Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 279,800 G→T 100% E297* (GAA→TAA)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273279,8000GT99.2% 1284.0 / ‑0.4 389E297* (GAA→TAA) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (180/205);  total (183/206)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 391 total aligned reads displayed.

AGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTG  >  CP009273/279651‑279949
                                                                                                                                                     |                                                                                                                                                     
  gcgcTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTaa                                                                                                                                                     >  2:125956/1‑150 (MQ=255)
   cgcTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTaaa                                                                                                                                                    <  1:1008107/150‑1 (MQ=255)
    gcTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTaaaa                                                                                                                                                   <  2:3994686/150‑1 (MQ=255)
         gTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACgaga                                                                                                                                              <  1:1429010/150‑1 (MQ=255)
         gTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGa                                                                                                                                              >  2:4124514/1‑150 (MQ=255)
          tCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGAt                                                                                                                                             <  1:2108182/150‑1 (MQ=255)
           cTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATg                                                                                                                                            <  1:3100293/150‑1 (MQ=255)
           cTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATg                                                                                                                                            <  1:4193603/150‑1 (MQ=255)
             ggCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGt                                                                                                                                          >  1:1865995/1‑150 (MQ=255)
             ggCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGt                                                                                                                                          >  1:2057542/1‑150 (MQ=255)
              gcgcTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGTGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTg                                                                                                                                         <  2:2827020/150‑1 (MQ=255)
                gcTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGAGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGTTGGTGAt                                                                                                                                       <  2:2232285/150‑1 (MQ=255)
                 cTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATc                                                                                                                                      <  2:654181/150‑1 (MQ=255)
                  tGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATcc                                                                                                                                     >  2:1079804/1‑150 (MQ=255)
                     cGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGAGAAAGCCATCGAAAACGCGATGGTGATCCAcg                                                                                                                                  <  1:3048977/150‑1 (MQ=255)
                       cACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCAcgcg                                                                                                                                >  2:3908597/1‑150 (MQ=255)
                        aCTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTCAAACGCGATGGTGATCCACgcgg                                                                                                                               >  2:5041863/1‑150 (MQ=255)
                        aCTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcgg                                                                                                                               >  1:1653038/1‑150 (MQ=255)
                         cTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGTCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGATCTTGATAGCCGCGGCATCTCCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggc                                                                                                                              <  1:4908052/150‑1 (MQ=255)
                         cTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggc                                                                                                                              >  2:4173681/1‑150 (MQ=255)
                          tCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACgcggcg                                                                                                                             <  1:1996593/150‑1 (MQ=255)
                            cgcgCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGtt                                                                                                                           <  2:129681/150‑1 (MQ=255)
                             gcgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCCCGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTc                                                                                                                          <  1:3526807/150‑1 (MQ=255)
                              cgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTGcg                                                                                                                         >  2:3605151/1‑150 (MQ=255)
                                     gcCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTc                                                                                                                  <  1:4950235/150‑1 (MQ=255)
                                     gcCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTc                                                                                                                  >  2:3506219/1‑150 (MQ=255)
                                       cGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTcca                                                                                                                <  1:4012146/150‑1 (MQ=255)
                                        gTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTccac                                                                                                               >  1:2957837/1‑150 (MQ=255)
                                           cGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCaa                                                                                                            <  1:2992971/150‑1 (MQ=255)
                                           cGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCaa                                                                                                            <  2:4754930/150‑1 (MQ=255)
                                            gggCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAAt                                                                                                           <  1:2388302/150‑1 (MQ=255)
                                               cAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTa                                                                                                        >  1:575140/1‑150 (MQ=255)
                                                aGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTAc                                                                                                       >  2:1355804/1‑150 (MQ=255)
                                                  gCGGTGTGTCTTGATATCGCCCGCCAGTCGGCGCGCTCGGTCAGCGAGCTTGATAGCCGCGGCATCACCAAGCGGGTTATCCTCTCCGATAAAGCCATATATAACGCGATGGTGATCAAAGCGGCGTTCGGCGGCTCCACCAATTTActg                                                                                                     <  1:2502327/150‑1 (MQ=255)
                                                      tgtgGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACtgctgc                                                                                                 <  1:4593003/150‑1 (MQ=255)
                                                       gtgGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGca                                                                                                >  1:1399017/1‑150 (MQ=255)
                                                            tGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTc                                                                                           <  2:3397642/150‑1 (MQ=255)
                                                             ggAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTcc                                                                                          <  1:25877/150‑1 (MQ=255)
                                                             ggAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTcc                                                                                          >  2:2565051/1‑150 (MQ=255)
                                                              gagaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCg                                                                                         <  1:646512/150‑1 (MQ=255)
                                                               agaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCgg                                                                                        <  1:333492/150‑1 (MQ=255)
                                                               agaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGAGAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACAGTCCgg                                                                                        <  2:968987/150‑1 (MQ=255)
                                                                gaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGc                                                                                       >  2:894663/1‑150 (MQ=255)
                                                                gaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGc                                                                                       >  2:2978790/1‑150 (MQ=255)
                                                                 aTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGcc                                                                                      <  1:2882858/150‑1 (MQ=255)
                                                                   cgcccgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCAt                                                                                    >  1:906973/1‑150 (MQ=255)
                                                                    gcccgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCAATCTACAACGCGATGGTGATCCACGCGGCGTGCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATc                                                                                   >  1:479925/1‑150 (MQ=255)
                                                                     cccgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGTTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCg                                                                                  <  1:502397/150‑1 (MQ=255)
                                                                     cccgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCg                                                                                  <  1:3642918/150‑1 (MQ=255)
                                                                       cgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGcc                                                                                >  2:3254156/1‑150 (MQ=255)
                                                                         ccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCa                                                                              <  2:4469947/150‑1 (MQ=255)
                                                                           aGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCGGGCCATCGCCCAcg                                                                            <  2:2790853/150‑1 (MQ=255)
                                                                              cGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACgcgg                                                                         >  2:2447855/1‑150 (MQ=255)
                                                                                gcgcgcgcGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACgcggcg                                                                       <  1:3908469/150‑1 (MQ=255)
                                                                                    gcgcgGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCt                                                                   <  1:2046402/150‑1 (MQ=255)
                                                                                    gcgcgGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCt                                                                   <  1:1777599/150‑1 (MQ=255)
                                                                                           cAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGAt                                                            >  2:1956552/1‑150 (MQ=255)
                                                                                           cAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGAt                                                            >  2:2056055/1‑150 (MQ=255)
                                                                                              cGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATccc                                                         <  1:2781704/150‑1 (MQ=255)
                                                                                              cGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATccc                                                         >  1:4048934/1‑150 (MQ=255)
                                                                                               gAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATGTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCg                                                        <  2:2695833/150‑1 (MQ=255)
                                                                                                  cTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGAc                                                     >  2:532313/1‑150 (MQ=255)
                                                                                                   tGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACg                                                    >  1:4710244/1‑150 (MQ=255)
                                                                                                   tGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACg                                                    >  1:3424689/1‑150 (MQ=255)
                                                                                                     gATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGtt                                                  >  2:4895831/1‑150 (MQ=255)
                                                                                                      aTAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTg                                                 >  1:4288366/1‑150 (MQ=255)
                                                                                                       tAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGa                                                <  2:1514043/150‑1 (MQ=255)
                                                                                                       tAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGa                                                >  1:2925968/1‑150 (MQ=255)
                                                                                                         gCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGc                                              <  1:713262/150‑1 (MQ=255)
                                                                                                          ccGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCa                                             <  1:192674/150‑1 (MQ=255)
                                                                                                          ccGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCa                                             <  2:2738723/150‑1 (MQ=255)
                                                                                                          ccGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCa                                             <  1:1496839/150‑1 (MQ=255)
                                                                                                             cgGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTg                                          >  1:3240164/1‑150 (MQ=255)
                                                                                                                taTCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGAc                                       >  2:3849745/2‑150 (MQ=255)
                                                                                                                 aTCACCACGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGAcg                                      <  2:1609271/150‑1 (MQ=255)
                                                                                                                   caccacGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGAcgcg                                    <  1:537768/150‑1 (MQ=255)
                                                                                                                    accacGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACgcgc                                   >  2:4763531/1‑150 (MQ=255)
                                                                                                                     ccacGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCa                                  >  2:743609/1‑150 (MQ=255)
                                                                                                                      cacGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCAt                                 <  2:1227119/150‑1 (MQ=255)
                                                                                                                      cacGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCAt                                 >  1:1144644/1‑150 (MQ=255)
                                                                                                                      cacGCGGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCAt                                 >  2:4852907/1‑150 (MQ=255)
                                                                                                                        cgcgGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCa                               <  1:4099884/150‑1 (MQ=255)
                                                                                                                          cgGGATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAc                             >  2:5154294/1‑150 (MQ=255)
                                                                                                                           gggATATCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAcc                            <  1:125956/150‑1 (MQ=255)
                                                                                                                              atatCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTa                         <  2:2920943/150‑1 (MQ=255)
                                                                                                                              atatCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTa                         <  2:4525575/150‑1 (MQ=255)
                                                                                                                               tatCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTaa                        >  1:1353177/1‑150 (MQ=255)
                                                                                                                                 tCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAg                      <  1:3729675/150‑1 (MQ=255)
                                                                                                                                 tCCTCTCCGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAg                      <  2:2489812/150‑1 (MQ=255)
                                                                                                                                        cGATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCcgcg               <  1:1079759/150‑1 (MQ=255)
                                                                                                                                         gATAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGt              <  2:4292689/150‑1 (MQ=255)
                                                                                                                                          aTAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTc             >  2:4681160/1‑150 (MQ=255)
                                                                                                                                          aTAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTc             >  1:4646490/1‑150 (MQ=255)
                                                                                                                                          aTAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTc             >  2:2865982/1‑150 (MQ=255)
                                                                                                                                           tAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCt            >  1:1530261/1‑150 (MQ=255)
                                                                                                                                           tAAAGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCt            <  2:1816245/150‑1 (MQ=255)
                                                                                                                                            aaaGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTg           >  1:3973308/1‑150 (MQ=255)
                                                                                                                                            aaaGCCATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTg           <  2:2349279/150‑1 (MQ=255)
                                                                                                                                                ccATCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGa       >  2:820745/1‑150 (MQ=255)
                                                                                                                                                  aTCTAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGc     <  2:3020799/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTG  >  CP009273/279651‑279949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑