Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,832 T→C 100% N99D (AAC→GAC)  rplX ← 50S ribosomal subunit protein L24

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,8320TC99.7% 1126.9 / ‑5.0 291N99D (AAC→GAC) rplX50S ribosomal subunit protein L24
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (139/151);  total (139/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 291 total aligned reads displayed.

ATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  >  CP009273/3440685‑3440978
                                                                                                                                                   |                                                                                                                                                  
   ttCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACt                                                                                                                                               <  2:3923063/150‑1 (MQ=255)
    tCAGGGTGATCTTCTCGACCCGAGGGACTTGCATTACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACtt                                                                                                                                              <  2:1141695/150‑1 (MQ=255)
     cAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTg                                                                                                                                             <  2:1576127/150‑1 (MQ=255)
        ggTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaag                                                                                                                                          >  2:2701548/1‑150 (MQ=255)
            aTCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAAc                                                                                                                                      >  1:3298223/1‑150 (MQ=255)
             tcttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACg                                                                                                                                     <  2:427831/150‑1 (MQ=255)
             tcttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACg                                                                                                                                     >  1:499777/1‑150 (MQ=255)
              cttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACgg                                                                                                                                    >  1:3943389/1‑150 (MQ=255)
                tctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGAc                                                                                                                                  >  2:190600/1‑150 (MQ=255)
                 ctcgcaccGAGGCACTGGCCTGACCGCATGGTCGTGAAACTCAGTCCTGAGTTTTGGAACTACTTCGTCTTGGTTGTACTCAGGCAGTTTCGCCATCGTACTACTCCATCTTACGTGATAGTTTCGCGGTCAGACTTGAAGAAACGGACt                                                                                                                                 <  2:4832861/144‑1 (MQ=255)
                 ctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAATAAACTGACt                                                                                                                                 >  1:2683824/1‑150 (MQ=255)
                 ctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACt                                                                                                                                 >  2:5054345/1‑150 (MQ=255)
                 ctcGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACt                                                                                                                                 >  1:1514533/1‑150 (MQ=255)
                    gACCCGATGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttt                                                                                                                              <  2:1393491/150‑1 (MQ=255)
                     aCCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACttttt                                                                                                                             <  2:4174744/150‑1 (MQ=255)
                      cccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttttt                                                                                                                            <  1:3914530/150‑1 (MQ=255)
                      cccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttttt                                                                                                                            <  1:3710764/150‑1 (MQ=255)
                      cccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttttt                                                                                                                            >  2:1030517/1‑150 (MQ=255)
                        cGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAc                                                                                                                          >  1:1933606/1‑150 (MQ=255)
                         gAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAcc                                                                                                                         >  2:3164442/1‑150 (MQ=255)
                         gAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAcc                                                                                                                         <  1:3687211/150‑1 (MQ=255)
                         gAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAcc                                                                                                                         <  1:3445192/150‑1 (MQ=255)
                         gAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTAcc                                                                                                                         >  1:4511098/1‑150 (MQ=255)
                             gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                                                                                     <  1:4652624/150‑1 (MQ=255)
                             gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                                                                                     >  2:3152289/1‑150 (MQ=255)
                             gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                                                                                     <  2:1313680/150‑1 (MQ=255)
                             gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                                                                                     >  1:2460577/1‑150 (MQ=255)
                             gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                                                                                     >  2:243444/1‑150 (MQ=255)
                               cTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTc                                                                                                                   >  2:2141313/1‑150 (MQ=255)
                                 tGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGa                                                                                                                 >  1:580866/1‑150 (MQ=255)
                                     tGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCt                                                                                                             <  1:4816689/150‑1 (MQ=255)
                                        cAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaaa                                                                                                          >  2:339080/1‑150 (MQ=255)
                                        cAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaaa                                                                                                          >  2:886234/1‑150 (MQ=255)
                                         aGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                         >  1:909085/1‑150 (MQ=255)
                                         aGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                         <  2:3461545/150‑1 (MQ=255)
                                         aGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                         >  2:2215067/1‑150 (MQ=255)
                                                 aGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACg                                                                                                 <  2:2519827/150‑1 (MQ=255)
                                                   ttAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGt                                                                                               <  1:1611671/150‑1 (MQ=255)
                                                    tAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTc                                                                                              >  1:3778197/1‑150 (MQ=255)
                                                     aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCa                                                                                             >  1:2228720/1‑150 (MQ=255)
                                                     aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCa                                                                                             <  1:3693436/150‑1 (MQ=255)
                                                       aCTCAGTCATGTGTTTTTTAACTACTTAGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGc                                                                                           <  1:307/150‑1 (MQ=255)
                                                          cattCATGTGTTTTTTAACTTCTTCTTCTTTGTAGTAATCATGCAGTTTAGCCATCGTTCTACTCCTAATTACTTGATTTTTTCTCTGTCAGACTTTAAGAAACGGACTTTTTTACCGTCTTAGAATCTAAAGCCTACACGGTCAGCCtt                                                                                        <  1:3276639/147‑1 (MQ=255)
                                                          cAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCtt                                                                                        <  2:5123224/150‑1 (MQ=255)
                                                          cAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCtt                                                                                        <  2:2718397/150‑1 (MQ=255)
                                                           aGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                       >  1:2278720/1‑150 (MQ=255)
                                                            gTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGc                                                                                      >  2:2750689/1‑150 (MQ=255)
                                                             tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGcc                                                                                     >  2:3419360/1‑150 (MQ=255)
                                                                     tttttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                                                                             <  2:2075517/150‑1 (MQ=255)
                                                                     tttttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                                                                             <  1:4925694/150‑1 (MQ=255)
                                                                         tAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCAtt                                                                         <  1:1383951/150‑1 (MQ=255)
                                                                         tAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTGACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCAtt                                                                         <  2:2959447/150‑1 (MQ=255)
                                                                            ctactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGaa                                                                      <  1:1273015/150‑1 (MQ=255)
                                                                            ctactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGaa                                                                      >  1:4825559/1‑150 (MQ=255)
                                                                             tactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAg                                                                     >  2:3519402/1‑150 (MQ=255)
                                                                              actTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGa                                                                    >  1:762794/1‑150 (MQ=255)
                                                                                ttCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAtt                                                                  <  1:209444/150‑1 (MQ=255)
                                                                                 tCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTg                                                                 <  2:3823168/150‑1 (MQ=255)
                                                                                   gTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCt                                                               <  1:4067820/150‑1 (MQ=255)
                                                                                   gTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCt                                                               <  1:4604068/150‑1 (MQ=255)
                                                                                      tttGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACg                                                            <  2:4982410/150‑1 (MQ=255)
                                                                                          tagtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTgg                                                        >  1:233054/1‑150 (MQ=255)
                                                                                          tagtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTgg                                                        >  1:3110859/1‑150 (MQ=255)
                                                                                             taATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGaaa                                                     >  1:1230697/1‑150 (MQ=255)
                                                                                             taATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGaaa                                                     >  2:167135/1‑150 (MQ=255)
                                                                                              aaTCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAc                                                    >  2:2065249/1‑150 (MQ=255)
                                                                                                 cATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTg                                                 >  2:2610453/1‑150 (MQ=255)
                                                                                                  aTGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGa                                                >  1:1838729/1‑150 (MQ=255)
                                                                                                  aTGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGa                                                >  2:3996131/1‑150 (MQ=255)
                                                                                                    gCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAAt                                              <  1:2750689/150‑1 (MQ=255)
                                                                                                     cAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATa                                             <  1:729409/150‑1 (MQ=255)
                                                                                                      aGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAg                                            >  1:2914352/1‑150 (MQ=255)
                                                                                                          tCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCgtc                                        <  1:4220919/150‑3 (MQ=255)
                                                                                                               aTCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGcttctt                                   <  1:5054345/150‑1 (MQ=255)
                                                                                                                tCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCttt                                  >  2:2372391/1‑150 (MQ=255)
                                                                                                                tCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCttt                                  >  1:2202920/1‑150 (MQ=255)
                                                                                                                tCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCttt                                  >  1:837510/1‑150 (MQ=255)
                                                                                                                 cGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCtttt                                 >  1:3048487/1‑150 (MQ=255)
                                                                                                                   tactacTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTc                               <  1:4293392/150‑1 (MQ=255)
                                                                                                                    actactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCa                              >  1:1878904/1‑150 (MQ=255)
                                                                                                                      tactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAAc                            >  2:4657550/1‑150 (MQ=255)
                                                                                                                      tactCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAAc                            >  2:110360/1‑150 (MQ=255)
                                                                                                                       actCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACg                           <  2:1826486/150‑1 (MQ=255)
                                                                                                                           cAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGc                       <  1:1502257/150‑1 (MQ=255)
                                                                                                                            aaaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGACACCTGACTTGCGGCTTCTTTGTCGAGGATGcc                      >  2:2684668/1‑150 (MQ=255)
                                                                                                                            aaaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGcc                      <  1:5041019/150‑1 (MQ=255)
                                                                                                                            aaaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGcc                      <  1:888473/150‑1 (MQ=255)
                                                                                                                             aaTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCa                     >  1:4170771/1‑150 (MQ=255)
                                                                                                                              aTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAc                    <  1:1593613/150‑1 (MQ=255)
                                                                                                                                   ttGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggt               <  2:2789323/150‑1 (MQ=255)
                                                                                                                                       tAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttggt           >  2:2010808/1‑150 (MQ=255)
                                                                                                                                       tAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttggt           >  2:4532233/1‑150 (MQ=255)
                                                                                                                                         gTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTc         >  1:317229/1‑150 (MQ=255)
                                                                                                                                            tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      <  1:2141311/150‑1 (MQ=255)
                                                                                                                                            tCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAgg      <  1:4709619/150‑1 (MQ=255)
                                                                                                                                             cGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAggg     <  1:1010367/150‑1 (MQ=255)
                                                                                                                                              gCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGc    >  2:1746905/1‑150 (MQ=255)
                                                                                                                                              gCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGc    >  1:2826192/1‑150 (MQ=255)
                                                                                                                                              gCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGc    <  1:4404464/150‑1 (MQ=255)
                                                                                                                                               cTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGcc   <  2:4364396/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGGCCG  >  CP009273/3440685‑3440978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑