Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,184,211 C→A 94.4% S74Y (TCT→TAT)  yqiI → fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,184,2110CA94.4% 725.3 / 28.9 252S74Y (TCT→TAT) yqiIfimbrial protein
Reads supporting (aligned to +/- strand):  ref base C (8/6);  new base A (110/128);  total (118/134)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 254 total aligned reads displayed.

ATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAG  >  CP009273/3184064‑3184357
                                                                                                                                                   |                                                                                                                                                  
aTGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtatc                                                                                                                                                  >  1:4633267/1‑150 (MQ=255)
aTGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtatc                                                                                                                                                  >  2:2094043/1‑150 (MQ=255)
 tGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTcc                                                                                                                                                 >  2:1039349/1‑150 (MQ=255)
 tGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATcc                                                                                                                                                 <  1:2816419/150‑1 (MQ=255)
  gCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCa                                                                                                                                                <  2:3206169/150‑1 (MQ=255)
   cTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAg                                                                                                                                               >  2:860859/1‑150 (MQ=255)
   cTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAg                                                                                                                                               >  2:38576/1‑150 (MQ=255)
    tGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAgg                                                                                                                                              >  2:89333/1‑150 (MQ=255)
     gAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGga                                                                                                                                             >  2:4919871/1‑150 (MQ=255)
       aCATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGgaga                                                                                                                                           >  2:352824/1‑150 (MQ=255)
        cATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGagaa                                                                                                                                          <  1:3786204/150‑1 (MQ=255)
         aTGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGagaag                                                                                                                                         <  1:4897979/150‑1 (MQ=255)
           gAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAgaagaa                                                                                                                                       >  2:2397438/1‑150 (MQ=255)
           gAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAgaagaa                                                                                                                                       <  1:1886207/150‑1 (MQ=255)
            aaGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAAt                                                                                                                                      <  1:43101/150‑1 (MQ=255)
               gTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATAcc                                                                                                                                   >  1:2609588/1‑150 (MQ=255)
                tGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCACAGCAGGGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGACGAATAccc                                                                                                                                  >  2:3933279/1‑150 (MQ=255)
                    aaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCt                                                                                                                              <  1:3741974/150‑1 (MQ=255)
                     aTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTg                                                                                                                             <  2:1504656/150‑1 (MQ=255)
                       aCCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCa                                                                                                                           <  1:840457/150‑1 (MQ=255)
                        ccGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCAt                                                                                                                          <  1:4040377/150‑1 (MQ=255)
                          gTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATgg                                                                                                                        <  1:1039349/150‑1 (MQ=255)
                          gTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATgg                                                                                                                        >  1:4818619/1‑150 (MQ=255)
                           ttGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATggg                                                                                                                       <  1:1559417/150‑1 (MQ=255)
                               cATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCaaa                                                                                                                   >  2:2350125/1‑150 (MQ=255)
                                  tGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAATGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAatat                                                                                                                >  1:3538854/1‑150 (MQ=255)
                                   ggTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAAtata                                                                                                               <  2:4392676/150‑1 (MQ=255)
                                    gTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATaa                                                                                                              <  1:623262/150‑1 (MQ=255)
                                    gTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATaa                                                                                                              <  1:2392409/150‑1 (MQ=255)
                                             cAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGAtgttgt                                                                                                     <  1:2024811/150‑1 (MQ=255)
                                               aGAATCTCTAT‑CCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAg                                                                                                  <  2:1023451/150‑1 (MQ=255)
                                                 aaTCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGc                                                                                                 <  2:4542946/150‑1 (MQ=255)
                                                    ctctATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATc                                                                                              >  1:2115047/1‑150 (MQ=255)
                                                     tctATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCa                                                                                             <  2:1286651/150‑1 (MQ=255)
                                                       tATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAgg                                                                                           <  2:1429043/150‑1 (MQ=255)
                                                       tATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAgg                                                                                           <  2:1027049/150‑1 (MQ=255)
                                                        aTCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGt                                                                                          >  1:2694632/1‑150 (MQ=255)
                                                        aTCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGt                                                                                          >  2:4331592/1‑150 (MQ=255)
                                                         tCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTc                                                                                         <  2:4035036/150‑1 (MQ=255)
                                                           cccAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCaa                                                                                       >  1:2307590/1‑150 (MQ=255)
                                                              aGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGAc                                                                                    >  2:4200943/1‑150 (MQ=255)
                                                                cGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACtt                                                                                  <  2:4068493/150‑1 (MQ=255)
                                                                  aTGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACtttt                                                                                >  1:1013699/1‑150 (MQ=255)
                                                                   tGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTta                                                                               >  2:3356099/1‑150 (MQ=255)
                                                                    ggTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTtat                                                                              <  2:1934629/150‑1 (MQ=255)
                                                                       aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAAt                                                                           >  1:2182498/1‑150 (MQ=255)
                                                                        aaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATc                                                                          >  2:1698250/1‑150 (MQ=255)
                                                                         aaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCa                                                                         <  1:2994230/150‑1 (MQ=255)
                                                                         aaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCa                                                                         >  1:3429341/1‑150 (MQ=255)
                                                                          aaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaa                                                                        >  1:307925/1‑150 (MQ=255)
                                                                             ggTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAAc                                                                     <  1:1873405/150‑1 (MQ=255)
                                                                              gTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAAct                                                                    <  2:1918524/150‑1 (MQ=255)
                                                                                gATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                  <  2:2163563/150‑1 (MQ=255)
                                                                                gATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                  >  1:1765636/1‑150 (MQ=255)
                                                                                gATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                  >  1:3250486/1‑150 (MQ=255)
                                                                                  tAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTta                                                                >  2:3312356/1‑150 (MQ=255)
                                                                                    aaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtata                                                              <  2:2637269/150‑1 (MQ=255)
                                                                                    aaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtata                                                              <  1:4338944/150‑1 (MQ=255)
                                                                                    aaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtata                                                              <  2:1771221/150‑1 (MQ=255)
                                                                                    aaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtata                                                              <  2:523746/150‑1 (MQ=255)
                                                                                    aaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtata                                                              <  1:4119713/150‑1 (MQ=255)
                                                                                     aaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATAc                                                             >  1:417475/1‑150 (MQ=255)
                                                                                      aTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACt                                                            >  1:2790146/1‑150 (MQ=255)
                                                                                            cAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGgt                                                      >  1:5079277/1‑150 (MQ=255)
                                                                                              aaGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGgtgt                                                    >  2:4427187/1‑150 (MQ=255)
                                                                                               aGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTa                                                   >  1:3819089/1‑150 (MQ=255)
                                                                                                 cAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAAc                                                 >  2:80539/1‑150 (MQ=255)
                                                                                                   gTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACtt                                               <  1:1810537/150‑1 (MQ=255)
                                                                                                   gTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACtt                                               >  2:2761826/1‑150 (MQ=255)
                                                                                                     gAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTtat                                             <  2:4633267/150‑1 (MQ=255)
                                                                                                     gAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTtat                                             <  2:2976726/150‑1 (MQ=255)
                                                                                                        tatTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAAt                                          >  2:2285682/1‑150 (MQ=255)
                                                                                                         atTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATg                                         >  1:394993/1‑150 (MQ=255)
                                                                                                           tttATGTATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAATAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATggg                                       >  1:1296982/1‑150 (MQ=255)
                                                                                                             tATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGt                                     <  2:4152682/150‑1 (MQ=255)
                                                                                                              aTGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                    >  2:1380157/1‑150 (MQ=255)
                                                                                                                gAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGa                                  <  1:3681880/150‑1 (MQ=255)
                                                                                                                gAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGa                                  >  1:1729990/1‑150 (MQ=255)
                                                                                                                 aaTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAt                                 <  1:2468722/150‑1 (MQ=255)
                                                                                                                  aTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAtt                                <  1:565985/150‑1 (MQ=255)
                                                                                                                    gTTTTCTGTAATACTGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCATATATACTGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTAt                              <  2:3923038/150‑1 (MQ=255)
                                                                                                                     ttttCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATg                             <  2:219719/150‑1 (MQ=255)
                                                                                                                           gTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTa                       <  2:41781/150‑1 (MQ=255)
                                                                                                                            taataaTGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTAc                      <  1:1196779/150‑1 (MQ=255)
                                                                                                                             aataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACa                     >  1:927568/1‑150 (MQ=255)
                                                                                                                               taatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtc                   >  1:169674/1‑150 (MQ=255)
                                                                                                                                aatGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                  >  1:4650740/1‑150 (MQ=255)
                                                                                                                                  tGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTcc                >  2:4146396/1‑150 (MQ=255)
                                                                                                                                   gCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCa               <  2:3520541/150‑1 (MQ=255)
                                                                                                                                     aCTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACt             >  2:598172/1‑150 (MQ=255)
                                                                                                                                     aCTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACt             <  1:2630979/150‑1 (MQ=255)
                                                                                                                                       tACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTAc           >  2:3497839/1‑150 (MQ=255)
                                                                                                                                         ccAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCt         >  1:4679914/1‑150 (MQ=255)
                                                                                                                                         ccAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCt         <  1:1426350/150‑1 (MQ=255)
                                                                                                                                          cAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTa        <  2:1085455/150‑1 (MQ=255)
                                                                                                                                            ggCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTAtt      <  1:89320/150‑1 (MQ=255)
                                                                                                                                              cccATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGTTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATTGGGTTGATTATGATTCTACATCTCCACAACCTATTga    >  2:4819095/1‑149 (MQ=255)
                                                                                                                                              cccATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTgc    >  2:2837725/1‑150 (MQ=255)
                                                                                                                                               ccATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTgca   >  2:3804812/1‑150 (MQ=255)
                                                                                                                                               ccATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTgca   <  1:4751108/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAG  >  CP009273/3184064‑3184357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑