BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,811,275	T→G	6.8%	intergenic (‑56/+143)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,811,275	0	T	G	6.8%	614.1 / 24.1	182	intergenic (‑56/+143)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (68/98); new base G (7/7); total (77/105)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

AAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGC  >  CP009273/2811127‑2811423

aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAt                                                                                                                                                      <  1:4036361/149‑1 (MQ=255)

aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAt                                                                                                                                                      <  1:4036226/149‑1 (MQ=255)

 aGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAtt                                                                                                                                                     <  1:3588326/149‑1 (MQ=255)

  ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTa                                                                                                                                                    >  2:2001760/1‑149 (MQ=255)

     tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAaga                                                                                                                                                 <  1:4227568/149‑1 (MQ=255)

        ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGAT                                                                                                                                              >  1:2634886‑M1/1‑149 (MQ=255)

          agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGG                                                                                                                                            <  1:1680398‑M1/149‑1 (MQ=255)

           gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                           >  2:1743711‑M1/1‑149 (MQ=255)

           gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                           <  2:239516‑M1/149‑1 (MQ=255)

             atatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCTAGTATGAGATGGTGC                                                                                                                                         >  2:4034499‑M1/1‑149 (MQ=255)

              tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCA                                                                                                                                        <  2:2344133‑M1/149‑1 (MQ=255)

               atGGTGCCTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAT                                                                                                                                       <  2:3024731‑M1/149‑1 (MQ=255)

                tGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATC                                                                                                                                      >  2:3638500‑M1/1‑149 (MQ=255)

                tGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATC                                                                                                                                      <  2:13284‑M1/149‑1 (MQ=255)

                 ggTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCC                                                                                                                                     >  1:2375106‑M1/1‑149 (MQ=255)

                  gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCG                                                                                                                                    >  2:1581298‑M1/1‑149 (MQ=255)

                   tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                   <  2:630667‑M1/149‑1 (MQ=255)

                    gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGG                                                                                                                                  <  1:3929478‑M1/149‑1 (MQ=255)

                              ggaTTCGCACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGCGCTACGGATGCATCGGGAAAACTTATTCGACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTc                                                                                                                        <  2:3914208/149‑1 (MQ=255)

                               gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCg                                                                                                                       >  2:2421311/1‑149 (MQ=255)

                                        cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTAGTATACTGCTGATATTGATACCGCTACTAAAGCCATATCAGTTAAGAGAGGGTGCATCCGGGAGGATTAGTCGCCGTGCGGc                                                                                                              >  1:1945357/1‑149 (MQ=14)

                                               cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGATCTTCGGATGCATCTGGATCACTTATTCTACTGCTGATATTGATACAGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                                                       <  1:2043010/149‑1 (MQ=17)

                                               cGCTCGGTTCGTAGCCGAGTACTCTAGCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTTCTGCTGATATTGATACCGCTACTAACGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                                                       <  2:2003327/149‑1 (MQ=255)

                                                gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCtt                                                                                                      <  2:3999477/149‑1 (MQ=255)

                                                 cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACCTTATTCTACTGCTGATATTGAGACCGCTACTAACGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                     <  2:3722373/149‑1 (MQ=255)

                                                 cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                     <  2:3722245/149‑1 (MQ=255)

                                                 cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                     >  2:149226/1‑149 (MQ=255)

                                                 cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                     <  2:3722226/149‑1 (MQ=255)

                                                 atCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCTCTACTAAAGCAATATCTATTATGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                     <  2:2171285/148‑1 (MQ=255)

                                                  tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTTATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCg                                                                                                    <  1:3959184/149‑1 (MQ=255)

                                                       tCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAAAAAGAGAAGGTGAAACCGGGAGGATAACACGCCATCAGGCTCGCGCTTCGCGCCg                                                                                               >  1:3723088/1‑149 (MQ=14)

                                                         gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGATAACTTATACTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                                                             <  1:2293068/149‑1 (MQ=255)

                                                           aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                                                           >  1:515546/1‑149 (MQ=255)

                                                                 gTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTAATTCTACTGCTGATATTGATACAGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTCGGGCCGTTGCTACAGc                                                                                     >  2:4205391/1‑149 (MQ=255)

                                                                   aCTCTATCCAGCTGATCTAAGGATGCATCGGGAAAACTTATTCTACTGCTTATATTGATACCGATACTACAGCCATATCAATTTCGAGATGGTGCCTCCGGGTGGTTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCaa                                                                                   <  1:2308905/149‑1 (MQ=17)

                                                                   aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCaa                                                                                   <  1:3172933/149‑1 (MQ=255)

                                                                   aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCaa                                                                                   <  1:3642856/149‑1 (MQ=255)

                                                                    ctctATCCAGCTGTGCTCCGGCTTCATCTGGACTTCGTATTCGACTGCTGATAATGATACCTCTTCTACAGCCATATCATTTACGAGATGGTGCATCCGGGAGGAGTACTCTCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                  <  1:1485283/149‑1 (MQ=17)

                                                                    ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                  <  1:1961241/149‑1 (MQ=255)

                                                                        aTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCGACTGCTGATATTGAGACCGCTACTAAAGCCATATCACTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                                                              <  2:2621055/149‑1 (MQ=255)

                                                                         tCCAGCTGAGCTACGGATGCATCGGGAACACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                                                             <  1:4009614/149‑1 (MQ=255)

                                                                           cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatca                                                                           <  1:3295956/149‑1 (MQ=255)

                                                                                caGCTACCGATGCATCGGGCAAACATATTCATCGGCTGATATTGACACAGCTACTAAAGCCATATCAATTAAGCGATGGTGCATCCGGGAGTATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCt                                                                      <  2:379562/148‑1 (MQ=17)

                                                                                 aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTCCGGGCCGTTGCTAAAGCAACGTTATCATCCCTa                                                                     >  1:4298630/1‑149 (MQ=255)

                                                                                   cTACGGATGCATCGGGAAAAATTATTCTACTGCTGATATTGTTACCGATACTAAAGACATATCAATTAAGAGATGGTGCGTCCGGGTGGATTACTCGCCGTGCTGATCGCCCTTCGGGCCGTTGCAAAAGAAACGTTATCATGGCTAGt                                                                   >  2:2456861/1‑149 (MQ=18)

                                                                                     aCGGATGCGTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGc                                                                 <  2:2444914/149‑1 (MQ=255)

                                                                                      cGGAGGCATCGGGAAAACTTATTCTACTGATGAGAGTGTTACCGGTTCTACAGCGCAGTCAATTATGTGTCTGTGCATCCGTGTGGTTTAGTCTCCCTGAGGGTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                                                                <  1:4239630/149‑1 (MQ=12)

                                                                                       ggATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                               <  1:2004504/149‑1 (MQ=255)

                                                                                       ggATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTG                                                                                                                                          <  2:2241622‑M1/74‑1 (MQ=255)

                                                                                         aTGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                                                             <  1:2113147/149‑1 (MQ=255)

                                                                                                gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                                                      <  1:2001760/149‑1 (MQ=255)

                                                                                                  ggaaactTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                   <  1:2601114‑M1/68‑1 (MQ=255)

                                                                                                  gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                   >  2:2601114‑M1/1‑70 (MQ=255)

                                                                                                   aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc                                                   <  1:1789638/149‑1 (MQ=255)

                                                                                                     aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc                                                 >  1:4409233/1‑149 (MQ=255)

                                                                                                      aCTTATTCTACTGCTGATATTGATACCGCTACCAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt                                                <  1:4034499/149‑1 (MQ=255)

                                                                                                         tATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc                                             <  1:3683818/149‑1 (MQ=255)

                                                                                                          ctcctcctgcagATTTTTATACAGCTACTACACCCATATCTATTCCGTCATGGGGCGCCCGCGAGGATTAGTCTCAGTGCCGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACct                                            <  1:405183/143‑1 (MQ=9)

                                                                                                             cTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTG                                                                                                                                          >  2:3403787‑M1/1‑52 (MQ=255)

                                                                                                             cTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTG                                                                                                                                          >  2:3402452‑M1/1‑52 (MQ=255)

                                                                                                              tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                        <  2:452464/149‑1 (MQ=255)

                                                                                                              tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                        <  1:1581298/149‑1 (MQ=255)

                                                                                                                ctgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                      <  1:1074271/149‑1 (MQ=255)

                                                                                                                ctgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                      <  1:3527451/149‑1 (MQ=255)

                                                                                                                ctgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                      <  1:3527415/149‑1 (MQ=255)

                                                                                                                ctgctgATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaacc                                      >  1:2788799‑M1/1‑63 (MQ=255)

                                                                                                                 tgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGAt                                     <  1:4305187/149‑1 (MQ=255)

                                                                                                                   ctgATATTGATACCGCTACTACAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAc                                   <  2:2143350/149‑1 (MQ=255)

                                                                                                                     gATATTGATACCGCTACTCAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCt                                 <  1:755587/149‑1 (MQ=255)

                                                                                                                     gATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCt                                 <  1:1182172/149‑1 (MQ=255)

                                                                                                                       tatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTc                               >  1:3028437/1‑149 (MQ=255)

                                                                                                                        atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCa                              >  2:1081309/1‑149 (MQ=255)

                                                                                                                        atTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggc                                                   <  2:3696183‑M1/128‑74 (MQ=255)

                                                                                                                         ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAt                             >  1:528824/1‑149 (MQ=255)

                                                                                                                         ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCAAAAGCAACGTTATCATCCCTATTGCCTGCGGTTAATTCCGCTAACTCTTCGATACATTCAt                             >  1:528797/1‑149 (MQ=255)

                                                                                                                         ttGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggc                                                   >  1:1350441‑M1/1‑54 (MQ=255)

                                                                                                                           gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTg                           >  2:3267368/1‑149 (MQ=255)

                                                                                                                            aTACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGc                          >  2:1971937/1‑149 (MQ=255)

                                                                                                                             tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCt                         <  2:515546/149‑1 (MQ=255)

                                                                                                                             tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCt                         <  2:984274/149‑1 (MQ=255)

                                                                                                                             tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGAGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCt                         >  2:541373/1‑149 (MQ=255)

                                                                                                                             tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCC                                                                                                                                     >  2:3202920‑M1/1‑41 (MQ=255)

                                                                                                                                cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                      >  1:2416012/1‑149 (MQ=255)

                                                                                                                                 gCTACTAAAGCCATATCAATTAAGAGATGGTGCAT                                                                                                                                       >  1:1259573‑M1/1‑35 (MQ=255)

                                                                                                                                 gCTACTAAAGCCATATCAATTAAGAGATGGTGCAT                                                                                                                                       <  2:1259573‑M1/35‑1 (MQ=255)

                                                                                                                                 gCTACTAAAGCCATATCAATTAAGAGATGGTGCAT                                                                                                                                       >  1:3484022‑M1/1‑35 (MQ=255)

                                                                                                                                  ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTACGCTACCTCTTAGATACCTTAATTGCTGAAGa                    >  1:3328699/1‑149 (MQ=255)

                                                                                                                                      taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                >  1:873353/1‑149 (MQ=255)

                                                                                                                                       aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg               <  2:4327066/149‑1 (MQ=255)

                                                                                                                                        aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCGACGTTATCATCCCGAGTGCCTGCGGTTAATTCCGCTCCCTCTTCGATACCTTCATTGCTGAAGATTTCgg              >  1:1180134/1‑149 (MQ=255)

                                                                                                                                          tccATATCTATTAAGAGATGGTGCTTCCGGGAGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTATAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgag            <  1:2719500/148‑1 (MQ=255)

                                                                                                                                          gCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgag            <  1:1462766/149‑1 (MQ=255)

                                                                                                                                             atatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTTGATACCTTCATTGCTGAAGATTTCGGAGAat         <  2:179142/149‑1 (MQ=255)

                                                                                                                                             atatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAat         >  2:1554146/1‑149 (MQ=255)

                                                                                                                                             atatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAat         >  2:519670/1‑149 (MQ=255)

                                                                                                                                                tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg      >  1:3400406/1‑149 (MQ=255)

                                                                                                                                                tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGATATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAGTGCTGAAGATTTCGGAGACTATg      >  2:2789222/1‑149 (MQ=255)

                                                                                                                                                  aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGACTCTGGt    >  2:3732748/1‑149 (MQ=255)

                                                                                                                                                  aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt    <  1:3847987/149‑1 (MQ=255)

                                                                                                                                                  aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt    <  2:2637286/149‑1 (MQ=255)

AAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGC  >  CP009273/2811127‑2811423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`