| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,039,409 | T→G | 16.1% | intergenic (‑149/+118) | ygfF ← / ← gcvP | putative NAD(P)‑dependent oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,039,409 | 0 | T | G | 16.1% | 304.8 / 22.5 | 180 | intergenic (‑149/+118) | ygfF/gcvP | putative NAD(P)‑dependent oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex |
| Reads supporting (aligned to +/- strand): ref base T (48/99); new base G (25/8); total (73/107) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.25e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 184 total aligned reads displayed.
TTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGA > CP009273/3039261‑3039556 | tGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGtt > 2:1745968/1‑149 (MQ=255) tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGttgc > 2:4159052/1‑147 (MQ=255) tttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTaccc > 2:2657684/1‑149 (MQ=255) ttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTccccg > 2:3905800/1‑148 (MQ=255) cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCacac > 1:3807522/1‑149 (MQ=255) cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTgccccccc > 1:2703202/1‑145 (MQ=255) agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAccc < 2:2952506/149‑1 (MQ=255) agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCAccc > 2:1229564/1‑149 (MQ=255) aaaaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCAcccaa > 1:512790/1‑147 (MQ=255) aaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAc < 1:2052286/149‑1 (MQ=255) aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAcccaca > 2:4381201/1‑146 (MQ=255) ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑Tcc < 2:120707/149‑1 (MQ=255) ttCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑Tcc < 2:3165212/149‑1 (MQ=255) cGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑Tcccc < 2:1042242/149‑1 (MQ=255) gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCACACCCAcaccccc‑accccc > 1:953486/1‑148 (MQ=255) ttGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCCC‑TCCCCaa > 2:1411650/1‑149 (MQ=255) aCGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAACCAC‑AACCCaaaag > 2:3083988/1‑147 (MQ=255) gTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCAC‑ACCCCCAAcggg > 1:3981854/1‑149 (MQ=255) gTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTCCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCCCACCCCAAACCCC‑ACCCCCAAcggg > 1:618712/1‑149 (MQ=255) tATCTTTGTTATTGCTAAAAAACTGGCTAAATCTTCATTGTTGGCACGCTTTTCCCCCTCGCCCTTCCTGGGAGTGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGc < 1:2207404/149‑1 (MQ=25) aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCg < 2:1930226/149‑1 (MQ=255) aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAACCAA‑AACCCAAAGGGGCg > 2:2551595/1‑149 (MQ=255) aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGGCCCCACCCCCAAACCCAC‑ACCCCCAACGGGCg > 2:2270048/1‑149 (MQ=255) cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAg < 2:2596019/149‑1 (MQ=255) tttGAGATTGCTAACAAACTGGCTAAATTTTAAATGTTGGCACGGTTTCCCCCTGCCCACTTTCTTGGTGAGGGCCGGGTTGAGGGTATATATTCGCGCCAGTGCCGGCCTGTTCCCCTCTCCCTAACCCTC‑TCCCCAAAGGGGCGAgg < 1:3412249/149‑1 (MQ=21) ttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAggg < 2:3154221/149‑1 (MQ=255) agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGAc < 2:1648192/149‑1 (MQ=255) agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGAc < 2:4119096/149‑1 (MQ=255) gaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACt < 1:3912150/149‑1 (MQ=255) gaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACt < 2:1417168/149‑1 (MQ=255) gaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAACCGC‑TCCCCAAAGGGGCGAggggccg > 2:2062174/1‑146 (MQ=255) aTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCAC‑TCCCCAAAGGGGCGAGGGGACTg > 2:2861659/1‑149 (MQ=255) aTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAACCCAC‑TCCCCAAAGTGGCGGGGGGGCTg > 2:1968231/1‑149 (MQ=255) ttGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGt < 1:3759285/149‑1 (MQ=255) ttGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCGC‑ACCCCCCAGGGGCGGGGGGGCTGt > 2:3885781/1‑149 (MQ=255) ttGCTAACAAACGGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGt < 1:3757703/149‑1 (MQ=255) tGCTAACAAACTGGCTCAATCTTCATTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGTGGGCCGGGGTGTGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTc < 1:3003166/149‑1 (MQ=255) gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCGCAACCAAAACCAC‑AACCCAAACGGGGGTGGGGGCTGtat > 1:359105/1‑147 (MQ=255) cTAACACACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTg < 2:2407203/149‑1 (MQ=255) aaCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAAACCAC‑ACCCCAAACGGGCGCGGGGCCGGTCagag > 2:3418913/1‑149 (MQ=255) aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGc < 2:1637743/149‑1 (MQ=255) aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCGCCCCCAAACCCCC‑ACACCACAAGGGGGGGGGGGCTGtgggtgg > 1:1953869/1‑143 (MQ=255) caaCTGGCTCAATCTTCAAGGTTGGCACGCTGTTCCCCCTCGCCCTTTCCGGGAGAGGGCCGGGGGGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCTC‑TCCCCACAGGGGCGAGGGGACTGTCTGAGCA‑c < 1:4087837/148‑1 (MQ=255) aaaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑c < 1:2413085/149‑1 (MQ=255) cTGGCTCAATCTTCATTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑Cttt < 1:3875768/149‑1 (MQ=255) cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑Cttt < 2:1260543/149‑1 (MQ=255) cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTGGCCCGCACCCAAAACCACAACCCCAAAGGGG‑GGGGGGACAGTGTGAGCA‑Catt > 1:4365204/1‑149 (MQ=255) gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGAAACCTC‑TCCCCAAAGGGGGGAGGGGACTGTCTGAGCA‑CGTTTGt > 2:317996/1‑149 (MQ=255) gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCAC‑TACCCCAAGGGGCGAGGGGCCGGTCGTCGCC‑CTTTTGt > 2:2520348/1‑149 (MQ=255) tCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGGGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTg < 2:1103124/149‑1 (MQ=255) cTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCAAAACCAC‑TCCCCAAAGGGGCGCGGGGCCTGTCTGTGGA‑CGTTTGTAAttttt > 2:916031/1‑147 (MQ=255) ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAAAACAA‑ACCCCCAAAGGGCGAGGGGACAGTCTGAGCA‑AATTTGTACTTTGTc > 1:4052099/1‑149 (MQ=255) aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCACCCAAACCCCC‑TCCCCAAAGGGGCGAGGGGACGGTCTGAGCA‑CATTTGTACTTTGTCATc > 2:4047222/1‑149 (MQ=25) aTGTTGGCACGCTTTTCCCCCCCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCt < 1:1494763/149‑1 (MQ=255) ttGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCAC‑TCACCAAAGGGGCGGGGGGACTGTCTGAGCA‑CGTTTGTTCGTGTTCCTCTGAc > 2:3456988/1‑149 (MQ=255) ggCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTa < 2:3564268/149‑1 (MQ=255) cGCTTTCCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTaaaaa < 1:2396464/149‑1 (MQ=255) ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGgc < 2:3270954/149‑1 (MQ=255) ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGgc < 2:2787704/149‑1 (MQ=255) ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAAAACAC‑TCCCCAAACGGGCGAGGGGACTCTCAGAGCA‑CGATTGTACATTTTAAACTGACTaaaaatga > 2:2791952/1‑146 (MQ=255) ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCCAAACCAC‑ACCCCAAACGGGCGAGGGGCCTGTCTGAGCA‑CTTTTGTACTTTGTCATAGGACTAAAAAGgc > 2:4263831/1‑149 (MQ=255) ttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCAC‑TCCCCCAAGGGGCGGGGGGACAGGCTGAGCA‑CATTTGGTCTTTGGCATCTGACTAAAAAGgcgc > 2:3295864/1‑149 (MQ=255) ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAg < 2:1488283/149‑1 (MQ=255) ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAg < 2:1486493/149‑1 (MQ=255) cTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgc < 2:3416968/149‑1 (MQ=255) tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCACACCCAAAACCAC‑ACCCCCAACCGGCGCGGGGCGAGTCTGAGGC‑CGTTTTTTATTTTTGATATTACAAAAAAAGCGCCCAAccg > 1:4303599/1‑149 (MQ=255) gCCCTGTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGcc < 2:2778737/149‑1 (MQ=255) tttCTGGGAGAGGGCGGGGGTGAGGGTAAATTTTCGCGCCAGTGCCGGCGTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa < 1:2299732/149‑1 (MQ=255) tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa < 2:4159919/149‑1 (MQ=255) tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCAC‑ACCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CATTTGTACTTTGTAATCTGACTAAAAATGCGCCGAAGCGCCGTTa > 2:691612/1‑149 (MQ=255) cAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaa < 2:517098/149‑1 (MQ=255) gggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa < 2:2377586/149‑1 (MQ=255) agagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCAC‑ACCCCAAAAGGGCGCGGGGTCAGTCTGTGCA‑CGTTTTTTATTTGTCATCTGACTAAAAATGCGCCGAATCGCCGTTAGaaaaaat > 2:3639191/1‑146 (MQ=255) gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGt < 1:1299991/149‑1 (MQ=255) agGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc < 2:569521/149‑1 (MQ=255) agGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc < 2:570501/149‑1 (MQ=255) agGGCCGGGGTGAGGGTAAAGATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc < 2:973332/149‑1 (MQ=255) gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGaa < 2:1836277/149‑1 (MQ=255) ggggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCAAAACCACGACCCCAAAGGGG‑GTGGGGGCGGTGGGGGCC‑CGTTTGTTCTTTGTGATCTGTCTAAAAAATGGCCGCAGCGCCAGTAGAAAATAGTCgcctca > 2:4096455/1‑144 (MQ=255) gggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCAC‑TCCCCAAAGGGGCGAGGGGACAGTCTGTGCC‑CTTTTGTACGTTGTCATCTGACTAAAAAGGCGCCGACGCGCCGTTAGAAAATAGTCGAATCAg > 2:2144591/1‑149 (MQ=37) gTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTg < 2:3224353/149‑1 (MQ=255) gTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACCCCCAAACCCAC‑ACCCCCAACGGGCGCGGGGCCAGTCTGAGCC‑CATTTGTACGTTGTCAACAGACAAAAAACGCGCCGAACCGCCCGTAGAAAAAAATCGAAACacag > 2:63105/1‑146 (MQ=255) tGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGa < 1:2050675/149‑1 (MQ=255) gggTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCACACCCAAAACCAC‑AACCCAAATGGGCGAGGGGGCTGTGTGATCA‑CGTTTGTACTTTGTGATATTTCAAAAAAATCGCCCAAGCGCCTGTAGAAAAAATTGGAATAAGTTAAtt > 2:3195334/1‑149 (MQ=255) gggTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAAtt < 2:3373334/149‑1 (MQ=255) gggTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAACCAAACCCAC‑ACCCCACAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTTCTTTGTCATATTACAAAAAATGCGCCGAAGCGCCGTTAGAAAAAAGTCGAATCAGTGAAtt > 2:4013058/1‑149 (MQ=255) gTAAATAGTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTAc < 1:1191884/149‑1 (MQ=255) aaaTATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg < 1:3964014/149‑1 (MQ=255) aaaTATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg < 1:1745968/149‑1 (MQ=255) aaaTATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg < 1:1229564/149‑1 (MQ=255) tcttCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTa < 1:2248738/147‑1 (MQ=255) atTCGCGCCAGTGCCGGCCTGGTACCCCCCCCCCAACCCCC‑ACCCCAAACGGGCGCGGGGGCAGTCGGAGCACCGTTTGT‑CAGTGTAAACAGACAAAAAAAGCGCCCAACCGCCCGTAGTAAAAAATCGAAACAGGGAAATaaagggat > 2:1348427/1‑142 (MQ=255) ttCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTAtt < 1:179255/149‑1 (MQ=255) gcgcCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGc < 2:4417581/149‑1 (MQ=255) cAGTGCCGGCCTGTTCCCCACACCCAAAACCAC‑TACCCAAAGGGGCGAGGGGCCGGTCTGAGCA‑CATTTGTACATTTTCAAATGAATAAAAAGGCGCCGCAGCGCCATTAGAAAAAAATCGAAAAAGTGAATTACTTGTATTATCTAAt > 2:1929466/1‑149 (MQ=255) cAGTGCCGGCCTGGTCCCCGCACCCTCACCCGC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTaag < 1:520073/149‑2 (MQ=255) gCCTGGTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGc < 1:2824295/149‑1 (MQ=255) ccTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAATGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCa > 2:933143/1‑149 (MQ=255) cTGTTCCCCTCACCCTAACCCTC‑TCCCCACAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg < 1:2579965/149‑1 (MQ=255) gTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTCTTCGCTAATCGGTACGCCGGa > 2:1540740/1‑149 (MQ=255) | TTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACTGTCTGAGCA‑CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGA > CP009273/3039261‑3039556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |