Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,302,027 G→T 100% S86* (TCG→TAG)  nlpI ← lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,302,0270GT100.0% 1035.1 / NA 309S86* (TCG→TAG) nlpIlipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (159/150);  total (159/150)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 309 total aligned reads displayed.

TCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAA  >  CP009273/3301881‑3302173
                                                                                                                                                  |                                                                                                                                                  
tCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCACGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaa                                                                                                                                                 <  2:2668869/150‑1 (MQ=255)
 cAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaaa                                                                                                                                                >  2:4200876/1‑150 (MQ=255)
  aaGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaaaa                                                                                                                                               >  2:3956068/1‑150 (MQ=255)
   aGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAAt                                                                                                                                              <  2:4074290/150‑1 (MQ=255)
    gTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATc                                                                                                                                             <  1:3334858/150‑1 (MQ=255)
     tGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCg                                                                                                                                            <  1:1093275/150‑1 (MQ=255)
      gcgcGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGt                                                                                                                                           <  1:2945958/150‑1 (MQ=255)
       cgcgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGtt                                                                                                                                          >  2:2921407/1‑150 (MQ=255)
       cgcgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGtt                                                                                                                                          <  1:70648/150‑1 (MQ=255)
         cgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAc                                                                                                                                        <  1:1384782/150‑1 (MQ=255)
         cgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAc                                                                                                                                        <  2:3645115/150‑1 (MQ=255)
         cgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAc                                                                                                                                        <  1:418575/150‑1 (MQ=255)
          gTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAcg                                                                                                                                       >  1:41358/1‑150 (MQ=255)
              ttGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCt                                                                                                                                   <  2:1068927/150‑1 (MQ=255)
               tGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTa                                                                                                                                  <  2:1698514/150‑1 (MQ=255)
                 tAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAAt                                                                                                                                <  1:1699437/150‑1 (MQ=255)
                     ttGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGccc                                                                                                                            >  1:1487786/1‑150 (MQ=255)
                        gATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCa                                                                                                                         >  1:1650876/1‑150 (MQ=255)
                        gATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGACTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCa                                                                                                                         <  1:2541153/150‑1 (MQ=255)
                           cAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGAc                                                                                                                      >  2:4930747/1‑150 (MQ=255)
                              gCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCga                                                                                                                   <  1:761253/150‑1 (MQ=255)
                               cTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCgag                                                                                                                  <  1:1075740/150‑1 (MQ=255)
                                tCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCgaga                                                                                                                 <  2:525420/150‑1 (MQ=255)
                                 cAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGAc                                                                                                                >  1:1145412/1‑150 (MQ=255)
                                   aGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTa                                                                                                              <  1:3900629/150‑1 (MQ=255)
                                    gTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTAt                                                                                                             >  1:1566060/1‑150 (MQ=255)
                                       cAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCAt                                                                                                          >  2:1737260/1‑150 (MQ=255)
                                        aGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATa                                                                                                         >  1:1520346/1‑150 (MQ=255)
                                        aGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATa                                                                                                         >  1:4429348/1‑150 (MQ=255)
                                          taTCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTaa                                                                                                                                 >  1:2879475/2‑124 (MQ=255)
                                          taTCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTaa                                                                                                                                 <  2:286190/123‑1 (MQ=255)
                                          aaTCAAACGCTTCATAGGCAGCATCAAAAGTGCCTGCCTGCGTTAACTATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATaca                                                                                                       <  2:4813843/150‑1 (MQ=255)
                                               aaCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACt                                                                                                  <  1:3123690/150‑1 (MQ=255)
                                                 cGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTcc                                                                                                >  1:2708409/1‑150 (MQ=255)
                                                 cGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTcc                                                                                                >  2:670328/1‑150 (MQ=255)
                                                   cTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTACTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcgc                                                                                              >  2:3551517/1‑150 (MQ=255)
                                                      cATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCt                                                                                           >  2:970869/1‑150 (MQ=255)
                                                        tAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCa                                                                                         <  1:2210480/150‑1 (MQ=255)
                                                         aGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCat                                                                                        >  1:1317378/1‑150 (MQ=255)
                                                           gcagcaTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCatat                                                                                      >  2:3508753/1‑150 (MQ=255)
                                                             agcaTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaa                                                                                    >  1:1358575/1‑150 (MQ=255)
                                                               caTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaaaa                                                                                  <  1:4332284/150‑1 (MQ=255)
                                                                 tCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGc                                                                                >  2:1991610/1‑150 (MQ=255)
                                                                  cAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCt                                                                               <  2:2104946/150‑1 (MQ=255)
                                                                   aaacTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCtg                                                                              <  2:3683930/150‑1 (MQ=255)
                                                                     aaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCtgtg                                                                            <  2:5069595/150‑1 (MQ=255)
                                                                      aTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTgc                                                                           <  2:2316421/150‑1 (MQ=255)
                                                                       ttGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTgcg                                                                          >  1:2586098/1‑150 (MQ=255)
                                                                          cctgcctgCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGt                                                                       >  2:2794839/1‑150 (MQ=255)
                                                                           ctgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGtt                                                                      >  1:1803425/1‑150 (MQ=255)
                                                                           ctgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGtt                                                                      <  1:2789215/150‑1 (MQ=255)
                                                                            tgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTc                                                                     <  2:509249/150‑1 (MQ=255)
                                                                             gcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCg                                                                    <  1:908987/150‑1 (MQ=255)
                                                                             gcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCg                                                                    >  1:4788510/1‑150 (MQ=255)
                                                                             gcctgaGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCg                                                                    <  2:2746940/150‑1 (MQ=255)
                                                                                tgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCa                                                                 <  2:2440609/150‑1 (MQ=255)
                                                                                 gcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCAt                                                                >  2:2345807/1‑150 (MQ=255)
                                                                                  cGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATc                                                               <  1:4381511/150‑1 (MQ=255)
                                                                                   gTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCg                                                              <  2:219629/150‑1 (MQ=255)
                                                                                      aaaTATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGtt                                                           >  2:88323/1‑150 (MQ=255)
                                                                                         tatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTaaa                                                        <  1:3596854/150‑1 (MQ=255)
                                                                                             tGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGccc                                                    <  1:2625184/150‑1 (MQ=255)
                                                                                               ccTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGa                                                  >  2:1779224/1‑150 (MQ=255)
                                                                                               ccTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGa                                                  >  2:1403605/1‑150 (MQ=255)
                                                                                                 tAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACt                                                <  1:3174933/150‑1 (MQ=255)
                                                                                                       aTTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCTCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAg                                          <  2:1904057/150‑1 (MQ=255)
                                                                                                       aTTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAg                                          >  1:2157513/1‑150 (MQ=255)
                                                                                                        ttGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAgg                                         <  2:435443/150‑1 (MQ=255)
                                                                                                           aaTACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAtt                                      >  2:4684309/1‑150 (MQ=255)
                                                                                                            aTACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAttt                                     >  1:2081381/1‑150 (MQ=255)
                                                                                                             tACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTg                                    >  2:764670/1‑150 (MQ=255)
                                                                                                                 tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTcc                                >  2:3790739/1‑150 (MQ=255)
                                                                                                                   aGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCAt                              >  2:3427337/1‑150 (MQ=255)
                                                                                                                    ggCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATa                             <  2:2700552/150‑1 (MQ=255)
                                                                                                                    ggCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATa                             <  1:4372166/150‑1 (MQ=255)
                                                                                                                     gCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATAc                            <  2:2619113/150‑1 (MQ=255)
                                                                                                                       atatCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGt                          <  1:5030825/150‑1 (MQ=255)
                                                                                                                        tatCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTg                         <  1:839544/150‑1 (MQ=255)
                                                                                                                        tatCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTg                         >  1:485113/1‑150 (MQ=255)
                                                                                                                          tCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGcc                       >  2:2585313/1‑150 (MQ=255)
                                                                                                                           ccGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCa                      <  1:559686/150‑1 (MQ=255)
                                                                                                                           ccGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCa                      <  1:3956068/150‑1 (MQ=255)
                                                                                                                            cGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAg                     >  2:3018407/1‑150 (MQ=255)
                                                                                                                             ggTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGa                    >  2:1139837/1‑150 (MQ=255)
                                                                                                                             ggTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGa                    >  2:1327969/1‑150 (MQ=255)
                                                                                                                                cGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATc                 >  2:2355683/1‑150 (MQ=255)
                                                                                                                                 ggATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCa                >  1:1990389/1‑150 (MQ=255)
                                                                                                                                   aTTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACt              >  1:3677587/1‑150 (MQ=255)
                                                                                                                                    ttGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACtt             <  1:4557697/150‑1 (MQ=255)
                                                                                                                                      gCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTcc           >  1:4772490/1‑150 (MQ=255)
                                                                                                                                      gCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTcc           <  2:930138/150‑1 (MQ=255)
                                                                                                                                       ccAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCct          <  2:4393493/150‑1 (MQ=255)
                                                                                                                                       ccAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCct          <  1:2816510/150‑1 (MQ=255)
                                                                                                                                       ccAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCct          >  2:1350347/1‑150 (MQ=255)
                                                                                                                                       ccAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCATCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCct          <  2:3112364/150‑1 (MQ=255)
                                                                                                                                        cAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctg         >  2:2253661/1‑150 (MQ=255)
                                                                                                                                         aGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctgc        >  1:792258/1‑150 (MQ=255)
                                                                                                                                             cTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTa    >  1:3263746/1‑150 (MQ=255)
                                                                                                                                             cTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTa    >  2:3259649/1‑150 (MQ=255)
                                                                                                                                               tGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTaaa  >  1:3833128/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAA  >  CP009273/3301881‑3302173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑