Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,662,660 T→C 100% intergenic (‑112/‑292) yhjA ← / → treF putative cytochrome C peroxidase/cytoplasmic trehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,662,6600TC98.0% 932.0 / 3.0 248intergenic (‑112/‑292)yhjA/treFputative cytochrome C peroxidase/cytoplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (126/116);  total (128/120)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 248 total aligned reads displayed.

CAGGCCGATCGCGGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGATAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTATTTTTATA  >  CP009273/3662513‑3662806
                                                                                                                                                   |                                                                                                                                                  
cagGCCGATCGCGGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAg                                                                                                                                                  >  1:4715110/1‑150 (MQ=255)
 agGCCGATCGCGGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAgg                                                                                                                                                 >  2:1367476/1‑150 (MQ=255)
     cGATCGCGGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGATAGGaatc                                                                                                                                             >  2:2643556/1‑150 (MQ=255)
     cGATCGCGGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGaatc                                                                                                                                             <  2:4923024/150‑1 (MQ=255)
     cGATCGCGGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGaatc                                                                                                                                             >  1:3389902/1‑150 (MQ=255)
         cgcgGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGaatcaatc                                                                                                                                         >  2:3239627/1‑150 (MQ=255)
          gcgGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAatcaatca                                                                                                                                        >  2:1550531/1‑150 (MQ=255)
          gcgGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAatcaatca                                                                                                                                        >  1:2857628/1‑150 (MQ=255)
            ggTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATcaatcaat                                                                                                                                      <  2:2371610/150‑1 (MQ=255)
             gTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAAtt                                                                                                                                     >  2:3627167/1‑150 (MQ=255)
             gTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAAtt                                                                                                                                     <  1:3462450/150‑1 (MQ=255)
              tAAAACGTGAGACCCTCTTCATTATTCTTCCCTCCCGGTGGTGAGTGATTGGGACCCAGAAAATTTTCCAGAAGGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTa                                                                                                                                    <  2:5107885/150‑1 (MQ=255)
               aaTACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTAc                                                                                                                                   >  1:4104978/1‑150 (MQ=255)
                 tACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGAACCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACAt                                                                                                                                 <  2:1918727/150‑1 (MQ=255)
                   cGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGa                                                                                                                               >  1:4698364/1‑150 (MQ=255)
                      gagaCCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAata                                                                                                                            >  1:1332534/1‑150 (MQ=255)
                          ccATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATaaa                                                                                                                        <  2:1464259/150‑1 (MQ=255)
                           cATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAAc                                                                                                                       >  1:1492358/1‑150 (MQ=255)
                            aTTTTCATTATTCTTCACTCAGGGTGGTTAGTTATTGTGACCCATAAAATTTTCCAGAATGCTTCAAACGTCTCTGAGAGACAAAGCCATCATAAAAAACAGTGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACt                                                                                                                      <  1:3583053/150‑1 (MQ=255)
                              tttCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTAt                                                                                                                    >  2:2089509/1‑150 (MQ=255)
                                    tattCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATaa                                                                                                              >  2:1601664/1‑150 (MQ=255)
                                           cccTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGcc                                                                                                       <  1:1415511/150‑1 (MQ=255)
                                             cTCACGGTGGTGTGTTATTGTGACCCAGAATATTTTCCAGAATGCTGAAAACGTCTCTTCGAGACAAAGCCATCATAAAAAACAGGGAGATACGATTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCtg                                                                                                     <  2:2162621/150‑1 (MQ=255)
                                                  ggtggtGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCt                                                                                                >  2:1313785/1‑150 (MQ=255)
                                                     ggtgAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTAtt                                                                                             <  2:4112937/150‑1 (MQ=255)
                                                     ggtgAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTAtt                                                                                             >  1:3001134/1‑150 (MQ=255)
                                                             aTTGTGACCCAGAAAATTTTCCAGAATGCTGCACACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGAt                                                                                     <  1:664718/150‑1 (MQ=255)
                                                             aTTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGAt                                                                                     <  1:2043385/150‑1 (MQ=255)
                                                               tgtgACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCa                                                                                   <  1:3177162/150‑1 (MQ=255)
                                                                 tgACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCCGATCAgg                                                                                 >  2:3448443/1‑150 (MQ=255)
                                                                 tgACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAgg                                                                                 >  1:2257039/1‑150 (MQ=255)
                                                                 tgACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACAATCAATAAGATAGCCTGTGCCTATTACCCAGATCAgg                                                                                 >  1:374554/1‑150 (MQ=255)
                                                                   aCCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGaa                                                                               >  1:3433349/1‑150 (MQ=255)
                                                                     ccAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATg                                                                             <  1:1225667/150‑1 (MQ=255)
                                                                     ccAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATg                                                                             >  2:2518394/1‑150 (MQ=255)
                                                                       aGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGc                                                                           <  2:1120640/150‑1 (MQ=255)
                                                                       aGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGc                                                                           >  2:4791689/1‑150 (MQ=255)
                                                                            aTTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATc                                                                      >  1:820237/1‑150 (MQ=255)
                                                                              tttCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCAt                                                                    >  2:1064580/1‑150 (MQ=255)
                                                                              tttCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCAt                                                                    >  1:1661131/1‑150 (MQ=255)
                                                                                 ccAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGAt                                                                 >  2:4626213/1‑150 (MQ=255)
                                                                                   aGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTg                                                               <  2:4019440/150‑1 (MQ=255)
                                                                                     aaTGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTgcg                                                             >  2:3592050/1‑150 (MQ=255)
                                                                                     aaTGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTgcg                                                             <  1:2471710/150‑1 (MQ=255)
                                                                                      aTGTTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTgcgg                                                            <  1:1081406/150‑1 (MQ=255)
                                                                                       tgctgcAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTgcggc                                                           >  2:1200294/1‑150 (MQ=255)
                                                                                       tgctgcAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTgcggc                                                           >  1:1573180/1‑150 (MQ=255)
                                                                                        gctgcAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTgcggcg                                                          <  2:2757332/150‑1 (MQ=255)
                                                                                         ctgcAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCga                                                         >  1:1883588/1‑150 (MQ=255)
                                                                                         ctgcAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCga                                                         >  1:575693/1‑150 (MQ=255)
                                                                                          tgcAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCgag                                                        >  1:2896747/1‑150 (MQ=255)
                                                                                          tgcAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCgag                                                        >  1:3044112/1‑150 (MQ=255)
                                                                                           gcAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCgaga                                                       >  2:3847538/1‑150 (MQ=255)
                                                                                            cAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGagag                                                      >  1:3997178/1‑150 (MQ=255)
                                                                                               aCGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAg                                                   <  2:1479662/150‑1 (MQ=255)
                                                                                                cGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAgg                                                  <  1:4594802/150‑1 (MQ=255)
                                                                                                cGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAgg                                                  <  1:3783561/150‑1 (MQ=255)
                                                                                                 gTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGa                                                 >  1:1913966/1‑150 (MQ=255)
                                                                                                   ctctGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGAtt                                               <  2:2257039/150‑1 (MQ=255)
                                                                                                   ctctGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGAtt                                               <  2:51605/150‑1 (MQ=255)
                                                                                                    tctGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTg                                              >  2:4250701/1‑150 (MQ=255)
                                                                                                    tctGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTg                                              >  1:2693748/1‑150 (MQ=255)
                                                                                                     ctGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGa                                             >  1:2316597/1‑150 (MQ=255)
                                                                                                      tGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGCTTGaa                                            >  2:182079/1‑150 (MQ=255)
                                                                                                      tGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGaa                                            >  2:3071367/1‑150 (MQ=255)
                                                                                                          agaCAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGtt                                        <  2:971476/150‑1 (MQ=255)
                                                                                                          agaCAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGtt                                        <  1:4834175/150‑1 (MQ=255)
                                                                                                           gaCAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTc                                       >  2:781181/1‑150 (MQ=255)
                                                                                                           gaCAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTc                                       <  2:2857628/150‑1 (MQ=255)
                                                                                                             cAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCtg                                     >  1:1997960/1‑150 (MQ=255)
                                                                                                              aaaGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCtga                                    >  1:2342098/1‑150 (MQ=255)
                                                                                                              aaaGCCATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATAATGATTGAGTCGAGAGCAGGATTGAATGTTCtga                                    >  1:4902021/1‑150 (MQ=255)
                                                                                                                  ccATCATAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCtgaaaat                                <  2:4563014/150‑1 (MQ=255)
                                                                                                                   catcatAAAAAACAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCtgaaaatg                               >  2:2901082/1‑150 (MQ=255)
                                                                                                                      catAAAAAACAGGGAGATATGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCtgaaaatgaaa                            >  1:1023389/1‑150 (MQ=255)
                                                                                                                          aaaaaCAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAaaca                        <  2:605756/150‑1 (MQ=255)
                                                                                                                           aaaaCAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAaacaa                       <  1:92012/150‑1 (MQ=255)
                                                                                                                             aaCAGGTAGATACGCTTTTTGACAGTAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAacaaca                     <  2:4715110/150‑1 (MQ=255)
                                                                                                                             aaCAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAacaaca                     >  2:3384329/1‑150 (MQ=255)
                                                                                                                             aaCAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAacaaca                     >  1:3926204/1‑150 (MQ=255)
                                                                                                                              aCAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAacac                    >  1:4293466/1‑150 (MQ=255)
                                                                                                                              aCAGGGAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAacac                    >  1:529291/1‑150 (MQ=255)
                                                                                                                                tgggAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCa                  >  2:4962721/2‑150 (MQ=255)
                                                                                                                                 gggAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCaa                 >  2:4409476/1‑150 (MQ=255)
                                                                                                                                 gggAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCaa                 <  1:2137467/150‑1 (MQ=255)
                                                                                                                                  ggAGATACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCaaa                <  2:1235869/150‑1 (MQ=255)
                                                                                                                                    agaTACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATc              <  1:1495909/150‑1 (MQ=255)
                                                                                                                                    agaTACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATc              >  2:723379/1‑150 (MQ=255)
                                                                                                                                     gaTACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCg             <  1:3857379/150‑1 (MQ=255)
                                                                                                                                      aTACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCgg            <  1:367079/150‑1 (MQ=255)
                                                                                                                                       tACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGt           <  1:1194040/150‑1 (MQ=255)
                                                                                                                                       tACGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGt           <  1:1067482/150‑1 (MQ=255)
                                                                                                                                        aCGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTa          <  2:5013578/150‑1 (MQ=255)
                                                                                                                                        aCGCTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTa          <  1:4191391/150‑1 (MQ=255)
                                                                                                                                           cTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTAttt       >  1:4376490/1‑150 (MQ=255)
                                                                                                                                           cTTTTTGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTAttt       <  1:3480700/150‑1 (MQ=255)
                                                                                                                                            tttttGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTAtttt      <  1:1313785/150‑1 (MQ=255)
                                                                                                                                            tttttGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTAtttt      <  2:1079371/150‑1 (MQ=255)
                                                                                                                                             ttttGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTAttttt     <  1:1367476/150‑1 (MQ=255)
                                                                                                                                                tGACAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTATTTTtata  >  2:3717300/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGGCCGATCGCGGTAATACGTGAGACCATTTTCATTATTCTTCCCTCACGGTGGTGAGTTATTGTGACCCAGAAAATTTTCCAGAATGCTGCAAACGTCTCTGCGAGACAAAGCCATCATAAAAAACAGGGAGATACGCTTTTTGATAGGAATCAATCAATTACATGAATATAAACTATCAATAAGATAGCCTGTGCCTATTACCCAGATCAGGAATGGCATATCATGATTGCGGCGAGAGCAGGATTGAATGTTCTGAAAATGAAAAACAACACCAAATCGGTATTTTTATA  >  CP009273/3662513‑3662806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑