Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,783,857 A→T 83.4% I283I (ATT→ATA)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,783,8570AT83.4% 601.2 / 101.5 234I283I (ATT→ATA) tdhL‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (21/18);  new base T (107/88);  total (128/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 235 total aligned reads displayed.

ACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGA  >  CP009273/3783709‑3784002
                                                                                                                                                    |                                                                                                                                                 
  tGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCTCGACCGTAAATACCTTTAATg                                                                                                                                                <  1:4047105/150‑1 (MQ=255)
  tGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATg                                                                                                                                                <  2:399810/150‑1 (MQ=255)
    gCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGaa                                                                                                                                              >  1:4294000/1‑150 (MQ=255)
     cccGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAAc                                                                                                                                             >  1:4941364/1‑150 (MQ=255)
     cccGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAAc                                                                                                                                             >  2:1004575/1‑150 (MQ=255)
     cccGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAAc                                                                                                                                             >  1:3765909/1‑150 (MQ=255)
     cccGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAAc                                                                                                                                             >  1:337247/1‑150 (MQ=255)
      ccGACCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACa                                                                                                                                            <  2:2971097/150‑1 (MQ=255)
      ccGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACa                                                                                                                                            <  2:3575712/150‑1 (MQ=255)
        gAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAg                                                                                                                                          >  1:3268059/1‑150 (MQ=255)
        gAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGAAAATACCGTTTATGAACAAg                                                                                                                                          >  1:3579171/1‑150 (MQ=255)
           cGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCt                                                                                                                                       <  2:3724454/150‑1 (MQ=255)
             cATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCttt                                                                                                                                     <  1:3244154/150‑1 (MQ=255)
              aTAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTa                                                                                                                                    <  2:4760060/150‑1 (MQ=255)
               tAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTaa                                                                                                                                   <  2:4495329/150‑1 (MQ=255)
                   gTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGAt                                                                                                                               >  1:3400985/1‑150 (MQ=255)
                   gTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGAGAATGATCGAGGCCAGACTGAATCAGCGCCGCCATATTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGAt                                                                                                                               <  1:2824784/150‑1 (MQ=255)
                   gTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAAAGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCAAGACCGTAAAAACCTTTAATGAACAAGCCTTTAAatat                                                                                                                               >  2:216303/1‑150 (MQ=255)
                     cAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCa                                                                                                                             >  2:220972/1‑150 (MQ=255)
                     cAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCa                                                                                                                             >  2:4578346/1‑150 (MQ=255)
                     cAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCa                                                                                                                             >  1:1916274/1‑150 (MQ=255)
                      aaaGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGGACCAGGTTTCAAACAGCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCAc                                                                                                                            >  2:1236183/1‑150 (MQ=255)
                       aaGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAGCAAGCCTTTAAAGATCACt                                                                                                                           <  2:773932/150‑1 (MQ=255)
                       aaGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACt                                                                                                                           >  2:4900285/1‑150 (MQ=255)
                       aaGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACt                                                                                                                           <  2:1063540/150‑1 (MQ=255)
                       aaGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACt                                                                                                                           <  2:3359955/150‑1 (MQ=255)
                        aGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACtt                                                                                                                          >  2:3022261/1‑150 (MQ=255)
                          cccTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTg                                                                                                                        <  1:1921415/150‑1 (MQ=255)
                               cttgAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCa                                                                                                                   >  1:3015511/4‑150 (MQ=255)
                               cTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCa                                                                                                                   >  2:3305229/1‑150 (MQ=255)
                                 ggAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGt                                                                                                                 <  1:4893782/150‑1 (MQ=255)
                                   aaaTCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCg                                                                                                               >  2:1917954/1‑150 (MQ=255)
                                     atcatcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGAt                                                                                                             <  1:260087/150‑1 (MQ=255)
                                      tcatcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATa                                                                                                            <  2:3431115/150‑1 (MQ=255)
                                      tcatcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATa                                                                                                            <  2:179791/150‑1 (MQ=255)
                                        atcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGa                                                                                                          >  2:1628283/1‑150 (MQ=255)
                                        atcGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGa                                                                                                          >  1:68873/1‑150 (MQ=255)
                                           gATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACat                                                                                                       >  1:4481004/1‑150 (MQ=255)
                                            aTAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACata                                                                                                      >  1:4428572/1‑150 (MQ=255)
                                              agagAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATc                                                                                                    >  1:45260/1‑150 (MQ=255)
                                               gagaAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCa                                                                                                   <  2:1973558/150‑1 (MQ=255)
                                               gagaAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCa                                                                                                   >  1:1208746/1‑150 (MQ=255)
                                                agaAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAg                                                                                                  >  1:872079/1‑150 (MQ=255)
                                                agaAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAg                                                                                                  >  1:2336539/1‑150 (MQ=255)
                                                agaAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAg                                                                                                  <  1:1589326/150‑1 (MQ=255)
                                                   aaCGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGAcg                                                                                               >  1:2407788/1‑150 (MQ=255)
                                                       aTGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGAcggcgg                                                                                           >  2:293739/1‑150 (MQ=255)
                                                         gggTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGaa                                                                                         <  1:696940/150‑1 (MQ=255)
                                                          ggTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAAt                                                                                        >  2:1156170/1‑150 (MQ=255)
                                                           gTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATa                                                                                       >  1:486485/1‑150 (MQ=255)
                                                              aTGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATAccc                                                                                    >  2:2274932/1‑150 (MQ=255)
                                                                gATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAg                                                                                  <  2:1447730/150‑1 (MQ=255)
                                                                  tCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCa                                                                                >  2:2087320/1‑150 (MQ=255)
                                                                  tCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACATTTTATGAAAAAGCCTTTAAAGATCACTTTTGTACAGTCGATAGACATATAAGACGTCGGAATACCCAgaa                                                                                >  2:4657440/1‑148 (MQ=255)
                                                                    ggagAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATc                                                                              >  1:1756025/4‑150 (MQ=255)
                                                                         aaGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAAt                                                                         <  1:4473602/150‑1 (MQ=255)
                                                                            aTCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACg                                                                      >  1:335277/1‑150 (MQ=255)
                                                                            aTCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACg                                                                      <  2:4988666/150‑1 (MQ=255)
                                                                            aTCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACg                                                                      >  1:4063886/1‑150 (MQ=255)
                                                                               gAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGgcc                                                                   <  1:501471/150‑1 (MQ=255)
                                                                                aGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGgccg                                                                  >  2:629721/1‑150 (MQ=255)
                                                                                  gCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGgccgcc                                                                <  1:2858475/150‑1 (MQ=255)
                                                                                   ccAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGccgccg                                                               <  1:4650036/150‑1 (MQ=255)
                                                                                     aGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTg                                                             <  2:4311631/150‑1 (MQ=255)
                                                                                            aTCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATg                                                      >  2:1297315/1‑150 (MQ=255)
                                                                                              cAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGgt                                                    <  2:2364666/150‑1 (MQ=255)
                                                                                               aGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGgtg                                                   <  2:2439834/150‑1 (MQ=255)
                                                                                                gcgcCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGgtgt                                                  >  2:3342189/1‑150 (MQ=255)
                                                                                                 cgccgcCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTc                                                 >  1:2150824/1‑150 (MQ=255)
                                                                                                  gccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCa                                                >  1:1493959/1‑150 (MQ=255)
                                                                                                        aTCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATg                                          >  2:833911/1‑150 (MQ=255)
                                                                                                           ttGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTa                                       >  2:4195182/1‑150 (MQ=255)
                                                                                                           ttGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTa                                       <  1:220972/150‑1 (MQ=255)
                                                                                                             gTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACg                                     >  1:3140289/1‑150 (MQ=255)
                                                                                                                ccAGGTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGaaa                                  >  1:2762103/1‑150 (MQ=255)
                                                                                                                    gTTTCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCt                              >  2:4425249/1‑150 (MQ=255)
                                                                                                                      ttCAAACATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTgg                            <  2:4919887/150‑1 (MQ=255)
                                                                                                                        cAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcg                          >  2:4750875/1‑150 (MQ=255)
                                                                                                                          aaCATCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcggc                        <  2:2900135/150‑1 (MQ=255)
                                                                                                                             aTCTCACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCa                     >  2:4834575/1‑150 (MQ=255)
                                                                                                                              tctcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAc                    <  2:4134171/150‑1 (MQ=255)
                                                                                                                               ctcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAcc                   >  1:2269357/1‑150 (MQ=255)
                                                                                                                               ctcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAcc                   >  2:4075467/1‑150 (MQ=255)
                                                                                                                               ctcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAcc                   <  2:3611453/150‑1 (MQ=255)
                                                                                                                               ctcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAcc                   >  1:5046606/1‑150 (MQ=255)
                                                                                                                               ctcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCAcc                   >  2:3131882/1‑150 (MQ=255)
                                                                                                                                tcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCg                  >  2:2750075/1‑150 (MQ=255)
                                                                                                                                tcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCg                  <  1:3540464/150‑1 (MQ=255)
                                                                                                                                tcACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCg                  >  2:3105800/1‑150 (MQ=255)
                                                                                                                                 cACGACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCgg                 >  1:1037054/1‑150 (MQ=255)
                                                                                                                                  aCGACCGTAACTACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                <  2:2680985/150‑1 (MQ=255)
                                                                                                                                   cGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGAc               >  2:2227268/1‑150 (MQ=255)
                                                                                                                                    gACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACa              >  1:4641759/1‑150 (MQ=255)
                                                                                                                                    gACCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACa              >  1:1395169/1‑150 (MQ=255)
                                                                                                                                     aCCGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACAt             >  2:3914899/1‑150 (MQ=255)
                                                                                                                                       cGTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACAttt           <  1:2881761/150‑1 (MQ=255)
                                                                                                                                        gTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTc          <  2:872079/150‑1 (MQ=255)
                                                                                                                                        gTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTc          <  2:2336539/150‑1 (MQ=255)
                                                                                                                                        gTAAATACCTTTTATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTc          >  2:3800613/1‑150 (MQ=255)
                                                                                                                                         tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTcc         <  1:3941128/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGA  >  CP009273/3783709‑3784002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑