BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,761,964	G→T	6.7%	R143L (CGA→CTA)	yibG →	TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,761,964	0	G	T	6.7%	799.1 / 21.9	223	R143L (CGA→CTA)	yibG	TPR‑like repeat protein
Reads supporting (aligned to +/- strand): ref base G (127/81); new base T (5/10); total (132/91)
Fisher's exact test for biased strand distribution p-value = 5.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 226 total aligned reads displayed.

GTTTAATTATGGGGTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAGGCTCCGA  >  CP009273/3761815‑3762113

 tttAATTATGGGGTCTTTTTATCAGATACAAATCAAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGa                                                                                                                                                      <  1:3276745/150‑1 (MQ=255)

  ttAATTATGGGGTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGaa                                                                                                                                                     <  2:1182053/150‑1 (MQ=255)

  ttAATTATGGGGTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGaa                                                                                                                                                     <  2:2554034/150‑1 (MQ=255)

        aTGGGGTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATa                                                                                                                                               <  1:2909885/150‑1 (MQ=255)

        aTGGGGTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATa                                                                                                                                               <  2:4556721/150‑1 (MQ=255)

         tGGGGTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAg                                                                                                                                              >  2:4453100/1‑150 (MQ=255)

          ggggTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAga                                                                                                                                             >  1:1019585/1‑150 (MQ=255)

           gggTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAgaa                                                                                                                                            <  1:1221947/150‑1 (MQ=255)

             gTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAgaagg                                                                                                                                          <  2:3413961/150‑1 (MQ=255)

              tCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCTAATGGATAgaagga                                                                                                                                         <  1:2052778/150‑1 (MQ=255)

                       cAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAgg                                                                                                                                >  2:4166369/1‑150 (MQ=255)

                        tgATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACGTCAAGAGCTCAAAAAGCTAACGCGAATGGATAGAAGGACGAATAAGga                                                                                                                               >  2:4632744/2‑150 (MQ=255)

                        aGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGga                                                                                                                               <  2:2657672/150‑1 (MQ=255)

                          aTACAAATCCAACATTATCAGAATATTATAGTTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGgaga                                                                                                                             >  2:2111846/1‑150 (MQ=255)

                          aTACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCTACTGGATAGAAGGAAGAATAAGgaga                                                                                                                             >  2:341782/1‑150 (MQ=255)

                          aTACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGgaga                                                                                                                             <  1:4372657/150‑1 (MQ=255)

                          aTACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGgaga                                                                                                                             <  2:505162/150‑1 (MQ=255)

                           tACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGaa                                                                                                                            >  1:4529616/1‑150 (MQ=255)

                           tACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGaa                                                                                                                            >  1:2489752/1‑150 (MQ=255)

                           tACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGaa                                                                                                                            >  1:2489618/1‑150 (MQ=255)

                           tACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGaa                                                                                                                            >  2:1140400/1‑150 (MQ=255)

                           tACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGaa                                                                                                                            <  2:2472296/150‑1 (MQ=255)

                            aCAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAAt                                                                                                                           >  2:4254979/1‑150 (MQ=255)

                               aaTCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCg                                                                                                                        >  1:2133317/1‑150 (MQ=255)

                                 tCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTa                                                                                                                      >  1:668850/1‑150 (MQ=255)

                                   cAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAAGGGATAGAAGGAAGAATAAGGAGAATCCGTaaa                                                                                                                    <  2:1671921/150‑1 (MQ=255)

                                    aaCATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTaaaa                                                                                                                   <  1:554292/150‑1 (MQ=255)

                                    aaCATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTaaaa                                                                                                                   >  1:4744080/1‑150 (MQ=255)

                                     aCATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAg                                                                                                                  <  1:1962950/150‑1 (MQ=255)

                                        ttATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCTAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCaa                                                                                                               >  1:4262256/1‑150 (MQ=255)

                                        ttATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCaa                                                                                                               >  1:4129169/1‑150 (MQ=255)

                                         tATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAAt                                                                                                              >  1:2218683/1‑150 (MQ=255)

                                            cAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGt                                                                                                           <  1:2252231/150‑1 (MQ=255)

                                            cAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGt                                                                                                           >  1:3890377/1‑150 (MQ=255)

                                                   ttatAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAg                                                                                                    <  1:3451604/150‑1 (MQ=255)

                                                    tataATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGa                                                                                                   <  1:4200383/150‑1 (MQ=255)

                                                    tataATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGa                                                                                                   >  1:5149900/1‑150 (MQ=255)

                                                    aataATTATAATAGAGATATATATAGGATGGAAAAAGCTGTAAATAACGGTAATAATGATGCAAAGATAAAACAACAAGAGCTCAAAAATATAAAGAGAATGGATAGAAGGAAGAAAAACAAGAATCCGTAAAAGCAATAGATCATCAGa                                                                                                   >  2:826438/2‑150 (MQ=255)

                                                       aaTTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTg                                                                                                >  1:5129303/1‑150 (MQ=255)

                                                       aaTTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTg                                                                                                >  1:3558932/1‑150 (MQ=255)

                                                       aaTTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTg                                                                                                >  1:1175584/1‑150 (MQ=255)

                                                        aTTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGc                                                                                               <  1:3378619/150‑1 (MQ=255)

                                                           tGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCgc                                                                                            >  1:93694/1‑150 (MQ=255)

                                                              aTAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCgcagc                                                                                         >  1:5007864/1‑150 (MQ=255)

                                                                agagCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGcagcag                                                                                       >  1:1569622/1‑150 (MQ=255)

                                                                agagCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGcagcag                                                                                       >  2:3494916/1‑150 (MQ=255)

                                                                 gagCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGACTCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCagt                                                                                      <  1:3157078/150‑1 (MQ=255)

                                                                    cTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCagtagt                                                                                   >  1:161936/1‑150 (MQ=255)

                                                                     tatttattGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAgtagta                                                                                  >  2:4009036/1‑150 (MQ=255)

                                                                     tatttattGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAgtagta                                                                                  <  1:1649343/150‑1 (MQ=255)

                                                                      atttattGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCACAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTat                                                                                 <  2:3374494/150‑1 (MQ=255)

                                                                      atttattGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTat                                                                                 <  1:1380531/150‑1 (MQ=255)

                                                                          attGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTatgatg                                                                             >  2:1824833/1‑150 (MQ=255)

                                                                            tggatggaATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTatgatgat                                                                           >  1:82591/1‑150 (MQ=255)

                                                                             ggatggaATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCTAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTatgatgatg                                                                          >  2:4476610/1‑150 (MQ=255)

                                                                             ggatggaATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTatgatgatg                                                                          >  2:1348464/1‑150 (MQ=255)

                                                                               atggaATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGaa                                                                        >  2:1878718/1‑150 (MQ=255)

                                                                                   aaTTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAg                                                                    >  2:1917603/1‑150 (MQ=255)

                                                                                    aTTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAgg                                                                   <  1:4685945/150‑1 (MQ=255)

                                                                                       cGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCt                                                                >  2:2753233/1‑150 (MQ=255)

                                                                                       cGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCt                                                                >  1:3891030/1‑150 (MQ=255)

                                                                                        gCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCTAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTg                                                               <  2:4530947/150‑1 (MQ=255)

                                                                                         cTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGANTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGt                                                              <  1:2681499/150‑1 (MQ=255)

                                                                                            tAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCGATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACt                                                           <  2:3719552/150‑1 (MQ=255)

                                                                                            tAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACt                                                           <  2:1323866/150‑1 (MQ=255)

                                                                                            tAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACt                                                           <  2:2233496/150‑1 (MQ=255)

                                                                                                tAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATaa                                                       >  1:5098143/1‑150 (MQ=255)

                                                                                                  aCGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACt                                                     >  2:2163765/1‑150 (MQ=255)

                                                                                                   cGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACta                                                    >  2:3264889/1‑150 (MQ=255)

                                                                                                    ggTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACtat                                                   <  1:2837926/150‑1 (MQ=255)

                                                                                                    ggTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACtat                                                   <  2:2184402/150‑1 (MQ=255)

                                                                                                     gTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACtatt                                                  >  2:4774285/1‑150 (MQ=255)

                                                                                                      tAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACtatta                                                 >  1:147415/1‑150 (MQ=255)

                                                                                                               tGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACa                                        >  1:1056692/1‑150 (MQ=255)

                                                                                                                  aaaGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTa                                     >  1:746383/1‑150 (MQ=255)

                                                                                                                    agagCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAAc                                   >  1:471890/1‑150 (MQ=255)

                                                                                                                      agCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCg                                 >  2:3156002/1‑150 (MQ=255)

                                                                                                                       gCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCgc                                >  2:2835683/1‑150 (MQ=255)

                                                                                                                        cAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCgca                               >  2:3395787/1‑150 (MQ=255)

                                                                                                                        cAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCgca                               <  2:1012090/150‑1 (MQ=255)

                                                                                                                          aaCTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCgcagc                             >  1:2337987/1‑150 (MQ=255)

                                                                                                                           aCTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCgcagca                            <  1:3494916/150‑1 (MQ=255)

                                                                                                                              tCAAGATCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAggg                         <  1:3119350/150‑1 (MQ=255)

                                                                                                                               cAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCTCAGCAgggg                        >  2:5141656/1‑150 (MQ=255)

                                                                                                                                aaGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGc                       >  1:62959/1‑150 (MQ=255)

                                                                                                                                    gCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGta                   <  1:1893514/150‑1 (MQ=255)

                                                                                                                                     cTCAAAAAGCTAAAGAGAATGGATATAAGGAAGAATAAGTAGAATCCGTAAAAGAAATATTTCATCAGACTGCCTCAGAAGTAGTATGATGATTAATCAGGGATGTAATATAAATATTATCGGTAACATTAACCGCAGAAGGGTCTGtat                  >  2:439624/1‑150 (MQ=255)

                                                                                                                                         aaaaGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAaca              <  1:3929865/150‑1 (MQ=255)

                                                                                                                                           aaGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACt            <  1:1871378/150‑1 (MQ=255)

                                                                                                                                            aGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTc           <  1:4451091/150‑1 (MQ=255)

                                                                                                                                             gCTAAAGCTAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCa          <  2:2128881/150‑1 (MQ=255)

                                                                                                                                             gCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCa          >  2:5047270/1‑150 (MQ=255)

                                                                                                                                             gCTAAAGAGAATGTATAGAAGGAAGAATAAGCAGAATCCTAAAAACCAATAGTACATCAGACAGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACAATTATAGGTAAAATTAACCTCAGCAGGGGCGGTATAacacaaa          >  2:2205470/1‑147 (MQ=255)

                                                                                                                                              cTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAg         >  2:864418/1‑150 (MQ=255)

                                                                                                                                              cTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAg         >  2:927037/1‑150 (MQ=255)

                                                                                                                                               tAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAgg        <  1:916162/150‑1 (MQ=255)

                                                                                                                                               tAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAgg        >  1:695478/1‑150 (MQ=255)

                                                                                                                                                 aaGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAGGCt      >  1:1773842/1‑150 (MQ=255)

                                                                                                                                                   gCGAATGGATAGAAGGAAGAATATGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAGGCTcc    <  1:3248574/150‑1 (MQ=255)

                                                                                                                                                     gAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAGGCTCCGa  >  2:3852223/1‑150 (MQ=255)

GTTTAATTATGGGGTCTTTTTATCAGATACAAATCCAACATTATCAGAATATTATAATTTGAATAGAGCTATTTATTGGATGGAATTCGCTGTAAATAACGGTAATATTGATGCAAAGAGCAAACTTCAAGAGCTCAAAAAGCTAAAGCGAATGGATAGAAGGAAGAATAAGGAGAATCCGTAAAAGCAATAGTTCATCAGACTGCCGCAGCAGTAGTATGATGATGAATCAGGGCTGTACTATAACTATTATCGGTAACATTAACCGCAGCAGGGGCGGTATAACACTCAGGCTCCGA  >  CP009273/3761815‑3762113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`