Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,455,867 C→A 100% I55I (ATC→ATA)  gspJ → putative general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,455,8670CA99.9% 945.0 / ‑7.0 286I55I (ATC→ATA) gspJputative general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (152/133);  total (153/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 292 total aligned reads displayed.

GTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCAC  >  CP009273/3455722‑3456013
                                                                                                                                                 |                                                                                                                                                  
   tCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTc                                                                                                                                             >  1:2982518/1‑150 (MQ=255)
    cacaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCt                                                                                                                                            >  2:3918025/1‑150 (MQ=255)
      caTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTg                                                                                                                                          <  2:1826309/150‑1 (MQ=255)
      caTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTg                                                                                                                                          >  2:3348353/1‑150 (MQ=255)
      caTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTg                                                                                                                                          <  2:1170820/150‑1 (MQ=255)
       aTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGa                                                                                                                                         >  2:2415112/1‑150 (MQ=255)
       aTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGa                                                                                                                                         >  2:3820875/1‑150 (MQ=255)
        ttGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGAt                                                                                                                                        <  1:970726/150‑1 (MQ=255)
        ttGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGAt                                                                                                                                        <  2:658106/150‑1 (MQ=255)
          gCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATTATTTTTTAGCAAGCTTCCGAGTTGCATCAACGCACACAGAAAGAAAACCAGCAGTTCAATCATCTACAGCGCAACATAACCATTATTGATaa                                                                                                                                      >  1:4712420/1‑150 (MQ=255)
           cTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAAc                                                                                                                                     >  1:3237248/1‑150 (MQ=255)
                ggTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGAtct                                                                                                                                >  1:854365/1‑150 (MQ=255)
                     tGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGc                                                                                                                           <  2:5092007/150‑1 (MQ=255)
                     tGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGc                                                                                                                           <  2:1085008/150‑1 (MQ=255)
                     tGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAAACCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGc                                                                                                                           >  1:2470619/1‑150 (MQ=255)
                      ggcggcGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCa                                                                                                                          <  1:3068327/150‑1 (MQ=255)
                             cTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTc                                                                                                                   >  2:1121584/1‑150 (MQ=255)
                              tGGCGATTTTCTCTATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATAAACGCTCACAGAACGAATTACAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCg                                                                                                                  <  1:3441859/150‑1 (MQ=255)
                              tGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCg                                                                                                                  >  2:28987/1‑150 (MQ=255)
                              tGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCg                                                                                                                  <  2:4078454/150‑1 (MQ=255)
                               ggCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGc                                                                                                                 >  2:3693165/1‑150 (MQ=255)
                                gCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGcc                                                                                                                <  1:4330836/150‑1 (MQ=255)
                                gCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGcc                                                                                                                >  2:2794999/1‑150 (MQ=255)
                                 cGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGccc                                                                                                               >  2:4531849/1‑150 (MQ=255)
                                   aTTTTCTCAATGCTTATCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCgg                                                                                                             <  1:2820793/150‑1 (MQ=255)
                                   aTTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCgg                                                                                                             >  2:4668980/1‑150 (MQ=255)
                                     tttCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCggag                                                                                                           <  1:270834/150‑1 (MQ=255)
                                        ctcAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGaa                                                                                                        >  1:1616991/1‑150 (MQ=255)
                                            aTGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCgg                                                                                                    >  1:1113142/1‑150 (MQ=255)
                                            aTGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTGCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCgg                                                                                                    >  1:3814641/1‑150 (MQ=255)
                                             tGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGa                                                                                                   <  1:606187/150‑1 (MQ=255)
                                             tGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGa                                                                                                   >  2:22934/1‑150 (MQ=255)
                                             tGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTAGCCCGGAGGAATCGGa                                                                                                   >  2:3521337/1‑150 (MQ=255)
                                               cTTAGCGTGCTGGCGATTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGt                                                                                                 <  1:1265415/150‑1 (MQ=255)
                                                ttAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATACAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATATCCTGCAACTGGTCGCCCGGAGGAATCGGAGTa                                                                                                >  1:595056/1‑150 (MQ=255)
                                                  aGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAAAAAGCTACAGCGCACCAAAACCATTCTTGATAACGATCTCCTGCAACAGGTCGCCCGGAGGAATCGGAGTACg                                                                                              >  1:5108964/1‑150 (MQ=255)
                                                  aGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTAAATCAGCTACAGCGCACCAAAACCATTCTTTATAACGATCTACTGCAACTGGTCGCCCGGAGGAATCGGAGTACg                                                                                              >  1:4050484/1‑150 (MQ=255)
                                                    cGTGCTGGCGTTTATGATTTTTTCGCAAGCTTACGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTAAATCAGCTAAAGCGCACCATAAACAATATTGATAACCATCTCCAGCAACTGGTCGCCCGGAGGAATCGGAGTACGGa                                                                                            >  1:4813028/1‑150 (MQ=255)
                                                       gCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaa                                                                                         <  1:3526342/150‑1 (MQ=255)
                                                        cTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGACAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaa                                                                                        <  2:2838619/150‑1 (MQ=255)
                                                        cTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaa                                                                                        >  2:3049079/1‑150 (MQ=255)
                                                         tGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                       >  2:1985383/1‑150 (MQ=255)
                                                         tGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTACAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                       >  2:2184809/1‑150 (MQ=255)
                                                           gCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAAta                                                                                     <  2:889081/150‑2 (MQ=255)
                                                            cGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAAtaa                                                                                    >  1:3326061/1‑148 (MQ=255)
                                                                     ttttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTgg                                                                           <  2:4164768/150‑1 (MQ=255)
                                                                      tttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTggg                                                                          <  2:954405/150‑1 (MQ=255)
                                                                        tttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTg                                                                        >  1:4986454/1‑150 (MQ=255)
                                                                         ttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTga                                                                       <  2:4973805/150‑1 (MQ=255)
                                                                            gCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaaga                                                                    <  2:4747025/150‑1 (MQ=255)
                                                                             cAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaagaa                                                                   >  2:720130/1‑150 (MQ=255)
                                                                             cAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTgaagaa                                                                   <  2:53379/150‑1 (MQ=255)
                                                                               aGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGGGTTGGGTGAAGAAGc                                                                 >  1:1040068/1‑150 (MQ=255)
                                                                                 cTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTa                                                               >  1:4844831/1‑150 (MQ=255)
                                                                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAt                                                              >  1:3575006/1‑150 (MQ=255)
                                                                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAt                                                              >  1:2561182/1‑150 (MQ=255)
                                                                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAt                                                              >  1:5116659/1‑150 (MQ=255)
                                                                                     cGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtttt                                                           >  1:3365624/1‑150 (MQ=255)
                                                                                     cGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtttt                                                           <  2:255665/150‑1 (MQ=255)
                                                                                     cGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCAGTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTAtttt                                                           <  2:3630707/150‑1 (MQ=255)
                                                                                       aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTa                                                         >  1:4648403/1‑150 (MQ=255)
                                                                                       aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTa                                                         >  1:755487/1‑150 (MQ=255)
                                                                                       aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTa                                                         >  1:3195004/1‑150 (MQ=255)
                                                                                       aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGAAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGTGTGAAGAAGCTATATTTa                                                         >  1:5155363/1‑150 (MQ=255)
                                                                                        gTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTAc                                                        <  1:1497845/150‑1 (MQ=255)
                                                                                         ttGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTAcc                                                       >  2:1465572/1‑150 (MQ=255)
                                                                                           gCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCAc                                                     >  1:173809/1‑150 (MQ=255)
                                                                                            cATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACg                                                    <  1:1077059/150‑1 (MQ=255)
                                                                                            cATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACg                                                    <  2:2131347/150‑1 (MQ=255)
                                                                                             aTCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGc                                                   >  1:2154436/1‑150 (MQ=255)
                                                                                                aaCGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCaaa                                                >  2:4812605/1‑150 (MQ=255)
                                                                                                  cGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGt                                              <  1:3285108/150‑1 (MQ=255)
                                                                                                      cacaGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTcgcg                                          <  2:1668038/150‑1 (MQ=255)
                                                                                                       acaGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAACATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGa                                         <  1:2003408/150‑1 (MQ=255)
                                                                                                        caGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGAt                                        <  2:3457709/150‑1 (MQ=255)
                                                                                                         agaaagaaATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATc                                       >  2:4525868/1‑150 (MQ=255)
                                                                                                              gaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTg                                  <  1:22934/150‑1 (MQ=255)
                                                                                                              gaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTg                                  >  1:1819424/1‑150 (MQ=255)
                                                                                                                    cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGACTCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcct                            >  1:4495375/1‑150 (MQ=255)
                                                                                                                    cagcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcct                            >  1:365182/1‑150 (MQ=255)
                                                                                                                     agcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctcctc                           <  1:19049/150‑1 (MQ=255)
                                                                                                                      gcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCctct                          <  1:1399127/150‑1 (MQ=255)
                                                                                                                      gcagTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCctct                          <  1:3977799/150‑1 (MQ=255)
                                                                                                                        agTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCa                        <  1:5158788/150‑1 (MQ=255)
                                                                                                                           tCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTg                     <  2:4426918/150‑1 (MQ=255)
                                                                                                                             aaTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGaa                   <  2:1683569/150‑1 (MQ=255)
                                                                                                                              aTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAg                  <  2:2498276/150‑1 (MQ=255)
                                                                                                                              aTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAg                  >  1:3882556/1‑150 (MQ=255)
                                                                                                                                cAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGca                >  1:4306003/1‑150 (MQ=255)
                                                                                                                                 aGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac               >  1:2406204/1‑150 (MQ=255)
                                                                                                                                 aGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac               >  1:4907111/1‑150 (MQ=255)
                                                                                                                                    tACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACaaa            >  2:5064750/1‑150 (MQ=255)
                                                                                                                                     aCAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAAc           <  1:1220232/150‑1 (MQ=255)
                                                                                                                                       aGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCt         >  1:2132713/1‑150 (MQ=255)
                                                                                                                                         cgcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTa       >  2:1310437/1‑150 (MQ=255)
                                                                                                                                          gcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTAc      <  1:4274821/150‑1 (MQ=255)
                                                                                                                                          gcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTAc      >  2:1612024/1‑150 (MQ=255)
                                                                                                                                          gcACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTAc      >  1:414047/1‑150 (MQ=255)
                                                                                                                                           caccataaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACt     <  2:1234325/150‑1 (MQ=255)
                                                                                                                                           caccataaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATAATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACt     <  2:3244662/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCAC  >  CP009273/3455722‑3456013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑