Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,485 T→G 6.4% Y416S (TAC→TCC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4850TG 6.4% 540.4 / 2.8 241Y416S (TAC→TCC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (97/122);  new base G (17/5);  total (114/127)
Fisher's exact test for biased strand distribution p-value = 3.45e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

ATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  CP009273/3721337‑3721634
                                                                                                                                                    |                                                                                                                                                     
aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCATACGGTAGTTGCGGCAACAATTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTa                                                                                                                                                      <  2:3013122/150‑1 (MQ=255)
 tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTCCGGtga                                                                                                                                                     >  1:3646954/1‑148 (MQ=255)
  aaGCGGCATAACGCTGCGCATCTGGGAGGGGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGtcgt                                                                                                                                                    >  2:3890937/1‑147 (MQ=255)
  aaGCGGCAAAACGCTGCGAATCAGGTAGAAGCGAATGTTCTAACAGTAGTTGCGGCAACAATTCAATCAGCGATTTATCTGGATTAGCCGCCATCTGCGCAAACCAAGCTGCGCCCACTTCTCGCCCCACATCCCCCCCAGAACGTTAAt                                                                                                                                                    >  2:146973/1‑150 (MQ=255)
      ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGAATCGAg                                                                                                                                                >  2:193655/1‑150 (MQ=255)
       gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                               <  2:2846862/150‑1 (MQ=255)
        cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgct                                                                                                                                              <  1:3635333/150‑1 (MQ=255)
         aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctg                                                                                                                                             <  1:2099127/150‑1 (MQ=255)
         aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctg                                                                                                                                             <  2:426676/150‑1 (MQ=255)
         aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctg                                                                                                                                             >  2:2924369/1‑150 (MQ=255)
            aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGctgctg                                                                                                                                          <  1:2184400/150‑1 (MQ=255)
                tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                                      <  2:4866439/150‑1 (MQ=255)
                    cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                                                  <  2:2606708/150‑1 (MQ=255)
                     aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                                                 <  1:3851914/150‑1 (MQ=255)
                       cTGGTAGTTGCGACTGTTCTAACTGTAGGTGCGGCAACTATTCAATGCTCGTTGTCTCTGGATTCGCCTCGATCTGCGACAGACTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTATTCGAGCTGCTGACCGCTGatat                                                                                                                               <  2:4191585/150‑1 (MQ=255)
                       cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGatat                                                                                                                               <  1:4397388/150‑1 (MQ=255)
                                  gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCGCCGCCGGTTCGGTGGTCGCGCTGGTGGCCGCTGAGATCCGCCGGCGTc                                                                                                                    >  2:449879/1‑150 (MQ=255)
                                   aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTGATCGAGCTGCTGACCGCTGGTCGccgcccgcgtgt                                                                                                                   >  1:4491072/1‑146 (MQ=255)
                                    cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCGGTCCGCGGGTATCCGCCAGCATCTg                                                                                                                  >  1:1028912/1‑150 (MQ=255)
                                       ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               <  2:4101623/150‑1 (MQ=255)
                                       ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                                                               <  2:1089172/150‑1 (MQ=255)
                                        tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                                              <  1:124116/150‑1 (MQ=255)
                                           aaCGGTAGTTGCGGCAACAAGTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                                                           <  2:2023208/150‑1 (MQ=255)
                                            aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCGGAGATCCGCCAGCAGCTGACGCCAGt                                                                                                          >  2:3904408/1‑150 (MQ=255)
                                              ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTCCGGTCATCGAGCTGCTGTCCGCTGCTATCCGCCCGCGTCTGACGccgggcc                                                                                                        >  2:3708037/1‑145 (MQ=255)
                                                tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCGCCCCCCGTTCGGTGAGCGAGCTGCTGGGCGCGGCTAGCCGCCAGCGGCTGACGCCAGTGCTc                                                                                                      >  1:1105138/1‑150 (MQ=255)
                                                  gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                                   <  1:1584391/150‑1 (MQ=255)
                                                    tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                                  <  1:1404744/150‑1 (MQ=255)
                                                       ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGTGCTGGTGGCCGCTGATAGCCGCCGGCATCTGACGCCAGTGCTCACTAcgc                                                                                               >  1:2952422/1‑150 (MQ=255)
                                                       ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTGAGCGGGCTGCGGACCGCTGCTCTCCGCCCGCCTCTGACGCCCGTCCGCACTCcgc                                                                                               >  1:3906114/1‑150 (MQ=255)
                                                               gtCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                       <  2:4074063/149‑1 (MQ=255)
                                                                tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGGGATCGAGCTGCTGCCCGCTGCTCTCCGCCAGCATGTGACGCCCGTGCTCGCTGCGcgccccgccc                                                                                      >  2:44422/1‑150 (MQ=255)
                                                                   aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                                                   <  1:1139226/150‑1 (MQ=255)
                                                                      aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                                <  1:1688755/150‑1 (MQ=255)
                                                                      aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTTCTCACGACGCGCCCCGCCCCCACTc                                                                                >  2:2153229/1‑150 (MQ=255)
                                                                       gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTCCGGTACTCGAGCTGCTGGCCGCGGAGTTCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGcccccgagca                                                                               >  1:2045754/1‑145 (MQ=255)
                                                                          aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                                                            <  2:2307806/150‑1 (MQ=255)
                                                                            ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCAGCGGGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACGCACTACGCGCCCCGCCCCCACTCCACGTa                                                                          >  1:3597054/1‑150 (MQ=255)
                                                                              ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGGCGGTACTCGTGCTGCTGACCGCTGATTTCCGCCAGCATCTGACGGCAGTCCTCACTACGCGCCCCGCGCCCCCTCaagggagc                                                                        >  2:4952804/1‑144 (MQ=255)
                                                                               tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAaca                                                                       <  1:1023785/150‑1 (MQ=255)
                                                                                ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAaccc                                                                      >  2:2250701/1‑148 (MQ=255)
                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTCATCGTGCTGCTGTCCGGGGATGTCCGCCAGCATCTGGCGCCCGGACTCACTACGCGCCCCGCCCCCCATCAACGTTCCACt                                                                     >  1:2524314/1‑150 (MQ=255)
                                                                                 tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCGGGTATCCGCCCGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCCACGGAACACt                                                                     >  1:2661472/1‑150 (MQ=255)
                                                                                  ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                                                    <  1:4859223/150‑1 (MQ=255)
                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                                   <  2:3613566/150‑1 (MQ=255)
                                                                                   gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                                   <  1:405477/150‑1 (MQ=255)
                                                                                    aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTCCTCACTACGCGCCCCGCCCCCAATCACCGTACCACTTTg                                                                  >  1:1951627/1‑150 (MQ=255)
                                                                                     ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                                 <  1:2898437/150‑1 (MQ=255)
                                                                                       cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                                               <  1:3013792/150‑1 (MQ=255)
                                                                                        gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGCGCTGCTGTCCGCTGTTATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGTCACACGTGGCGGt                                                              >  2:1798323/1‑150 (MQ=255)
                                                                                          cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                            <  2:4998067/150‑1 (MQ=255)
                                                                                          cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                            <  2:332317/150‑1 (MQ=255)
                                                                                           gctaTCTGCTCCAGCCTTGCTGCGCCGAGTGCTTGACCCTCATCCACCCCCGTACGGTAATCGAGCTGCTTACCGCTGATATCCGCCATCTTATGACGCCAGTTCTCAATACGAGCCACGCCCCCATTCAACGTAACACTTTGCGTTTTa                                                           <  2:1605813/147‑1 (MQ=255)
                                                                                             gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGTCGGTACTCGAGCTGCTGACCGCTGATGGCCGCCAGCATCTGACGCCAGTCCTCACTCCGCGCCCCGCCCCCAAGCAGCGTTTCACTTTGCGGttatct                                                         >  1:900560/1‑146 (MQ=255)
                                                                                             gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                         <  1:581548/150‑1 (MQ=255)
                                                                                             gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCGACGTAACACGTTGCGGTTTAAt                                                         >  2:3215633/1‑150 (MQ=255)
                                                                                              aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                                        <  1:4595973/150‑1 (MQ=255)
                                                                                               tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                       <  2:4723609/150‑1 (MQ=255)
                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                      <  2:2557736/150‑1 (MQ=255)
                                                                                                cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                      <  2:85077/150‑1 (MQ=255)
                                                                                                 tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTTTTACCg                                                     >  1:1821658/1‑150 (MQ=255)
                                                                                                  gcgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGc                                                    <  1:3350457/150‑1 (MQ=255)
                                                                                                  gcgaCAGCCTTTCTGCGCACATTGCTGGCCCCACATCCCACCCCGTACTGTAATCGAGCTGCTGACCGCTTATATCCTCAAGCTTGTTACTACTTTACTCAGAAAGCGCCCATCCCCCAATCAACGTAACACTTTGCGGTTTTATACAGc                                                    <  2:268944/150‑1 (MQ=255)
                                                                                                   cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                                                   <  1:2646177/150‑1 (MQ=255)
                                                                                                   cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGCTCTCCGCCAGCATCTGCCGCGAGTACTCACTACGCGCCCCGCCCCCAAGCGACGTAACACTTTGCGGTTTAAGACCGCa                                                   >  2:318381/1‑150 (MQ=255)
                                                                                                    gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                                  <  1:1178501/150‑1 (MQ=255)
                                                                                                     ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                                 <  1:2301708/150‑1 (MQ=255)
                                                                                                     ccAGCCTTGCTGCGCCCAGGGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                                 <  2:3423302/150‑1 (MQ=255)
                                                                                                      cAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTCATCGAGCTGCTGACCGGTGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                                                >  1:3444271/1‑150 (MQ=255)
                                                                                                       aGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGTAATCGAGCTGCTGACCGCTGTTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa                                               >  1:3193358/1‑150 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATGGAGCTGCTGTCCGCTTTTATCCGCCCGCATCTGACGCCAGTACTCAATACGCGCCCCGCCCCCACTCCACGGGACGCTTTGCGGTGTAAGACCGCCGGCATg                                             >  2:5139813/1‑150 (MQ=255)
                                                                                                         ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg                                             <  1:195366/150‑1 (MQ=255)
                                                                                                           ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCCGTACTCACTCCGCGCCCCGCCCCCAATCCACGTAACCCTTTGCGGTTTATTACCGCAGGCATGCa                                           >  1:4533093/1‑150 (MQ=255)
                                                                                                           ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGTCCGCTGTTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGGTAATACCGCAGGCATGCa                                           >  1:4308659/1‑150 (MQ=255)
                                                                                                                gcgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCGGCTGACCGCTGATATCCGCCAGCATCTGACGCCGGTACTCACTACGCGCCCCGCCCCCACTCCCCGGAACACGTTGCGGGGTAATGCCGCAGGCATGCACGACa                                      >  2:1315698/1‑150 (MQ=255)
                                                                                                                 cgcCCAGTGCTGGCCCCACAT‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                                    <  2:3756185/150‑1 (MQ=255)
                                                                                                                  gcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                                    <  2:3964097/150‑1 (MQ=255)
                                                                                                                     cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                                 >  2:945660/1‑150 (MQ=255)
                                                                                                                     cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                                 <  1:4727829/150‑1 (MQ=255)
                                                                                                                         gCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa                             >  2:4574794/1‑150 (MQ=255)
                                                                                                                            ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                          >  1:3881470/1‑150 (MQ=255)
                                                                                                                            ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                          <  1:1395140/150‑1 (MQ=255)
                                                                                                                             gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                         >  2:2087763/1‑150 (MQ=255)
                                                                                                                               cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                       >  1:1699862/1‑150 (MQ=255)
                                                                                                                                   caTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg                   >  2:2744358/1‑150 (MQ=255)
                                                                                                                                   caTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg                   >  1:3250072/1‑150 (MQ=255)
                                                                                                                                      ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt                <  2:2500329/150‑1 (MQ=255)
                                                                                                                                       cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa               <  1:4952804/150‑1 (MQ=255)
                                                                                                                                        ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              >  1:1628783/1‑150 (MQ=255)
                                                                                                                                        ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              >  1:271791/1‑150 (MQ=255)
                                                                                                                                            ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa          <  1:4735013/150‑1 (MQ=255)
                                                                                                                                            ccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCa          <  1:449879/150‑1 (MQ=255)
                                                                                                                                              gTACGGTAATCGAGCTGCTGACAGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg        <  2:3143959/150‑1 (MQ=255)
                                                                                                                                               tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc       >  1:2763101/1‑150 (MQ=255)
                                                                                                                                               tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc       >  1:4151304/1‑150 (MQ=255)
                                                                                                                                               tACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGc       >  2:4345500/1‑150 (MQ=255)
                                                                                                                                                aCGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCCGCGCATAACCCACGcc      >  2:3465191/1‑150 (MQ=255)
                                                                                                                                                aCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGcc      >  1:3106041/1‑150 (MQ=255)
                                                                                                                                                 cGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACCCTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCt     <  2:4673087/150‑1 (MQ=255)
                                                                                                                                                    tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTcc  <  1:5025035/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  CP009273/3721337‑3721634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑