Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,445,410 C→A 100% R79L (CGT→CTT)  rplD ← 50S ribosomal subunit protein L4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,445,4100CA97.0% 909.1 / 12.6 291R79L (CGT→CTT) rplD50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base C (8/2);  new base A (149/132);  total (157/134)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 292 total aligned reads displayed.

GCGCTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACC  >  CP009273/3445261‑3445556
                                                                                                                                                     |                                                                                                                                                  
  gcTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGc                                                                                                                                                  >  1:4561925/1‑150 (MQ=255)
       tACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGAt                                                                                                                                             <  2:4592190/150‑1 (MQ=255)
         cAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCg                                                                                                                                           <  2:3975541/150‑1 (MQ=255)
          agagAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCTCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGTTCACGCCACCAGAAAGCCAGATCgg                                                                                                                                          >  2:4373771/1‑150 (MQ=255)
             gaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGct                                                                                                                                       >  1:4504847/1‑150 (MQ=255)
             gaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGct                                                                                                                                       >  2:3178636/1‑150 (MQ=255)
              aaCTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGctc                                                                                                                                      >  1:4105380/1‑150 (MQ=255)
                 ttCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTg                                                                                                                                   <  2:2478396/150‑1 (MQ=255)
                  tctcGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGTTGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGa                                                                                                                                  <  1:3037079/150‑1 (MQ=255)
                  tctcGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGa                                                                                                                                  >  1:160962/1‑150 (MQ=255)
                     cGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAg                                                                                                                               <  1:4802147/150‑1 (MQ=255)
                     cGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAg                                                                                                                               >  2:2724169/1‑150 (MQ=255)
                         aaCGATCAGACGATCCTGACGTACCAGTTCTGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAcc                                                                                                                           <  2:1263421/150‑1 (MQ=255)
                          aCGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCa                                                                                                                          <  2:3115693/150‑1 (MQ=255)
                           cGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAg                                                                                                                         <  2:670565/150‑1 (MQ=255)
                            gATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGa                                                                                                                        <  2:4843013/150‑1 (MQ=255)
                            gATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGa                                                                                                                        >  1:3327943/1‑150 (MQ=255)
                               cAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcg                                                                                                                     <  2:2632592/150‑1 (MQ=255)
                               cAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcg                                                                                                                     >  1:3567410/1‑150 (MQ=255)
                                 gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgcg                                                                                                                   >  1:2073150/1‑150 (MQ=255)
                                   cGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCa                                                                                                                 <  2:4683948/150‑1 (MQ=255)
                                    gATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTCTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                                >  1:295296/1‑150 (MQ=255)
                                     aTCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACg                                                                                                               <  1:3982858/150‑1 (MQ=255)
                                     aTCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACg                                                                                                               >  1:1387410/1‑150 (MQ=255)
                                        cTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGcc                                                                                                            >  2:4877394/1‑150 (MQ=255)
                                        cTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGcc                                                                                                            >  2:5062956/1‑150 (MQ=255)
                                          gACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCgg                                                                                                          <  2:2263519/150‑1 (MQ=255)
                                          gACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCgg                                                                                                          <  2:290251/150‑1 (MQ=255)
                                              tACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                                                                      >  2:1408884/1‑150 (MQ=255)
                                                ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCta                                                                                                    <  1:1478456/150‑2 (MQ=255)
                                                ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCtt                                                                                                    <  1:1792369/150‑1 (MQ=255)
                                                  aGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTc                                                                                                  >  1:1012501/1‑150 (MQ=255)
                                                    ttCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTg                                                                                                <  1:1284925/150‑1 (MQ=255)
                                                       ggACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcg                                                                                             >  2:2915200/1‑150 (MQ=255)
                                                       ggACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcg                                                                                             <  2:1104466/150‑1 (MQ=255)
                                                       ggACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcg                                                                                             <  1:911124/150‑1 (MQ=255)
                                                         aCAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                                                                           <  1:2422313/150‑1 (MQ=255)
                                                         aCAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                                                                           >  1:4182336/1‑150 (MQ=255)
                                                          cAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCa                                                                                          >  2:1615407/1‑150 (MQ=255)
                                                          cAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCa                                                                                          >  2:4061238/1‑150 (MQ=255)
                                                          cAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCa                                                                                          <  1:3775494/150‑1 (MQ=255)
                                                          cAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCa                                                                                          <  2:628252/150‑1 (MQ=255)
                                                           aGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                                                                         >  1:196072/1‑150 (MQ=255)
                                                           aGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                                                                         <  1:605560/150‑1 (MQ=255)
                                                            ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCATGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACg                                                                                        <  1:3489019/150‑1 (MQ=255)
                                                              aTGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGt                                                                                      >  1:3245112/1‑150 (MQ=255)
                                                                gCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                                                                    >  1:1042030/1‑150 (MQ=255)
                                                                gCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                                                                    <  1:4254157/150‑1 (MQ=255)
                                                                 cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                                                                   >  2:3724171/1‑150 (MQ=255)
                                                                 cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGAATGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATATAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                                                                   <  1:2295180/150‑1 (MQ=255)
                                                                     tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAc                                                                               <  1:1691622/150‑1 (MQ=255)
                                                                      cAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAcc                                                                              >  2:2817901/1‑150 (MQ=255)
                                                                      cAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAcc                                                                              <  2:4196285/150‑1 (MQ=255)
                                                                        gcgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCgg                                                                            <  1:2120271/150‑1 (MQ=255)
                                                                         cgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGa                                                                           >  2:3988501/1‑150 (MQ=255)
                                                                         cgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGa                                                                           <  2:1956038/150‑1 (MQ=255)
                                                                           cgccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAc                                                                         >  1:2047229/1‑150 (MQ=255)
                                                                            gccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCACAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                                                                        <  1:2218033/150‑1 (MQ=255)
                                                                              cgcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                                                                      >  1:4649673/1‑150 (MQ=255)
                                                                              cgcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                                                                      >  1:3043855/1‑150 (MQ=255)
                                                                               gcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAACAAAGGTCACGCCACCAGAAAGCCAGATAGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGt                                                                     >  1:1855212/1‑150 (MQ=255)
                                                                                  gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                                                                  >  1:2833308/1‑150 (MQ=255)
                                                                                     cATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCA‑CAGCACGCCCGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCGTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                                                              >  2:1452652/1‑150 (MQ=255)
                                                                                      aTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                                                              >  1:2571046/1‑150 (MQ=255)
                                                                                              gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGt                                                      >  2:1828141/1‑150 (MQ=255)
                                                                                                tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtct                                                    <  2:4222617/150‑1 (MQ=255)
                                                                                                  aCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc                                                  <  2:3480884/150‑1 (MQ=255)
                                                                                                   cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttct                                                 <  1:6635/150‑1 (MQ=255)
                                                                                                   cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttct                                                 <  1:3304155/150‑1 (MQ=255)
                                                                                                          aCTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACg                                          <  2:2319668/150‑1 (MQ=255)
                                                                                                           cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGa                                         >  1:5123801/1‑150 (MQ=255)
                                                                                                           cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGa                                         >  2:1427842/1‑150 (MQ=255)
                                                                                                             gtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGt                                       >  2:4256974/1‑150 (MQ=255)
                                                                                                               ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTAc                                     >  2:221336/1‑150 (MQ=255)
                                                                                                                 tCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACAAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTAccc                                   <  1:1161533/150‑1 (MQ=255)
                                                                                                                   cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGCCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTg                                 <  1:3165551/150‑1 (MQ=255)
                                                                                                                    tGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGa                                >  1:1921320/1‑150 (MQ=255)
                                                                                                                     gCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGAc                               <  2:2073150/150‑1 (MQ=255)
                                                                                                                      cGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACg                              >  2:4139447/1‑150 (MQ=255)
                                                                                                                       ggACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGa                             <  1:298935/150‑1 (MQ=255)
                                                                                                                       ggACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGa                             >  2:4517092/1‑150 (MQ=255)
                                                                                                                       ggACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGa                             >  1:3676224/1‑150 (MQ=255)
                                                                                                                        gACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAg                            >  2:5132054/1‑150 (MQ=255)
                                                                                                                         aCGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGc                           >  2:1706896/1‑150 (MQ=255)
                                                                                                                           gAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCAc                         >  2:900367/1‑150 (MQ=255)
                                                                                                                               agcaAAGGTCACGCCACCAGACAGCCAGATCGGGCTCTTGATAGAACGAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTTCCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGc                     <  2:3028561/150‑1 (MQ=255)
                                                                                                                               agcaAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGc                     <  2:2368623/150‑1 (MQ=255)
                                                                                                                                   aaGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCa                 >  2:4239247/1‑150 (MQ=255)
                                                                                                                                   aaGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCa                 <  2:3327943/150‑1 (MQ=255)
                                                                                                                                    aGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCAt                <  2:3431971/150‑1 (MQ=255)
                                                                                                                                    aGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCAt                >  1:34266/1‑150 (MQ=255)
                                                                                                                                     ggTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATa               <  2:3007867/150‑1 (MQ=255)
                                                                                                                                        cACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGc            >  2:1248257/1‑150 (MQ=255)
                                                                                                                                          cGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaa          >  1:3589995/1‑150 (MQ=255)
                                                                                                                                          cGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaa          <  1:2807668/150‑1 (MQ=255)
                                                                                                                                           gCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaac         >  2:2189951/1‑150 (MQ=255)
                                                                                                                                            ccaccaGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaaca        <  2:3689006/150‑1 (MQ=255)
                                                                                                                                            ccaccaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaaca        >  2:2015047/1‑150 (MQ=255)
                                                                                                                                            ccaccaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaaca        >  1:2733698/1‑150 (MQ=255)
                                                                                                                                              accaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaac      <  2:1171571/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCGCTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACC  >  CP009273/3445261‑3445556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑