Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 255,487 A→T 100% S109T (TCT→ACT)  phoE ← outer membrane phosphoporin protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273255,4870AT99.6% 819.7 / ‑4.6 247S109T (TCT→ACT) phoEouter membrane phosphoporin protein E
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (119/126);  total (120/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 247 total aligned reads displayed.

CGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAA  >  CP009273/255340‑255632
                                                                                                                                                   |                                                                                                                                                 
cGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAc                                                                                                                                                 <  2:15556/150‑1 (MQ=255)
cGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAc                                                                                                                                                 <  1:98419/150‑1 (MQ=255)
 ggTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAcc                                                                                                                                                >  2:4769581/1‑150 (MQ=255)
  gtgtTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAccc                                                                                                                                               >  2:1773795/1‑150 (MQ=255)
    gtTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCaa                                                                                                                                             <  2:2353264/150‑1 (MQ=255)
      tCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAAt                                                                                                                                           <  1:1954306/150‑1 (MQ=255)
        cGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCt                                                                                                                                         <  1:1585406/150‑1 (MQ=255)
          aTACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCttt                                                                                                                                       <  1:1114455/150‑1 (MQ=255)
          aTACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCttt                                                                                                                                       <  2:4129810/150‑1 (MQ=255)
          aTACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCttt                                                                                                                                       <  2:1481318/150‑1 (MQ=255)
          aTACGTCGCCAGACCGCTGGCGAGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAATTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCttt                                                                                                                                       <  1:2945346/150‑1 (MQ=255)
            aCGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCACATCTTtat                                                                                                                                     >  2:3242991/1‑150 (MQ=255)
            aCGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtat                                                                                                                                     <  2:3891401/150‑1 (MQ=255)
             cGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtata                                                                                                                                    <  1:2265659/150‑1 (MQ=255)
              gTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTatat                                                                                                                                   >  2:219499/1‑150 (MQ=255)
                cGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATAttt                                                                                                                                 >  1:4541292/1‑150 (MQ=255)
                 gCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                >  2:3752401/1‑150 (MQ=255)
                 gCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                >  2:3355057/1‑150 (MQ=255)
                    aGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAc                                                                                                                             >  2:4458533/1‑150 (MQ=255)
                     gACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAcc                                                                                                                            <  2:1905246/150‑1 (MQ=255)
                      aCCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAccc                                                                                                                           <  1:4943558/150‑1 (MQ=255)
                        cGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCgg                                                                                                                         <  1:676889/150‑1 (MQ=255)
                           tGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaa                                                                                                                      >  1:5000347/1‑150 (MQ=255)
                            ggCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaaa                                                                                                                     <  1:5068971/150‑1 (MQ=255)
                                 gtctGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCga                                                                                                                >  2:1583164/4‑150 (MQ=255)
                                  tttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgag                                                                                                               >  1:3292427/1‑150 (MQ=255)
                                  tttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgag                                                                                                               <  2:654074/150‑1 (MQ=255)
                                    tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAc                                                                                                             >  2:1971067/1‑150 (MQ=255)
                                    tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAc                                                                                                             <  1:1261525/150‑1 (MQ=255)
                                    tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAc                                                                                                             <  1:795355/150‑1 (MQ=255)
                                        cATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGt                                                                                                         >  2:211723/1‑150 (MQ=255)
                                         aTAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtt                                                                                                        >  1:2351667/1‑150 (MQ=255)
                                          tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttt                                                                                                       <  2:4959497/150‑1 (MQ=255)
                                            aaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttttt                                                                                                     <  1:1905844/150‑1 (MQ=255)
                                            aaGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGttttt                                                                                                     <  2:200973/150‑1 (MQ=255)
                                             aGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtttttt                                                                                                    >  2:3172086/1‑150 (MQ=255)
                                              gTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTg                                                                                                   <  2:2947156/150‑1 (MQ=255)
                                                tGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCt                                                                                                 <  2:1315015/150‑1 (MQ=255)
                                                  tCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCtgt                                                                                               >  2:1744121/1‑150 (MQ=255)
                                                  tCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCtgt                                                                                               <  2:2753711/150‑1 (MQ=255)
                                                     gTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCa                                                                                            <  2:3383552/150‑1 (MQ=255)
                                                      tCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAg                                                                                           >  1:864971/1‑150 (MQ=255)
                                                             cGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCAct                                                                                    <  2:581362/150‑1 (MQ=255)
                                                              gAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActc                                                                                   >  2:936075/1‑150 (MQ=255)
                                                              gAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGAAGTATCActc                                                                                   >  2:936112/1‑150 (MQ=255)
                                                               aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActct                                                                                  >  1:66213/1‑150 (MQ=255)
                                                                        caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttat                                                                         >  2:1013713/1‑150 (MQ=255)
                                                                        caccaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttat                                                                         >  1:1976272/1‑150 (MQ=255)
                                                                          ccaAATTCCGGGAACATATCGGTCCAGGCTTCCACTTCATACAACGGCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCAGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttatta                                                                       <  1:3920672/150‑1 (MQ=255)
                                                                           caAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTAc                                                                      >  2:1122791/1‑150 (MQ=255)
                                                                             aaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCg                                                                    <  2:3806332/150‑1 (MQ=255)
                                                                               ttCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGc                                                                  <  1:1374629/150‑1 (MQ=255)
                                                                               ttCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACAGGc                                                                  >  2:2515606/1‑150 (MQ=255)
                                                                                 ccGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCac                                                                >  2:2817931/1‑149 (MQ=255)
                                                                                   gggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAAc                                                              <  1:1697015/150‑1 (MQ=255)
                                                                                   gggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAAc                                                              >  2:1033828/1‑150 (MQ=255)
                                                                                    ggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAAct                                                             <  2:3487728/150‑1 (MQ=255)
                                                                                      aaCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActct                                                           <  2:3233912/150‑1 (MQ=255)
                                                                                      aaCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActct                                                           >  1:2463175/1‑150 (MQ=255)
                                                                                        cATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGc                                                         <  2:1639674/150‑1 (MQ=255)
                                                                                         atatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCt                                                        >  1:1016288/1‑150 (MQ=255)
                                                                                         atatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCt                                                        >  1:4633578/1‑150 (MQ=255)
                                                                                          tatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCtt                                                       <  2:2173191/150‑1 (MQ=255)
                                                                                          tatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCtt                                                       >  1:359990/1‑150 (MQ=255)
                                                                                           atCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTc                                                      >  1:2577744/1‑150 (MQ=255)
                                                                                             cGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTccc                                                    <  2:4285115/150‑1 (MQ=255)
                                                                                              ggTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCa                                                   <  2:4181538/150‑1 (MQ=255)
                                                                                              ggTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCa                                                   >  1:4357876/1‑150 (MQ=255)
                                                                                                      cTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCAt                                           <  2:4686427/150‑1 (MQ=255)
                                                                                                       ttCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATa                                          >  2:240382/1‑150 (MQ=255)
                                                                                                         ccACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAAc                                        <  1:1125481/150‑1 (MQ=255)
                                                                                                          cACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAAcc                                       >  2:759263/1‑150 (MQ=255)
                                                                                                          cACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAAcc                                       <  2:1682119/150‑1 (MQ=255)
                                                                                                            cGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcag                                     <  1:2034771/150‑1 (MQ=255)
                                                                                                               cATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtca                                  <  1:870211/150‑1 (MQ=255)
                                                                                                                   cAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTg                              <  1:4259801/150‑1 (MQ=255)
                                                                                                                    aaCGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGa                             >  1:534011/1‑150 (MQ=255)
                                                                                                                      cGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATc                           >  1:1468203/1‑150 (MQ=255)
                                                                                                                      cGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATc                           <  2:482898/150‑1 (MQ=255)
                                                                                                                      cGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATc                           <  1:3339269/150‑1 (MQ=255)
                                                                                                                      cGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATc                           >  2:302884/1‑150 (MQ=255)
                                                                                                                      cGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATc                           <  2:4374177/150‑1 (MQ=255)
                                                                                                                         ccccAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGtt                        >  2:4572486/1‑150 (MQ=255)
                                                                                                                          cccAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCACACGACCATAACCAGTCAGTTGATctgta                       >  2:2410307/1‑146 (MQ=255)
                                                                                                                            cAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAt                     >  1:3464130/1‑150 (MQ=255)
                                                                                                                             aGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAtt                    >  1:2379366/1‑150 (MQ=255)
                                                                                                                              ggTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAttt                   >  2:2368381/1‑150 (MQ=255)
                                                                                                                               gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtg                  >  1:5041080/1‑150 (MQ=255)
                                                                                                                               gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtg                  <  2:5037245/150‑1 (MQ=255)
                                                                                                                                ttACGACCATAATCGAAAGTAACCAAATCTTTATATTTCAACCCGGCAACAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtgt                 <  2:5087432/150‑1 (MQ=255)
                                                                                                                                  aCGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTgtgt               >  1:871527/1‑150 (MQ=255)
                                                                                                                                  aCGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTgtgt               <  1:3712478/150‑1 (MQ=255)
                                                                                                                                     aCCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTc            >  1:4212952/1‑150 (MQ=255)
                                                                                                                                     aCCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTc            <  1:2820762/150‑1 (MQ=255)
                                                                                                                                      ccATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCg           <  1:3835361/150‑1 (MQ=255)
                                                                                                                                          aaTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCtt       >  2:1081386/1‑150 (MQ=255)
                                                                                                                                           aTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCttt      <  1:2991164/150‑1 (MQ=255)
                                                                                                                                            tCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTg     <  2:4236402/150‑1 (MQ=255)
                                                                                                                                            tCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTg     <  2:3292427/150‑1 (MQ=255)
                                                                                                                                               aaaGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGaaa  >  2:4499367/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAA  >  CP009273/255340‑255632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑