Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,639,239 G→T 100% P165P (CCG→CCT)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,639,2390GT96.4% 817.4 / 15.8 266P165P (CCG→CCT) rlmJ23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (8/2);  new base T (138/116);  total (148/118)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 272 total aligned reads displayed.

GACCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCG  >  CP009273/3639092‑3639387
                                                                                                                                                   |                                                                                                                                                    
gACCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACcctcc                                                                                                                                                    <  1:442161/150‑1 (MQ=255)
    gAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTTAATTTCAGAAAGATAGCAGTGCGCGTGTCGTAAAAGCCGACTGTTTCCAGCAGCTTAAGGCCAAACTGCCGCAGGTTTCCAGACGTTGTTTAATCCTTATCGACCCGCCGTat                                                                                                                                                <  1:678179/150‑1 (MQ=255)
     aaCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatg                                                                                                                                               <  1:2710233/150‑1 (MQ=255)
          cACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaatg                                                                                                                                          >  2:4474127/1‑150 (MQ=255)
             ccGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTAtgaaatgaaa                                                                                                                                       >  1:2505323/1‑150 (MQ=255)
             ccGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTAtgaaatgaaa                                                                                                                                       <  1:4298652/150‑1 (MQ=255)
                 gCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTg                                                                                                                                   <  2:3748412/150‑1 (MQ=255)
                   gATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGAc                                                                                                                                 <  2:495374/150‑1 (MQ=255)
                      tACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTAt                                                                                                                              <  1:1615431/150‑1 (MQ=255)
                      tACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTAt                                                                                                                              >  1:1070590/1‑150 (MQ=255)
                       aCCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATc                                                                                                                             >  2:4508244/1‑150 (MQ=255)
                          cGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAg                                                                                                                          >  1:4677203/1‑150 (MQ=255)
                             tgttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCgg                                                                                                                       >  1:2278453/1‑150 (MQ=255)
                              gttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggt                                                                                                                      <  1:3681585/150‑1 (MQ=255)
                               ttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggtg                                                                                                                     <  1:4593592/150‑1 (MQ=255)
                               ttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggtg                                                                                                                     <  2:413502/150‑1 (MQ=255)
                                 gCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggtggt                                                                                                                   <  1:2439685/150‑1 (MQ=255)
                                    ttCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAg                                                                                                                <  1:1007611/150‑1 (MQ=255)
                                     tCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGc                                                                                                               <  1:2542297/150‑1 (MQ=255)
                                      cTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCg                                                                                                              <  2:3586288/150‑1 (MQ=255)
                                       tGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCgg                                                                                                             >  1:861191/1‑150 (MQ=255)
                                        gAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCggg                                                                                                            <  2:1393104/150‑1 (MQ=255)
                                         aaTTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGa                                                                                                           >  1:5142817/1‑150 (MQ=255)
                                         aaTTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGa                                                                                                           >  1:3200366/1‑150 (MQ=255)
                                           tttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTAACAAGCGGTGGTCAGCGGGATa                                                                                                         <  1:1266806/150‑1 (MQ=255)
                                            ttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAg                                                                                                        <  2:4806875/150‑1 (MQ=255)
                                            ttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAg                                                                                                        <  2:3238792/150‑1 (MQ=255)
                                              cAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCa                                                                                                      >  2:210961/1‑150 (MQ=255)
                                               aGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAg                                                                                                     <  1:2099982/150‑1 (MQ=255)
                                                gAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGa                                                                                                    <  1:4355306/150‑1 (MQ=255)
                                                 aaaGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGaa                                                                                                   >  2:1364788/1‑150 (MQ=255)
                                                  aaGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAg                                                                                                  <  1:3354646/150‑1 (MQ=255)
                                                  aaGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAg                                                                                                  <  1:4432254/150‑1 (MQ=255)
                                                   aGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAgg                                                                                                 <  1:3089339/150‑1 (MQ=255)
                                                    gATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGt                                                                                                <  1:904986/150‑1 (MQ=255)
                                                      tAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTa                                                                                              <  2:253417/150‑1 (MQ=255)
                                                      tAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTa                                                                                              <  2:2728895/150‑1 (MQ=255)
                                                          cGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACaaa                                                                                          >  2:4059573/1‑150 (MQ=255)
                                                                cgTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTc                                                                                    >  1:3342262/1‑150 (MQ=255)
                                                                      gAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                              >  1:4038842/1‑150 (MQ=255)
                                                                       aaaaaGCCGACGGTTTCCAGCAGCTTAAGGCCAACCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTg                                                                             <  2:4919371/150‑1 (MQ=255)
                                                                       aaaaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTg                                                                             >  2:2000120/1‑150 (MQ=255)
                                                                         aaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGt                                                                           >  1:145322/1‑150 (MQ=255)
                                                                          aaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTa                                                                          >  2:3980806/1‑150 (MQ=255)
                                                                           aGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                         <  2:3842540/150‑1 (MQ=255)
                                                                            gCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAtt                                                                        <  2:2079881/150‑1 (MQ=255)
                                                                             ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAttt                                                                       <  2:1641174/150‑1 (MQ=255)
                                                                             ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAttt                                                                       <  2:654453/150‑1 (MQ=255)
                                                                             ccGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGGGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAttt                                                                       >  1:3730489/1‑150 (MQ=255)
                                                                              cGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTa                                                                      >  1:1671420/1‑150 (MQ=255)
                                                                              cGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTa                                                                      >  2:2274/1‑150 (MQ=255)
                                                                                  ggTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                                  >  1:2043395/1‑150 (MQ=255)
                                                                                     ttCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtg                                                               <  2:109706/150‑1 (MQ=255)
                                                                                         agcagcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGt                                                           >  1:315749/1‑150 (MQ=255)
                                                                                            agcTTAAGTCCAAACTGCCGCCGGTTTCCAGCCGTGGTTTAATCATTATCTACCCTCCTTATGAAATGAAAACTGAGTTTCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATc                                                        <  1:1236747/150‑1 (MQ=255)
                                                                                             gcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGCCCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATcc                                                       <  2:639606/150‑1 (MQ=255)
                                                                                             gcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATcc                                                       >  1:3603333/1‑150 (MQ=255)
                                                                                              cTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGACATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATcca                                                      <  1:3290976/150‑2 (MQ=255)
                                                                                              cTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCg                                                      >  2:3059848/1‑150 (MQ=255)
                                                                                              cTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCg                                                      >  1:2292963/1‑150 (MQ=255)
                                                                                              cTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACACTCAGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGAGGGATAGCAGAAGGATACAAACGATTCTCCACTGGTATTTACGCAATGTGGTATCCg                                                      >  2:2238321/1‑150 (MQ=255)
                                                                                               ttAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCgg                                                     >  1:2146300/1‑150 (MQ=255)
                                                                                                tAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggt                                                    >  2:1919083/1‑150 (MQ=255)
                                                                                                 aaGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtg                                                   >  2:1698360/1‑150 (MQ=255)
                                                                                                  aGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTCTCCggtgg                                                  >  2:5060413/1‑150 (MQ=255)
                                                                                                   ggCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtggt                                                 <  1:976614/150‑1 (MQ=255)
                                                                                                     ccAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgc                                               <  1:4221971/150‑1 (MQ=255)
                                                                                                     ccAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgc                                               >  2:1586187/1‑150 (MQ=255)
                                                                                                      cAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgct                                              <  1:1857723/150‑1 (MQ=255)
                                                                                                      cAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgct                                              >  2:2467853/1‑150 (MQ=255)
                                                                                                      cAAACTGCCGCCGGTGTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgct                                              <  2:2282698/150‑1 (MQ=255)
                                                                                                       aaaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGGTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctg                                             >  2:490033/1‑150 (MQ=255)
                                                                                                        aaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctgc                                            <  2:315550/150‑1 (MQ=255)
                                                                                                         aCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCg                                           <  2:3166602/150‑1 (MQ=255)
                                                                                                          cTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGt                                          >  1:614306/1‑150 (MQ=255)
                                                                                                            gccgccGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCa                                        <  1:4999657/150‑1 (MQ=255)
                                                                                                             ccgccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAg                                       <  2:3429467/150‑1 (MQ=255)
                                                                                                             ccgccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAg                                       <  2:3318262/150‑1 (MQ=255)
                                                                                                               gccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCa                                     >  2:2325628/1‑150 (MQ=255)
                                                                                                                ccgGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCaa                                    >  2:1054755/1‑150 (MQ=255)
                                                                                                                  ggTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                                  >  1:1394294/1‑150 (MQ=255)
                                                                                                                    tttCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTa                                >  2:1275137/1‑150 (MQ=255)
                                                                                                                     ttCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTaa                               >  2:2963345/1‑150 (MQ=255)
                                                                                                                     ttCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTaa                               >  2:3924259/1‑150 (MQ=255)
                                                                                                                      tCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAg                              <  1:3753710/150‑1 (MQ=255)
                                                                                                                       cccGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgc                             >  1:536505/1‑150 (MQ=255)
                                                                                                                       cccGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgc                             >  1:4767111/1‑150 (MQ=255)
                                                                                                                       cccGCCGAGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgc                             >  2:889039/1‑150 (MQ=255)
                                                                                                                            cgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATg                        <  1:3484341/150‑1 (MQ=255)
                                                                                                                            cgTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATg                        >  1:2287423/1‑150 (MQ=255)
                                                                                                                               ggTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                     >  2:734160/1‑150 (MQ=255)
                                                                                                                               ggTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                     >  1:2153107/1‑150 (MQ=255)
                                                                                                                                gTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATcc                    <  1:4717621/150‑1 (MQ=255)
                                                                                                                                      tCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATc              >  1:2817415/1‑150 (MQ=255)
                                                                                                                                       ccTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCt             >  2:1306064/1‑150 (MQ=255)
                                                                                                                                          tATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGa          >  2:3698028/1‑150 (MQ=255)
                                                                                                                                               aCCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGa     <  2:2092211/150‑1 (MQ=255)
                                                                                                                                               aCCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGa     >  1:1152952/1‑150 (MQ=255)
                                                                                                                                               aCCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGa     >  1:3131250/1‑150 (MQ=255)
                                                                                                                                               aCCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGa     >  1:300237/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GACCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCG  >  CP009273/3639092‑3639387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑