Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 279,334 C→A 100% I141I (ATC→ATA)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273279,3340CA98.2% 1294.2 / 7.3 411I141I (ATC→ATA) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base C (1/7);  new base A (203/198);  total (204/207)
Fisher's exact test for biased strand distribution p-value = 6.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 411 total aligned reads displayed.

CGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCT  >  CP009273/279187‑279480
                                                                                                                                                   |                                                                                                                                                  
cGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgt                                                                                                                                                  <  1:3661218/150‑1 (MQ=255)
cGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgt                                                                                                                                                  <  2:4484404/150‑1 (MQ=255)
cGGCAAGCAGATGCAGGCGGCGTCGAAGGAGATCACCCGCAATGGAGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGAACGCACGGTATGTTCGATACCCTGCCGTAACGCAACGACGCGGAGATAgt                                                                                                                                                  >  2:3506601/1‑150 (MQ=255)
 ggCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAgtg                                                                                                                                                 <  2:3833924/150‑1 (MQ=255)
   cATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGtt                                                                                                                                               >  2:692716/1‑150 (MQ=255)
     tgcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTc                                                                                                                                             >  2:1448602/1‑150 (MQ=255)
     tgcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTc                                                                                                                                             >  1:5017805/1‑150 (MQ=255)
      gcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcg                                                                                                                                            <  1:2245738/150‑1 (MQ=255)
      gcagatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGGTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcg                                                                                                                                            <  2:249595/150‑1 (MQ=255)
         gatgcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATACCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACTGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTcgcca                                                                                                                                         <  1:3892081/150‑2 (MQ=255)
           tgcagGCGGCGGCGAAGGAGATCACCCGCAAGGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCgccgcc                                                                                                                                       <  2:4420858/150‑1 (MQ=255)
            gcagGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCt                                                                                                                                      <  1:3715117/150‑1 (MQ=255)
                 cggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACTGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATcc                                                                                                                                 >  1:4166465/1‑150 (MQ=255)
                 cggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATcc                                                                                                                                 <  2:3952497/150‑1 (MQ=255)
                  ggcggcgAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCg                                                                                                                                >  1:4301557/1‑150 (MQ=255)
                        gAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCt                                                                                                                          >  1:885620/1‑150 (MQ=255)
                        gAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCt                                                                                                                          >  1:2711809/1‑150 (MQ=255)
                         aaGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTg                                                                                                                         >  2:2620219/1‑150 (MQ=255)
                            gagaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCg                                                                                                                      <  1:628060/150‑1 (MQ=255)
                            gagaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGAACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCg                                                                                                                      <  1:4774405/150‑1 (MQ=255)
                              gaTCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGAc                                                                                                                    <  1:2552744/150‑1 (MQ=255)
                                   cccGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACgcggc                                                                                                               >  1:1549902/1‑150 (MQ=255)
                                     cGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGcggcgg                                                                                                             <  1:60563/150‑1 (MQ=255)
                                        aaTGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCggcgggcg                                                                                                          <  1:542957/150‑1 (MQ=255)
                                         aTGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGgcgggcgg                                                                                                         >  1:4639835/1‑150 (MQ=255)
                                          tGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGt                                                                                                        >  1:2074724/1‑150 (MQ=255)
                                           ggCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTg                                                                                                       <  1:1522128/150‑1 (MQ=255)
                                             cGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGAt                                                                                                     >  2:898956/1‑150 (MQ=255)
                                              gggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATc                                                                                                    >  2:2390013/1‑150 (MQ=255)
                                               ggATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCg                                                                                                   <  2:4189462/150‑1 (MQ=255)
                                                gATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCgg                                                                                                  <  1:5084131/150‑1 (MQ=255)
                                                 aTCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGc                                                                                                 <  2:3010497/150‑1 (MQ=255)
                                                 aTCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGc                                                                                                 <  1:1344809/150‑1 (MQ=255)
                                                  tCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCg                                                                                                >  2:943778/1‑150 (MQ=255)
                                                   cccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGt                                                                                               >  1:2931989/1‑150 (MQ=255)
                                                    ccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTa                                                                                              >  2:2108256/1‑150 (MQ=255)
                                                    ccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTa                                                                                              >  2:220500/1‑150 (MQ=255)
                                                    ccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTa                                                                                              <  1:1730355/150‑1 (MQ=255)
                                                    ccGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTa                                                                                              <  1:2367734/150‑1 (MQ=255)
                                                      gTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                            >  2:3309150/1‑150 (MQ=255)
                                                      gTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                            >  1:4811133/1‑150 (MQ=255)
                                                      gTTCGCGGCCGTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCCCGCACGGTATGTTCGATTCCCTGCCGTACCGCACCGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGc                                                                                            <  2:2959024/150‑1 (MQ=255)
                                                       ttCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCg                                                                                           >  2:519110/1‑150 (MQ=255)
                                                          gcgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGAcc                                                                                        <  1:3570392/150‑1 (MQ=255)
                                                           cgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCt                                                                                       <  1:3243374/150‑1 (MQ=255)
                                                           cgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCt                                                                                       <  2:2921419/150‑1 (MQ=255)
                                                           cgGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCt                                                                                       >  2:4900698/1‑150 (MQ=255)
                                                               cTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGa                                                                                   >  2:1360912/1‑150 (MQ=255)
                                                               cTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGa                                                                                   <  2:3654532/150‑1 (MQ=255)
                                                                 tCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCTCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATa                                                                                 <  1:2425275/150‑1 (MQ=255)
                                                                  cGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaa                                                                                >  2:4550350/1‑150 (MQ=255)
                                                                   gTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                               <  1:3494784/150‑1 (MQ=255)
                                                                   gTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGAGTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATaaa                                                                               <  2:3269524/150‑1 (MQ=255)
                                                                    tCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAg                                                                              <  2:1481271/150‑1 (MQ=255)
                                                                        cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCt                                                                          >  1:2904246/1‑150 (MQ=255)
                                                                        cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCt                                                                          <  1:3352619/150‑1 (MQ=255)
                                                                        cGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCt                                                                          >  1:311420/1‑150 (MQ=255)
                                                                         gATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                         >  1:4680723/1‑150 (MQ=255)
                                                                         gATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTg                                                                         >  2:2039448/1‑150 (MQ=255)
                                                                           tCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGcc                                                                       >  2:1849029/1‑150 (MQ=255)
                                                                            ccGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTTTTTCGCCGCCTGATCCGCTCCCTGCCGTCGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGccc                                                                      <  1:1085160/150‑1 (MQ=255)
                                                                             cGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCGGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCg                                                                     <  2:3604948/150‑1 (MQ=255)
                                                                                gCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGcca                                                                  <  1:3415313/150‑1 (MQ=255)
                                                                                 cGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccac                                                                 >  2:82280/1‑150 (MQ=255)
                                                                                 cGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccac                                                                 <  2:1082767/150‑1 (MQ=255)
                                                                                 cGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccac                                                                 >  1:4836573/1‑150 (MQ=255)
                                                                                  gACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacc                                                                >  2:560256/1‑150 (MQ=255)
                                                                                  gACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacc                                                                >  2:559991/1‑150 (MQ=255)
                                                                                   aCGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGccacca                                                               <  2:2376617/150‑1 (MQ=255)
                                                                                     gggCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATg                                                             <  1:3532456/150‑1 (MQ=255)
                                                                                       gcgcTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGAt                                                           >  2:4430116/1‑150 (MQ=255)
                                                                                           tCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTgcg                                                       <  1:1396783/150‑1 (MQ=255)
                                                                                             gCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCt                                                     >  2:4325910/1‑150 (MQ=255)
                                                                                             gCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCt                                                     >  1:449343/1‑150 (MQ=255)
                                                                                              cAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTg                                                    >  2:2053118/1‑150 (MQ=255)
                                                                                                gggCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGc                                                  >  1:1784011/1‑150 (MQ=255)
                                                                                                  gcacgcacGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCcg                                                <  1:1935109/150‑1 (MQ=255)
                                                                                                   cacgcacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCcgc                                               <  2:2796240/150‑1 (MQ=255)
                                                                                                   cacgcacgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTACCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCcgc                                               >  1:1475994/1‑150 (MQ=255)
                                                                                                         cgGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGTTCTGCCCGCCACCATGATTGAGCTGGCCGCGATGCa                                         <  2:1240154/150‑1 (MQ=255)
                                                                                                            tATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCCTGATTGCGCTGGCCGCGATGCACGa                                      >  1:1001466/1‑150 (MQ=255)
                                                                                                              tGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGAcc                                    >  1:2575790/1‑150 (MQ=255)
                                                                                                               gTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCt                                   <  1:4564239/150‑1 (MQ=255)
                                                                                                                   gATTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCg                               <  2:4766054/150‑1 (MQ=255)
                                                                                                                    aTTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGa                              >  1:2526136/1‑150 (MQ=255)
                                                                                                                    aTTCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGa                              <  1:934280/150‑1 (MQ=255)
                                                                                                                      tCCCTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACt                            <  2:4646665/150‑1 (MQ=255)
                                                                                                                       cccTGCCGTACCGCAACGACGCGGCGATAGTGTGTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTa                           <  2:1733943/150‑1 (MQ=255)
                                                                                                                        ccTGCCGTACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTAt                          <  2:4990463/150‑1 (MQ=255)
                                                                                                                               tACCGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTg                   <  2:3184337/150‑1 (MQ=255)
                                                                                                                                  cGCAACGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcg                >  1:5111908/1‑150 (MQ=255)
                                                                                                                                     aaCGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggc             >  2:1964461/1‑150 (MQ=255)
                                                                                                                                     aaCGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggc             <  2:1589785/150‑1 (MQ=255)
                                                                                                                                     aaCGACGCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggc             <  1:3978976/150‑1 (MQ=255)
                                                                                                                                      acgacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcg            <  1:2830494/150‑1 (MQ=255)
                                                                                                                                        gacgCGGCGATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCcgggcggg          >  2:628578/1‑150 (MQ=255)
                                                                                                                                             ggcgATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGAc     >  2:2575657/1‑150 (MQ=255)
                                                                                                                                             ggcgATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGAc     <  1:759558/150‑1 (MQ=255)
                                                                                                                                             ggcgATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGAc     <  2:740805/150‑1 (MQ=255)
                                                                                                                                             ggcgATAGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGAc     <  1:1240764/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGCATGCAGATGCAGGCGGCGGCGAAGGAGATCACCCGCAATGGCGGGATCCCGTTCGCGGCCTTCGTCAGCGATCCGTGCGACGGGCGCTCGCAGGGCACGCACGGTATGTTCGATTCCCTGCCGTACCGCAACGACGCGGCGATCGTGTTTCGCCGCCTGATCCGCTCCCTGCCGACGCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCT  >  CP009273/279187‑279480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑