Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,751 C→A 100% R574L (CGT→CTT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,7510CA100.0% 835.3 / NA 255R574L (CGT→CTT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (126/129);  total (126/129)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 257 total aligned reads displayed.

CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTAC  >  CP009273/3110604‑3110897
                                                                                                                                                   |                                                                                                                                                  
cTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGc                                                                                                                                                  >  2:4050257/1‑150 (MQ=255)
 tGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCa                                                                                                                                                 <  1:3574054/150‑1 (MQ=255)
     tttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAAt                                                                                                                                             >  1:854842/1‑150 (MQ=255)
     tttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAAt                                                                                                                                             <  1:2342270/150‑1 (MQ=255)
      ttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATg                                                                                                                                            <  2:3190257/150‑1 (MQ=255)
      ttCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATg                                                                                                                                            >  1:1261302/1‑150 (MQ=255)
         gcgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtg                                                                                                                                         >  1:1029241/1‑150 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                        <  2:2971830/150‑1 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                        <  2:2440399/150‑1 (MQ=255)
          cgTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgt                                                                                                                                        <  1:1115203/150‑1 (MQ=255)
           gTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgtt                                                                                                                                       >  2:443558/1‑150 (MQ=255)
           gTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCtgtt                                                                                                                                       >  2:109609/1‑150 (MQ=255)
              cGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTa                                                                                                                                    >  2:3286339/1‑150 (MQ=255)
                gCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCACAGCAAAATGCTGTTGTAgt                                                                                                                                  >  2:307789/1‑150 (MQ=255)
                 tttCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAgtg                                                                                                                                 >  2:574053/2‑150 (MQ=255)
                   tCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGtgtg                                                                                                                               <  1:4381012/150‑1 (MQ=255)
                    ccATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTgg                                                                                                                              <  2:4906639/150‑1 (MQ=255)
                    ccATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTgg                                                                                                                              >  1:1066445/1‑150 (MQ=255)
                    ccATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTgg                                                                                                                              >  1:561711/1‑150 (MQ=255)
                      aTTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATTAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                            <  2:3896257/150‑1 (MQ=255)
                      aTTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                            >  2:1441830/1‑150 (MQ=255)
                      aTTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGt                                                                                                                            <  2:730704/150‑1 (MQ=255)
                       tttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGtt                                                                                                                           <  1:294317/150‑1 (MQ=255)
                        ttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTa                                                                                                                          <  2:636263/150‑1 (MQ=255)
                        ttATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTa                                                                                                                          <  2:60421/150‑1 (MQ=255)
                          aTCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAcc                                                                                                                        <  2:3151135/150‑1 (MQ=255)
                          aTCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTAcc                                                                                                                        >  1:1223358/1‑150 (MQ=255)
                             gtcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGAt                                                                                                                     <  1:4840848/150‑1 (MQ=255)
                              tcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATa                                                                                                                    <  2:1041763/150‑1 (MQ=255)
                              tcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATa                                                                                                                    <  1:403387/150‑1 (MQ=255)
                              tcgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATa                                                                                                                    <  1:404060/150‑1 (MQ=255)
                               cgGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATaa                                                                                                                   >  1:3069834/1‑150 (MQ=255)
                                  aCAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCa                                                                                                                <  2:2337509/150‑1 (MQ=255)
                                      aTAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAg                                                                                                            <  2:2761597/150‑1 (MQ=255)
                                        aGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGtt                                                                                                          <  2:353929/150‑1 (MQ=255)
                                          cgcAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTg                                                                                                        <  1:4529031/150‑1 (MQ=255)
                                            cagcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGcc                                                                                                      <  2:907874/150‑1 (MQ=255)
                                            cagcaCGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGcc                                                                                                      >  1:4588718/1‑150 (MQ=255)
                                                     ctcCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGAc                                                                                             >  1:3117588/1‑150 (MQ=255)
                                                           gAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGccc                                                                                       >  2:3311460/1‑150 (MQ=255)
                                                                    cATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAAt                                                                              <  1:4401612/150‑1 (MQ=255)
                                                                    cATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAAt                                                                              >  1:3815940/1‑150 (MQ=255)
                                                                        tCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAAc                                                                          >  2:691387/1‑150 (MQ=255)
                                                                         cATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACg                                                                         <  1:1930040/150‑1 (MQ=255)
                                                                          aTCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACgg                                                                        >  1:1499889/1‑150 (MQ=255)
                                                                           tCCGGGTCGCTGTTGAGCGTAAACTGCCCATCTTTATTAACGCCCCAGTTCCAGCTGTAACAGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGc                                                                       <  1:2359987/150‑1 (MQ=255)
                                                                             cGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAg                                                                     >  1:3329372/1‑150 (MQ=255)
                                                                              gggTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAgg                                                                    >  1:4239749/1‑150 (MQ=255)
                                                                               ggTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGt                                                                   >  2:2471867/1‑150 (MQ=255)
                                                                                   gCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTaaa                                                               >  2:1880442/1‑150 (MQ=255)
                                                                                     tGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGGTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAgg                                                             >  2:5057772/1‑150 (MQ=255)
                                                                                      gTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGt                                                            >  1:4893506/1‑150 (MQ=255)
                                                                                       ttGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTg                                                           <  1:109609/150‑1 (MQ=255)
                                                                                         gAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGc                                                         <  1:2785842/150‑1 (MQ=255)
                                                                                          aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCg                                                        >  2:3120297/1‑150 (MQ=255)
                                                                                          aGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCg                                                        <  1:3956297/150‑1 (MQ=255)
                                                                                          aGCGTACACTGCCCATCTTGATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCg                                                        <  2:2074131/150‑1 (MQ=255)
                                                                                              tACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTAt                                                    >  1:3435679/1‑150 (MQ=255)
                                                                                               acacTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATc                                                   >  1:1760158/1‑150 (MQ=255)
                                                                                                  cTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcg                                                >  1:817162/1‑150 (MQ=255)
                                                                                                  cTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcg                                                <  2:2713820/150‑1 (MQ=255)
                                                                                                   tGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcgc                                               >  2:2180859/1‑150 (MQ=255)
                                                                                                    gCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATcgcgcg                                              >  2:4282050/1‑150 (MQ=255)
                                                                                                     cccATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGt                                             >  2:190738/1‑150 (MQ=255)
                                                                                                     cccATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGt                                             <  1:2672612/150‑1 (MQ=255)
                                                                                                      ccATCATTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATAAGTTTGCCCTCACCGACTAGCCCCAGCGAAAAAAACGGCAGGTTAAAGGTTGCTGTATCGCGCGTa                                            >  1:3376532/1‑150 (MQ=255)
                                                                                                         tCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAAca                                         <  1:3286339/150‑2 (MQ=255)
                                                                                                            ttattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGttt                                      >  2:1325648/1‑150 (MQ=255)
                                                                                                             tattaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGtttt                                     >  2:3976954/1‑150 (MQ=255)
                                                                                                              attaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTc                                    >  2:3840656/1‑150 (MQ=255)
                                                                                                               ttaACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTcc                                   <  2:1582325/150‑1 (MQ=255)
                                                                                                                taACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                  <  2:980641/150‑1 (MQ=255)
                                                                                                                taACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                  >  2:5013076/1‑150 (MQ=255)
                                                                                                                taACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                  >  2:5009003/1‑150 (MQ=255)
                                                                                                                taACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAACAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                  >  1:2145821/1‑150 (MQ=255)
                                                                                                                taACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAACAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCg                                  >  1:3500344/1‑150 (MQ=255)
                                                                                                                   cGCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCt                               <  1:4179584/150‑1 (MQ=255)
                                                                                                                    gCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                              <  1:2888220/150‑1 (MQ=255)
                                                                                                                    gCCCCCGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTc                              >  1:2975177/1‑150 (MQ=255)
                                                                                                                     cccccGTTCCAGCTGTAACCGTTCGGGAAAAGAAAAATGCTGTTGTAGTGTGGGTTACCTATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCTAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTca                             <  1:2001308/150‑1 (MQ=255)
                                                                                                                      ccccGTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTcaa                            >  1:2750262/1‑150 (MQ=255)
                                                                                                                          gTTCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCTGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAag                        <  1:574053/150‑1 (MQ=255)
                                                                                                                            tCCAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAagag                      >  2:3682553/1‑150 (MQ=255)
                                                                                                                             ccAGCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAgaga                     >  1:777824/1‑150 (MQ=255)
                                                                                                                                gCTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAgg                  >  1:3296725/1‑150 (MQ=255)
                                                                                                                                 cTGTAACCGTTCGGGCAAAGCAAAATGCTTTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgc                 <  2:1528430/150‑1 (MQ=255)
                                                                                                                                 cTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgc                 <  1:3008820/150‑1 (MQ=255)
                                                                                                                                 cTGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgc                 >  2:4411004/1‑150 (MQ=255)
                                                                                                                                  tGTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgcg                >  2:4953451/1‑150 (MQ=255)
                                                                                                                                   gTAACCGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGgcgc               >  1:1526356/1‑150 (MQ=255)
                                                                                                                                       ccGTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTcc           <  2:761590/150‑1 (MQ=255)
                                                                                                                                         gTTCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTACCGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGt         >  2:4226003/1‑150 (MQ=255)
                                                                                                                                          ttCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTa        >  2:518982/1‑150 (MQ=255)
                                                                                                                                          ttCGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGATTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTa        <  2:47057/150‑1 (MQ=255)
                                                                                                                                            cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt      >  2:3537681/1‑150 (MQ=255)
                                                                                                                                            cGGGCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAAt      >  1:495222/1‑150 (MQ=255)
                                                                                                                                             gggCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATg     <  1:274011/150‑1 (MQ=255)
                                                                                                                                              ggCAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGt    >  1:2064764/1‑150 (MQ=255)
                                                                                                                                               gcaaagcaaaATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTa   <  1:4282050/150‑1 (MQ=255)
                                                                                                                                                cAAAGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTAc  <  2:1773503/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGTCGCTGTTGAGCGTACACTGCCCATCTTTATTAACGCCCCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTAC  >  CP009273/3110604‑3110897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑