BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	601,154	T→A	11.4%	L63F (TTA→TTT)	ybdF ←	DUF419 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	601,154	0	T	A	11.4%	1052.9 / 104.1	308	L63F (TTA→TTT)	ybdF	DUF419 family protein
Reads supporting (aligned to +/- strand): ref base T (165/108); new base A (19/16); total (184/124)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 315 total aligned reads displayed.

TAGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGG  >  CP009273/601006‑601302

     aGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGTTACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAg                                                                                                                                                 <  2:1411164/149‑1 (MQ=255)

        ccATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGa                                                                                                                                              <  2:2735626/149‑1 (MQ=255)

         cATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAt                                                                                                                                             <  2:1640624/149‑1 (MQ=255)

         cATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGAt                                                                                                                                             <  1:3786891/149‑1 (MQ=255)

          aTCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAtt                                                                                                                                            >  2:4061238/1‑149 (MQ=255)

           tCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAttt                                                                                                                                           >  2:3786602/1‑149 (MQ=255)

           tCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAttt                                                                                                                                           >  2:2635010/1‑149 (MQ=255)

                 accaGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTgg                                                                                                                                     <  2:3466738/149‑1 (MQ=255)

                  ccaGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGa                                                                                                                                    <  2:2867586/149‑1 (MQ=255)

                   caGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGAt                                                                                                                                   <  2:17175/149‑1 (MQ=255)

                   caGAGTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCGTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGAt                                                                                                                                   <  2:17109/149‑1 (MQ=255)

                     gATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCg                                                                                                                                 >  1:1353889/1‑149 (MQ=255)

                       ttCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGa                                                                                                                               >  2:2186233/1‑149 (MQ=255)

                       ttCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGa                                                                                                                               <  2:1684803/149‑1 (MQ=255)

                        tCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGAc                                                                                                                              >  2:1352269/1‑149 (MQ=255)

                        tCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGAc                                                                                                                              <  1:5073721/149‑1 (MQ=255)

                        tCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGAc                                                                                                                              <  1:3172420/149‑1 (MQ=255)

                        tCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGAc                                                                                                                              <  1:571259/149‑1 (MQ=255)

                           aCGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTc                                                                                                                           <  1:4918460/149‑1 (MQ=255)

                              aaTCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGa                                                                                                                        >  1:4829043/1‑149 (MQ=255)

                                tCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGAtt                                                                                                                      <  1:1881885/149‑1 (MQ=255)

                                  gTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGTATCGGACTTCAGATTGa                                                                                                                    >  1:1310393/1‑149 (MQ=255)

                                  gTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGa                                                                                                                    >  1:292917/1‑149 (MQ=255)

                                  gTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGa                                                                                                                    >  2:3991910/1‑149 (MQ=255)

                                    tGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATa                                                                                                                  <  2:1286471/149‑1 (MQ=255)

                                       tCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAg                                                                                                               >  1:357621/1‑149 (MQ=255)

                                       tCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAg                                                                                                               >  1:4481850/1‑149 (MQ=255)

                                         aGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAggg                                                                                                             <  2:1128024/149‑1 (MQ=255)

                                          gATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAgggg                                                                                                            >  1:4424963/1‑149 (MQ=255)

                                           aTCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGa                                                                                                           <  2:4302759/149‑1 (MQ=255)

                                           aTCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGa                                                                                                           <  1:4994583/149‑1 (MQ=255)

                                           aTCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTGGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGAGTGATAAAGGGGa                                                                                                           >  2:1990860/1‑149 (MQ=255)

                                                gAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGAcgccg                                                                                                      >  2:2670197/1‑149 (MQ=255)

                                                gAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGAcgccg                                                                                                      >  2:4099388/1‑149 (MQ=255)

                                                  aGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCcgcg                                                                                                    >  2:5158662/1‑149 (MQ=255)

                                                    tAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTa                                                                                                  <  1:2113207/149‑1 (MQ=255)

                                                     aaCGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAg                                                                                                 >  1:3719479/1‑149 (MQ=255)

                                                           tCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCcga                                                                                           <  1:1396004/149‑1 (MQ=255)

                                                              gagaTTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCcgacga                                                                                        >  1:2424493/1‑149 (MQ=255)

                                                              gagaTTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCcgacga                                                                                        >  1:2700918/1‑149 (MQ=255)

                                                                  tttCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGc                                                                                    <  2:4254757/149‑1 (MQ=255)

                                                                   ttcttcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCa                                                                                   <  1:3012741/149‑1 (MQ=255)

                                                                   ttcttcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCa                                                                                   <  1:2613564/149‑1 (MQ=255)

                                                                     cttcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATa                                                                                 <  1:2640027/149‑1 (MQ=255)

                                                                      ttcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATaa                                                                                >  2:2608194/1‑149 (MQ=255)

                                                                       tcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGTGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATaaa                                                                               <  1:1588147/149‑1 (MQ=255)

                                                                        cGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATaaaa                                                                              >  2:4163321/1‑149 (MQ=255)

                                                                           cGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATc                                                                           <  2:4525832/149‑1 (MQ=255)

                                                                            gggATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCt                                                                          <  2:249742/149‑1 (MQ=255)

                                                                             ggATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGTCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCtt                                                                         >  2:3423614/1‑149 (MQ=255)

                                                                                  cacTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTgccgc                                                                    >  1:3365513/1‑149 (MQ=255)

                                                                                   acTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTgccgcc                                                                   <  1:227885/149‑1 (MQ=255)

                                                                                    cTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGAAGCTCCGACGACAGCATAAAAATCTAGCCGCCa                                                                  >  1:3344948/1‑149 (MQ=255)

                                                                                      gAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAt                                                                <  1:747999/149‑1 (MQ=255)

                                                                                       aactCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAtt                                                               >  2:676600/4‑149 (MQ=255)

                                                                                        aaTCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAttt                                                              >  2:4347554/1‑149 (MQ=255)

                                                                                            cAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTaaa                                                          <  2:3165964/149‑1 (MQ=255)

                                                                                            cAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTaaa                                                          <  1:3263808/149‑1 (MQ=255)

                                                                                             aGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTaaaa                                                         >  2:5121023/1‑149 (MQ=255)

                                                                                             aGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTaaaa                                                         >  2:727816/1‑149 (MQ=255)

                                                                                                 cTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAA‑TTTAAAAACATc                                                    <  1:1472021/149‑1 (MQ=255)

                                                                                                  tttttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATc                                                    >  2:3002667/1‑149 (MQ=255)

                                                                                                  tttttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATc                                                    >  2:761415/1‑149 (MQ=255)

                                                                                                     ttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGaa                                                 <  2:233082/149‑1 (MQ=255)

                                                                                                      tATTCATGTGATACCCTGTCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAAc                                                <  2:4575468/149‑1 (MQ=255)

                                                                                                      tATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAAc                                                >  1:1231297/1‑149 (MQ=255)

                                                                                                      tATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAAc                                                >  2:3137698/1‑149 (MQ=255)

                                                                                                       aTTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACt                                               >  2:4985918/1‑149 (MQ=255)

                                                                                                        ttCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTc                                              >  2:4956736/1‑149 (MQ=255)

                                                                                                         tCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTcc                                             >  2:2437788/1‑149 (MQ=255)

                                                                                                              tgATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGcc                                        <  1:3048099/149‑1 (MQ=255)

                                                                                                               gATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCa                                       >  1:3542139/1‑149 (MQ=255)

                                                                                                               gATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCa                                       >  1:4708390/1‑149 (MQ=255)

                                                                                                               gATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCa                                       >  1:543593/1‑149 (MQ=255)

                                                                                                                 tACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCaaa                                     >  1:1006937/1‑149 (MQ=255)

                                                                                                                  aCCCTGGCTTAATGCTTGGTTATATTTGCTTATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCaaaa                                    <  2:5080867/149‑1 (MQ=255)

                                                                                                                    ccTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAgg                                  <  1:1047617/149‑1 (MQ=255)

                                                                                                                      tGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGcc                                >  2:3498767/1‑149 (MQ=255)

                                                                                                                       ggCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCTAACTCCGGGCCAAAAGGCCa                               >  1:1772601/1‑149 (MQ=255)

                                                                                                                       ggCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCa                               >  1:4985223/1‑149 (MQ=255)

                                                                                                                         cTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGc                             >  1:5170929/1‑149 (MQ=255)

                                                                                                                          ttAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCa                            >  1:3897885/1‑149 (MQ=255)

                                                                                                                          ttAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCa                            >  1:5153073/1‑149 (MQ=255)

                                                                                                                            aaTGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCaaa                          <  2:1644655/149‑1 (MQ=255)

                                                                                                                            aaTGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCaaa                          >  2:4763375/1‑149 (MQ=255)

                                                                                                                              tGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGc                        >  2:4376591/1‑149 (MQ=255)

                                                                                                                                    ggTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACa                  >  2:1536890/1‑149 (MQ=255)

                                                                                                                                     gTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACaa                 >  2:2079910/1‑149 (MQ=255)

                                                                                                                                      tataTTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACaaa                <  2:1922434/149‑1 (MQ=255)

                                                                                                                                       atatTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAg               >  1:456556/1‑149 (MQ=255)

                                                                                                                                       atatTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAg               <  2:2306035/149‑1 (MQ=255)

                                                                                                                                        tatTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAgg              <  1:4161434/149‑1 (MQ=255)

                                                                                                                                        tatTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAgg              <  1:1816540/149‑1 (MQ=255)

                                                                                                                                         atTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAggg             <  1:2820636/149‑1 (MQ=255)

                                                                                                                                            ttcttaTTTAACAGGGATTTTTGTGGATCGGACTTCTGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTaa          <  1:891156/144‑1 (MQ=255)

                                                                                                                                             gCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAAc         <  2:3710683/149‑1 (MQ=255)

                                                                                                                                              cTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACt        >  2:2163986/1‑149 (MQ=255)

                                                                                                                                                 aTTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCaa     >  2:1743253/1‑149 (MQ=255)

                                                                                                                                                 aTTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCaa     >  1:196870/1‑149 (MQ=255)

                                                                                                                                                   ttAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAgg   >  1:975887/1‑149 (MQ=255)

TAGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGG  >  CP009273/601006‑601302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`