Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,657,375 G→A 100% P202S (CCT→TCT)  gadW ← transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,657,3750GA98.2% 946.0 / 3.5 284P202S (CCT→TCT) gadWtranscriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base A (147/132);  total (149/135)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 287 total aligned reads displayed.

CAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATAC  >  CP009273/3657228‑3657520
                                                                                                                                                   |                                                                                                                                                 
cAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGGAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGaaa                                                                                                                                                 >  2:2398648/1‑150 (MQ=255)
      tCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGAc                                                                                                                                           <  1:1273390/150‑1 (MQ=255)
       cAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGAca                                                                                                                                          <  1:1450883/150‑2 (MQ=255)
          aTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTaa                                                                                                                                       <  2:1776936/150‑1 (MQ=255)
          aTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTaa                                                                                                                                       >  1:1744642/1‑150 (MQ=255)
            tcGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACt                                                                                                                                     >  2:2016813/2‑150 (MQ=255)
            gCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACt                                                                                                                                     <  2:193099/150‑1 (MQ=255)
             cGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTc                                                                                                                                    >  1:4139137/1‑150 (MQ=255)
             cGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTc                                                                                                                                    >  2:4269393/1‑150 (MQ=255)
              gAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCg                                                                                                                                   >  2:791226/1‑150 (MQ=255)
              gAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCg                                                                                                                                   <  2:449384/150‑1 (MQ=255)
              gAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCg                                                                                                                                   >  2:4894811/1‑150 (MQ=255)
                 atatGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGt                                                                                                                                >  1:3881725/1‑150 (MQ=255)
                 atatGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGt                                                                                                                                >  2:1428724/1‑150 (MQ=255)
                 atatGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGAATGCAGAGAAATTTGACGTAACTCGAGt                                                                                                                                >  2:4772755/1‑150 (MQ=255)
                       cAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGt                                                                                                                          >  2:63338/1‑150 (MQ=255)
                               ggTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCAt                                                                                                                  <  1:1730827/150‑1 (MQ=255)
                                gTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATc                                                                                                                 <  1:3441842/150‑1 (MQ=255)
                                 tACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCg                                                                                                                >  2:1175049/1‑150 (MQ=255)
                                   ccTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGAc                                                                                                              >  2:598785/1‑150 (MQ=255)
                                   ccTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGAc                                                                                                              >  1:962180/1‑150 (MQ=255)
                                     tGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACAt                                                                                                            >  1:4519855/1‑150 (MQ=255)
                                      ggCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATc                                                                                                           >  1:4319191/1‑150 (MQ=255)
                                       tcGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTCTAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAAATCGAGTAATCGTCTGGACATCGACATcc                                                                                                          >  2:2613369/2‑150 (MQ=255)
                                       gCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCTTCTGGCCATCGACATcc                                                                                                          >  1:438400/1‑150 (MQ=255)
                                       gCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATcc                                                                                                          <  2:880478/150‑1 (MQ=255)
                                       gCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATca                                                                                                          <  2:4226506/150‑2 (MQ=255)
                                        cGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCt                                                                                                         <  2:2719670/150‑1 (MQ=255)
                                        cGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCt                                                                                                         <  1:3814594/150‑1 (MQ=255)
                                         gAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTg                                                                                                        >  1:2634105/1‑150 (MQ=255)
                                         gAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTg                                                                                                        <  1:872048/150‑1 (MQ=255)
                                         gAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTg                                                                                                        >  1:29306/1‑150 (MQ=255)
                                         gAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGATATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTg                                                                                                        >  1:4881997/1‑150 (MQ=255)
                                           aTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGa                                                                                                      >  2:1510924/1‑150 (MQ=255)
                                            tGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAg                                                                                                     <  2:365673/150‑1 (MQ=255)
                                                   cAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTa                                                                                              >  1:4716826/1‑150 (MQ=255)
                                                    aaaCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAATTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGtca                                                                                             >  2:3341224/1‑148 (MQ=255)
                                                      aCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAata                                                                                           <  2:1694231/150‑1 (MQ=255)
                                                      aCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAata                                                                                           <  2:3838370/150‑1 (MQ=255)
                                                       cTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAatat                                                                                          <  1:3567635/150‑1 (MQ=255)
                                                       cTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAatat                                                                                          >  1:3306434/1‑150 (MQ=255)
                                                        tGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATAtt                                                                                         <  1:506165/150‑1 (MQ=255)
                                                           tgtgGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTa                                                                                      >  1:1348052/1‑150 (MQ=255)
                                                               gCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATTTACTGCTATAGCCACAAATTTCCGCAATAGTATGCAGAGAAATATGACGTAAATCGAGAAATCGTCTGGCCATCGACATCCTGGAGGCGAGAAATAATTTACTGa                                                                                  >  1:3749003/1‑150 (MQ=255)
                                                               gCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGa                                                                                  >  1:3199335/1‑150 (MQ=255)
                                                                 gTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                                >  1:3380744/1‑150 (MQ=255)
                                                                 gTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                                >  1:2285821/1‑150 (MQ=255)
                                                                  ttACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAAc                                                                               <  2:2533259/150‑1 (MQ=255)
                                                                  ttACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAAc                                                                               <  2:2730946/150‑1 (MQ=255)
                                                                        cATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTAt                                                                         >  2:3287220/1‑150 (MQ=255)
                                                                        cATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTAt                                                                         >  2:702331/1‑150 (MQ=255)
                                                                          taataTTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTAttt                                                                       <  1:4201375/150‑1 (MQ=255)
                                                                                  tCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTg                                                               <  1:1817578/150‑1 (MQ=255)
                                                                                   cGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGc                                                              >  2:4856823/1‑150 (MQ=255)
                                                                                     aaaTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCaa                                                            >  2:4056138/1‑150 (MQ=255)
                                                                                     aaaTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCaa                                                            <  1:2856306/150‑1 (MQ=255)
                                                                                         gtgtTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACttt                                                        >  2:505904/1‑150 (MQ=255)
                                                                                         gtgtTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGCAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACttt                                                        <  1:3570044/150‑1 (MQ=255)
                                                                                              tataAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtttttttt                                                   >  2:2103657/1‑150 (MQ=255)
                                                                                               ataAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTg                                                  >  1:2426451/1‑150 (MQ=255)
                                                                                                taAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGa                                                 >  1:4776102/1‑150 (MQ=255)
                                                                                                taAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGa                                                 <  2:1836571/150‑1 (MQ=255)
                                                                                                taAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGa                                                 >  1:2543247/1‑150 (MQ=255)
                                                                                                   aGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTa                                              <  1:5143572/150‑1 (MQ=255)
                                                                                                   aGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTa                                              >  1:2765170/1‑150 (MQ=255)
                                                                                                      aCGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGAc                                           <  2:3709578/150‑1 (MQ=255)
                                                                                                       cGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGAct                                          <  2:3749080/150‑1 (MQ=255)
                                                                                                        gATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGActc                                         >  2:288001/1‑150 (MQ=255)
                                                                                                        gATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGActc                                         >  2:2275586/1‑150 (MQ=255)
                                                                                                        gATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATAAGActc                                         >  1:5108185/1‑150 (MQ=255)
                                                                                                         aTGTACTGCTATAGCCAAATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATAGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTTTAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGActct                                        <  1:4269393/150‑1 (MQ=255)
                                                                                                          tGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACtctc                                       <  1:1871078/150‑1 (MQ=255)
                                                                                                           gTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCg                                      >  1:590351/1‑150 (MQ=255)
                                                                                                             aCTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCt                                    >  2:2247409/1‑150 (MQ=255)
                                                                                                                 cTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGta                                >  1:268403/1‑150 (MQ=255)
                                                                                                                 cTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGta                                <  1:2015168/150‑1 (MQ=255)
                                                                                                                 cTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGta                                >  1:232745/1‑150 (MQ=255)
                                                                                                                   ataGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGtata                              >  2:342812/1‑150 (MQ=255)
                                                                                                                   ataGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGtata                              >  2:4876120/1‑150 (MQ=255)
                                                                                                                   ataGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGtata                              >  1:4935631/1‑150 (MQ=255)
                                                                                                                    taGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATAc                             >  2:2989000/1‑150 (MQ=255)
                                                                                                                       ccACATTTTTCCGCAATAGTATGCAGAGAAATTTAACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGAAACTTTTTTTTGATTAGACTCTCGCTGGGAGACAtt                          >  2:1059005/1‑150 (MQ=255)
                                                                                                                             ttttCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAACCAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTcc                    <  2:3401604/150‑1 (MQ=255)
                                                                                                                                 ccGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGa                >  1:1051141/1‑150 (MQ=255)
                                                                                                                                    cAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGatat             <  2:911950/150‑1 (MQ=255)
                                                                                                                                     aaTAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATc            >  1:4626485/1‑150 (MQ=255)
                                                                                                                                     aaTAGTATGCAGAGAAAATTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATc            <  2:2949576/150‑1 (MQ=255)
                                                                                                                                      aTAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcg           <  2:1589930/150‑1 (MQ=255)
                                                                                                                                      aTAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcg           <  1:3630783/150‑1 (MQ=255)
                                                                                                                                      aTAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcg           <  1:2473736/150‑1 (MQ=255)
                                                                                                                                      aTAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcg           >  1:1415540/1‑150 (MQ=255)
                                                                                                                                        aGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcgcg         <  2:4319286/150‑1 (MQ=255)
                                                                                                                                        aGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATACTTTCCGCGATATcgcg         >  1:2188471/1‑150 (MQ=255)
                                                                                                                                            tGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGa     >  1:634566/1‑150 (MQ=255)
                                                                                                                                            tGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGa     <  1:3061384/150‑1 (MQ=255)
                                                                                                                                            tGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGa     >  1:1542106/1‑150 (MQ=255)
                                                                                                                                             gCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGAt    <  1:2387743/150‑1 (MQ=255)
                                                                                                                                             gCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGAt    <  1:1175049/150‑1 (MQ=255)
                                                                                                                                              cAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATa   >  2:1305641/1‑150 (MQ=255)
                                                                                                                                              cAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATa   <  1:1649827/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATAC  >  CP009273/3657228‑3657520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑