 219intergenic (‑149/+118)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (88/99);  new base G (23/9);  total (111/108)
Fisher's exact test for biased strand distribution p-value = 1.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

TTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAG  >  CP009273/3039260‑3039557
                                                                                                                                                     |                                                                                                                                                       
tttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTACCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGt                                                                                                                                                         <  2:251605/150‑1 (MQ=255)
tttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGt                                                                                                                                                         >  1:1175615/1‑150 (MQ=255)
 ttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGtt                                                                                                                                                        >  1:499688/1‑150 (MQ=255)
        tCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCa                                                                                                                                                 <  2:3513852/150‑1 (MQ=255)
          cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGAACGCTTTTCCCCCTCGCCCTTTCAGGGATAGGTCCGGGGTTAGGGTAAATATTCTCGCCAGTGCCGGCCTGTTCCCCTCAcc                                                                                                                                               >  1:4216787/1‑150 (MQ=255)
            agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACAccca                                                                                                                                             >  2:1820237/1‑149 (MQ=255)
             gaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTa                                                                                                                                            <  2:4078757/150‑1 (MQ=255)
               aaaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAc                                                                                                                                          <  1:1818564/150‑1 (MQ=255)
                aaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAcc                                                                                                                                         >  2:262027/1‑150 (MQ=255)
                aaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCCCCCAAAcc                                                                                                                                         >  1:3350890/1‑150 (MQ=255)
                 aattaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCCCCCTAAccc                                                                                                                                        >  2:2649647/1‑150 (MQ=255)
                  attaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCct                                                                                                                                       >  2:2051082/1‑150 (MQ=255)
                     aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCATTGTTGGCACGCTTTTCCCCCTCGCCCTGTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTCACCCgctc                                                                                                                                    >  2:2927779/1‑150 (MQ=255)
                     aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCactc                                                                                                                                    >  2:5060666/1‑150 (MQ=255)
                       ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTc‑cc                                                                                                                                 <  1:3516563/150‑1 (MQ=255)
                       ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAAACCTCTc‑cc                                                                                                                                 >  2:2437183/1‑150 (MQ=255)
                           ttGACGTATCTTTGAGATTGATAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGAACCCAAACCCTCTC‑CCCaaa                                                                                                                             >  1:2914066/1‑150 (MQ=255)
                            tGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAg                                                                                                                            <  1:4282337/150‑1 (MQ=255)
                            tGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAg                                                                                                                            <  2:2729064/150‑1 (MQ=255)
                            tGACGTATCTTTGAGATTGCTAACAAACTGGCTCAAGCGTCAAGGTTGGCACGCTTTTCCCCCTCGCCCGTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAg                                                                                                                            <  2:4808403/150‑1 (MQ=255)
                                gTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGc                                                                                                                        <  2:4175317/150‑1 (MQ=255)
                                  aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTAGCCCTTTCAGTGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGa                                                                                                                      <  1:2500079/150‑1 (MQ=255)
                                    cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAgg                                                                                                                    <  1:2308025/150‑1 (MQ=255)
                                    cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCACCCCCGAAACCACTC‑CCCAAAAGGGccccg                                                                                                                    >  1:4890411/1‑146 (MQ=255)
                                     tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCCAAGGGGCGAggg                                                                                                                   >  2:4603349/1‑150 (MQ=255)
                                      ttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAgggg                                                                                                                  >  2:5019520/1‑150 (MQ=255)
                                       tGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGa                                                                                                                 <  2:3153939/150‑1 (MQ=255)
                                        gagaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCTCACCCAAACCCACAC‑CCCCAACGGGCGAgggggc                                                                                                                >  1:4556431/1‑148 (MQ=255)
                                        gagaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCCAACCCACAC‑CCCCAAAGGGCGGGGGGAc                                                                                                                >  1:4909351/1‑150 (MQ=255)
                                         agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACt                                                                                                               <  1:322957/150‑1 (MQ=255)
                                          gaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCACAGGGGCGAGGGGACTg                                                                                                              >  2:3291000/1‑150 (MQ=255)
                                              gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCACAC‑CCCAAAGGGGCGAGGGGACTGT‑CTg                                                                                                         >  1:2579334/1‑150 (MQ=255)
                                               cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGa                                                                                                        <  1:1713921/150‑1 (MQ=255)
                                                tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCCCCCAAACCCACTA‑CCCAAAGGGGCGAGGGGCCTGT‑CTGAg                                                                                                       >  2:2271388/1‑150 (MQ=255)
                                                     aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCCCACCCCAAACCACAC‑CCCCAAAGGGGGAGGGGGCGGT‑CTGATCCCt‑t                                                                                                 >  1:1318229/1‑150 (MQ=255)
                                                     aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACt‑t                                                                                                 <  2:225966/150‑1 (MQ=255)
                                                     aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCGCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGcccgt                                                                                                  >  1:717189/1‑146 (MQ=255)
                                                     aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTC‑CCCAAAGGGGCGCGGGGACTTT‑CTGAGCACt‑t                                                                                                 >  1:806111/1‑150 (MQ=255)
                                                        tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTg                                                                                              <  1:4635974/150‑1 (MQ=255)
                                                        tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACAC‑CCCCAACGGGCGAGGGGACTGT‑CTGAGCACA‑TTTg                                                                                              >  1:1982049/1‑150 (MQ=255)
                                                        tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCACACCCAAAACCAAAC‑CCCCAAAGGGCGCGGGGGCTGT‑CTGAGCAcagttg                                                                                               >  1:1756445/1‑145 (MQ=255)
                                                          gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCCCACCCAAACCCACAC‑CCCAAACGGGCGAGGGGCCTGT‑CTGAGCCCA‑TTTTTa                                                                                            >  1:218371/1‑150 (MQ=255)
                                                              aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACttt                                                                                        <  1:1656356/150‑1 (MQ=255)
                                                              aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACttt                                                                                        <  2:2291314/150‑1 (MQ=255)
                                                                  ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGGAAATATGCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCa                                                                                    <  1:187728/150‑1 (MQ=255)
                                                                     aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCACACCCAAAACCACTA‑CCCAAAGGGGCGAGGGGACAGT‑CTGATGCCT‑TTTGTACATTGTCAtaa                                                                                 >  2:1548747/1‑148 (MQ=255)
                                                                      aTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCACAA‑CCCCAATGGGCGAGGGGACGGT‑CTGAGCACA‑TTTGTACTTTTTCATCTg                                                                                >  1:2121607/1‑150 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGa                                                                               <  2:3629190/150‑1 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGa                                                                               <  2:3224462/150‑1 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCCCCCCAACCCACTA‑CCCCAACGGGCGCGGGGACAGT‑CGGAGCCCT‑GTTGGACGTTGTCATCTGa                                                                               >  1:589162/1‑150 (MQ=255)
                                                                        gTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCACCCCCAAAACCACAC‑CCCCAAGGGGCGCGGGGGCTGT‑CTGTGCACA‑GTTGTTATTTGTGATATGAc                                                                              >  1:4461656/1‑150 (MQ=255)
                                                                          tGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGCCTGT‑CTGAGCACG‑TTTGTACTTTGTCATCTGACTa                                                                            >  2:360381/1‑150 (MQ=255)
                                                                          tGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTa                                                                            >  2:360595/1‑150 (MQ=255)
                                                                             cACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTaaaa                                                                         <  2:4449875/150‑1 (MQ=255)
                                                                              aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTaaaaa                                                                        <  1:519090/150‑1 (MQ=255)
                                                                               cGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAg                                                                       <  2:979564/150‑1 (MQ=255)
                                                                               cGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCGCACCCACAC‑CCCAAAGGGGCGCGGGGCCTGG‑CGGAGCACA‑TTTGTTCTTTTTCAGATGACaaaaaat                                                                       >  1:3699106/1‑149 (MQ=255)
                                                                                gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCGCTC‑CCCAAAGGGGCGGGGGGACTGG‑CTGAGCACG‑TTTGTACTTTGTCATATGACTAAAAAgg                                                                      >  2:3897327/1‑150 (MQ=25)
                                                                                  ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGgcg                                                                    <  1:3074323/150‑1 (MQ=255)
                                                                                   tttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCGAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACA‑TTTGTACTTTGTGATATGACAAAAAATgcgc                                                                   >  1:1209992/1‑150 (MQ=25)
                                                                                    ttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGcc                                                                  <  2:2401158/150‑1 (MQ=255)
                                                                                    ttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCCCCCAAACCCTCAC‑CCCCAAAGGGCGCGGGGGCGGG‑CTGTGCACG‑TTTGTACTTTTTGATCAGGATAAAAATGAGcc                                                                  >  1:3734795/1‑150 (MQ=255)
                                                                                    ttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATAGTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGcc                                                                  <  1:4105894/150‑1 (MQ=255)
                                                                                       ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa                                                               >  2:380304/1‑150 (MQ=255)
                                                                                       ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa                                                               <  1:5018257/150‑1 (MQ=255)
                                                                                          cTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTGTGTCATCTGACTAAAAAGGCGCCGAAgcg                                                            >  2:2160812/1‑150 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc                                                           <  1:4517823/150‑1 (MQ=255)
                                                                                              cccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCtt                                                        <  2:3401337/150‑1 (MQ=255)
                                                                                                cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGCGGGGCCGGT‑CTGAGCACT‑TTTGTACTTTGTCATATGACTAAAAATGCGCCGAAGCGCCGTTa                                                      >  2:3531854/1‑150 (MQ=37)
                                                                                                 tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                     <  1:2409865/150‑1 (MQ=255)
                                                                                                    cAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGCGGGGACTGT‑CGGAGCACT‑TTTGTACTTTGTCATCTGTCTAAAAAGGCGCCGAAGCGCCTTTAGaaa                                                  >  2:263221/1‑150 (MQ=255)
                                                                                                    cAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaa                                                  <  2:784935/150‑1 (MQ=255)
                                                                                                    cAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaa                                                  <  1:3949398/150‑1 (MQ=255)
                                                                                                     aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCAAACACACACACCCAAAGGGG‑GGGGGGGCAGT‑TTGAGCACG‑TGTGTAAGTTTTAAAAAGACTAAAAAAGAGCCGAACAGCCATAAGaaaa                                                 >  1:3671995/1‑150 (MQ=255)
                                                                                                      gggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAAt                                                <  1:4543599/150‑1 (MQ=255)
                                                                                                        gagaGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCCCAACCAAAAACACAA‑CCCAAATGGGCGAGGGGCCGGTGCTG‑GCACTATTTGT‑CATTGGCATCAGTCAAAAAAAGCGCCGGAGCGCCAGTAGgaaaaaa                                              >  2:879352/1‑147 (MQ=255)
                                                                                                         agagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGt                                             <  1:1121641/150‑1 (MQ=255)
                                                                                                             ggCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGaa                                         >  2:4667756/1‑150 (MQ=255)
                                                                                                                   ggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCACAC‑CCCAAAAGGGCGCGGGGACTGT‑GTGAGCACA‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCCAAGCGCCCTTAGAAAAAAGTCGAATCAGTg                                   >  2:1936335/1‑150 (MQ=255)
                                                                                                                    gTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGa                                  <  2:1990809/150‑1 (MQ=255)
                                                                                                                     tGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAATGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGaa                                 >  2:3235057/1‑150 (MQ=255)
                                                                                                                            aaaTATTCGCGCCAGTGCCGGCCTGGTCCCCGCACCCGAACCCTCTC‑CCCAAAGGGGCGCGGGGCCTGT‑CTGAGCACG‑TTTGTACCTTGTCATCTGACGAAAAAGGCGCCGAAGCGCCGTTAGAAAAAAGTCGAAGCAGTGAAGTACAgg                          >  2:4780807/1‑150 (MQ=37)
                                                                                                                                atTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACAC‑CCCAAAGGGGCGAGGGGGCGGT‑CTGAGCACT‑TGTGTTATTTGTCAACTGAATAAAAAGGCGCCGCAGCGCCTTTAGAAAATAGTCGCATCAGTGAATTTATGGTAtt                      >  2:1397187/1‑150 (MQ=37)
                                                                                                                                atTCGCGCCAGTGCCGGCCTGTTCCCCTAAACCAAACCCACAC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCAAA‑TTTGTACTTTTTCATCTGACAAAAAATGCGCCGAAGCGCCGGTATAAAATAGTCGAATAAGTGAAATAATGGGAta                      >  2:1025072/1‑149 (MQ=255)
                                                                                                                                  tCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCg                    <  2:1146827/150‑1 (MQ=255)
                                                                                                                                    gcgcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCt                  <  2:1833710/150‑1 (MQ=255)
                                                                                                                                    gcgcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCt                  <  1:3083065/150‑1 (MQ=255)
                                                                                                                                    gcgcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCt                  <  2:4890411/150‑1 (MQ=255)
                                                                                                                                     cgcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTa                 <  1:4679998/150‑1 (MQ=255)
                                                                                                                                       ccAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACAAAAAAGGCGCCGAAGCGCCTTTAGAAAAAAGTCGAATAAGTGAATTAATGGTATTCGCTAAt               >  2:3753707/1‑150 (MQ=255)
                                                                                                                                        cAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc              <  1:256355/150‑1 (MQ=255)
                                                                                                                                         aGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCACAGGGGCGAGGGGACGGT‑CCGAGCACC‑GATGTACTTTGTCATCTGACTACAAAGGCGCCGAAGCGCCATTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCg             <  2:5092515/150‑1 (MQ=38)
                                                                                                                                          gTGCCGGCCTGGTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCgg            <  2:1175615/150‑1 (MQ=255)
                                                                                                                                           tGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGt           <  2:499688/150‑1 (MQ=255)
                                                                                                                                           tGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGt           <  1:3331948/150‑1 (MQ=255)
                                                                                                                                            gCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTa          <  1:2282543/150‑1 (MQ=255)
                                                                                                                                                gCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCa      >  1:4575894/1‑150 (MQ=255)
                                                                                                                                                 ccTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg     >  1:1964743/1‑150 (MQ=255)
                                                                                                                                                 ccTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg     >  1:4639347/1‑150 (MQ=255)
                                                                                                                                                    tttCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAg  >  2:4248217/2‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTC‑CCCAAAGGGGCGAGGGGACTGT‑CTGAGCACT‑TTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAG  >  CP009273/3039260‑3039557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑