Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,041,321 G→T 100% I360I (ATC→ATA)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,041,3210GT97.5% 1139.6 / 13.0 363I360I (ATC→ATA) gcvPglycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base G (3/6);  new base T (153/201);  total (156/207)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 364 total aligned reads displayed.

TTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAT  >  CP009273/3041174‑3041469
                                                                                                                                                   |                                                                                                                                                    
 tCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCtata                                                                                                                                                   <  2:1809072/150‑1 (MQ=255)
  cAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATAc                                                                                                                                                  <  1:354729/150‑1 (MQ=255)
  cAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATAc                                                                                                                                                  <  1:281468/150‑1 (MQ=255)
      cGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGttt                                                                                                                                              >  1:4739916/1‑150 (MQ=255)
            ggCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGcc                                                                                                                                        <  2:3576404/150‑1 (MQ=255)
            ggCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGcc                                                                                                                                        <  2:1648549/150‑1 (MQ=255)
            ggCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGcc                                                                                                                                        <  2:2394856/150‑1 (MQ=255)
             gCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCa                                                                                                                                       <  1:5062646/150‑1 (MQ=255)
               aCGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAAc                                                                                                                                     <  2:2814923/150‑1 (MQ=255)
                cgcgTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAAcc                                                                                                                                    <  2:2449728/150‑1 (MQ=255)
                   gTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCggg                                                                                                                                 >  1:43943/1‑150 (MQ=255)
                     cAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGcc                                                                                                                               <  2:4368020/150‑1 (MQ=255)
                     cAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGcc                                                                                                                               <  1:4882521/150‑1 (MQ=255)
                      aGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCaa                                                                                                                                      <  1:1030549/142‑1 (MQ=255)
                      aGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCg                                                                                                                              <  1:2073375/150‑1 (MQ=255)
                       gTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGt                                                                                                                             >  2:3213601/1‑150 (MQ=255)
                       gTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGACCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGt                                                                                                                             <  1:1939260/150‑1 (MQ=255)
                         aCGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGa                                                                                                                           <  1:1123904/150‑1 (MQ=255)
                          cGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGAt                                                                                                                          >  1:5123069/1‑150 (MQ=255)
                          cGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGAt                                                                                                                          >  1:3342771/1‑150 (MQ=255)
                          cGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGAt                                                                                                                          <  1:3104719/150‑1 (MQ=255)
                           gCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATa                                                                                                                         >  1:886437/1‑150 (MQ=255)
                              cGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAAc                                                                                                                      >  1:4182212/1‑150 (MQ=255)
                                cTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTGGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGCATGCGGTTAGCTAGCCGTGTCAGGCCCACCGGGCCGTGCTCAACgg                                                                                                                    >  2:2948428/1‑150 (MQ=255)
                                     tCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATAc                                                                                                               >  2:903861/1‑150 (MQ=255)
                                       ggCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAg                                                                                                             >  1:575987/1‑150 (MQ=255)
                                       ggCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAg                                                                                                             >  1:3740324/1‑150 (MQ=255)
                                        gCCACTTCCACACACAAGGTGTCGAAATAGTTCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCATGCAAACCGGGCCGTGATAAACGGCATACAgg                                                                                                            >  1:3413822/1‑150 (MQ=255)
                                        gCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAgg                                                                                                            >  2:4418932/1‑150 (MQ=255)
                                         ccACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGc                                                                                                           >  2:4099307/1‑150 (MQ=255)
                                            cTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCGGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTgg                                                                                                        <  1:4899743/150‑1 (MQ=255)
                                              tCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCg                                                                                                      <  1:4072310/150‑1 (MQ=255)
                                               ccACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGa                                                                                                     <  1:236562/150‑1 (MQ=255)
                                               ccACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTCTCAGACCTTTTTGTTGCAGGACCGCCGCCAGGATATCGGTCAGACGGTGAATGAGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGa                                                                                                     >  2:1207876/1‑150 (MQ=255)
                                                cacacacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGAt                                                                                                    >  2:1437569/1‑150 (MQ=255)
                                                   acacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGtt                                                                                                 <  1:3871402/150‑1 (MQ=255)
                                                    cacaAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGttt                                                                                                <  1:2001220/150‑1 (MQ=255)
                                                        aGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCca                                                                                            <  2:594763/150‑1 (MQ=255)
                                                            gtCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCcagcag                                                                                        <  2:4761249/150‑1 (MQ=255)
                                                              cGAAATAGTGCGCATGGCTCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTa                                                                                      <  1:2992898/150‑1 (MQ=255)
                                                              cGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTa                                                                                      >  2:3487521/1‑150 (MQ=255)
                                                              cGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTa                                                                                      <  1:996312/150‑1 (MQ=255)
                                                              cGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTa                                                                                      >  2:2281888/1‑150 (MQ=255)
                                                                 aaTAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCt                                                                                   >  2:4628449/1‑150 (MQ=255)
                                                                 aaTAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCt                                                                                   >  1:4946622/1‑150 (MQ=255)
                                                                  aTAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTg                                                                                  <  1:173760/150‑1 (MQ=255)
                                                                   tAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTgg                                                                                 >  2:4524331/1‑150 (MQ=255)
                                                                     gTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGa                                                                               >  1:1845775/1‑150 (MQ=255)
                                                                        cgcATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGt                                                                            >  2:5115801/1‑150 (MQ=255)
                                                                               cgcAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATg                                                                     >  2:836237/1‑150 (MQ=255)
                                                                               cgcAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATg                                                                     >  1:1655096/1‑150 (MQ=255)
                                                                                  aGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTg                                                                  >  2:4715194/1‑150 (MQ=255)
                                                                                     ttCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAg                                                               <  1:4998435/150‑1 (MQ=255)
                                                                                     ttCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAg                                                               <  2:371128/150‑1 (MQ=255)
                                                                                      tCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGt                                                              <  2:3879397/150‑1 (MQ=255)
                                                                                       cAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGtt                                                             >  2:1944327/1‑150 (MQ=255)
                                                                                        aGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTc                                                            >  2:4308520/1‑150 (MQ=255)
                                                                                        aGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTc                                                            <  2:4546984/150‑1 (MQ=255)
                                                                                        aGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTc                                                            >  2:776539/1‑150 (MQ=255)
                                                                                        aGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTc                                                            >  1:1240939/1‑150 (MQ=255)
                                                                                        aGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTc                                                            <  2:117307/150‑1 (MQ=255)
                                                                                         gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCg                                                           <  1:244955/150‑1 (MQ=255)
                                                                                         gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCg                                                           <  2:1126582/150‑1 (MQ=255)
                                                                                         gACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCg                                                           >  2:3413094/1‑150 (MQ=255)
                                                                                           ccTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                         <  2:2906021/150‑1 (MQ=255)
                                                                                           ccTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                         <  2:3147248/150‑1 (MQ=255)
                                                                                             tttttGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCttt                                                       <  2:3773799/150‑1 (MQ=255)
                                                                                               tttGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                     <  1:822707/150‑1 (MQ=255)
                                                                                               tttGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtct                                                     <  1:2827584/150‑1 (MQ=255)
                                                                                                ttgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTtctc                                                    >  2:1057640/1‑150 (MQ=255)
                                                                                                 tgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCa                                                   >  1:2711606/1‑150 (MQ=255)
                                                                                                 tgttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCa                                                   >  1:4529193/1‑150 (MQ=255)
                                                                                                  gttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                  <  2:2221024/150‑1 (MQ=255)
                                                                                                   ttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcg                                                 <  2:1728988/150‑1 (MQ=255)
                                                                                                   ttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcg                                                 <  2:4005744/150‑1 (MQ=255)
                                                                                                   ttgCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcg                                                 >  1:2241094/1‑150 (MQ=255)
                                                                                                      cAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcg                                              <  1:4274516/150‑1 (MQ=255)
                                                                                                       aGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcgg                                             >  1:1526681/1‑150 (MQ=255)
                                                                                                        ggCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGa                                            <  2:4869448/150‑1 (MQ=255)
                                                                                                         gCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGat                                           <  1:3950885/150‑1 (MQ=255)
                                                                                                         gCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGat                                           <  1:4006645/150‑1 (MQ=255)
                                                                                                           ccgccgCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGatat                                         <  2:3967100/150‑1 (MQ=255)
                                                                                                             gccgccAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGACGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATCTGt                                       >  1:215996/1‑150 (MQ=255)
                                                                                                               cgccAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTg                                     <  2:4182212/150‑1 (MQ=255)
                                                                                                                gccAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGc                                    >  2:2127322/1‑150 (MQ=255)
                                                                                                                  cAGGATATCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                  <  2:4145665/150‑1 (MQ=255)
                                                                                                                        atCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                            >  1:5017367/1‑150 (MQ=255)
                                                                                                                        atCGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                            >  1:2892853/1‑150 (MQ=255)
                                                                                                                          cGGTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTca                          <  2:2543930/150‑2 (MQ=255)
                                                                                                                           ggTCAGACGGTGAATGCTGTTAGCTATACGTTTAAGGCCAACCGGGCCGTGATAAAAGTCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                         >  1:650357/1‑150 (MQ=255)
                                                                                                                           ggTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                         >  1:1322821/1‑150 (MQ=255)
                                                                                                                           ggTCAGACGGTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                         <  1:3260705/150‑1 (MQ=255)
                                                                                                                                  tggTGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGcc                  >  2:3006813/2‑150 (MQ=255)
                                                                                                                                   ggTGAATGCGGTGAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCa                 <  2:5076344/150‑1 (MQ=255)
                                                                                                                                     tGAATGCGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATg               >  2:3750064/1‑150 (MQ=255)
                                                                                                                                         tGCGGTTAGCTAGACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACACATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCa           <  1:4489032/150‑1 (MQ=255)
                                                                                                                                           cGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc         <  2:4045903/150‑1 (MQ=255)
                                                                                                                                           cGGTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc         <  2:1374521/150‑1 (MQ=255)
                                                                                                                                            ggTTAGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcg        <  2:1451290/150‑1 (MQ=255)
                                                                                                                                                aGCTATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGt    <  2:1924378/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAT  >  CP009273/3041174‑3041469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑