Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,535,370 C→A 100% S535R (AGC→AGA)  feoB → fused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,535,3700CA100.0% 857.1 / ‑6.7 294S535R (AGC→AGA) feoBfused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (3/1);  major base A (145/133);  minor base T (0/11);  total (148/146)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 297 total aligned reads displayed.

CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGT  >  CP009273/3535223‑3535516
                                                                                                                                                   |                                                                                                                                                  
cATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                                  <  1:1171732/150‑1 (MQ=255)
cATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                                  <  2:2902089/150‑1 (MQ=255)
 aTGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                 <  1:622983/150‑1 (MQ=255)
      gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAt                                                                                                                                            <  2:2945211/150‑1 (MQ=255)
       ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtc                                                                                                                                           <  2:2544646/150‑1 (MQ=255)
        tGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcg                                                                                                                                          <  2:4437499/150‑1 (MQ=255)
          ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtc                                                                                                                                        <  1:892983/150‑1 (MQ=255)
          ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtc                                                                                                                                        <  1:2513099/150‑1 (MQ=255)
            ggTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGa                                                                                                                                      >  1:448778/1‑150 (MQ=255)
              tCTATCATGTACCACACGTTAACAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATa                                                                                                                                    <  2:217180/150‑1 (MQ=255)
              tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATa                                                                                                                                    >  2:724014/1‑150 (MQ=255)
              tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATa                                                                                                                                    >  2:400553/1‑150 (MQ=255)
                 aTCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACa                                                                                                                                 >  2:916113/1‑150 (MQ=255)
                  tCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACAt                                                                                                                                <  1:1843725/150‑1 (MQ=255)
                    aTGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                              <  2:4049280/150‑1 (MQ=255)
                    aTGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                              <  1:1421569/150‑1 (MQ=255)
                    aTGTACCACACGTTAAAAGACTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                              >  1:1294417/1‑150 (MQ=255)
                      gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAc                                                                                                                            >  1:966463/1‑150 (MQ=255)
                        aCCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATCACATCAACGa                                                                                                                          >  1:3432284/1‑150 (MQ=255)
                         ccACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGAc                                                                                                                         >  2:5060261/1‑150 (MQ=255)
                         ccACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGAc                                                                                                                         >  1:349521/1‑150 (MQ=255)
                          cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACt                                                                                                                        <  2:2000074/150‑1 (MQ=255)
                              cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgc                                                                                                                    >  1:3605251/1‑150 (MQ=255)
                                  aaaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGTTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTg                                                                                                                >  2:3832974/1‑150 (MQ=255)
                                   aaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTgg                                                                                                               <  2:2961951/150‑1 (MQ=255)
                                    aaGCCTGATTATCCAGACCTGGCAGCGTCTTAATGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGATTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGc                                                                                                              <  1:2724261/150‑1 (MQ=255)
                                     aGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcg                                                                                                             >  1:2122797/1‑150 (MQ=255)
                                      gCCTGTTTATCAATACCGGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGTTAAAGTTATCATCATCTTCTGCTTTTTCCTGTGCGCTTTAAACAGCTTCTCGCTGATAGGTAAAATGGTCTATAACTTCAACGACTCGGCGCTGGcga                                                                                                            <  2:2278799/150‑2 (MQ=255)
                                      gCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgt                                                                                                            <  2:2098155/150‑1 (MQ=255)
                                          gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgt                                                                                                        >  2:1933806/1‑150 (MQ=255)
                                           aTTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgtc                                                                                                       <  2:2043987/150‑1 (MQ=255)
                                            ttATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCa                                                                                                      >  2:1154628/1‑150 (MQ=255)
                                              aTCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGc                                                                                                    >  1:3330721/1‑150 (MQ=255)
                                                 cAGCCCTGGCAGCGTCTGAACGGCTTCGTTCTGCGTGCTGGTAATGTGATCATCATCGTCAGAATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGTAAAATCGTCGATAACAACCACGACTCGGCGCTGGCGTCCGTCAGCCgg                                                                                                 <  1:1523220/150‑1 (MQ=255)
                                                 cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCgg                                                                                                 >  2:4251090/1‑150 (MQ=255)
                                                 cAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCgg                                                                                                 >  1:1929426/1‑150 (MQ=255)
                                                  aGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCggg                                                                                                >  1:486726/1‑150 (MQ=255)
                                                    aCCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTg                                                                                              <  2:3676894/150‑1 (MQ=255)
                                                     ccTGGCAGCGTCTGAACGGCTTCGTTCTGCGTGCTGTTAATTTGATCATAATCGTCACCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGa                                                                                             <  1:1771636/150‑1 (MQ=255)
                                                      cTGGTAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGAt                                                                                            <  2:4699315/150‑1 (MQ=255)
                                                        ggCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCa                                                                                          >  1:1083461/1‑150 (MQ=255)
                                                           aGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAccc                                                                                       >  1:685532/1‑150 (MQ=255)
                                                             cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCg                                                                                     >  2:4133171/1‑150 (MQ=255)
                                                             cGTCTGAAAGGCTTCGTGCTGCGTGCTGGTAACGTGATCATCATCGGCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGGGATCACCCCg                                                                                     <  2:861432/150‑1 (MQ=255)
                                                               tCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGt                                                                                   >  1:2331730/1‑150 (MQ=255)
                                                                 tGAAAGGCTTCGTTCTGCGTGGTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGCGCGCTTACAACAGCTTCTCGCTGAGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCt                                                                                 <  1:4371554/150‑1 (MQ=255)
                                                                 tGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCt                                                                                 >  1:4777939/1‑150 (MQ=255)
                                                                   aacggCTTCGTTCTGCGTGCTGTTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGACGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTc                                                                               <  1:184610/147‑1 (MQ=255)
                                                                   aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTc                                                                               >  2:4338920/1‑150 (MQ=255)
                                                                    aaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCa                                                                              <  1:409875/150‑1 (MQ=255)
                                                                        cTTCGTTCTGCGTGCTGTTAATGTTATCATCTTCGTCAGCTTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGcc                                                                          <  1:1884524/150‑1 (MQ=255)
                                                                         ttCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCa                                                                         >  2:31103/1‑150 (MQ=255)
                                                                         ttCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCa                                                                         >  2:3985430/1‑150 (MQ=255)
                                                                         ttCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCa                                                                         >  1:2102507/1‑150 (MQ=255)
                                                                          tCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCaa                                                                        <  2:643021/150‑1 (MQ=255)
                                                                           cGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAt                                                                       >  1:3730159/1‑150 (MQ=255)
                                                                            gTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAtt                                                                      <  2:448778/150‑1 (MQ=255)
                                                                             ttCTGCGTGATGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTg                                                                     <  2:1529929/150‑1 (MQ=255)
                                                                               cTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGc                                                                   >  2:4816035/1‑150 (MQ=255)
                                                                               cTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGc                                                                   <  2:1235764/150‑1 (MQ=255)
                                                                                tGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCg                                                                  >  1:1133348/1‑150 (MQ=255)
                                                                                  cGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGCGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTg                                                                >  2:3225727/1‑150 (MQ=255)
                                                                                    tGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCa                                                              >  2:534900/1‑150 (MQ=255)
                                                                                      cTGGTAATGTGATCATCATCGTCAGCTTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGTAAATTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATg                                                            <  1:4956127/150‑1 (MQ=255)
                                                                                        gttAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGGTGTGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGaa                                                          <  1:1062972/148‑1 (MQ=255)
                                                                                           aaaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGAt                                                       <  1:400553/150‑1 (MQ=255)
                                                                                            aaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATa                                                      >  2:4979331/1‑150 (MQ=255)
                                                                                            aaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATa                                                      <  1:2870393/150‑1 (MQ=255)
                                                                                             aGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATaa                                                     >  1:755712/1‑150 (MQ=255)
                                                                                              gTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCCTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAATGGCGTGCATGAAGATAAc                                                    >  1:4758013/1‑150 (MQ=255)
                                                                                               tGATCATCATCGTCAGCATTTTCCGGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACt                                                   <  2:1435436/150‑1 (MQ=255)
                                                                                                 atcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTgg                                                 >  2:2370632/1‑150 (MQ=255)
                                                                                                 atcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTgg                                                 >  1:573491/1‑150 (MQ=255)
                                                                                                  tcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                >  2:3538416/1‑150 (MQ=255)
                                                                                                   catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggca                                               <  1:2434462/150‑1 (MQ=255)
                                                                                                       atcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggca                                           >  2:2766561/1‑150 (MQ=255)
                                                                                                       atcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggca                                           <  1:4312876/150‑1 (MQ=255)
                                                                                                       atcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggca                                           >  2:1684643/1‑150 (MQ=255)
                                                                                                       atcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggca                                           >  2:3752265/1‑150 (MQ=255)
                                                                                                         cGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                         >  1:4604915/1‑150 (MQ=255)
                                                                                                           tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACgg                                       <  1:3832974/150‑1 (MQ=255)
                                                                                                               cATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTgg                                   <  2:2220168/150‑1 (MQ=255)
                                                                                                                  tttCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCt                                <  1:4144013/150‑1 (MQ=255)
                                                                                                                    tCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGt                              >  2:3338755/1‑150 (MQ=255)
                                                                                                                     ccTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATGGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGtt                             <  2:1623560/150‑1 (MQ=255)
                                                                                                                       tGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                           <  2:1083133/150‑1 (MQ=255)
                                                                                                                         aGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACa                         >  1:256305/1‑150 (MQ=255)
                                                                                                                           cgcTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAgg                       >  1:3915253/1‑150 (MQ=255)
                                                                                                                             cTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAACCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                     >  1:3246884/1‑150 (MQ=255)
                                                                                                                                tCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCa                  >  2:4909137/1‑150 (MQ=255)
                                                                                                                                 cAACCGCTTCTCGCTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCAt                 <  2:1903006/150‑1 (MQ=255)
                                                                                                                                  aaCAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATg                >  2:3902360/1‑150 (MQ=255)
                                                                                                                                       cgtctcGCTGTGAGGGAAAATCGTCGATAACCTCAACGACTCGGCGCGGGCGTCCGTCAGCCGGGTGATCACCCCGGTCGTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa           <  2:4415119/148‑1 (MQ=255)
                                                                                                                                            cGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag      >  1:2580731/1‑150 (MQ=255)
                                                                                                                                             gCTGAGAGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGt     <  2:4887572/150‑1 (MQ=255)
                                                                                                                                              cTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGGTAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg    >  1:3064958/1‑150 (MQ=255)
                                                                                                                                               tGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTgg   <  2:3391623/150‑1 (MQ=255)
                                                                                                                                               tGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAGTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTgg   <  2:5128990/150‑1 (MQ=255)
                                                                                                                                                gagaGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt  <  2:1294417/150‑1 (MQ=255)
                                                                                                                                                gAGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt  <  1:5106090/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGT  >  CP009273/3535223‑3535516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑