Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 505,336 C→T 12.1% E500E (GAG→GAA)  copA ← copper transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273505,3360CT12.1% 858.9 / 78.4 274E500E (GAG→GAA) copAcopper transporter
Reads supporting (aligned to +/- strand):  ref base C (121/118);  new base T (13/20);  total (134/140)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

GCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGCCG  >  CP009273/505190‑505481
                                                                                                                                                  |                                                                                                                                                 
gCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTc                                                                                                                                                 >  2:4683957/1‑149 (MQ=255)
    gcgcTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGAGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgcc                                                                                                                                             >  2:4845338/1‑149 (MQ=255)
       cTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAgccgcc                                                                                                                                          <  2:5110414/149‑1 (MQ=255)
        ttCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCCAATTCAGccgccc                                                                                                                                         >  1:4356958/1‑149 (MQ=255)
        ttCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCCAACTCAGccgccc                                                                                                                                         >  2:3935403/1‑149 (MQ=255)
        ttCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgccc                                                                                                                                         <  1:3507782/149‑1 (MQ=255)
        ttCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgccc                                                                                                                                         <  1:3508260/149‑1 (MQ=255)
          catcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGccgcccgc                                                                                                                                       >  1:3069748/1‑149 (MQ=255)
           atcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTAAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCcgcccgcc                                                                                                                                      >  1:3995389/1‑149 (MQ=255)
            tcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCgcccgccc                                                                                                                                     <  1:4388114/149‑1 (MQ=255)
            tcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCgcccgccc                                                                                                                                     <  1:2696813/149‑1 (MQ=255)
            tcaACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCgcccgccc                                                                                                                                     >  2:4920893/1‑149 (MQ=255)
                  tCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGcc                                                                                                                               >  2:3588625/1‑149 (MQ=255)
                      cAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGaa                                                                                                                           >  2:1396376/1‑149 (MQ=255)
                        aaTGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaat                                                                                                                         >  2:1725754/1‑149 (MQ=255)
                         aTGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAaata                                                                                                                        >  2:3983977/1‑149 (MQ=255)
                               tCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGAc                                                                                                                  >  1:1668816/1‑149 (MQ=255)
                               tCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGAc                                                                                                                  <  1:3243726/149‑1 (MQ=255)
                                cACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACa                                                                                                                 >  2:2493288/1‑149 (MQ=255)
                                cACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACa                                                                                                                 >  2:2201989/1‑149 (MQ=255)
                                cACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACa                                                                                                                 >  2:1265163/1‑149 (MQ=255)
                                cACTGAGACAACCTGCGGCTTCCCTTCATTCAGCGTCCCGTTTTTATCTAAAACTAAAGTGTCGAGTGTACTTGCGCGTTGAAGCGCGTCAGCGTCACGCACCAGCACGCCAAAATCAGCCGCCCGCCCGACGCCGGAAATAATCGACa                                                                                                                 <  1:2143343/149‑1 (MQ=255)
                                 aCTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACAt                                                                                                                >  1:1085866/1‑149 (MQ=255)
                                 aCTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACAt                                                                                                                >  1:1085441/1‑149 (MQ=255)
                                 aCTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACAt                                                                                                                <  2:831649/149‑1 (MQ=255)
                                 aCTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACAt                                                                                                                <  2:66348/149‑1 (MQ=255)
                                 aCTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACAt                                                                                                                <  1:3622476/149‑1 (MQ=255)
                                 aCTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACAt                                                                                                                <  1:2349957/149‑1 (MQ=255)
                                      gACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCg                                                                                                           >  2:4434791/1‑149 (MQ=255)
                                        cAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTc                                                                                                         <  1:2637570/149‑1 (MQ=255)
                                         aaCCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCg                                                                                                        >  2:1992091/1‑149 (MQ=255)
                                            cTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCa                                                                                                     >  1:242259/1‑149 (MQ=255)
                                             tGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAg                                                                                                    <  1:113150/149‑1 (MQ=255)
                                              gCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGc                                                                                                   >  1:96893/1‑149 (MQ=255)
                                                 gCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGcccc                                                                                                >  2:10982/1‑149 (MQ=255)
                                                   ttCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAg                                                                                              <  1:5199338/149‑1 (MQ=255)
                                                       cTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGca                                                                                          >  1:282342/1‑149 (MQ=255)
                                                         tcagtcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGcaca                                                                                        >  2:4504393/1‑149 (MQ=255)
                                                         tcagtcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCAGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGcaca                                                                                        <  2:1544650/149‑1 (MQ=255)
                                                            gtcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACgg                                                                                     >  2:3518228/1‑149 (MQ=255)
                                                            gtcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACgg                                                                                     >  1:2036819/1‑149 (MQ=255)
                                                             tcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGa                                                                                    <  2:2622372/149‑1 (MQ=255)
                                                             tcagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGa                                                                                    <  1:1931310/149‑1 (MQ=255)
                                                              cagCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGAc                                                                                   <  1:1396454/149‑1 (MQ=255)
                                                               agCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACa                                                                                  >  1:4958552/1‑149 (MQ=255)
                                                                gCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCCGCGCACACGGACAg                                                                                 >  1:633940/1‑149 (MQ=255)
                                                                gCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAg                                                                                 >  2:3347534/1‑149 (MQ=255)
                                                                   tCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCa                                                                              <  1:4739727/149‑1 (MQ=255)
                                                                   tCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCa                                                                              >  1:388600/1‑149 (MQ=255)
                                                                      cGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaata                                                                           <  2:2921723/149‑1 (MQ=255)
                                                                       ggTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaataa                                                                          >  2:3083721/1‑149 (MQ=255)
                                                                       ggTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaataa                                                                          >  1:3334456/1‑149 (MQ=255)
                                                                        gTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCaataat                                                                         >  2:1588128/1‑149 (MQ=255)
                                                                             aTCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTa                                                                    <  2:4973535/149‑1 (MQ=255)
                                                                             aTCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTa                                                                    <  2:257740/149‑1 (MQ=255)
                                                                              tCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTAc                                                                   <  2:1479476/149‑1 (MQ=255)
                                                                              tCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTAc                                                                   >  2:3538006/1‑149 (MQ=255)
                                                                              tCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTAc                                                                   >  1:4483084/1‑149 (MQ=255)
                                                                                gAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCg                                                                 >  1:3732260/1‑149 (MQ=255)
                                                                                 aaCACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgt                                                                >  1:508176/1‑149 (MQ=255)
                                                                                  acacTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCgtg                                                               <  1:1784527/149‑1 (MQ=255)
                                                                                         aGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCaa                                                        <  1:5174289/149‑1 (MQ=255)
                                                                                         aGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCaa                                                        <  1:1900102/149‑1 (MQ=255)
                                                                                            gtCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCa                                                     <  2:4612810/149‑1 (MQ=255)
                                                                                             tCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCAc                                                    <  2:1085441/149‑1 (MQ=255)
                                                                                                aGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCCGCCCACACGGACAGGCAATAAACCTTACCGTGGTGGCAAACACCAg                                                 >  2:3471018/1‑149 (MQ=255)
                                                                                                 gtgtACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAgg                                                >  1:247206/1‑149 (MQ=255)
                                                                                                 gtgtACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAgg                                                <  2:285028/149‑1 (MQ=255)
                                                                                                   gtACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGt                                              <  1:3547480/149‑1 (MQ=255)
                                                                                                    tACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGta                                             >  2:3048314/1‑149 (MQ=255)
                                                                                                      cTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCACCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGtata                                           <  2:140117/149‑1 (MQ=255)
                                                                                                       tGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAg                                          <  1:4823384/149‑1 (MQ=255)
                                                                                                        ggCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCGAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGa                                         <  2:5186164/149‑1 (MQ=255)
                                                                                                        ggCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGa                                         <  1:2940316/149‑1 (MQ=255)
                                                                                                        ggCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGa                                         >  2:20912/1‑149 (MQ=255)
                                                                                                           gcgTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACaa                                      <  2:4420608/149‑1 (MQ=255)
                                                                                                           gcgTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACaa                                      <  2:4085670/149‑1 (MQ=255)
                                                                                                            cgTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAAt                                     <  1:5139185/149‑1 (MQ=255)
                                                                                                             gTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATc                                    <  1:2162475/149‑1 (MQ=255)
                                                                                                              ttGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCt                                   >  2:3686006/1‑149 (MQ=255)
                                                                                                              ttGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCt                                   <  2:2081714/149‑1 (MQ=255)
                                                                                                                 cAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCg                                <  2:5045487/149‑1 (MQ=255)
                                                                                                                  aGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCAACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCgg                               <  1:4229406/149‑1 (MQ=255)
                                                                                                                   gcgcGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGt                              >  2:1880512/1‑149 (MQ=255)
                                                                                                                      cgTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGcc                           <  1:3432504/149‑1 (MQ=255)
                                                                                                                         cAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGa                        <  1:80754/149‑1 (MQ=255)
                                                                                                                              tCCCGCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCaaa                   <  2:2217117/149‑1 (MQ=255)
                                                                                                                                ccGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGa                 <  2:4181882/149‑1 (MQ=255)
                                                                                                                                ccGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGa                 >  2:1191936/1‑149 (MQ=255)
                                                                                                                                  gCACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGaaa               <  2:4456734/149‑1 (MQ=255)
                                                                                                                                  gCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGaaa               <  2:1324742/149‑1 (MQ=255)
                                                                                                                                   cACCAGCACGCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAAt              <  1:1188584/149‑1 (MQ=255)
                                                                                                                                   cACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAAt              <  1:1979805/149‑1 (MQ=255)
                                                                                                                                       aGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCa          <  2:241247/149‑1 (MQ=255)
                                                                                                                                       aGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCa          <  1:2844242/149‑1 (MQ=255)
                                                                                                                                       aGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCa          <  2:3412468/149‑1 (MQ=255)
                                                                                                                                       aGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCa          >  2:3179492/1‑149 (MQ=255)
                                                                                                                                            gCCAAATTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATATGCGGTGCCGGACCAAAGAAATACCAGATTg     >  1:2264791/1‑149 (MQ=255)
                                                                                                                                            gCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTg     >  2:629340/1‑149 (MQ=255)
                                                                                                                                             ccAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGc    <  2:567817/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTATCGAACACTACAGTGTCGAGTGTACTGGCGCGTTGCAGCGCGTCAGCGTCCCGCACCAGCACGCCAAACTCAGCCGCCCGCCCGACGCCGGAAATAATCGACATCGGCGTCGCCAGCCCCAGCGCACACGGACAGGCAATAATCAGTACCGTGGTGGCAATCACCAGGGTATAGACAATCTGCGGTGCCGGACCAAAGAAATACCAGATTGCCG  >  CP009273/505190‑505481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑