Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,596,266 C→A 100% T446T (ACG→ACT)  ftsY ← Signal Recognition Particle (SRP) receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,596,2660CA98.7% 992.3 / 2.4 303T446T (ACG→ACT) ftsYSignal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (132/166);  total (135/168)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 307 total aligned reads displayed.

CGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTT  >  CP009273/3596120‑3596412
                                                                                                                                                  |                                                                                                                                                  
cGGGCAAACAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGtt                                                                                                                                                 <  1:2069924/150‑1 (MQ=255)
cGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGtt                                                                                                                                                 <  2:1487723/150‑1 (MQ=255)
 gggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTa                                                                                                                                                >  2:2405892/1‑150 (MQ=255)
  ggCAAAAAGTGCCTCTATAACGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGGTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTttagttag                                                                                                                                               <  1:4405991/150‑1 (MQ=255)
  ggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTttagttag                                                                                                                                               <  1:1834687/150‑1 (MQ=255)
  ggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTttagttag                                                                                                                                               <  1:3476863/150‑1 (MQ=255)
  ggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTttagttag                                                                                                                                               >  2:4144469/1‑150 (MQ=255)
    cAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCACTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCg                                                                                                                                             <  2:3944681/150‑1 (MQ=255)
    cAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTATCGCCGTGCCGTCCAGTTTAGTTAGCg                                                                                                                                             >  2:4062319/1‑150 (MQ=255)
      aaaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTg                                                                                                                                           >  2:441900/1‑150 (MQ=255)
       aaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGa                                                                                                                                          >  1:1686342/1‑150 (MQ=255)
        aaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGAt                                                                                                                                         <  1:564642/150‑1 (MQ=255)
          gTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGc                                                                                                                                       >  2:4813743/1‑150 (MQ=255)
          gTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGc                                                                                                                                       >  2:2811483/1‑150 (MQ=255)
           tGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGcc                                                                                                                                      <  1:4645807/150‑1 (MQ=255)
               tctATACAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCACACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGtt                                                                                                                                  <  2:4684286/150‑1 (MQ=255)
               tctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGtt                                                                                                                                  <  2:1893311/150‑1 (MQ=255)
                ctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCACTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTa                                                                                                                                 <  1:2884321/150‑1 (MQ=255)
                ctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTa                                                                                                                                 <  2:3384490/150‑1 (MQ=255)
                  ataAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAg                                                                                                                               >  1:3039550/1‑150 (MQ=255)
                   taAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGc                                                                                                                              >  2:4979228/1‑150 (MQ=255)
                      aGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCaa                                                                                                                           >  1:2825562/1‑150 (MQ=255)
                      aGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCaa                                                                                                                           >  1:640021/1‑150 (MQ=255)
                         cgtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACgg                                                                                                                        >  1:3033921/1‑150 (MQ=255)
                              gCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCa                                                                                                                   <  2:4629064/150‑1 (MQ=255)
                                cTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATg                                                                                                                 >  2:1194839/1‑150 (MQ=255)
                                 ttAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATng                                                                                                                <  1:1417652/150‑1 (MQ=255)
                                   aaaCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGaa                                                                                                              >  2:1246711/1‑150 (MQ=255)
                                   aaaCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGaa                                                                                                              <  1:4362142/150‑1 (MQ=255)
                                   aaaCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGaa                                                                                                              >  1:3076543/1‑150 (MQ=255)
                                      cGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTATCGGATAGGGATACCAAACTGGTCAGAAAACGAGAAAATTACCCCGCATTTCTCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAAAGGCTTCATGGACCAg                                                                                                           >  1:4849505/1‑150 (MQ=255)
                                         aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGttt                                                                                                        >  1:1820984/1‑150 (MQ=255)
                                          cGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTg                                                                                                       <  1:2361664/150‑1 (MQ=255)
                                          cGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTg                                                                                                       <  2:1271342/150‑1 (MQ=255)
                                                 ccTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTGCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGc                                                                                                <  2:1679465/150‑1 (MQ=255)
                                                   tCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCtt                                                                                              <  1:2139728/150‑1 (MQ=255)
                                                    cactccGTGCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTa                                                                                             <  2:1855146/145‑1 (MQ=255)
                                                    cAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTa                                                                                             <  2:30616/150‑1 (MQ=255)
                                                    cAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTa                                                                                             >  1:4076985/1‑150 (MQ=255)
                                                        aCGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACcgc                                                                                         <  1:952630/150‑1 (MQ=255)
                                                         cGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACcgcg                                                                                        <  2:2187693/150‑1 (MQ=255)
                                                          gTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGt                                                                                       <  1:1357125/150‑1 (MQ=255)
                                                            tCGCCGACACCAATGTAGCGGATAGGGATACCAACCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTc                                                                                     <  2:5068579/150‑1 (MQ=255)
                                                            tCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTc                                                                                     <  1:3094173/150‑1 (MQ=255)
                                                             cGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCt                                                                                    >  1:3902257/1‑150 (MQ=255)
                                                              gCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTg                                                                                   >  1:1228501/1‑150 (MQ=255)
                                                              gCCGACACCAATGGAGCGGATAGGGATACCAAACTTGTCAGCCACCGAGAAAATTACCCCGCCTTTAGCCGTGCCGTCCAGTTTAGTTAGCGTTATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTg                                                                                   <  1:936490/150‑1 (MQ=255)
                                                                cGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                                                 <  1:2702931/150‑1 (MQ=255)
                                                                 gACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGccc                                                                                <  1:1015591/150‑1 (MQ=255)
                                                                      cAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGc                                                                           <  2:2268827/150‑1 (MQ=255)
                                                                      cAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGc                                                                           <  1:4761403/150‑1 (MQ=255)
                                                                      cAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGc                                                                           <  1:1648243/150‑1 (MQ=255)
                                                                       aaTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                                                          <  2:3769094/150‑1 (MQ=255)
                                                                         tGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGACTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTgg                                                                        >  2:3707840/1‑150 (MQ=255)
                                                                          gTAGCGGATAGGGATACCACACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGc                                                                       <  2:102242/150‑1 (MQ=255)
                                                                           tAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCa                                                                      >  2:1369337/1‑150 (MQ=255)
                                                                            aGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGcgg                                                                     >  1:2611320/1‑148 (MQ=255)
                                                                              cgtaTAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCa                                                                   <  1:522198/147‑1 (MQ=255)
                                                                                gATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAt                                                                 <  2:511893/150‑1 (MQ=255)
                                                                                           cAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATaa                                                      <  2:3446040/150‑1 (MQ=255)
                                                                                            aaaCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAAc                                                     >  1:3363853/1‑150 (MQ=255)
                                                                                            aaaCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAAc                                                     <  2:4185886/150‑1 (MQ=255)
                                                                                              aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACtt                                                   <  1:4521240/150‑1 (MQ=255)
                                                                                               cTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTc                                                  <  1:1929165/150‑1 (MQ=255)
                                                                                               cTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTc                                                  <  2:319066/150‑1 (MQ=255)
                                                                                                    cAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcg                                             >  1:4523745/1‑150 (MQ=255)
                                                                                                       ccACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcggcg                                          <  2:2500107/150‑1 (MQ=255)
                                                                                                           cGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTc                                      >  1:2779145/1‑150 (MQ=255)
                                                                                                                 aaTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTc                                <  1:814498/150‑1 (MQ=255)
                                                                                                                 aaTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTc                                <  2:639928/150‑1 (MQ=255)
                                                                                                                  aTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTTGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCg                               <  1:3366230/150‑1 (MQ=255)
                                                                                                                   ttACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGa                              <  2:4944096/150‑1 (MQ=255)
                                                                                                                    tACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAg                             >  1:4223548/1‑150 (MQ=255)
                                                                                                                    tACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAg                             <  1:3628819/150‑1 (MQ=255)
                                                                                                                      ccccGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGtt                           <  2:2934074/150‑1 (MQ=255)
                                                                                                                       cccGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGttt                          <  2:1058458/150‑1 (MQ=255)
                                                                                                                        ccGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttc                         >  2:581057/1‑150 (MQ=255)
                                                                                                                        ccGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttc                         <  2:3999358/150‑1 (MQ=255)
                                                                                                                         cGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttct                        <  1:5084632/150‑1 (MQ=255)
                                                                                                                            cTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtca                     >  2:1399012/1‑150 (MQ=255)
                                                                                                                            cTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtca                     <  1:4044149/150‑1 (MQ=255)
                                                                                                                               tCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatca                  >  2:202060/1‑150 (MQ=255)
                                                                                                                                cGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAc                 <  2:3605492/150‑1 (MQ=255)
                                                                                                                                cGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAc                 >  2:2326549/1‑150 (MQ=255)
                                                                                                                                cGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAc                 >  1:3438864/1‑150 (MQ=255)
                                                                                                                                   cgtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgcg              >  1:4901870/1‑150 (MQ=255)
                                                                                                                                    ttgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCgg             >  2:957845/2‑150 (MQ=255)
                                                                                                                                    gtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCgg             <  2:4530310/150‑1 (MQ=255)
                                                                                                                                    gtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCgg             >  1:919711/1‑150 (MQ=255)
                                                                                                                                    gtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCgg             >  1:480260/1‑150 (MQ=255)
                                                                                                                                       ccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACg          <  2:1820984/150‑1 (MQ=255)
                                                                                                                                       ccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACg          >  1:1079225/1‑150 (MQ=255)
                                                                                                                                        cgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGa         >  1:5015437/1‑150 (MQ=255)
                                                                                                                                        cgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGa         >  1:4108106/1‑150 (MQ=255)
                                                                                                                                        cgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGa         >  1:202597/1‑150 (MQ=255)
                                                                                                                                          tCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAtt       <  2:3838613/150‑1 (MQ=255)
                                                                                                                                            cAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAtttt     <  1:2523040/150‑1 (MQ=255)
                                                                                                                                             aGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttc    >  2:2613062/1‑150 (MQ=255)
                                                                                                                                             aGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttc    >  1:4956864/1‑150 (MQ=255)
                                                                                                                                              gTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttct   <  2:1759424/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTCTT  >  CP009273/3596120‑3596412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑