BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,663,166	T→A	7.1%	F72Y (TTC→TAC)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,663,166	0	T	A	7.1%	825.4 / 40.6	269	F72Y (TTC→TAC)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand): ref base T (124/125); new base A (8/11); total (132/137)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 275 total aligned reads displayed.

TGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGC  >  CP009273/3663017‑3663314

 gAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTc                                                                                                                                                     <  2:3476237/150‑1 (MQ=255)

 gAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTc                                                                                                                                                     >  2:756716/1‑150 (MQ=255)

 gAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTc                                                                                                                                                     <  1:4247512/150‑1 (MQ=255)

      ggACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGa                                                                                                                                                >  2:3609907/1‑150 (MQ=255)

      ggACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGa                                                                                                                                                <  2:5077842/150‑1 (MQ=255)

            gTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTaa                                                                                                                                          >  2:4943896/1‑150 (MQ=255)

             tATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTACCCCGACAGTaaa                                                                                                                                         <  2:3391572/150‑1 (MQ=255)

                     acaaCTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATAATCCCCGACAGTAAAACCTTTcc                                                                                                                                 >  2:2666471/3‑150 (MQ=255)

                       aaCTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCg                                                                                                                               <  2:4246058/150‑1 (MQ=255)

                       aaCTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCg                                                                                                                               >  1:1108010/1‑150 (MQ=255)

                          tGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACt                                                                                                                            <  1:910806/150‑1 (MQ=255)

                           ggATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTg                                                                                                                           >  1:1884930/1‑150 (MQ=255)

                           ggATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTACCCCGACAGTAAAACCTTTCCCGACTg                                                                                                                           <  2:4611334/150‑1 (MQ=255)

                            gatgagatgaTCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTgc                                                                                                                          <  2:2063905/150‑1 (MQ=255)

                                agatgaTCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCAc                                                                                                                      >  1:4964981/1‑150 (MQ=255)

                                agatgaTCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCAc                                                                                                                      >  1:5161418/1‑150 (MQ=255)

                                  atgatCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCt                                                                                                                    <  2:1210706/150‑1 (MQ=255)

                                  atgatCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCt                                                                                                                    >  1:1274547/1‑150 (MQ=255)

                                  atgatCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCCTTCCCGACTGCGCACCt                                                                                                                    <  2:1934228/150‑1 (MQ=255)

                                   tgatCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTa                                                                                                                   <  2:4535789/150‑1 (MQ=255)

                                    gatCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTaa                                                                                                                  <  1:2911319/150‑1 (MQ=255)

                                       cGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAAt                                                                                                               <  2:4389329/150‑1 (MQ=255)

                                        gAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTCAAACCTTTCCCGACTGCGCACCTAACATg                                                                                                              >  2:2940134/1‑150 (MQ=255)

                                        gAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATg                                                                                                              <  2:2997055/150‑1 (MQ=255)

                                           gCAGAACCGTTACCAGTGATGATTGTAGGTCTGCCTGCATCTGATGCACTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCTAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGAc                                                                                                           <  1:3863442/150‑1 (MQ=255)

                                             aGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGAccc                                                                                                         <  1:4692049/150‑1 (MQ=255)

                                             aGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGAccc                                                                                                         <  1:451411/150‑1 (MQ=255)

                                                  cGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTgg                                                                                                    <  1:2223247/150‑1 (MQ=255)

                                                       cccGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATc                                                                                               >  1:1572579/1‑150 (MQ=255)

                                                        ccGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCt                                                                                              <  1:552855/150‑1 (MQ=255)

                                                         cGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCtt                                                                                             >  2:1395813/1‑150 (MQ=255)

                                                          gagaTGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTa                                                                                            >  2:837383/1‑150 (MQ=255)

                                                           agaTGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTaa                                                                                           >  2:4598546/1‑150 (MQ=255)

                                                           agaTGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTaa                                                                                           <  1:3597310/150‑1 (MQ=255)

                                                             atgatTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATc                                                                                         >  1:1794334/1‑150 (MQ=255)

                                                              tgatTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATcc                                                                                        >  2:362393/1‑150 (MQ=255)

                                                                 ttGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCt                                                                                     >  2:2452036/1‑150 (MQ=255)

                                                                  tGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACATTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTa                                                                                    <  1:1355417/150‑1 (MQ=255)

                                                                  tGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTa                                                                                    <  2:5048984/150‑1 (MQ=255)

                                                                   gAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTAc                                                                                   >  2:3282153/1‑150 (MQ=255)

                                                                     aGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCg                                                                                 <  1:1009609/150‑1 (MQ=255)

                                                                      gggCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGt                                                                                <  2:2576277/150‑1 (MQ=255)

                                                                       ggCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTACCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTa                                                                               >  2:2328342/1‑150 (MQ=255)

                                                                          tgcctgccTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAg                                                                            >  1:37997/1‑150 (MQ=255)

                                                                            cctgcctCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTg                                                                          <  2:4518519/150‑1 (MQ=255)

                                                                             ctgcctCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgc                                                                         <  2:3217586/150‑1 (MQ=255)

                                                                              tgcctCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTgcg                                                                        >  2:2278752/1‑150 (MQ=255)

                                                                                catctGATGCGCTGACGCCTGCCGATCGCTATATCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTATCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCTCTACCGTAAAGTGAGcc                                                                      >  2:2777271/3‑150 (MQ=255)

                                                                                 ctctGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCg                                                                     <  2:3908758/150‑1 (MQ=255)

                                                                                 ctctGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCg                                                                     >  1:519804/1‑150 (MQ=255)

                                                                                   ctGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTc                                                                   <  2:1176484/150‑1 (MQ=255)

                                                                                     gATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCAt                                                                 >  1:2118809/1‑150 (MQ=255)

                                                                                      aTGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATc                                                                <  1:3319989/150‑1 (MQ=255)

                                                                                         cgcTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTg                                                             >  1:404146/1‑150 (MQ=255)

                                                                                           cTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGAt                                                           <  2:2338343/150‑1 (MQ=255)

                                                                                               cGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTg                                                       <  1:4161075/150‑1 (MQ=255)

                                                                                                 ccTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGAGATTTTGAc                                                     >  2:3071399/1‑150 (MQ=255)

                                                                                                  cTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACt                                                    >  1:1085345/1‑150 (MQ=255)

                                                                                                   tGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACtt                                                   <  1:3377175/150‑1 (MQ=255)

                                                                                                    gCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTg                                                  >  1:3004693/1‑150 (MQ=255)

                                                                                                    gCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTg                                                  <  1:3439373/150‑1 (MQ=255)

                                                                                                    gCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTg                                                  <  2:1323126/150‑1 (MQ=255)

                                                                                                    gCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTg                                                  <  1:3730400/150‑1 (MQ=255)

                                                                                                     ccGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAGTTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTgc                                                 <  2:3999308/150‑1 (MQ=255)

                                                                                                      cGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTgcg                                                >  1:170291/1‑150 (MQ=255)

                                                                                                      cGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTgcg                                                >  2:1757529/1‑150 (MQ=255)

                                                                                                        aTCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCa                                              <  1:3718911/150‑1 (MQ=255)

                                                                                                             tATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGttt                                         >  2:2126905/1‑150 (MQ=255)

                                                                                                              aTCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttg                                        <  1:1519408/150‑1 (MQ=255)

                                                                                                               tctcGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttgt                                       >  2:2541129/1‑150 (MQ=255)

                                                                                                                ctcGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttgtt                                      <  1:1965322/150‑1 (MQ=255)

                                                                                                                ctcGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttgtt                                      <  2:3961718/150‑1 (MQ=255)

                                                                                                                 tcGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTACCCCGACATTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttgttg                                     <  1:3175486/150‑1 (MQ=255)

                                                                                                                  cGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGa                                    <  1:4420234/150‑1 (MQ=255)

                                                                                                                  cGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGa                                    <  1:4054270/150‑1 (MQ=255)

                                                                                                                   gAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGaa                                   >  2:3832388/1‑150 (MQ=255)

                                                                                                                    aaCTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGaaa                                  <  2:679251/150‑1 (MQ=255)

                                                                                                                       tGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAAcc                               >  2:4153254/1‑150 (MQ=255)

                                                                                                                        gTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCa                              >  2:3126120/1‑150 (MQ=255)

                                                                                                                        gTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCa                              >  2:4705996/1‑150 (MQ=255)

                                                                                                                         ttCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCAc                             >  2:481187/1‑150 (MQ=255)

                                                                                                                         ttCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCAc                             >  2:3713177/1‑150 (MQ=255)

                                                                                                                         ttCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCAc                             >  2:2089735/1‑150 (MQ=255)

                                                                                                                           cGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACtt                           <  2:4995679/150‑1 (MQ=255)

                                                                                                                             aGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAACTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCt                         <  2:1700584/150‑1 (MQ=255)

                                                                                                                              gCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGTGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTg                        <  1:720514/150‑1 (MQ=255)

                                                                                                                              gCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTg                        <  1:818778/150‑1 (MQ=255)

                                                                                                                                aTGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGAGTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGc                      >  1:2414604/1‑150 (MQ=255)

                                                                                                                                 tGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCt                     <  1:2571624/150‑1 (MQ=255)

                                                                                                                                 tGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCt                     >  1:236664/1‑150 (MQ=255)

                                                                                                                                    tCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGcc                  >  2:4447693/1‑150 (MQ=255)

                                                                                                                                     cAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCg                 >  2:674748/1‑150 (MQ=255)

                                                                                                                                     cAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCg                 <  1:3103286/150‑1 (MQ=255)

                                                                                                                                     cAGTCGGCGAAAATTTACCCCGACAGTAAAACCTTTCCCGACAGCGCACCTAAAATGGACCCGCTGGAAATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCg                 >  1:942408/1‑150 (MQ=255)

                                                                                                                                      aGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCgg                <  1:1424998/150‑1 (MQ=255)

                                                                                                                                        tCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAg              <  2:1573448/150‑1 (MQ=255)

                                                                                                                                          ggCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGt            >  2:2233036/1‑150 (MQ=255)

                                                                                                                                           gCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTc           <  1:2145443/150‑1 (MQ=255)

                                                                                                                                           gCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTc           <  1:4680338/150‑1 (MQ=255)

                                                                                                                                                  ttttCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCa    <  2:1495739/150‑1 (MQ=255)

TGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGC  >  CP009273/3663017‑3663314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`