Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,212,264 G→T 57.1% H58N (CAC→AAC)  aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,212,2640GT57.1% 42.4 / inf 310H58N (CAC→AAC) aerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base G (61/72);  new base T (87/90);  total (148/162)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 313 total aligned reads displayed.

TTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCT  >  CP009273/3212115‑3212410
                                                                                                                                                     |                                                                                                                                                  
   tCCCTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACg                                                                                                                                                 <  1:1491765/150‑1 (MQ=255)
    cccTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGc                                                                                                                                                >  2:3217163/1‑150 (MQ=255)
     ccTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCa                                                                                                                                               <  1:305065/150‑1 (MQ=255)
     ccTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCa                                                                                                                                               <  2:1706202/150‑1 (MQ=255)
        cgcgCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCa                                                                                                                                            >  2:619833/1‑150 (MQ=255)
         gcgcACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCAt                                                                                                                                           <  1:918584/150‑1 (MQ=255)
           gcACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCAtgt                                                                                                                                         >  1:2591555/1‑150 (MQ=255)
            cACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCAtgtt                                                                                                                                        <  2:3988757/150‑1 (MQ=255)
            cACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCAtgtt                                                                                                                                        >  2:3914483/1‑150 (MQ=255)
             aCCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCAtgttg                                                                                                                                       >  2:879559/1‑150 (MQ=255)
              ccATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCAtgttgt                                                                                                                                      >  1:569403/1‑150 (MQ=255)
               cATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTtgtg                                                                                                                                     >  1:1903849/1‑150 (MQ=255)
                 tCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCg                                                                                                                                   >  1:3225535/1‑150 (MQ=255)
                  tggTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCgg                                                                                                                                  >  1:2104849/2‑150 (MQ=255)
                  cGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCgg                                                                                                                                  >  1:1060623/1‑150 (MQ=255)
                    gTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCt                                                                                                                                <  2:785743/150‑1 (MQ=255)
                         gCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCt                                                                                                                           >  1:5126616/1‑150 (MQ=255)
                          cATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCtt                                                                                                                          <  1:4188837/150‑1 (MQ=255)
                           aTTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAGCGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTg                                                                                                                         <  1:3377962/150‑1 (MQ=255)
                           aTTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTg                                                                                                                         <  1:2729762/150‑1 (MQ=255)
                           aTTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTg                                                                                                                         >  2:1116181/1‑150 (MQ=255)
                            ttGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGt                                                                                                                        <  2:1825486/150‑1 (MQ=255)
                              ggCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTaa                                                                                                                      <  1:4520222/150‑1 (MQ=255)
                              ggCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTaa                                                                                                                      >  1:493276/1‑150 (MQ=255)
                              ggCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTaa                                                                                                                      >  2:5082368/1‑150 (MQ=255)
                                 ccGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAActc                                                                                                                   <  1:940304/150‑1 (MQ=255)
                                  cGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAActct                                                                                                                  <  2:3874029/150‑1 (MQ=255)
                                   gCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCtt                                                                                                                 <  2:3424901/150‑1 (MQ=255)
                                    cACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCACCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTg                                                                                                                >  1:1604581/1‑150 (MQ=255)
                                        cAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAg                                                                                                            >  2:408943/1‑150 (MQ=255)
                                        cAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAg                                                                                                            >  1:4167485/1‑150 (MQ=255)
                                         aataatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAgg                                                                                                           <  1:43303/150‑1 (MQ=255)
                                         aataatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAgg                                                                                                           >  2:4260490/1‑150 (MQ=255)
                                          ataatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGt                                                                                                          >  1:702253/1‑150 (MQ=255)
                                           taatGGTCACCATTTTTGCGGCGATTTTTCACGATGACGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCCTATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGta                                                                                                         >  2:551930/1‑150 (MQ=255)
                                            aatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGtat                                                                                                        >  1:3489003/1‑150 (MQ=255)
                                            aatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGtat                                                                                                        <  2:5061658/150‑1 (MQ=255)
                                            aatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTGTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGtat                                                                                                        <  1:5070342/150‑1 (MQ=255)
                                             atGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGtata                                                                                                       >  1:4461539/1‑150 (MQ=255)
                                              tGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAg                                                                                                      <  1:1796967/150‑1 (MQ=255)
                                                 tCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTg                                                                                                                         <  1:3190831/128‑1 (MQ=255)
                                                 tCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTg                                                                                                                         >  2:3190852/1‑128 (MQ=255)
                                                 tCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCg                                                                                                   <  1:4076497/150‑1 (MQ=255)
                                                  cACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGc                                                                                                  >  1:2450355/1‑150 (MQ=255)
                                                  cACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGc                                                                                                  >  1:2450600/1‑150 (MQ=255)
                                                       tttttGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCAcc                                                                                             >  2:3323987/1‑150 (MQ=255)
                                                                 gATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAg                                                                                   <  1:26951/150‑1 (MQ=255)
                                                                 gATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAg                                                                                   >  1:3359404/1‑150 (MQ=255)
                                                                  aTTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAgt                                                                                  <  1:3881679/150‑1 (MQ=255)
                                                                  aTTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAgt                                                                                  >  1:1607864/1‑150 (MQ=255)
                                                                      ttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCa                                                                              >  2:1020992/1‑150 (MQ=255)
                                                                       tCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCAt                                                                             >  1:4727064/1‑150 (MQ=255)
                                                                        cACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCCCAAAAGTGTCAtt                                                                            <  2:1747002/150‑1 (MQ=255)
                                                                        cACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCAtt                                                                            <  2:4320782/150‑1 (MQ=255)
                                                                        cACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCAtt                                                                            >  2:281055/1‑150 (MQ=255)
                                                                           gATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGc                                                                         >  2:3533062/1‑150 (MQ=255)
                                                                             tGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCAt                                                                       <  1:2881220/150‑1 (MQ=255)
                                                                              gCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGTCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATg                                                                      <  1:3729792/150‑1 (MQ=255)
                                                                               ccGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGa                                                                     >  1:1995670/1‑150 (MQ=255)
                                                                                cGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAg                                                                    >  2:1969781/1‑150 (MQ=255)
                                                                                cGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTCTAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAg                                                                    >  2:4535134/1‑150 (MQ=255)
                                                                                  cTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTg                                                                  >  2:3447489/1‑150 (MQ=255)
                                                                                     cAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGata                                                               <  2:1320999/150‑1 (MQ=255)
                                                                                       gggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGAtata                                                             <  1:4795858/150‑1 (MQ=255)
                                                                                        ggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGTATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAg                                                            <  2:2361824/150‑1 (MQ=255)
                                                                                            ccccTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCttt                                                        <  1:2845043/150‑1 (MQ=255)
                                                                                               cTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGct                                                     <  2:266419/150‑2 (MQ=255)
                                                                                                  ttttCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGAt                                                  <  1:1161450/150‑1 (MQ=255)
                                                                                                    ttCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCg                                                <  1:271757/150‑1 (MQ=255)
                                                                                                      cAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGt                                              >  2:2146007/1‑150 (MQ=255)
                                                                                                         ggTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTCCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGt                                           <  1:3237571/150‑1 (MQ=255)
                                                                                                          gTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTg                                          <  2:1846910/150‑1 (MQ=255)
                                                                                                           tGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTgg                                         <  2:3771601/150‑1 (MQ=255)
                                                                                                           tGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTgg                                         >  2:2270568/1‑150 (MQ=255)
                                                                                                            gAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGa                                        >  2:3173903/1‑150 (MQ=255)
                                                                                                               ccACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACAt                                     >  2:4787/1‑150 (MQ=255)
                                                                                                               ccACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACAt                                     <  1:1743617/150‑1 (MQ=255)
                                                                                                                  caTATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCag                                  <  1:4280673/150‑1 (MQ=255)
                                                                                                                   atatCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCaga                                 >  1:3697557/1‑150 (MQ=255)
                                                                                                                     ctCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGt                               <  1:4411095/149‑1 (MQ=255)
                                                                                                                     atCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGt                               >  1:3672705/1‑150 (MQ=255)
                                                                                                                       ccGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTgg                             >  1:4635415/1‑150 (MQ=255)
                                                                                                                        cGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGt                            <  2:4880578/150‑1 (MQ=255)
                                                                                                                          cAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTAt                          >  2:1239374/1‑150 (MQ=255)
                                                                                                                           aaaCGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATc                         >  1:1288996/1‑150 (MQ=255)
                                                                                                                            aaCGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCg                        >  2:2847621/1‑150 (MQ=255)
                                                                                                                              cgccgcTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTACCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTc                      >  1:1658959/1‑150 (MQ=255)
                                                                                                                               gccgcTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTcc                     <  1:3737368/150‑1 (MQ=255)
                                                                                                                                ccgcTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCg                    <  2:1549883/150‑1 (MQ=255)
                                                                                                                                      ttGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCg              >  2:1432606/1‑150 (MQ=255)
                                                                                                                                        ggCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCggg            >  2:1518434/1‑150 (MQ=255)
                                                                                                                                         gCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCgggg           <  1:4991865/150‑1 (MQ=255)
                                                                                                                                          cATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGt          >  1:5040546/1‑150 (MQ=255)
                                                                                                                                          cATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGt          <  2:4474726/150‑1 (MQ=255)
                                                                                                                                            tatCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAt        <  1:2481970/150‑1 (MQ=255)
                                                                                                                                            tatCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAt        >  1:228708/1‑150 (MQ=255)
                                                                                                                                             atCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAtt       <  2:4727064/150‑1 (MQ=255)
                                                                                                                                             atCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAtt       <  1:4939314/150‑1 (MQ=255)
                                                                                                                                              tCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAttt      <  2:812181/150‑1 (MQ=255)
                                                                                                                                              tCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAttt      >  2:1837571/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCT  >  CP009273/3212115‑3212410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑