Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,767,000 G→T 100% D454Y (GAC→TAC)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,767,0000GT97.9% 1076.9 / 9.6 335D454Y (GAC→TAC) mtlAfused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (170/158);  total (173/162)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 339 total aligned reads displayed.

ATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTCCTC  >  CP009273/3766851‑3767149
                                                                                                                                                     |                                                                                                                                                     
aTGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCg                                                                                                                                                       >  2:3390121/1‑150 (MQ=255)
  gCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTAc                                                                                                                                                     <  2:1897301/150‑1 (MQ=255)
   cAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACa                                                                                                                                                    <  1:4032693/150‑1 (MQ=255)
     ggTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGc                                                                                                                                                  >  2:3606114/1‑150 (MQ=255)
        cTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGc                                                                                                                                               >  2:3819638/1‑150 (MQ=255)
        cTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGc                                                                                                                                               <  1:4399251/150‑1 (MQ=255)
         tGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGcc                                                                                                                                              >  1:319801/1‑150 (MQ=255)
         tGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGcc                                                                                                                                              >  2:4941556/1‑150 (MQ=255)
         tGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGcc                                                                                                                                              >  2:3933120/1‑150 (MQ=255)
          gTCGGAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCTCCAGATGTGGACATCGTCATAACTCACCGTGACATGAACGAACGCGCTATGCGCCAGGTTCCGCAGGCTCAGCATATTTCGCTGTCCAACTTCCTCTACAGCGGCCt                                                                                                                                             <  1:2066767/150‑1 (MQ=255)
            cGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGt                                                                                                                                           <  2:3681321/150‑1 (MQ=255)
            cGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGt                                                                                                                                           <  1:2239814/150‑1 (MQ=255)
            cGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGt                                                                                                                                           >  1:1946411/1‑150 (MQ=255)
                  tttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCa                                                                                                                                     <  2:1852525/150‑1 (MQ=255)
                  tttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCa                                                                                                                                     >  2:2169891/1‑150 (MQ=255)
                       gTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTg                                                                                                                                <  1:3579918/150‑1 (MQ=255)
                        ttACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGa                                                                                                                               >  1:4001617/1‑150 (MQ=255)
                          aCTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGAcc                                                                                                                             <  1:1679353/150‑1 (MQ=255)
                               cAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACg                                                                                                                        >  1:203841/1‑150 (MQ=255)
                                aGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGt                                                                                                                       <  2:579095/150‑1 (MQ=255)
                                 gcgcGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTc                                                                                                                      <  1:4933471/150‑1 (MQ=255)
                                  cgcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCt                                                                                                                     >  1:74854/1‑150 (MQ=255)
                                  cgcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCt                                                                                                                     >  1:3547508/1‑150 (MQ=255)
                                     gATAAACAACCTGCCGCCAGATGTGTACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGt                                                                                                                  >  2:714538/1‑150 (MQ=255)
                                      aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGtt                                                                                                                 <  2:2571467/150‑1 (MQ=255)
                                         aacaacCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtgct                                                                                                              >  2:1588914/1‑150 (MQ=255)
                                         aacaacCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtgct                                                                                                              <  1:3672765/150‑1 (MQ=255)
                                               cTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCaa                                                                                                        <  2:422786/150‑1 (MQ=255)
                                                   cgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGcc                                                                                                    <  2:303236/150‑1 (MQ=255)
                                                   cgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGcc                                                                                                    >  1:798580/1‑150 (MQ=255)
                                                   cgccAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGcc                                                                                                    >  2:3256392/1‑150 (MQ=255)
                                                      cAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCcaca                                                                                                 >  1:1557187/1‑150 (MQ=255)
                                                      cAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCcaca                                                                                                 <  2:2452313/150‑1 (MQ=255)
                                                       aGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCacac                                                                                                >  2:2428491/1‑150 (MQ=255)
                                                       aGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCACCCTGACCGAACGTCTGGTTGCTGCCCAACGCCacac                                                                                                >  2:4258294/1‑150 (MQ=255)
                                                        gATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACg                                                                                               >  2:1440400/1‑150 (MQ=255)
                                                         aTGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg                                                                                              >  1:4833910/1‑150 (MQ=255)
                                                            tgGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCaa                                                                                           <  2:4315411/150‑1 (MQ=255)
                                                             ggACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCaaa                                                                                          <  2:1499357/150‑1 (MQ=255)
                                                               aCCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACg                                                                                        <  1:2891785/150‑1 (MQ=255)
                                                                 cTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGaa                                                                                      <  2:3544271/150‑1 (MQ=255)
                                                                  tCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAag                                                                                     <  1:2422991/150‑1 (MQ=255)
                                                                  tCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAag                                                                                     <  2:135910/150‑1 (MQ=255)
                                                                  tCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAag                                                                                     <  1:3316541/150‑1 (MQ=255)
                                                                  tCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAag                                                                                     <  2:1961869/150‑1 (MQ=255)
                                                                   cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga                                                                                    >  2:4658947/1‑150 (MQ=255)
                                                                   cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga                                                                                    >  2:371190/1‑150 (MQ=255)
                                                                   cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga                                                                                    >  2:2089043/1‑150 (MQ=255)
                                                                     tcatcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga                                                                                  <  2:1143215/150‑1 (MQ=255)
                                                                       atcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaaa                                                                                >  2:199991/1‑150 (MQ=255)
                                                                           ctcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTaa                                                                            <  2:1038999/150‑1 (MQ=255)
                                                                            tcacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTaaa                                                                           >  2:2923140/1‑150 (MQ=255)
                                                                             cacCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCGGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTaaaa                                                                          >  1:1821553/1‑150 (MQ=255)
                                                                              acCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAg                                                                         <  2:4319861/150‑1 (MQ=255)
                                                                                cGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGAc                                                                       >  1:3504262/1‑150 (MQ=255)
                                                                                   gacctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc                                                                    <  1:3842912/150‑1 (MQ=255)
                                                                                      ctgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTg                                                                 <  2:1688061/150‑1 (MQ=255)
                                                                                       tgaccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGa                                                                >  1:4724886/1‑150 (MQ=255)
                                                                                          ccGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACAAGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAg                                                             >  1:5062110/1‑150 (MQ=255)
                                                                                            gAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGAc                                                           >  2:2831628/1‑150 (MQ=255)
                                                                                            gAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGAc                                                           >  2:824335/1‑150 (MQ=255)
                                                                                            gAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGAc                                                           >  2:3361796/1‑150 (MQ=255)
                                                                                              aCGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAg                                                         <  1:3092845/150‑1 (MQ=255)
                                                                                                  gcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCttt                                                     <  2:2839135/150‑1 (MQ=255)
                                                                                                  gcTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCttt                                                     <  1:1888692/150‑1 (MQ=255)
                                                                                                   cTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTg                                                    >  2:3190969/1‑150 (MQ=255)
                                                                                                    tATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGa                                                   <  1:3724761/150‑1 (MQ=255)
                                                                                                     aTGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGAc                                                  >  2:3992153/1‑150 (MQ=255)
                                                                                                      tGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACg                                                 <  1:2109792/150‑1 (MQ=255)
                                                                                                      tGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACg                                                 <  2:4078401/150‑1 (MQ=255)
                                                                                                         gcCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGAtt                                              >  1:259024/1‑150 (MQ=255)
                                                                                                          ccAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTCCAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTc                                             <  2:4142969/150‑1 (MQ=255)
                                                                                                           cAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTcc                                            >  1:858815/1‑150 (MQ=255)
                                                                                                            aGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCa                                           <  1:1490602/150‑1 (MQ=255)
                                                                                                                  cGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTa                                     >  2:5031937/1‑150 (MQ=255)
                                                                                                                  cGCAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTa                                     >  1:4503945/1‑150 (MQ=255)
                                                                                                                   gCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTaa                                    <  1:3390121/150‑1 (MQ=255)
                                                                                                                    cAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAAc                                   >  1:2141977/1‑150 (MQ=255)
                                                                                                                    cAGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAAc                                   >  2:2507922/1‑150 (MQ=255)
                                                                                                                     aGGCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAAcc                                  >  1:1196631/1‑150 (MQ=255)
                                                                                                                       gCACAGCATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTg                                <  1:946249/150‑1 (MQ=255)
                                                                                                                             cATATTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCAGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAg                          >  1:2715350/1‑150 (MQ=255)
                                                                                                                              atatTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGc                         >  2:4788696/1‑150 (MQ=255)
                                                                                                                              atatTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGc                         >  2:2759990/1‑150 (MQ=255)
                                                                                                                               tatTTCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCt                        <  2:4470226/150‑1 (MQ=255)
                                                                                                                                  ttCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAgg                     <  2:3485832/150‑1 (MQ=255)
                                                                                                                                  ttCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCAGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAgg                     <  2:3107067/150‑1 (MQ=255)
                                                                                                                                   tCGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGgc                    <  2:1236106/150‑1 (MQ=255)
                                                                                                                                   tCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACTTATGGTTGCTGCCCAAAGCCACACTTCTAACGAAGAGAAAGTATAAGACTTCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAgtc                    <  1:2994824/150‑3 (MQ=255)
                                                                                                                                   tCGCTGACAAACTTCCTCTACAGCGGCCTGTACACCTGCCTGACCGAACTTCTTGTTGCTGCCCAACGCCACACGTCAAACGAAGATAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGgc                    <  1:225870/150‑1 (MQ=255)
                                                                                                                                    cGCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGgcg                   >  1:3927846/1‑150 (MQ=255)
                                                                                                                                     gCTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGgcgc                  <  1:921945/150‑1 (MQ=255)
                                                                                                                                      cTGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGcgcg                 <  1:1387383/150‑1 (MQ=255)
                                                                                                                                      cTGACCAACTTCCTCTACAGCGGCCTGGACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGcgcg                 <  2:440970/150‑1 (MQ=255)
                                                                                                                                       tGACCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCgg                >  2:1174715/1‑150 (MQ=255)
                                                                                                                                         aCCAACTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGgag              <  1:4296951/150‑1 (MQ=255)
                                                                                                                                              cTTCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACAt         <  1:1110374/150‑1 (MQ=255)
                                                                                                                                               ttCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATc        >  2:513112/1‑150 (MQ=255)
                                                                                                                                                tCCTCTACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCt       >  1:4557294/1‑150 (MQ=255)
                                                                                                                                                  ctctACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTc     >  2:2730652/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTCCTC  >  CP009273/3766851‑3767149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑