BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,723,822	C→A	11.2%	D102Y (GAT→TAT)	xylA ←	D‑xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,723,822	0	C	A	11.2%	1003.7 / 65.7	314	D102Y (GAT→TAT)	xylA	D‑xylose isomerase
Reads supporting (aligned to +/- strand): ref base C (124/154); new base A (17/19); total (141/173)
Fisher's exact test for biased strand distribution p-value = 8.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

GCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAG  >  CP009273/3723673‑3723970

   gTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTg                                                                                                                                                   >  1:425941/1‑150 (MQ=255)

   gTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTg                                                                                                                                                   >  2:829305/1‑150 (MQ=255)

   gTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTg                                                                                                                                                   <  2:4107986/150‑1 (MQ=255)

    tAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTgg                                                                                                                                                  >  2:745692/1‑150 (MQ=255)

        gAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGc                                                                                                                                              <  1:7969/150‑1 (MQ=255)

             tttGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATaa                                                                                                                                         <  2:123793/150‑1 (MQ=255)

               tGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATaaaa                                                                                                                                       <  2:2769090/150‑1 (MQ=255)

                  aaaGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATgg                                                                                                                                    <  1:1030497/150‑1 (MQ=255)

                  aaaGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATgg                                                                                                                                    >  2:1346676/1‑150 (MQ=255)

                      cAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGcaca                                                                                                                                <  2:1746006/150‑1 (MQ=255)

                       aGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACAt                                                                                                                               >  1:3661759/1‑150 (MQ=255)

                           ggCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTaa                                                                                                                           <  1:555881/150‑1 (MQ=255)

                             ccGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACt                                                                                                                         <  2:3503611/150‑1 (MQ=255)

                               gTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTtg                                                                                                                       <  2:2401874/150‑1 (MQ=255)

                               gTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTtg                                                                                                                       >  1:1946336/1‑150 (MQ=255)

                                 tCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTtgtg                                                                                                                     >  1:1341860/1‑150 (MQ=255)

                                 tCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTtgtg                                                                                                                     >  1:3922118/1‑150 (MQ=255)

                                 tCCACACAGCAGCTTCACGCCGATCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTTACGACGCTCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTtgtg                                                                                                                     <  1:3353769/150‑1 (MQ=255)

                                  ccccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTgg                                                                                                                    >  2:4472039/1‑150 (MQ=255)

                                    ccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGaa                                                                                                                  >  1:3415404/1‑150 (MQ=255)

                                    ccACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCAAATGTAACGTGTGGaa                                                                                                                  >  2:2679773/1‑150 (MQ=255)

                                     cacaGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGaaa                                                                                                                 >  1:873058/1‑150 (MQ=255)

                                      acaGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGaaaa                                                                                                                <  1:5023643/150‑1 (MQ=255)

                                      acaGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGaaaa                                                                                                                <  1:2127881/150‑1 (MQ=255)

                                         gcagcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAACATGGCACATGTAACTTGTGGAAAAACt                                                                                                             <  1:4384/150‑1 (MQ=255)

                                         gcagcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACt                                                                                                             <  1:4517902/150‑1 (MQ=255)

                                            gcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCaa                                                                                                          <  1:773882/150‑1 (MQ=255)

                                                  cgccgcTCTCTTCGTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGa                                                                                                    <  2:4276150/150‑1 (MQ=255)

                                                    ccgcTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACa                                                                                                  >  1:31335/1‑150 (MQ=255)

                                                    ccgcTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACa                                                                                                  >  2:3232793/1‑150 (MQ=255)

                                                     cgcTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACAt                                                                                                 >  1:4900277/1‑150 (MQ=255)

                                                       ctctctTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCt                                                                                               <  1:5118941/150‑1 (MQ=255)

                                                       ctctctTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCACTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCt                                                                                               <  1:2379404/150‑1 (MQ=255)

                                                        tctctTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTg                                                                                              <  1:4462481/150‑1 (MQ=255)

                                                         ctctTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGc                                                                                             >  2:1011512/1‑150 (MQ=255)

                                                          tcttctTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCt                                                                                            <  2:2068656/150‑1 (MQ=255)

                                                             tcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTa                                                                                         <  2:2892258/150‑1 (MQ=255)

                                                             tcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTa                                                                                         <  1:282373/150‑1 (MQ=255)

                                                              cttgcttgCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTAc                                                                                        <  2:4703622/150‑1 (MQ=255)

                                                               ttgcttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACg                                                                                       <  1:3257398/150‑1 (MQ=255)

                                                               ttgcttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACg                                                                                       >  1:5083813/1‑150 (MQ=255)

                                                               ttgcttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACg                                                                                       >  2:1565668/1‑150 (MQ=255)

                                                               ttgcttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACg                                                                                       <  1:257816/150‑1 (MQ=255)

                                                               ttgcttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACg                                                                                       <  1:3941258/150‑1 (MQ=255)

                                                                  cttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCtt                                                                                    >  2:2463054/1‑150 (MQ=255)

                                                                  cttgcCTGCCACGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCtt                                                                                    <  1:1407958/150‑1 (MQ=255)

                                                                   ttgcCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTc                                                                                   <  2:1941855/150‑1 (MQ=255)

                                                                    tgcctgccAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCg                                                                                  <  1:1209467/150‑1 (MQ=255)

                                                                    tgcctgccAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCg                                                                                  >  2:3071342/1‑150 (MQ=255)

                                                                       ctgccAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCa                                                                               <  1:2711029/150‑1 (MQ=255)

                                                                              gACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAg                                                                        <  2:232170/150‑1 (MQ=255)

                                                                               aCATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGt                                                                       <  2:341535/150‑1 (MQ=255)

                                                                                cATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTg                                                                      >  2:3978211/1‑150 (MQ=255)

                                                                                 aTCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGc                                                                     <  2:3722951/150‑1 (MQ=255)

                                                                                 aTCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGc                                                                     >  2:391668/1‑150 (MQ=255)

                                                                                   cAACCATTTGCGCAAAAGTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCt                                                                   <  2:438195/150‑1 (MQ=255)

                                                                                       cATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCAcc                                                               <  1:4564793/150‑1 (MQ=255)

                                                                                          ttGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAgg                                                            <  2:111776/150‑1 (MQ=255)

                                                                                            gcgcAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAACTGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGgct                                                          <  2:3673527/150‑1 (MQ=255)

                                                                                            gcgcAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGgct                                                          <  2:4526409/150‑1 (MQ=255)

                                                                                            gcgcAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGgct                                                          <  1:721744/150‑1 (MQ=255)

                                                                                              gcAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGgctgc                                                        >  2:1813165/1‑150 (MQ=255)

                                                                                                 aaaTTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGACACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCtgctgc                                                     <  2:3836760/150‑1 (MQ=255)

                                                                                                  aaTTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGcc                                                    >  1:4596513/1‑150 (MQ=255)

                                                                                                  aaTTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGcc                                                    <  2:2972841/150‑1 (MQ=255)

                                                                                                        tGATGTACTCTTTTACCGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACGTGTGGAAAAACTCAACAGCGACATCTGCTTTACGCTTCGCCAACGCCAGGGCCTCACCAGGCTGCTGCCCCGGAc                                              <  2:5090954/150‑1 (MQ=255)

                                                                                                        tGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGAc                                              >  1:2227172/1‑150 (MQ=255)

                                                                                                        tGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGAc                                              <  2:621460/150‑1 (MQ=255)

                                                                                                          aTGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGa                                            <  1:3312335/150‑1 (MQ=255)

                                                                                                               ctctTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTaaa                                       >  2:3742629/1‑150 (MQ=255)

                                                                                                               ctctTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTaaa                                       >  1:2634357/1‑150 (MQ=255)

                                                                                                                tctTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAAc                                      <  1:4943874/150‑1 (MQ=255)

                                                                                                                 ctTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACg                                     >  2:496468/1‑150 (MQ=255)

                                                                                                                  ttttAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGc                                    >  1:1854506/1‑150 (MQ=255)

                                                                                                                     tAACGACGCGCCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGcccc                                 >  1:647378/1‑150 (MQ=255)

                                                                                                                       acgacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCca                               <  1:4217551/150‑1 (MQ=255)

                                                                                                                       acgacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGGGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCca                               <  1:2048482/150‑1 (MQ=255)

                                                                                                                         gacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCcaca                             >  1:1921773/1‑150 (MQ=255)

                                                                                                                           tgcgcCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACAcc                           >  1:1610472/2‑150 (MQ=255)

                                                                                                                            gcgcCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGTGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCa                          >  2:1527992/1‑150 (MQ=255)

                                                                                                                            gcgcCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCa                          >  2:921796/1‑150 (MQ=255)

                                                                                                                              gcCCTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCaaa                        <  1:1491197/150‑1 (MQ=255)

                                                                                                                                ccTCAGGGGAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACa                      >  1:3795419/1‑150 (MQ=255)

                                                                                                                                 cTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACat                     <  1:3871933/150‑1 (MQ=255)

                                                                                                                                   cAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACatat                   <  2:4646037/150‑1 (MQ=255)

                                                                                                                                     ggggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATcc                 >  2:2990636/1‑150 (MQ=255)

                                                                                                                                     ggggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATcc                 <  1:1888999/150‑1 (MQ=255)

                                                                                                                                      gtgAAACATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCg                <  2:5067316/148‑1 (MQ=255)

                                                                                                                                       tgAAACATCCACATCGTGGAAGCAATAATATGGCACATTTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGc               <  2:2632378/149‑1 (MQ=255)

                                                                                                                                         aaaCATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGccc             <  2:2198194/150‑1 (MQ=255)

                                                                                                                                         aaaCATCCACATAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGccc             >  2:1997693/1‑150 (MQ=255)

                                                                                                                                          aaCATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGTTTAAACGCCCCCACACCAAACATATCCGcccc            <  1:1521080/150‑1 (MQ=255)

                                                                                                                                            cATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGt          <  2:31566/150‑1 (MQ=255)

                                                                                                                                                cacaTCGTTGAAGCAATAAAATGGCTCATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCATCTCCAGTGCCTCACAAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTcct      <  2:969150/150‑2 (MQ=255)

                                                                                                                                                cacaTCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTcct      <  2:905280/150‑2 (MQ=255)

                                                                                                                                                 acaTAGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCATGCTGCTGCAACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCcag     >  2:1918708/1‑150 (MQ=255)

                                                                                                                                                   aTCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCcagca   >  2:4891958/1‑150 (MQ=255)

                                                                                                                                                   aTCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCcagca   <  1:4558108/150‑1 (MQ=255)

                                                                                                                                                    tCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCcagcag  <  1:90887/150‑1 (MQ=255)

                                                                                                                                                    tCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCcagcag  <  1:3433167/150‑1 (MQ=255)

GCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAG  >  CP009273/3723673‑3723970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`