Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 295,695 T→G 25.4% E317D (GAA→GAC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273295,6950TG25.4% 381.9 / 76.1 223E317D (GAA→GAC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (48/115);  new base G (56/4);  total (104/119)
Fisher's exact test for biased strand distribution p-value = 9.75e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCA  >  CP009273/295547‑295843
                                                                                                                                                    |                                                                                                                                                     
cgtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGtt                                                                                                                                                       <  1:1634394/149‑1 (MQ=255)
    gATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCg                                                                                                                                                   >  2:3870706/1‑149 (MQ=255)
     aTCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCgg                                                                                                                                                  >  2:1113466/1‑149 (MQ=255)
      tCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGc                                                                                                                                                 >  2:1085990/1‑149 (MQ=255)
          gATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgc                                                                                                                                             <  2:2401903/149‑1 (MQ=255)
          gATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgc                                                                                                                                             <  1:3180357/149‑1 (MQ=255)
          gATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTTCggggggc                                                                                                                                             >  1:34209/1‑144 (MQ=255)
           aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgcc                                                                                                                                            <  2:4196048/149‑1 (MQ=255)
             ttCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgccgc                                                                                                                                          <  1:3997180/149‑1 (MQ=255)
             ttCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgccgc                                                                                                                                          <  1:1777706/149‑1 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCgg                                                                                                                                       <  1:4146731/149‑1 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCgg                                                                                                                                       >  2:3812203/1‑149 (MQ=255)
                 aGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTGACGGCGGGCCGCCggg                                                                                                                                      >  1:900527/1‑149 (MQ=255)
                    gcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGGGCCGCCggggcg                                                                                                                                   >  1:1494508/1‑146 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTcc                                                                                                                             <  2:3494396/149‑1 (MQ=255)
                              cGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGAc                                                                                                                         <  1:229466/149‑1 (MQ=255)
                               gggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTCCAGAcc                                                                                                                        >  2:2459296/1‑149 (MQ=255)
                               gggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCGGGCCGGTTTCCCGAcc                                                                                                                        >  2:69046/1‑149 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCa                                                                                                                       <  2:2879303/149‑1 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCCGGCCGGTGTCCAGACCa                                                                                                                       >  1:4362596/1‑149 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGTGCCGCCGGGCAGGTTTCCAGAACa                                                                                                                       >  1:4362739/1‑149 (MQ=255)
                                 gggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCCGGCCGGGTTCCacaacac                                                                                                                      >  1:2614754/1‑143 (MQ=255)
                                 gggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGGAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCCCCCGGCCGGTTTCCCGAcccc                                                                                                                      >  1:4052901/1‑147 (MQ=255)
                                    cTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑tt                                                                                                                  <  2:4355873/149‑1 (MQ=255)
                                      tCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGTGCCCCCGGGCCCGGTTCCCGACCACC‑ATTc                                                                                                                >  2:3155975/1‑149 (MQ=255)
                                         ccGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCAtg                                                                                                             <  1:92831/149‑1 (MQ=255)
                                         ccGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCAtg                                                                                                             <  2:2983921/149‑1 (MQ=255)
                                          cGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCGGTTTGCAGACCACC‑ATTCaatt                                                                                                            >  1:135636/1‑146 (MQ=255)
                                           gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGGGTGGCGCCGGGCAGGGGTCCAGACACAC‑TTTAAtgtg                                                                                                           >  1:1945587/1‑149 (MQ=255)
                                             gcgcACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTgag                                                                                                         <  1:3811202/149‑1 (MQ=255)
                                              cgcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTgaga                                                                                                        <  1:2863284/149‑1 (MQ=255)
                                                 acgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCCGGTTTACACACCAGC‑TTTCATGTGAGGTat                                                                                                     >  2:2259867/1‑148 (MQ=255)
                                                    caTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCgg                                                                                                  <  1:536774/149‑1 (MQ=255)
                                                     aTGGCGTTCCCTTCCGGCAAATCCAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGt                                                                                                 <  2:2597821/149‑1 (MQ=255)
                                                        gCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCGGGCAGGTTTCCAGACCCGC‑TTTCATGTGAGATAGCGGTGAt                                                                                              >  2:3277068/1‑149 (MQ=255)
                                                        gCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGAt                                                                                              >  2:2126751/1‑149 (MQ=255)
                                                              ccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCGGGCCGGTTTGCAGACCAGC‑TGTACTTTGAGATAGGGGTGTttttgac                                                                                        >  1:3844937/1‑146 (MQ=255)
                                                                   cGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGctct                                                                                   <  1:2218937/149‑1 (MQ=255)
                                                                   cGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGctct                                                                                   <  2:1291789/149‑1 (MQ=255)
                                                                   cGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGctct                                                                                   <  2:4287173/149‑1 (MQ=255)
                                                                       aaaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTGCAGACCAGC‑GTTAATGTGAGATAGCGGGGATTTTCCCGCTCTGGTc                                                                               >  2:512318/1‑149 (MQ=255)
                                                                        aaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑GTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCg                                                                              >  2:722943/1‑149 (MQ=255)
                                                                        aaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCCCCGGGCAGGGTTCCAGACCAGC‑GTTCATGTGAGATAGCGGTGATTTTGCCGCGCTGGTCg                                                                              >  1:2458021/1‑149 (MQ=255)
                                                                          tCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGc                                                                            <  1:3895032/149‑1 (MQ=255)
                                                                           cAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGGTCCCCCGGGCCGGTTTCAAGTCCAGC‑TTTGATGTGAGATAGCGGGGTTTTTGCCGCTCTGggcggcc                                                                           >  2:1121812/1‑148 (MQ=255)
                                                                             aGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCCGGCCCGGTTACAGACCACC‑ATTGATTTGTGTTATGGGTGGTTTTACACCACAGGTCGGcccc                                                                         >  1:908863/1‑149 (MQ=255)
                                                                                gTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGAt                                                                      <  2:1225482/149‑1 (MQ=255)
                                                                                gTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCCCCGGGCCGGGTTGCCGACCAGC‑TTTCATGGGAGATAGCGGTGATTTTCCCGCGCTGGTAGGCACCCAt                                                                      >  2:547719/1‑149 (MQ=255)
                                                                                   gCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCCGC‑TTTCATGTGAGATAGCGGTGATTTTACCGCGCTGGTCGGCACCGATACg                                                                   >  2:2540732/1‑149 (MQ=255)
                                                                                      aGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTTCCGCGGGACGCCCGGCCCGTTTACACAACCCC‑GTTCAATTGGGATACACGTGATTTTAACCCTCAGGTAGGCACCCATaaccaa                                                                >  2:821063/1‑144 (MQ=255)
                                                                                       gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCCCCGGGCAGGTTTACAGACCAGC‑GTTCATGTGAGATAGCGGTGCTTTTCCCGCGCTGGTCGGCCCCCATACGCacg                                                               >  2:3960439/1‑147 (MQ=255)
                                                                                          gCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTACAGACCAGC‑TTTAATGTTAGATATCGGTGATTTTACCGCGCTGGTCGGCACCGATACGCACGTGc                                                            >  2:2111675/1‑149 (MQ=255)
                                                                                           cAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCt                                                           <  2:1128135/149‑1 (MQ=255)
                                                                                            aGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGGCGCCCGGCACGTTTACAGACCCGC‑GTTAATGTGATATATCGGTGATTTTACCCCACAGGTCGGCCCCCATAAGCAACTGcag                                                          >  1:700483/1‑147 (MQ=255)
                                                                                               ccGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAg                                                       <  2:2434231/149‑1 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCGGTTTCCAGACCCGC‑TTTCATTTGAGATATCGGTGATTTTAACGCGCTGGGCGGCACCCAAACGCAAGTGCTGAAggg                                                     >  2:3809198/1‑149 (MQ=255)
                                                                                                    aTGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTgg                                                  <  1:2588627/149‑1 (MQ=255)
                                                                                                     tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGGCGCCCGGCCCGGGTACAGACCACC‑ATTCATGTGAGTTATCGGGGGTTTTTCCCCTCGGGTGGGCCCCCGAAAGCAAGTGCGGTACGGTggc                                                 >  2:1138432/1‑149 (MQ=255)
                                                                                                      gACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcg                                                <  2:3407011/149‑1 (MQ=255)
                                                                                                       aCGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCCGCGTGCCCCCCGGCCGGTTTACAGAACAGC‑ATTAATTTGTGATATAGGTTATTTTAACGCTCAGGTAGGCACCGATACGCAAATGCTGAATGGTggcgg                                               >  1:2055671/1‑149 (MQ=255)
                                                                                                        cGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGGGTGCCGCCGGGCAGGTTTCCAGAACAGC‑ATTCATGTGAGATAGCGGTGATTTTACCGCTCGGGTCGGCACCGATAAGCAAGTGCTGAAGGGTggcggg                                              >  2:1723443/1‑149 (MQ=255)
                                                                                                         gATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCCGGCCGGTTTCCAGACCAGC‑TTTCATGTGAGATTGCGGGGATTTTCCCGCGCAGGTCGGCCCCGATACGCAAGTGCTGAAGGGTggcgggg                                             >  2:616814/1‑149 (MQ=255)
                                                                                                         gATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACCGCGTGCCGCCCGGCACGGTTACACACCAACATTACAGGTATAAATGCGG‑GGTTTTACCCCTAAGGTCGGCCACCATACGAAACTGCTGAATGGTggcgggg                                             >  2:579885/1‑149 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGt                                         <  2:3214645/149‑1 (MQ=255)
                                                                                                              gCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTg                                        <  1:106334/149‑1 (MQ=255)
                                                                                                              gCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCGGGCCGGGTTCCAGGCCAGC‑TTTCATGTGAGATAGCGGTGGTTTTCCCCCGCGGGTCGGCCCCCAAACGCCAGTGCGGAAGGGGggcggggcgggg                                        >  2:2718416/1‑147 (MQ=255)
                                                                                                               ccccGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGTGCCGCCCGGCCCGGTTCCCGACCCGC‑ATTCATGTGAGATATCGGGGGTTTTACCGCTCTGGGGGGCACCGCTACGCACGTGCGGAAGGGTggcggggcggggg                                       >  2:3748641/1‑146 (MQ=255)
                                                                                                                   ggCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtgg                                   <  2:1697968/149‑1 (MQ=255)
                                                                                                                   ggCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtgg                                   <  2:1912551/149‑1 (MQ=255)
                                                                                                                    gCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCCGTTTACAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTACCCCTCTGGTCGGCCCCCATAAGCAAGTGCTTAAGGGTGGCGGGGCGGTGCgtggt                                  >  2:1044070/1‑149 (MQ=255)
                                                                                                                      gTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGgtgt                                <  1:1845060/149‑1 (MQ=255)
                                                                                                                       tAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCCCC‑GTTCATGTGAGAAAGCGGGGATTTTCCCCCGCAGGTCGGCACCGAAACGCCAGTGCTGAAGGGTGGCGGGGCGGTGCGGGGGGtt                               >  2:1763852/1‑149 (MQ=255)
                                                                                                                          agTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                            <  2:447221/149‑1 (MQ=255)
                                                                                                                           gTACTTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTg                           <  2:1104326/149‑1 (MQ=255)
                                                                                                                           gTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTg                           <  1:3164309/149‑1 (MQ=255)
                                                                                                                            tAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTgg                          <  1:3435724/149‑1 (MQ=255)
                                                                                                                              aTTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCCCCCGGCAGGTTTCCAGAACACC‑TTTCATGGGAGATAGCGGTGATTTTACCGCGCTGGGGGGCACCCATACGCAACTGCTGTAGGGTGGCGGGGCGGGGCGTGGTGTTATTGGGa                        >  2:4041787/1‑149 (MQ=255)
                                                                                                                               ttCGCTTTGCTGTACCGCCGTGTGCCGGGTGCCGCCCCGCAGGTTTCCAGACCACC‑GATCAATTTGTATAAAGGGTGTTTTTCCGCGCGGGTAGGCACCGATAACCCACTGGAGTAGGGTGGGGGGGCGGTGCGGGGGGTTTTTgggga                       >  2:3722727/1‑147 (MQ=255)
                                                                                                                               ttCGCTTTGCTGTACCGCCGTGTGAGGCGTGCCCCCGGGCCGGTTTACACACCACC‑TTTAATGTGAGATATCGGTGGTTTTACCGCGCTGGTCGGCACCGAAACGCAACTGCTGAAGGGTGGCGGGGCGGGGCGGGGGGTTTTTGGGac                       >  2:2274174/1‑148 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                      <  2:681413/149‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                      <  2:2827160/149‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGGTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                      <  2:3214761/149‑1 (MQ=255)
                                                                                                                                 cGCTTTGCTGGACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                     <  2:2054276/149‑1 (MQ=255)
                                                                                                                                   cTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATaga                   <  1:167657/149‑1 (MQ=255)
                                                                                                                                   cTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATaga                   <  1:3870257/149‑1 (MQ=255)
                                                                                                                                   cTTTGCTGTACCGCCGTTTACGGCGTGCCGCCCGGCAGGTTTACACAACACC‑ATTCAAGTGAGATAGCGGGGCTTTTACCCCACGGGTCGGCCCCCATAAGCAACTGCTGAACGGGGGCGGGGCGGGGCGGGGTGTTATTGGGACTaga                   >  2:3079844/1‑149 (MQ=255)
                                                                                                                                    tttGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCCGGTTTACAGACCAGC‑TTTAATTTGTGATAGAGGTGATTTTACCGCTCTGGTCGGCACCGATACGCAATTGCTGAATGGTGGGGGGGCGGTGGGTGGTGGTTTTGTGtatatat                  >  2:2354498/1‑146 (MQ=255)
                                                                                                                                    tttGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCCCGTTTCCAGACCAGC‑TTTCAAGTGAGATAGCGGTGATTTTACCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATagag                  >  2:2567091/1‑149 (MQ=255)
                                                                                                                                      tGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAggg                <  1:4370848/149‑1 (MQ=255)
                                                                                                                                        cTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGc              <  2:2301817/149‑1 (MQ=255)
                                                                                                                                          gTACCGCCGTTTCCGGCCTGCCGCCGGGCAGGTTTCCAGACCACC‑TTTCAAGTGAGAAAGCGGTGAATTTACCGCTCAGGTCGGCCCCGATAACCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAAAGAGGGGCgg            >  2:2907060/1‑149 (MQ=255)
                                                                                                                                           tACCGCCGTTTGCCGCGTTCCGCCCGGGAGGTTTCAAGACCAGC‑ATTAATTTGAGATAACGGTGATTTTCACCCTCTGGTCGGCACCCCTACGCAAGTGCTGAAGGGTGGCGGGGCCGGGCGTGGTGTTATTGGGAATAGAcgggcggg           >  2:636211/1‑149 (MQ=255)
                                                                                                                                            aCCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCgggg          <  2:1431714/149‑1 (MQ=255)
                                                                                                                                             ccgccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggggg         <  2:3613278/149‑1 (MQ=255)
                                                                                                                                             ccgccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggggg         <  2:1603592/149‑1 (MQ=255)
                                                                                                                                             ccgccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggggg         <  2:3998777/149‑1 (MQ=255)
                                                                                                                                                 cgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCa     <  1:662217/149‑1 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCAt    <  1:2814925/149‑1 (MQ=255)
                                                                                                                                                   tttCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc   >  1:3350487/1‑149 (MQ=255)
                                                                                                                                                    ttCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCa  >  1:4336718/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGC‑TTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCA  >  CP009273/295547‑295843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑