Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,407,969 C→A 100% N249K (AAC→AAA)  acrE → cytoplasmic membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,407,9690CA98.6% 964.7 / 2.3 309N249K (AAC→AAA) acrEcytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (162/138);  total (167/142)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 309 total aligned reads displayed.

TGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATG  >  CP009273/3407822‑3408114
                                                                                                                                                   |                                                                                                                                                 
tGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAgg                                                                                                                                                 >  2:1142732/1‑150 (MQ=255)
tGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAgg                                                                                                                                                 >  2:2084577/1‑150 (MQ=255)
  aaCTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTc                                                                                                                                               <  2:3674684/150‑1 (MQ=255)
  aaCTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTc                                                                                                                                               <  2:5128409/150‑1 (MQ=255)
   aCTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCa                                                                                                                                              <  1:4663109/150‑1 (MQ=255)
        cGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCt                                                                                                                                         <  2:2372757/150‑1 (MQ=255)
          aCTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTAt                                                                                                                                       <  2:3141983/150‑1 (MQ=255)
           cTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATc                                                                                                                                      <  1:3505609/150‑1 (MQ=255)
           cTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATc                                                                                                                                      >  1:607947/1‑150 (MQ=255)
            tGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATcc                                                                                                                                     <  1:3019905/150‑1 (MQ=255)
              tCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATcccc                                                                                                                                   >  1:2988941/1‑150 (MQ=255)
               ccAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCt                                                                                                                                  <  2:2535501/150‑1 (MQ=255)
               ccAGCAGCTCGATCCGATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCt                                                                                                                                  <  2:754430/150‑1 (MQ=255)
                cagcagCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGTTTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTg                                                                                                                                 <  1:4293024/150‑1 (MQ=255)
                cagcagCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTg                                                                                                                                 <  2:4970723/150‑1 (MQ=255)
                 agcagcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGa                                                                                                                                >  1:1065824/1‑150 (MQ=255)
                     gcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAgg                                                                                                                            >  2:791541/1‑150 (MQ=255)
                       tCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTa                                                                                                                          <  2:4685661/150‑1 (MQ=255)
                       tCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTa                                                                                                                          <  2:2652186/150‑1 (MQ=255)
                            ccTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTg                                                                                                                     <  2:4645397/150‑1 (MQ=255)
                              tATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCa                                                                                                                   >  2:3092615/1‑150 (MQ=255)
                                tCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCACGGAAATTTGCATAAGGAAAACGCCACCAGCACCGTAGAGTTGGTCATGGAAAAGGGTCAAACCTATCCCCTGAAAGGTACGCTGCAAt                                                                                                                 >  2:1801816/1‑150 (MQ=255)
                                tCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAAt                                                                                                                 <  2:2421574/150‑1 (MQ=255)
                                   aCGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtct                                                                                                              <  2:2482937/150‑1 (MQ=255)
                                     gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTcc                                                                                                            <  1:1421064/150‑1 (MQ=255)
                                      ttGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCg                                                                                                           <  1:3627777/150‑1 (MQ=255)
                                      ttGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCg                                                                                                           >  2:2853409/1‑150 (MQ=255)
                                       tGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGa                                                                                                          <  2:3681002/150‑1 (MQ=255)
                                              aCCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGAcc                                                                                                   >  1:698336/1‑150 (MQ=255)
                                                ccAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGt                                                                                                 >  2:1355072/1‑150 (MQ=255)
                                                  aaTCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtg                                                                                               >  1:1346509/1‑150 (MQ=255)
                                                  aaTCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtg                                                                                               >  2:4433088/1‑150 (MQ=255)
                                                    tCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtgat                                                                                             <  1:457682/150‑1 (MQ=255)
                                                     ccAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtgatg                                                                                            >  1:4448789/1‑150 (MQ=255)
                                                         cAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAg                                                                                        <  2:3575536/150‑1 (MQ=255)
                                                          aaCGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGc                                                                                       >  1:4542908/1‑150 (MQ=255)
                                                             gATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAcc                                                                                    >  1:386677/1‑150 (MQ=255)
                                                                 ttATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCt                                                                                >  1:748361/1‑150 (MQ=255)
                                                                  tATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTc                                                                               <  2:3999928/150‑1 (MQ=255)
                                                                  tATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTc                                                                               <  1:4065534/150‑1 (MQ=255)
                                                                  tATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCCCCGATGTGACCGTTGATGAAAGCACCGGCTc                                                                               >  2:414839/1‑150 (MQ=255)
                                                                   aTGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTcc                                                                              >  1:2253275/1‑150 (MQ=255)
                                                                     gAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCAt                                                                            <  1:4968320/150‑1 (MQ=255)
                                                                      aGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATa                                                                           <  2:3959061/150‑1 (MQ=255)
                                                                          tGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAccc                                                                       <  2:900898/150‑1 (MQ=255)
                                                                          tGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAccc                                                                       >  2:3595765/1‑150 (MQ=255)
                                                                           gAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCt                                                                      >  1:1781646/1‑150 (MQ=255)
                                                                            aaGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTa                                                                     >  1:21095/1‑150 (MQ=255)
                                                                            aaGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCATTGATGAAAGCACCGGCTCCATAACCCTa                                                                     >  2:4942947/1‑150 (MQ=255)
                                                                             aGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTAc                                                                    >  1:3671030/1‑150 (MQ=255)
                                                                              gCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACg                                                                   <  1:1718108/150‑1 (MQ=255)
                                                                                aaTCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTg                                                                 >  1:994628/1‑150 (MQ=255)
                                                                                     gTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTc                                                            >  2:4793504/1‑150 (MQ=255)
                                                                                      tAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCt                                                           >  1:3211090/1‑150 (MQ=255)
                                                                                       agagCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCtt                                                          >  2:5025541/1‑150 (MQ=255)
                                                                                         agCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTcc                                                        >  1:1903216/1‑150 (MQ=255)
                                                                                         agCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTcc                                                        <  2:1641214/150‑1 (MQ=255)
                                                                                            aaGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTa                                                     >  1:291325/1‑150 (MQ=255)
                                                                                                aaaTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAAccc                                                 >  2:3412950/1‑150 (MQ=255)
                                                                                                   tttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCa                                              <  2:2251555/150‑1 (MQ=255)
                                                                                                       cATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAt                                          <  1:1519496/150‑1 (MQ=255)
                                                                                                        aTAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATa                                         <  1:663807/150‑1 (MQ=255)
                                                                                                        aTAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATa                                         >  2:4154078/1‑150 (MQ=255)
                                                                                                          aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACg                                       >  2:2720604/1‑150 (MQ=255)
                                                                                                          aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACg                                       >  1:3598943/1‑150 (MQ=255)
                                                                                                          aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACg                                       >  1:5035372/1‑150 (MQ=255)
                                                                                                           aGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGc                                      >  2:571424/1‑150 (MQ=255)
                                                                                                            ggAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCt                                     >  2:1248509/1‑150 (MQ=255)
                                                                                                               aaaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCtttt                                  <  1:485838/150‑1 (MQ=255)
                                                                                                                aaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTg                                 >  2:3793561/1‑150 (MQ=255)
                                                                                                                  cGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGcc                               <  2:4572419/150‑1 (MQ=255)
                                                                                                                   gCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCg                              <  1:2510611/150‑1 (MQ=255)
                                                                                                                    ccaccaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCgg                             <  2:1723781/150‑1 (MQ=255)
                                                                                                                     caccaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCggg                            >  1:4896797/1‑150 (MQ=255)
                                                                                                                     caccaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCGTTTGTCggg                            >  1:4120578/1‑150 (MQ=255)
                                                                                                                      accaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGt                           >  1:3311314/1‑150 (MQ=255)
                                                                                                                        caGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTAt                         >  1:2804463/1‑150 (MQ=255)
                                                                                                                         atcAAAGTATAGTTGATCATTGAAAAAGGTCAAACCAATCCCATGAAAGGAACGATGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACTTGCTGTCTTCCCTAACCCGCAACATACTCTTTACCCGGGTCTg                        >  2:158992/3‑150 (MQ=255)
                                                                                                                         aGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATg                        <  1:3765233/150‑1 (MQ=255)
                                                                                                                         aGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATg                        >  2:158875/1‑150 (MQ=255)
                                                                                                                             aCGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTtg                    <  1:1966763/150‑1 (MQ=255)
                                                                                                                             aCGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTtg                    <  2:882834/150‑1 (MQ=255)
                                                                                                                             aCGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTtg                    <  1:823654/150‑1 (MQ=255)
                                                                                                                               gTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTtgtg                  <  2:4199328/150‑1 (MQ=255)
                                                                                                                                  gagTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcgt               >  1:3050545/1‑150 (MQ=255)
                                                                                                                                  gagTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcgt               <  2:2918334/150‑1 (MQ=255)
                                                                                                                                   agTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATATGCTTTTGCCGGGTATGTTTgtgcgtg              >  1:2599647/1‑150 (MQ=255)
                                                                                                                                    gTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcgtgc             <  2:4359433/150‑1 (MQ=255)
                                                                                                                                    gTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcgtgc             <  2:1223991/150‑1 (MQ=255)
                                                                                                                                     ttGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCa            >  2:1680356/1‑150 (MQ=255)
                                                                                                                                     ttGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCa            >  2:3271462/1‑150 (MQ=255)
                                                                                                                                      tGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCAc           >  1:256587/1‑150 (MQ=255)
                                                                                                                                      tGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCAc           <  2:1119843/150‑1 (MQ=255)
                                                                                                                                      tGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCAc           >  1:1613715/1‑150 (MQ=255)
                                                                                                                                      tGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCAc           <  1:3952451/150‑1 (MQ=255)
                                                                                                                                       ggTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACg          <  2:655412/150‑1 (MQ=255)
                                                                                                                                           attgAAAATGGTCAAACCTATCCCATTAAAGGTACGCTGCAATTCTCCGATTTGACCTTTGATGAAAGCAGCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCTCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGAtt      <  1:2241894/147‑1 (MQ=255)
                                                                                                                                           aTGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGAtt      >  1:5087289/1‑150 (MQ=255)
                                                                                                                                             ggAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtga    <  2:737621/150‑1 (MQ=255)
                                                                                                                                               aaaaAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtgatg  >  2:3063411/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATG  >  CP009273/3407822‑3408114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑