Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,238,283 C→A 100% intergenic (‑183/‑180) uxaC ← / → exuT uronate isomerase/hexuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,238,2830CA99.3% 926.7 / ‑3.3 278intergenic (‑183/‑180)uxaC/exuTuronate isomerase/hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (164/112);  total (165/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 286 total aligned reads displayed.

TATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAA  >  CP009273/3238136‑3238429
                                                                                                                                                   |                                                                                                                                                  
tATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAg                                                                                                                                                  >  2:4539553/1‑150 (MQ=255)
  tGGTGCGATGCTGATAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGt                                                                                                                                                <  2:1551171/150‑1 (MQ=255)
     tGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAAt                                                                                                                                             <  2:5111162/150‑1 (MQ=255)
      gCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATg                                                                                                                                            <  1:3591153/150‑1 (MQ=255)
        gATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTc                                                                                                                                          >  2:2129172/1‑150 (MQ=255)
        gATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTc                                                                                                                                          >  1:4935994/1‑150 (MQ=255)
          tGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTccc                                                                                                                                        <  1:3783704/150‑1 (MQ=255)
           gCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCt                                                                                                                                       <  1:4026242/150‑1 (MQ=255)
            cTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTa                                                                                                                                      <  1:4082548/150‑1 (MQ=255)
            cTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTa                                                                                                                                      >  2:2771686/1‑150 (MQ=255)
                  aaGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAata                                                                                                                                >  1:539918/1‑150 (MQ=255)
                   aGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAatat                                                                                                                               >  2:3517174/1‑150 (MQ=255)
                      tATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTcc                                                                                                                            >  1:1231159/1‑150 (MQ=255)
                        tCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCca                                                                                                                          <  1:3855376/150‑1 (MQ=255)
                        tCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCca                                                                                                                          <  2:2171227/150‑1 (MQ=255)
                         cacaCCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCcac                                                                                                                         >  2:1160484/1‑150 (MQ=255)
                             ccAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACAttt                                                                                                                     <  2:4706518/150‑1 (MQ=255)
                             ccAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACAttt                                                                                                                     <  1:2387502/150‑1 (MQ=255)
                                aTTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTtgtg                                                                                                                  <  1:4050135/150‑1 (MQ=255)
                                   tCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATg                                                                                                               <  2:2381396/150‑1 (MQ=255)
                                    ccAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATgg                                                                                                              >  1:5096915/1‑150 (MQ=255)
                                    ccAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGAGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATgg                                                                                                              >  2:365103/1‑150 (MQ=255)
                                     cAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGc                                                                                                             >  1:2869142/1‑150 (MQ=255)
                                         gTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc                                                                                                         >  1:2344975/1‑150 (MQ=255)
                                         gTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc                                                                                                         <  2:5076962/150‑1 (MQ=255)
                                         gTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc                                                                                                         >  1:1812168/1‑150 (MQ=255)
                                            cGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc                                                                                                      >  1:3421147/1‑150 (MQ=255)
                                            cGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc                                                                                                      <  1:4260307/150‑1 (MQ=255)
                                             gAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGTCTCTCACCt                                                                                                     >  1:4331023/1‑150 (MQ=255)
                                             gAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt                                                                                                     >  2:1796345/1‑150 (MQ=255)
                                             gAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt                                                                                                     >  2:4369502/1‑150 (MQ=255)
                                              aaGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtt                                                                                                    <  1:4284304/150‑1 (MQ=255)
                                                  tatTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTaa                                                                                                >  2:1771847/1‑150 (MQ=255)
                                                   atTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTaaa                                                                                               >  1:1713420/1‑150 (MQ=255)
                                                      ttCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGtt                                                                                            >  2:1010560/1‑150 (MQ=255)
                                                       tctttagttagttCAATAAACGTATTTAAAAAAATTACTCTCAAAGTTGTAAATCTCGCTGCTGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACATATATTCCCCCATTTGTGATGGCTCTCACCTTTTAAAtttt                                                                                           <  1:2355837/145‑2 (MQ=255)
                                                       tCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACACATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg                                                                                           >  1:3364430/1‑150 (MQ=255)
                                                       tCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg                                                                                           <  2:84547/150‑1 (MQ=255)
                                                         gTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTa                                                                                         >  1:3676469/1‑150 (MQ=255)
                                                           gAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATg                                                                                       <  2:3715463/150‑1 (MQ=255)
                                                           gAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATg                                                                                       <  1:2214424/150‑1 (MQ=255)
                                                            aGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGa                                                                                      >  2:4185436/1‑150 (MQ=255)
                                                              ttAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACa                                                                                    >  2:1830951/1‑150 (MQ=255)
                                                               tAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACaa                                                                                   >  2:4050147/1‑150 (MQ=255)
                                                                  aTCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGtt                                                                                >  2:451573/1‑150 (MQ=255)
                                                                    cAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt                                                                              >  1:4259277/1‑150 (MQ=255)
                                                                      aTAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCt                                                                            <  1:2130034/150‑1 (MQ=255)
                                                                      aTAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTAAtat                                                                            >  1:1384280/1‑148 (MQ=255)
                                                                      aTAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCACTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCt                                                                            >  1:3963449/1‑150 (MQ=255)
                                                                       tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                                                                           >  2:1970714/1‑150 (MQ=255)
                                                                       tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                                                                           >  2:2196060/1‑150 (MQ=255)
                                                                       tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                                                                           >  1:2998462/1‑150 (MQ=255)
                                                                        aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCttt                                                                          >  1:1929932/1‑150 (MQ=255)
                                                                          aCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCt                                                                        <  2:4922370/150‑1 (MQ=255)
                                                                          aCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCt                                                                        >  2:3609073/1‑150 (MQ=255)
                                                                            gTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGc                                                                      >  1:594770/1‑150 (MQ=255)
                                                                            gTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGc                                                                      >  2:2000482/1‑150 (MQ=255)
                                                                             tAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGcc                                                                     <  2:630048/150‑1 (MQ=255)
                                                                                     aaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCaaa                                                             <  1:4471693/150‑1 (MQ=255)
                                                                                       aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATc                                                           <  2:2532939/150‑1 (MQ=255)
                                                                                         ttACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCAt                                                         >  2:2408802/1‑150 (MQ=255)
                                                                                             tctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt                                                     >  2:1248050/1‑150 (MQ=255)
                                                                                                 aaaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc                                                 >  1:4396067/1‑150 (MQ=255)
                                                                                                 aaaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc                                                 <  1:2438357/150‑1 (MQ=255)
                                                                                                  aaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACg                                                >  1:3179282/1‑150 (MQ=255)
                                                                                                   aGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTCGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACgg                                               >  2:2879529/1‑150 (MQ=255)
                                                                                                     tGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGaa                                             >  2:5122095/1‑150 (MQ=255)
                                                                                                       gTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGCATg                                           >  2:4601309/1‑150 (MQ=255)
                                                                                                          aaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCaa                                        >  1:2033319/1‑150 (MQ=255)
                                                                                                          aaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCaa                                        >  2:4972116/1‑150 (MQ=255)
                                                                                                          aaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCaa                                        >  1:795268/1‑150 (MQ=255)
                                                                                                           aTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCaaa                                       >  1:1202642/1‑150 (MQ=255)
                                                                                                             ctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAAtt                                     >  2:3827098/1‑150 (MQ=255)
                                                                                                               cGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGc                                   >  1:5052058/1‑150 (MQ=255)
                                                                                                                 cTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCg                                 >  2:1266975/1‑150 (MQ=255)
                                                                                                                     aGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattc                             <  2:3123210/150‑1 (MQ=255)
                                                                                                                     aGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattc                             >  2:570459/1‑150 (MQ=255)
                                                                                                                        ccGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcatt                          <  1:93420/150‑1 (MQ=255)
                                                                                                                         cgcgCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcattc                         <  2:1888562/150‑1 (MQ=255)
                                                                                                                         cgcgCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATAGTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGattcattc                         <  2:4762667/150‑1 (MQ=255)
                                                                                                                          gcgcCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGAttcattca                        <  1:2410866/150‑1 (MQ=255)
                                                                                                                          gcgcCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGAttcattca                        <  1:4167247/150‑1 (MQ=255)
                                                                                                                           cgcCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATtcattcat                       >  2:1594613/1‑150 (MQ=255)
                                                                                                                            gcCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTcattcatt                      >  1:1118330/1‑150 (MQ=255)
                                                                                                                             ccAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCAttt                     <  2:4856824/150‑1 (MQ=255)
                                                                                                                             ccAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCAttt                     >  1:1551225/1‑150 (MQ=255)
                                                                                                                             ccAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCAttt                     <  2:79327/150‑1 (MQ=255)
                                                                                                                                gTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGtt                  >  1:4732409/1‑150 (MQ=255)
                                                                                                                                  aCTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAg                >  2:3431021/1‑150 (MQ=255)
                                                                                                                                   cTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGa               <  2:3362859/150‑1 (MQ=255)
                                                                                                                                        cTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAAt          >  2:464515/1‑150 (MQ=255)
                                                                                                                                        cTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAAt          >  1:107626/1‑150 (MQ=255)
                                                                                                                                         ttGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATc         <  2:1295487/150‑1 (MQ=255)
                                                                                                                                          tGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCg        <  2:1724527/150‑1 (MQ=255)
                                                                                                                                           gCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCgg       <  1:4131384/150‑1 (MQ=255)
                                                                                                                                            cTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATTTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCggg      >  2:2643778/1‑150 (MQ=255)
                                                                                                                                            cTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTAGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCggg      >  2:1375120/1‑150 (MQ=255)
                                                                                                                                             tGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGt     >  1:4874914/1‑150 (MQ=255)
                                                                                                                                              gtcgtaAGGTAATGTCCCTACAAATATTCCCCCATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATACGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGtt    <  2:2088048/150‑1 (MQ=255)
                                                                                                                                               tcgtaAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTa   <  1:2499504/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAA  >  CP009273/3238136‑3238429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑