Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,618 T→A 100% V185E (GTG→GAG)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,6180TA99.6% 974.6 / ‑4.8 289V185E (GTG→GAG) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (127/160);  total (127/162)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 289 total aligned reads displayed.

CAGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCG  >  CP009273/3080471‑3080764
                                                                                                                                                   |                                                                                                                                                  
cAGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGc                                                                                                                                                  >  2:4964673/1‑150 (MQ=255)
 aGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCACAATCGTTGGTATCGATGCTGTCGAGCt                                                                                                                                                 >  2:3041010/1‑150 (MQ=255)
 aGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCt                                                                                                                                                 <  1:1970746/150‑1 (MQ=255)
     cctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACTGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTTACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTcc                                                                                                                                             >  1:410322/1‑150 (MQ=255)
     cctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTcc                                                                                                                                             <  2:3968964/150‑1 (MQ=255)
      ctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCa                                                                                                                                            >  2:1257788/1‑150 (MQ=255)
        atcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACt                                                                                                                                          <  2:3711826/150‑1 (MQ=255)
           acctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAg                                                                                                                                       <  1:3240513/150‑1 (MQ=255)
           acctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAg                                                                                                                                       <  1:1206490/150‑1 (MQ=255)
             ctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCa                                                                                                                                     >  1:1565356/1‑150 (MQ=255)
                tGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCActc                                                                                                                                  <  1:3986835/150‑1 (MQ=255)
                 gCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCActct                                                                                                                                 >  1:3187905/1‑150 (MQ=255)
                  cacaCCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTg                                                                                                                                <  2:4598680/150‑1 (MQ=255)
                   acacCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGa                                                                                                                               >  2:3310659/1‑150 (MQ=255)
                         tCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGAt                                                                                                                         >  1:708696/1‑150 (MQ=255)
                         tCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGAt                                                                                                                         >  1:1450470/1‑150 (MQ=255)
                          cTGGTACAGCGTCAGGCTGATGTGCGTAAAATCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATc                                                                                                                        <  1:170134/150‑1 (MQ=255)
                          cTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATc                                                                                                                        >  1:2619427/1‑150 (MQ=255)
                            ggTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGa                                                                                                                      <  1:3598497/150‑1 (MQ=255)
                             gTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGAc                                                                                                                     >  1:4645406/1‑150 (MQ=255)
                             gTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGAc                                                                                                                     <  1:3449561/150‑1 (MQ=255)
                              tACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGAcc                                                                                                                    >  2:4080314/1‑150 (MQ=255)
                              tACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGAcc                                                                                                                    <  1:1064326/150‑1 (MQ=255)
                               aCAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCa                                                                                                                   <  1:800072/150‑1 (MQ=255)
                                  gCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGaa                                                                                                                <  1:2503449/150‑1 (MQ=255)
                                    gTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAAt                                                                                                              >  1:3310272/1‑150 (MQ=255)
                                    gTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAAt                                                                                                              >  1:3573540/1‑150 (MQ=255)
                                      cAGGCTGAAGGGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCg                                                                                                            <  2:457798/150‑1 (MQ=255)
                                        ggCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCt                                                                                                          <  1:269049/150‑1 (MQ=255)
                                           tGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCa                                                                                                       >  1:672716/1‑150 (MQ=255)
                                           tGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCa                                                                                                       >  1:3737446/1‑150 (MQ=255)
                                              aGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaaga                                                                                                    <  1:1994186/150‑1 (MQ=255)
                                              aGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaaga                                                                                                    <  2:4339234/150‑1 (MQ=255)
                                                tGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaagaag                                                                                                  <  1:4492056/150‑1 (MQ=255)
                                                  cGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACTCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAATAGATCGACCAGAAATCGCTGCAAGAAGCg                                                                                                <  1:1690340/150‑1 (MQ=255)
                                                  cGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCg                                                                                                <  2:4133115/150‑1 (MQ=255)
                                                      aaaaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTaa                                                                                            <  1:512804/150‑1 (MQ=255)
                                                      aaaaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTaa                                                                                            <  1:4730262/150‑1 (MQ=255)
                                                       aaaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGTTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAAt                                                                                           >  2:1455123/1‑150 (MQ=255)
                                                        caCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATg                                                                                          <  1:3034486/149‑1 (MQ=255)
                                                           ggCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGaa                                                                                       >  2:1696190/1‑150 (MQ=255)
                                                            gCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAag                                                                                      <  2:5102085/150‑1 (MQ=255)
                                                              aCTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACAAGAAATCGCTGCAAGAAGCGGTAATGGAagag                                                                                    >  2:2432464/1‑150 (MQ=255)
                                                                 ctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGatc                                                                                 <  2:2066411/150‑1 (MQ=255)
                                                                     tgTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGAtcatca                                                                             >  1:4890900/2‑150 (MQ=255)
                                                                     cGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGAtcatca                                                                             >  1:4995400/1‑150 (MQ=255)
                                                                     cGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGAtcatca                                                                             >  2:2827343/1‑150 (MQ=255)
                                                                        ggCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGc                                                                          <  1:1833241/150‑1 (MQ=255)
                                                                         gCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGAAGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGcc                                                                         <  2:2633891/150‑1 (MQ=255)
                                                                          cTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCa                                                                        >  2:4840277/1‑150 (MQ=255)
                                                                            gcgcCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAAt                                                                      <  1:1419048/150‑1 (MQ=255)
                                                                             cgcCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAAtt                                                                     <  2:1170099/150‑1 (MQ=255)
                                                                               cccGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTCATGGAAGAGATCATCAAGCCAAGTCt                                                                   >  2:3913687/1‑150 (MQ=255)
                                                                               cccGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCt                                                                   >  1:8876/1‑150 (MQ=255)
                                                                                ccGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTg                                                                  <  1:5067417/150‑1 (MQ=255)
                                                                                 cGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACAAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCAGAAAGCaaaaaatga                                                                 >  2:2683909/1‑144 (MQ=255)
                                                                                      gcgAAAATCCAGTTGACTTTTCAGTATGACGACTGCAAAATCGTTGGTATAGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCt                                                            <  2:2611762/150‑1 (MQ=255)
                                                                                       cgAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTg                                                           <  1:2083160/150‑1 (MQ=255)
                                                                                       cgAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTg                                                           <  1:2166686/150‑1 (MQ=255)
                                                                                        gAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGa                                                          <  2:3680824/150‑1 (MQ=255)
                                                                                        gAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGa                                                          <  2:137337/150‑1 (MQ=255)
                                                                                          aaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAAt                                                        <  1:3916448/150‑1 (MQ=255)
                                                                                          aaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAAt                                                        <  1:4173121/150‑1 (MQ=255)
                                                                                          aaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAAt                                                        <  2:2319542/150‑1 (MQ=255)
                                                                                            aGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATgg                                                      >  2:2015633/1‑150 (MQ=255)
                                                                                             gCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGACCGCTGAATGGc                                                     >  2:4232934/1‑150 (MQ=255)
                                                                                               cAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTg                                                   >  2:4252781/1‑150 (MQ=255)
                                                                                               cAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTg                                                   <  2:4685967/150‑1 (MQ=255)
                                                                                                aGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGa                                                  >  2:2549455/1‑150 (MQ=255)
                                                                                                aGGTGAATTTTCTGTATGACGACGGCATAATCGTTGTTATCGATGCTGTCTAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGa                                                  <  1:2882543/150‑1 (MQ=255)
                                                                                                   tGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACtt                                               >  1:4281236/1‑150 (MQ=255)
                                                                                                    gACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTc                                              >  2:2999532/1‑150 (MQ=255)
                                                                                                        tttCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGcc                                          <  2:2101673/150‑1 (MQ=255)
                                                                                                         ttCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAGGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGaca                                         >  2:4755676/1‑150 (MQ=255)
                                                                                                         ttCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGcca                                         <  2:71750/150‑1 (MQ=255)
                                                                                                         ttCAGTATGACGACGGCAAAATCGTTGGTATCGATGCCGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGcca                                         >  1:1894307/1‑150 (MQ=255)
                                                                                                                   cgacgGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAAttctt                               <  1:4015949/150‑1 (MQ=255)
                                                                                                                     acgGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTtca                             <  1:4241557/150‑1 (MQ=255)
                                                                                                                       ggCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTtcatc                           >  1:2998750/1‑150 (MQ=255)
                                                                                                                       ggCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTtcatc                           >  2:2747466/1‑150 (MQ=255)
                                                                                                                          aaaaTCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAAc                        >  2:804447/1‑150 (MQ=255)
                                                                                                                            aaTCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAAccc                      <  1:255939/150‑1 (MQ=255)
                                                                                                                            aaTCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAAccc                      >  1:561899/1‑150 (MQ=255)
                                                                                                                               cGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGAc                   <  1:1319551/150‑1 (MQ=255)
                                                                                                                               cGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGAc                   >  1:352320/1‑150 (MQ=255)
                                                                                                                                 ttGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCg                 >  2:3601274/1‑150 (MQ=255)
                                                                                                                                   ggTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGt               <  1:94043/150‑1 (MQ=255)
                                                                                                                                    gTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtc              >  1:1299300/1‑150 (MQ=255)
                                                                                                                                     tATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcg             >  2:1524215/1‑150 (MQ=255)
                                                                                                                                     tATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcg             <  1:3788111/150‑1 (MQ=255)
                                                                                                                                     tATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcg             <  1:2374337/150‑1 (MQ=255)
                                                                                                                                       tCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtt           <  1:4847120/150‑1 (MQ=255)
                                                                                                                                       tCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtt           <  2:1861859/150‑1 (MQ=255)
                                                                                                                                       tCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtt           <  1:1640635/150‑1 (MQ=255)
                                                                                                                                       tCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCAGACCGGtcgtt           >  2:1620951/1‑150 (MQ=255)
                                                                                                                                         gATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgtttc         <  2:141345/150‑1 (MQ=255)
                                                                                                                                              tGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTAt    >  1:3700432/1‑150 (MQ=255)
                                                                                                                                               gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATc   <  1:1295276/150‑1 (MQ=255)
                                                                                                                                               gTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATc   <  2:4995400/150‑1 (MQ=255)
                                                                                                                                                tCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCg  <  2:3216355/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCG  >  CP009273/3080471‑3080764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑