Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,306,165 C→A 91.4% V125F (GTT→TTT)  rbfA ← 30s ribosome binding factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,306,1650CA91.4% 813.7 / 63.7 304V125F (GTT→TTT) rbfA30s ribosome binding factor
Reads supporting (aligned to +/- strand):  ref base C (14/12);  new base A (122/156);  total (136/168)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

Only 100 of 307 total aligned reads displayed.

CGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAG  >  CP009273/3306018‑3306311
                                                                                                                                                   |                                                                                                                                                  
 ggTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacg                                                                                                                                                 <  1:301166/150‑1 (MQ=255)
   ttGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacgac                                                                                                                                               <  1:4807671/150‑1 (MQ=255)
   ttGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacgac                                                                                                                                               <  2:3605226/150‑1 (MQ=255)
    tGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGTTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacgacg                                                                                                                                              >  1:4564879/1‑150 (MQ=255)
    tGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACacgacg                                                                                                                                              <  2:2165443/150‑1 (MQ=255)
    tGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacgacg                                                                                                                                              >  1:4312169/1‑150 (MQ=255)
    tGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacgacg                                                                                                                                              >  1:1640928/1‑150 (MQ=255)
    tGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacgacg                                                                                                                                              >  1:4907634/1‑150 (MQ=255)
    tGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacgacg                                                                                                                                              >  1:309384/1‑150 (MQ=255)
     ggCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGt                                                                                                                                             <  1:416863/150‑1 (MQ=255)
      gCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGtt                                                                                                                                            <  2:3075105/150‑1 (MQ=255)
        gTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGttct                                                                                                                                          >  1:1914346/1‑150 (MQ=255)
         ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttctt                                                                                                                                         >  1:3753878/1‑150 (MQ=255)
         ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGttctt                                                                                                                                         >  1:4727244/1‑150 (MQ=255)
          tatatataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttcttc                                                                                                                                        <  2:876402/150‑1 (MQ=255)
            tatataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGt                                                                                                                                      >  1:3807479/1‑150 (MQ=255)
                 aCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGt                                                                                                                                 <  2:3447408/150‑1 (MQ=255)
                  cGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGtt                                                                                                                                >  1:1935769/1‑150 (MQ=255)
                  cGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGtt                                                                                                                                >  2:2402294/1‑150 (MQ=255)
                   gTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGttt                                                                                                                               >  2:504975/1‑150 (MQ=255)
                   gTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGttt                                                                                                                               <  1:4950847/150‑1 (MQ=255)
                    tttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTg                                                                                                                              <  1:2563611/150‑1 (MQ=255)
                     ttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGa                                                                                                                             >  1:2522829/1‑150 (MQ=255)
                     ttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGa                                                                                                                             >  1:4795850/1‑150 (MQ=255)
                        aCTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCa                                                                                                                          <  2:2973539/150‑1 (MQ=255)
                        aCTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCa                                                                                                                          >  1:3585521/1‑150 (MQ=255)
                          tttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                        <  2:4331159/150‑1 (MQ=255)
                           ttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGc                                                                                                                       <  2:1654252/150‑1 (MQ=255)
                            tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCt                                                                                                                      <  2:1318804/150‑1 (MQ=255)
                             ttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTg                                                                                                                     <  1:428169/150‑1 (MQ=255)
                             ttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTg                                                                                                                     <  2:3271696/150‑1 (MQ=255)
                               gCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGt                                                                                                                   <  2:4080346/150‑1 (MQ=255)
                                cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTc                                                                                                                  <  1:330876/150‑1 (MQ=255)
                                   cgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAcc                                                                                                               >  2:107619/1‑150 (MQ=255)
                                   cgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGATCTTCGTCATGTTTGACCACGCTGGTCAcc                                                                                                               >  1:1891906/1‑150 (MQ=255)
                                     cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAg                                                                                                             >  1:2289307/1‑150 (MQ=255)
                                        cGTTGCTGTACATACCCTGAGGTTTATCCAGCAAAAAAACGCCGTTAATGTCGCGACCGCTACGACGAGGACGACTCATTAGTCCTACTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGtt                                                                                                          <  1:131980/150‑1 (MQ=255)
                                        cGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGtt                                                                                                          >  2:5158697/1‑150 (MQ=255)
                                           tGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGa                                                                                                       <  2:1611659/150‑1 (MQ=255)
                                           tGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGa                                                                                                       <  1:3859020/150‑1 (MQ=255)
                                           tGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGa                                                                                                       >  1:5104632/1‑150 (MQ=255)
                                             cTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACa                                                                                                     <  2:207829/150‑1 (MQ=255)
                                              tGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                                                                                                    >  1:2487264/1‑150 (MQ=255)
                                                gACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATgc                                                                                                  <  1:1973494/150‑1 (MQ=255)
                                                 aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATgcg                                                                                                 >  1:4638516/1‑150 (MQ=255)
                                                   aTACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCa                                                                                               <  2:1788526/150‑1 (MQ=255)
                                                   aTACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCa                                                                                               >  1:4021131/1‑150 (MQ=255)
                                                        cTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCt                                                                                          <  1:4400778/150‑1 (MQ=255)
                                                         tGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCtt                                                                                         >  2:542140/1‑150 (MQ=255)
                                                           aGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCa                                                                                       <  2:2638912/150‑1 (MQ=255)
                                                           aGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCa                                                                                       <  1:1442922/150‑1 (MQ=255)
                                                            ggTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCaa                                                                                      <  1:102303/150‑1 (MQ=255)
                                                             gTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAc                                                                                     <  1:3425677/150‑1 (MQ=255)
                                                              tttATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAcc                                                                                    <  1:4111885/150‑1 (MQ=255)
                                                               ttATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCa                                                                                   >  2:2606997/1‑150 (MQ=255)
                                                                  tCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagag                                                                                >  1:862223/1‑150 (MQ=255)
                                                                   ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagaga                                                                               <  1:957375/150‑1 (MQ=255)
                                                                   ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagaga                                                                               <  1:81753/150‑1 (MQ=255)
                                                                     aGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGt                                                                             >  1:923314/1‑150 (MQ=255)
                                                                      gCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGtt                                                                            >  2:4074701/1‑150 (MQ=255)
                                                                       caacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTg                                                                           >  1:2656139/1‑150 (MQ=255)
                                                                       caacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTg                                                                           <  1:1575455/150‑1 (MQ=255)
                                                                         acaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTc                                                                         >  2:663818/1‑150 (MQ=255)
                                                                           aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                       >  2:478488/1‑150 (MQ=255)
                                                                            aaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTa                                                                      >  2:4680178/1‑150 (MQ=255)
                                                                                  cGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAgaagaa                                                                >  2:1460461/1‑150 (MQ=255)
                                                                                    ttAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGaagaaga                                                              <  2:5079471/150‑2 (MQ=255)
                                                                                     tAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGt                                                             >  1:3501744/1‑150 (MQ=255)
                                                                                     tAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGt                                                             <  1:615079/150‑1 (MQ=255)
                                                                                      aaTGTCGCGACCGCGACGACGAGGACGACTCTTTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTc                                                            <  1:503147/150‑1 (MQ=255)
                                                                                       aTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCa                                                           <  1:2788482/150‑1 (MQ=255)
                                                                                            gcgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTcc                                                      <  2:4208853/150‑1 (MQ=255)
                                                                                             cgaccgcgacGACGTGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCTTGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCg                                                     <  2:4246513/150‑1 (MQ=255)
                                                                                             cgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCg                                                     >  2:3161969/1‑150 (MQ=255)
                                                                                                    gacgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGAt                                              >  1:2350192/1‑150 (MQ=255)
                                                                                                      cgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATAc                                            >  2:1019819/1‑150 (MQ=255)
                                                                                                           aGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAgg                                       <  2:1875023/150‑1 (MQ=255)
                                                                                                              acgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGgcgc                                    >  2:4647580/1‑150 (MQ=255)
                                                                                                              acgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGgcgc                                    >  2:4367787/1‑150 (MQ=255)
                                                                                                               cgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCa                                   <  2:743225/150‑1 (MQ=255)
                                                                                                               cgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCa                                   >  2:1244081/1‑150 (MQ=255)
                                                                                                                 acTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATc                                 <  1:738569/150‑1 (MQ=255)
                                                                                                                  cTCATGAGGCCTCCGCGCGGTCGGCCGGGTTACCACGACGGTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCAGCCCGGCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCg                                <  2:2942274/150‑1 (MQ=255)
                                                                                                                     aTTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCtt                             >  1:1190251/1‑150 (MQ=255)
                                                                                                                         gTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTccc                         <  2:4913652/150‑1 (MQ=255)
                                                                                                                             tcctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCcagc                     <  2:4496034/150‑1 (MQ=255)
                                                                                                                                ttGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAgg                  <  2:4727244/150‑1 (MQ=255)
                                                                                                                                 tGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGc                 <  1:1040739/150‑1 (MQ=255)
                                                                                                                                  gCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCt                >  2:116380/1‑150 (MQ=255)
                                                                                                                                    tGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGc              <  2:1694480/150‑1 (MQ=255)
                                                                                                                                     gtcgtcCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCg             <  2:3753878/150‑1 (MQ=255)
                                                                                                                                      tcgtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCgg            >  1:1274879/1‑150 (MQ=255)
                                                                                                                                        gtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGAt          <  2:2709248/150‑1 (MQ=255)
                                                                                                                                         tcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATg         <  2:2955320/150‑1 (MQ=255)
                                                                                                                                           cGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGaa       <  1:2077912/150‑1 (MQ=255)
                                                                                                                                           cGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGaa       >  2:4050084/1‑150 (MQ=255)
                                                                                                                                           cGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGaa       <  2:3807479/150‑1 (MQ=255)
                                                                                                                                             ggTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAc     <  1:4322390/150‑1 (MQ=255)
                                                                                                                                             ggTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAc     <  1:4617732/150‑1 (MQ=255)
                                                                                                                                                tAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAg  >  1:532498/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAG  >  CP009273/3306018‑3306311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑