Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,339 A→C 7.6% Q12P (CAA→CCA)  xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3390AC 7.6% 540.4 / 3.8 218Q12P (CAA→CCA) xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (110/84);  new base C (16/8);  total (126/92)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTG  >  CP009273/3822192‑3822487
                                                                                                                                                    |                                                                                                                                                     
  taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCCAcc                                                                                                                                                    >  2:3117958/1‑149 (MQ=255)
   aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTGTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCg                                                                                                                                                   <  2:3790769/149‑1 (MQ=255)
     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGCGTCA‑GAAAATGCGCCACCGGt                                                                                                                                                 >  2:3053043/1‑149 (MQ=255)
     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGt                                                                                                                                                 >  1:4044120/1‑149 (MQ=255)
      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGtt                                                                                                                                                >  2:1933834/1‑149 (MQ=255)
       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTg                                                                                                                                               <  1:1251828/149‑1 (MQ=255)
       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGTTg                                                                                                                                               >  1:1762950/1‑149 (MQ=255)
        aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTgc                                                                                                                                              <  2:3513016/149‑1 (MQ=255)
        aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGTTgc                                                                                                                                              >  1:1656903/1‑149 (MQ=255)
         gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCCACCGGTTgcg                                                                                                                                             >  2:1152311/1‑149 (MQ=255)
         gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAAACGGTTtcg                                                                                                                                             >  1:1930451/1‑149 (MQ=255)
         gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAAACCGTTgcg                                                                                                                                             >  1:482076/1‑149 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGGTgcgc                                                                                                                                            >  1:4338376/1‑149 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTCTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTgcgc                                                                                                                                            >  1:3983943/1‑149 (MQ=255)
              tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                        >  2:2764928/1‑149 (MQ=255)
                tATCTCATTTAAATATTTAGATGAGGATGTTGAAAACGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTGTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCTAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTc                                                                                                                                      <  2:3484361/149‑1 (MQ=255)
                 aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                     >  2:1641625/1‑149 (MQ=255)
                    tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                  >  2:1207018/1‑149 (MQ=255)
                    tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGACAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                  <  2:1342970/149‑1 (MQ=255)
                    taaTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGACAGATAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                  <  2:1067390/147‑1 (MQ=255)
                     cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                                 >  2:3239397/1‑149 (MQ=255)
                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                               >  2:4010622/1‑149 (MQ=255)
                        ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                                                                                                              >  2:1829297/1‑149 (MQ=255)
                         tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTTCGCAGACACAAAACAgag                                                                                                                             >  1:2876573/1‑147 (MQ=255)
                         tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCg                                                                                                                             >  2:1907230/1‑149 (MQ=255)
                         tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCg                                                                                                                             >  1:1448280/1‑149 (MQ=255)
                            atatTTAGATTAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGAGGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          <  2:105720/149‑1 (MQ=255)
                            atatTTAGATGAGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTTTTTTCGATGTATGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          <  2:2064560/149‑1 (MQ=255)
                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGA‑t                                                                                                                     <  2:1420344/149‑1 (MQ=255)
                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGA‑t                                                                                                                     >  2:2438097/1‑149 (MQ=255)
                                 tAGATGAGGATGTGGAAAACGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGGTTTTGTTTTCTTTGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGA‑tt                                                                                                                    <  2:1874475/149‑1 (MQ=255)
                                  aGATGATGATGTGGCAAACGTTTGCTTTTCTCTTACGGTCAGCTAAAATGCCCGATTTGCTACCACGGTATTGTTTTCTATGTCTGTTTCCCCCCTCGAGACA‑CAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑ttt                                                                                                                   <  2:62055/149‑1 (MQ=255)
                                  aGATGAGTTTTTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGATGTGCTACCACGGTATTGTTTTCGATGACTTTTTCCACCCTCGAGTAA‑CAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGA‑ttt                                                                                                                   <  2:62047/149‑1 (MQ=255)
                                   gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTa                                                                                                                  >  2:3661408/1‑149 (MQ=255)
                                   gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAACGAACAGA‑TTTa                                                                                                                  >  1:3611126/1‑149 (MQ=255)
                                     tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTAcc                                                                                                                >  2:4287703/1‑149 (MQ=255)
                                      gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACACCGAACAGA‑TTTACCg                                                                                                               >  2:3981121/1‑149 (MQ=255)
                                       aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCACACGCAAAAAACCGAACAGA‑TTTaacct                                                                                                              >  1:3913582/1‑145 (MQ=255)
                                       aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGGTGCGCAGACACAAAAAAGCGAACTGA‑TTTACCGt                                                                                                              >  1:1366867/1‑149 (MQ=255)
                                              gtcAAACGTTTGATTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGACTGTTACCACCCTCGAGTCA‑GAAAATGCGCAACGGTTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACGGTCTTGAAg                                                                                                       <  2:1530956/147‑1 (MQ=255)
                                              ggCAACCGTTTGCTTGGCTCTTTCGGTCCGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACGGTCTTGAAg                                                                                                       <  2:3376393/149‑1 (MQ=255)
                                                cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGAt                                                                                                     >  2:3588090/1‑149 (MQ=255)
                                                aaaaGGTTTGCTTTTCTCTTTCGGTCAGACAAAATGCCCGATTTGCTACCTCGGTTTTTTTTTCGTTGTGTGTTTCCCCCCTCTAGTCA‑TAAAATGCCCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGAt                                                                                                     <  1:3295600/148‑1 (MQ=255)
                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCg                                                                                                   >  2:2111484/1‑149 (MQ=255)
                                                    cttttGCTTTTCTCTTTCGGTCAGATAAAGTGCCCGCTTTGCTACCCGGGGTTTGTTTTCTATGTGTGTTTCCACCCTGGTGTCA‑TAAATGGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTc                                                                                                 <  1:1981940/147‑1 (MQ=255)
                                                    cGTGTGCTTTTCTCTTTCGGTCCGCTAAAATGCCCGGTTTGCTACCCCGGGTTGGTTTTCGTTGTCTGTTTCCCCCCGCGAGTCA‑GAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTc                                                                                                 <  2:2221992/149‑1 (MQ=255)
                                                      tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGGTGCGCCGACACAAAAAACACAACAGA‑TTTAACGTCTTGTATATCGTccg                                                                                               >  1:2660334/1‑149 (MQ=255)
                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCACCCCGGTGCGCAGACACAAAAAAACGAACAGA‑TTTAACCTCTTTAAGATCGTccgc                                                                                              >  1:2990381/1‑149 (MQ=255)
                                                        tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTccgcc                                                                                             >  2:1675725/1‑149 (MQ=255)
                                                        tGCTTTTCTCTTTCGGTCAGCAAAATTGCCCGGTTTGCTACCAGGGTTTTTTTTTCGTTTTTTGTTCCCACCCTCGAGCCC‑TAAATTGCCCAACCGGTGGCCCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTccgcc                                                                                             <  1:2521409/149‑1 (MQ=255)
                                                          cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAACTGAGTTTACC‑TCTTTAATATAGTccgcccc                                                                                           >  1:642559/1‑147 (MQ=255)
                                                            tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACACAAAACAACGAACAGA‑ATTACCCTCTTGAAGATCGTcccccccgt                                                                                         >  1:4382363/1‑145 (MQ=255)
                                                              tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                       <  2:859193/149‑1 (MQ=255)
                                                                  tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGGGCCACCGGTTGCGCAGACTCAAAAAAGCGAAAAGG‑TTTAGAGTCTTGTAGATCTTCCGCCGCTTCCTCaa                                                                                   >  1:3079724/1‑149 (MQ=255)
                                                                            atAAATTGCCTGCTTTGCTACTCGGGTTTTGTTTTCTTTGTTTGTTTCCACCCTCTAGTCATAAAAAGGCCCCCCCGGTGGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                          <  1:229678/148‑1 (MQ=255)
                                                                             tAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                        <  2:856181/149‑1 (MQ=255)
                                                                             tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCTGTTTgc                                                                        >  2:2707001/1‑149 (MQ=255)
                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                    <  2:3815261/149‑1 (MQ=255)
                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAACAGA‑TTTAACGTCTTTAATATCGTCAGCCGCTTCCACAAAACCAGTTTtgcgcc                                                                    >  1:198411/1‑149 (MQ=255)
                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCCGGTGCGCAGACGCAAAACAGCGAACAGA‑TTTAACGTCTTGAATATCGTCCGCCCCTTCCTCAAACCCGGGTTgtcgcc                                                                    >  1:198369/1‑149 (MQ=255)
                                                                                  tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                   <  2:885035/149‑1 (MQ=255)
                                                                                  ggCCCGCTTTGCTACCAGGTGATTTTTTTCGTTGTCTGTGTCCCCCCTCGAGACA‑TAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                   <  1:3629640/148‑1 (MQ=255)
                                                                                   gCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTTCCGCCTg                                                                  >  2:3145218/1‑149 (MQ=255)
                                                                                      cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCCACCCGTTGCGCACACGCAAAACACCGCACTGA‑TTTACCCTCTTTAAGATCGTCCGCCCCGTCCACAAAACCAGTTTGCCGCCGGTCa                                                               >  1:3558111/1‑149 (MQ=255)
                                                                                      cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAAATGA‑TTTACCGTCTTGAAGATCGTACGCCGCTTCCTCAAAACCGGTTTGGCGCCTGTCa                                                               >  2:2431013/1‑149 (MQ=255)
                                                                                        cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                             >  2:3467862/1‑149 (MQ=255)
                                                                                         tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑TAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                            <  2:2291557/149‑1 (MQ=255)
                                                                                                   cGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAAACGGGTGCGCAGACTCAAAAAACCGAACAGA‑TTTAACCTCTTGAAGATAGGCCCCCGCGTCCACAAAACCGGTTTGACGCCAGTCCGCATCTGCTGGCg                                                  >  1:1169182/1‑149 (MQ=255)
                                                                                                    gggATTTTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                 <  2:3752679/149‑1 (MQ=255)
                                                                                                        gtGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                             <  2:4149330/148‑1 (MQ=255)
                                                                                                         ttttttCGTTGTCTGTTTCCACCCTCGTGTAA‑TAAATTGCGAAACCTGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                            <  2:401354/147‑1 (MQ=255)
                                                                                                         tGTTTTCGTTGTCTGTTCCCCCCCTCGAGTCA‑TAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                            <  1:1829297/149‑1 (MQ=255)
                                                                                                                gttgtgtgTTCCCACCCTCGCGCCA‑CAAACGGCCCAACGGGTGGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                     <  1:1331785/147‑1 (MQ=255)
                                                                                                                gATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                     >  2:713079/1‑149 (MQ=255)
                                                                                                                gATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCGGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                     >  2:4056031/1‑149 (MQ=255)
                                                                                                                  tGTCTTTTCCCACCCGCGAGTCA‑GAAAATGCGCACCGGGGGGCCCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                   <  1:2733686/149‑1 (MQ=255)
                                                                                                                   gTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                  >  2:2489220/1‑149 (MQ=255)
                                                                                                                    tCTGTTACCCCCCTCGAGTAA‑GAAAAGGCGCAACCGGTGGCCCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                 <  1:2004465/149‑1 (MQ=255)
                                                                                                                    tCTGTCACCACCCTCGAGTCA‑GAAACTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                 <  2:90940/149‑1 (MQ=255)
                                                                                                                     cTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                <  2:4338376/149‑1 (MQ=255)
                                                                                                                         taccccccGCGAGTCA‑GAAACGGCGCACCGGGGCGCCCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                            <  1:4204797/144‑1 (MQ=255)
                                                                                                                           ccACCCTCGAGCCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                          <  2:2809278/149‑1 (MQ=255)
                                                                                                                                cTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGA‑TTTAACGTCTTTAAGATCGTACGCCGCTTCCTCAAAACCAGTTTTCCGCCAGTAAGCAACTGCTGGCGATGTTAGTTGAGGTGTTAACGCCAgcgca                     >  1:2194420/1‑148 (MQ=255)
                                                                                                                                cTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAAAACCGAACTGA‑TTTAACGTAATTAATATAGTACGCCCCTTCCACAAAACCTTTTTTCCGCCTGTCATCAACAGCTGGCGGTGGTAGGTTAGGTTATAACGCCAGCGCt                     >  2:763049/1‑149 (MQ=255)
                                                                                                                                       tctgAAATTGCGCAAGGGTTGGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt               <  1:1575202/146‑1 (MQ=255)
                                                                                                                                       cA‑GAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc              <  2:3794334/149‑1 (MQ=255)
                                                                                                                                       cA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc              >  2:3176102/1‑149 (MQ=255)
                                                                                                                                        a‑GAAAATGCGCCACGGGGCCCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt             <  1:2431013/149‑1 (MQ=255)
                                                                                                                                          gAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTTA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAACCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg            >  2:4411806/1‑149 (MQ=255)
                                                                                                                                            aattGCGCAACGGGTGGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc          <  1:2938945/146‑1 (MQ=255)
                                                                                                                                            aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc          >  2:4257721/1‑149 (MQ=255)
                                                                                                                                            aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc          >  2:548299/1‑149 (MQ=255)
                                                                                                                                            aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc          >  2:2748457/1‑149 (MQ=255)
                                                                                                                                            aaaTGCCCACCGGGTGCCCCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc          <  1:2998700/149‑1 (MQ=255)
                                                                                                                                             aaTGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa         <  2:2593269/149‑1 (MQ=255)
                                                                                                                                              ttGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg        <  2:673423/148‑1 (MQ=255)
                                                                                                                                              ctGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg        <  2:1762950/148‑1 (MQ=255)
                                                                                                                                                  gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc    >  1:840260/1‑149 (MQ=255)
                                                                                                                                                  gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc    >  1:840245/1‑149 (MQ=255)
                                                                                                                                                    aaCCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg  >  2:4196362/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCA‑GAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGA‑TTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTG  >  CP009273/3822192‑3822487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑