Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,205,512 A→C 10.8% L349L (CTA→CTC)  dnaG → DNA primase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,205,5120AC10.8% 810.5 / 89.5 336L349L (CTA→CTC) dnaGDNA primase
Reads supporting (aligned to +/- strand):  ref base A (155/144);  new base C (21/16);  total (176/160)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 344 total aligned reads displayed.

GCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTGAT  >  CP009273/3205363‑3205661
                                                                                                                                                     |                                                                                                                                                     
gcgACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTa                                                                                                                                                       <  1:1633063/150‑1 (MQ=255)
gcgACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTa                                                                                                                                                       <  1:1569183/150‑1 (MQ=255)
    ccAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTAc                                                                                                                                                   >  2:4767638/1‑150 (MQ=255)
      aacaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGa                                                                                                                                                 >  1:2561315/1‑150 (MQ=255)
      aacaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGa                                                                                                                                                 >  1:341399/1‑150 (MQ=255)
        caaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGaaa                                                                                                                                               >  2:2082907/1‑150 (MQ=255)
         aaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGaaaa                                                                                                                                              >  2:5134522/1‑150 (MQ=255)
           tGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAaaga                                                                                                                                            <  2:4569374/150‑1 (MQ=255)
           tGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAaaga                                                                                                                                            >  2:2310101/1‑150 (MQ=255)
           tGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAaaga                                                                                                                                            >  1:4619694/1‑150 (MQ=255)
             tCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAaagaag                                                                                                                                          >  1:3355837/1‑150 (MQ=255)
               aTTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGt                                                                                                                                        >  2:1878544/1‑150 (MQ=255)
                tttGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTa                                                                                                                                       >  1:713526/1‑150 (MQ=255)
                tttGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTa                                                                                                                                       >  2:3423133/1‑150 (MQ=255)
                 ttGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTaa                                                                                                                                      >  2:680123/1‑150 (MQ=255)
                  tGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTaaa                                                                                                                                     >  1:1258662/1‑150 (MQ=255)
                    cTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaaga                                                                                                                                   >  1:3648522/1‑150 (MQ=255)
                     tGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTATTGCCTGATTTCGAAGACCCTGACACGCTCTTACGAAAAGAATGTAaagaa                                                                                                                                  >  1:3417757/1‑150 (MQ=255)
                      gTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaagaag                                                                                                                                 >  2:2514002/1‑150 (MQ=255)
                      gTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaagaag                                                                                                                                 >  1:1326476/1‑150 (MQ=255)
                        tATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCg                                                                                                                               <  1:2623909/150‑1 (MQ=255)
                         aTGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGt                                                                                                                              >  1:2544268/1‑150 (MQ=255)
                          tGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGtt                                                                                                                             <  2:4072138/150‑1 (MQ=255)
                           gACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGttt                                                                                                                            >  1:2345620/1‑150 (MQ=255)
                            aCGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTg                                                                                                                           >  2:163202/1‑150 (MQ=255)
                               gCGACCGTGCTGGCCGCGATGCCGCGTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAg                                                                                                                        <  2:438014/150‑1 (MQ=255)
                               gCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAg                                                                                                                        <  1:534254/150‑1 (MQ=255)
                               gCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAg                                                                                                                        <  2:3348879/150‑1 (MQ=255)
                                 gACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                      >  1:646005/1‑150 (MQ=255)
                                 gACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                      <  2:271808/150‑1 (MQ=255)
                                 gACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                      >  1:4083941/1‑150 (MQ=255)
                                   ccGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGcgcg                                                                                                                    <  1:2744745/150‑1 (MQ=255)
                                   ccGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGcgcg                                                                                                                    <  2:154487/150‑1 (MQ=255)
                                    cGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGCCGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCgg                                                                                                                   >  2:1044488/1‑150 (MQ=255)
                                     gTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGa                                                                                                                  >  1:3467134/1‑150 (MQ=255)
                                     gTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGa                                                                                                                  <  2:3096833/150‑1 (MQ=255)
                                      tGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATTACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGAt                                                                                                                 <  1:1505675/150‑1 (MQ=255)
                                      tGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGAt                                                                                                                 >  1:4591366/1‑150 (MQ=255)
                                         aGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGa                                                                                                              <  2:77231/150‑1 (MQ=255)
                                             cgcgATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAg                                                                                                          <  1:820801/150‑1 (MQ=255)
                                             cgcgATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAg                                                                                                          >  1:4902229/1‑150 (MQ=255)
                                                gATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCg                                                                                                       >  1:2980614/1‑150 (MQ=255)
                                                 aTGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGa                                                                                                      >  2:2936264/1‑150 (MQ=255)
                                                  tGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGAt                                                                                                     >  1:2206258/1‑150 (MQ=255)
                                                     cgccTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGcc                                                                                                  >  2:2017807/1‑150 (MQ=255)
                                                     cgccTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGcc                                                                                                  <  2:3573857/150‑1 (MQ=255)
                                                     cgccTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGcc                                                                                                  <  1:3845094/150‑1 (MQ=255)
                                                       ccTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGGACGAAAAGAAGGTAAAGAATCGTTTGAAGCGCGGATGGAGCAGGCGATGCCAc                                                                                                >  2:51826/1‑150 (MQ=255)
                                                        cTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCAct                                                                                               >  1:103712/1‑150 (MQ=255)
                                                         tGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCActc                                                                                              <  2:4712893/150‑1 (MQ=255)
                                                         tGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCActc                                                                                              >  1:1530772/1‑150 (MQ=255)
                                                          ggCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCActct                                                                                             <  2:1245227/150‑1 (MQ=255)
                                                            cGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTcc                                                                                           <  2:502211/150‑1 (MQ=255)
                                                            cGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTcc                                                                                           >  1:3208174/1‑150 (MQ=255)
                                                             gAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCg                                                                                          <  2:2850324/150‑1 (MQ=255)
                                                             gAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCg                                                                                          <  1:2514319/150‑1 (MQ=255)
                                                               gcgcTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCa                                                                                        <  2:1365458/150‑1 (MQ=255)
                                                                  cTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCAttt                                                                                     <  1:2872657/150‑1 (MQ=255)
                                                                    ggAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCt                                                                                   <  2:2613468/150‑1 (MQ=255)
                                                                      aaaCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGt                                                                                 >  2:324501/1‑150 (MQ=255)
                                                                       aaCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGGACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGtt                                                                                <  2:4817946/150‑1 (MQ=255)
                                                                        aCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGttt                                                                               <  1:2944415/150‑1 (MQ=255)
                                                                         cGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTa                                                                              <  2:3154798/150‑1 (MQ=255)
                                                                          ggCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTaa                                                                             >  1:198994/1‑150 (MQ=255)
                                                                          ggCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTaa                                                                             <  1:180651/150‑1 (MQ=255)
                                                                            cgcTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACa                                                                           <  2:3477124/150‑1 (MQ=255)
                                                                                gCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCt                                                                       <  1:1092268/150‑1 (MQ=255)
                                                                                     aCATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGc                                                                  <  1:486070/150‑1 (MQ=255)
                                                                                     aCATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGc                                                                  <  1:140570/150‑1 (MQ=255)
                                                                                       aTGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCg                                                                <  2:4123648/150‑1 (MQ=255)
                                                                                        tGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGc                                                               >  1:4330807/1‑150 (MQ=255)
                                                                                        tGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGc                                                               <  2:3905072/150‑1 (MQ=255)
                                                                                          aCAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCaa                                                             <  2:4991472/150‑1 (MQ=255)
                                                                                              aCGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTg                                                         <  1:883414/150‑1 (MQ=255)
                                                                                                ggCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGAt                                                       >  1:4477296/1‑150 (MQ=255)
                                                                                                 gCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATc                                                      >  2:300919/1‑150 (MQ=255)
                                                                                                   cGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTg                                                    <  1:4767638/150‑1 (MQ=255)
                                                                                                   cGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTg                                                    >  2:3918193/1‑150 (MQ=255)
                                                                                                    gTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGa                                                   <  2:245799/150‑1 (MQ=255)
                                                                                                       aGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTa                                                <  2:3546173/150‑1 (MQ=255)
                                                                                                            cGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCt                                           <  1:741221/150‑1 (MQ=255)
                                                                                                             gCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTg                                          <  1:5141639/150‑1 (MQ=255)
                                                                                                               tttATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGAc                                        >  2:4508803/1‑150 (MQ=255)
                                                                                                               tttATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGAc                                        <  1:2412908/150‑1 (MQ=255)
                                                                                                                 tATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACgg                                      <  1:498533/150‑1 (MQ=255)
                                                                                                                  aTGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACggg                                     <  1:394264/150‑1 (MQ=255)
                                                                                                                   tGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGgc                                    >  2:4349096/1‑150 (MQ=255)
                                                                                                                             tGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGttt                          >  2:868714/1‑150 (MQ=255)
                                                                                                                              gATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTg                         >  1:4698000/1‑150 (MQ=255)
                                                                                                                                 ggCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGt                      <  2:3692589/150‑1 (MQ=255)
                                                                                                                                   cGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTAc                    >  1:428892/1‑150 (MQ=255)
                                                                                                                                   cGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTAc                    >  1:223168/1‑150 (MQ=255)
                                                                                                                                     aaGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGc                  <  1:3289011/150‑1 (MQ=255)
                                                                                                                                       gACCCTGACACGCGAGTACGAACAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGCTGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTg                <  2:1818917/150‑1 (MQ=255)
                                                                                                                                         cccTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGc              >  2:4269239/1‑150 (MQ=255)
                                                                                                                                         cccTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGc              >  2:1078635/1‑150 (MQ=255)
                                                                                                                                          ccTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCa             >  1:399940/1‑150 (MQ=255)
                                                                                                                                                 cGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCAt      >  2:628586/1‑150 (MQ=255)
                                                                                                                                                   cTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTg    <  1:4545492/150‑1 (MQ=255)
                                                                                                                                                    tAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTGa   >  1:1228159/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTGAT  >  CP009273/3205363‑3205661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑