Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,071,619 G→A 7.1% A907V (GCC→GTC)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,071,6190GA 7.1% 993.4 / 42.6 281A907V (GCC→GTC) putAfused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (115/146);  new base A (4/16);  total (119/162)
Fisher's exact test for biased strand distribution p-value = 3.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

GATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACGTAGCGACTTC  >  CP009273/1071471‑1071767
                                                                                                                                                    |                                                                                                                                                    
       gtggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTAc                                                                                                                                               <  2:825476/149‑1 (MQ=255)
       gtggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTAc                                                                                                                                               <  2:4171246/149‑1 (MQ=255)
       gtggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTAc                                                                                                                                               <  1:2632608/149‑1 (MQ=255)
        tggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACa                                                                                                                                              >  1:825661/1‑149 (MQ=255)
         ggtCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACAt                                                                                                                                             >  1:3650121/1‑149 (MQ=255)
              ggCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCacg                                                                                                                                        >  1:3509259/1‑149 (MQ=255)
               gCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCacga                                                                                                                                       >  1:318568/1‑149 (MQ=255)
                cGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGACCAGTACATCCacgac                                                                                                                                      <  1:1550190/149‑1 (MQ=255)
                  aCCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCAcgacga                                                                                                                                    <  1:1124483/149‑1 (MQ=255)
                  aCCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCAcgacga                                                                                                                                    >  2:1684462/1‑149 (MQ=255)
                  aCCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCAcgacga                                                                                                                                    <  1:3721332/149‑1 (MQ=255)
                  aCCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCAcgacga                                                                                                                                    >  2:4740390/1‑149 (MQ=255)
                  aCCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCAcgacga                                                                                                                                    >  2:5103091/1‑149 (MQ=255)
                   cccGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACgacgac                                                                                                                                   <  1:3384028/149‑1 (MQ=255)
                       gATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGt                                                                                                                               <  2:4538092/149‑1 (MQ=255)
                       gATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGt                                                                                                                               <  1:2059846/149‑1 (MQ=255)
                         ttACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTc                                                                                                                             >  1:934798/1‑149 (MQ=255)
                         ttACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTc                                                                                                                             >  2:4039864/1‑149 (MQ=255)
                         ttACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTc                                                                                                                             >  2:2893326/1‑149 (MQ=255)
                           aCCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCgg                                                                                                                           <  2:4992371/149‑1 (MQ=255)
                            cccATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGt                                                                                                                          <  2:1202769/149‑1 (MQ=255)
                              cATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCa                                                                                                                        <  1:2122395/149‑1 (MQ=255)
                               aTCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAg                                                                                                                       >  1:3326336/1‑149 (MQ=255)
                                tCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGt                                                                                                                      <  2:293041/149‑1 (MQ=255)
                                tCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGt                                                                                                                      <  1:3768276/149‑1 (MQ=255)
                                  cGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGc                                                                                                                    <  1:4689139/149‑1 (MQ=255)
                                   ggCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCTCTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCt                                                                                                                   <  1:1122675/149‑1 (MQ=255)
                                    gCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCGTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTg                                                                                                                  <  2:3964353/149‑1 (MQ=255)
                                        tCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTgaaga                                                                                                              <  2:2671293/149‑1 (MQ=255)
                                         cGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTgaagaa                                                                                                             <  2:1522696/149‑1 (MQ=255)
                                         cGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTgaagaa                                                                                                             <  1:1896707/149‑1 (MQ=255)
                                           gCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATc                                                                                                           <  2:4416933/149‑1 (MQ=255)
                                              aTTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGAc                                                                                                        <  2:1290543/149‑1 (MQ=255)
                                              aTTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGAc                                                                                                        <  2:2278102/149‑1 (MQ=255)
                                                tGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACaa                                                                                                      <  2:2008000/149‑1 (MQ=255)
                                                            caTTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCa                                                                                          <  1:4314154/149‑1 (MQ=255)
                                                            caTTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGACCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCa                                                                                          <  1:4958543/149‑1 (MQ=255)
                                                            caTTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGACCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCa                                                                                          <  1:5033062/149‑1 (MQ=255)
                                                            caTTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGACACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCa                                                                                          <  2:4236109/149‑1 (MQ=255)
                                                              ttttCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATg                                                                                        >  2:2788115/1‑149 (MQ=255)
                                                               tttCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATgc                                                                                       <  2:3334781/149‑1 (MQ=255)
                                                               tttCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATgc                                                                                       >  2:3619831/1‑149 (MQ=255)
                                                               tttCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATgc                                                                                       >  2:3524428/1‑149 (MQ=255)
                                                                ttCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATgcc                                                                                      >  1:2448303/1‑149 (MQ=255)
                                                                ttCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATgcc                                                                                      <  1:1305205/149‑1 (MQ=255)
                                                                  cAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATgccgc                                                                                    >  1:3105014/1‑149 (MQ=255)
                                                                  cAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATgccgc                                                                                    <  1:977780/149‑1 (MQ=255)
                                                                        gtgGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGttt                                                                              <  1:4260699/149‑1 (MQ=255)
                                                                         tgGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTc                                                                             <  1:2247495/149‑1 (MQ=255)
                                                                         tgGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTc                                                                             <  1:1180760/149‑1 (MQ=255)
                                                                         tgGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTc                                                                             <  1:2490652/149‑1 (MQ=255)
                                                                            tCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGc                                                                          >  2:2108568/1‑149 (MQ=255)
                                                                            tCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGc                                                                          <  1:5049072/149‑1 (MQ=255)
                                                                                   tctcATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGc                                                                   <  2:3689613/149‑1 (MQ=255)
                                                                                   tctcATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGc                                                                   >  2:736987/1‑149 (MQ=255)
                                                                                   tctcATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGc                                                                   >  1:4045723/1‑149 (MQ=255)
                                                                                    ctcATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCg                                                                  >  1:951010/1‑149 (MQ=255)
                                                                                      cATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGa                                                                <  1:3325239/149‑1 (MQ=255)
                                                                                        tCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAAt                                                              <  2:570374/149‑1 (MQ=255)
                                                                                        tCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAAt                                                              >  1:4053282/1‑149 (MQ=255)
                                                                                          ttGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAg                                                            <  2:1452532/149‑1 (MQ=255)
                                                                                          ttGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGACCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAg                                                            >  1:428674/1‑149 (MQ=255)
                                                                                           tGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAgg                                                           <  1:2160474/149‑1 (MQ=255)
                                                                                            gCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAggg                                                          <  1:3524428/149‑1 (MQ=255)
                                                                                            gCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAggg                                                          <  2:1373444/149‑1 (MQ=255)
                                                                                                    aGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTg                                                  <  1:1898314/149‑1 (MQ=255)
                                                                                                    aGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTg                                                  >  2:2730421/1‑149 (MQ=255)
                                                                                                    aGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTg                                                  <  1:1650590/149‑1 (MQ=255)
                                                                                                         gcgcAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGt                                             >  1:660593/1‑149 (MQ=255)
                                                                                                         gcgcAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGt                                             <  1:2729960/149‑1 (MQ=255)
                                                                                                           gcAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTcc                                           <  2:2074830/149‑1 (MQ=255)
                                                                                                            cAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCa                                          >  1:769318/1‑149 (MQ=255)
                                                                                                                  cGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAggcggc                                    >  2:1026660/1‑149 (MQ=255)
                                                                                                                  cGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAggcggc                                    <  1:3886693/149‑1 (MQ=255)
                                                                                                                   gAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCt                                   <  1:2883975/149‑1 (MQ=255)
                                                                                                                   gAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCt                                   <  2:3893990/149‑1 (MQ=255)
                                                                                                                    aacaacGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTg                                  >  1:2224277/1‑149 (MQ=255)
                                                                                                                    aacaacGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTg                                  <  1:4740390/149‑1 (MQ=255)
                                                                                                                     acaacGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTgg                                 >  2:622629/1‑149 (MQ=255)
                                                                                                                      caacGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGc                                >  1:4572132/1‑149 (MQ=255)
                                                                                                                           cTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGatat                           <  1:4789791/149‑1 (MQ=255)
                                                                                                                            tGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATAtt                          >  1:2432073/1‑149 (MQ=255)
                                                                                                                              aCCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTgc                        <  2:1877066/149‑1 (MQ=255)
                                                                                                                              aCCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTgc                        >  2:902774/1‑149 (MQ=255)
                                                                                                                                  gCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTg                    >  2:2526254/1‑149 (MQ=255)
                                                                                                                                     cTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAg                 >  2:49959/1‑149 (MQ=255)
                                                                                                                                     cTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAg                 <  2:318568/149‑1 (MQ=255)
                                                                                                                                      tGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGt                <  1:3031538/149‑1 (MQ=255)
                                                                                                                                      tGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGt                <  1:4897345/149‑1 (MQ=255)
                                                                                                                                      tGTCGAACGCCGAGACCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGt                <  2:5009907/149‑1 (MQ=255)
                                                                                                                                         cGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAAc             <  2:3509259/149‑1 (MQ=255)
                                                                                                                                          gAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACg            >  2:4004773/1‑149 (MQ=255)
                                                                                                                                          gAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACg            >  1:527190/1‑149 (MQ=255)
                                                                                                                                          gAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACg            >  2:1126854/1‑149 (MQ=255)
                                                                                                                                           aaCGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACGt           <  1:2772584/149‑1 (MQ=255)
                                                                                                                                                  aGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACGTAGCGACt    >  1:4526805/1‑149 (MQ=255)
                                                                                                                                                  aGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACGTAGCGACt    >  1:3088342/1‑149 (MQ=255)
                                                                                                                                                  aGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACGTAGCGACt    <  1:1232143/149‑1 (MQ=255)
                                                                                                                                                   ggCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACGTAGCGACtt   <  1:3608098/149‑1 (MQ=255)
                                                                                                                                                    gCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACGTAGCGACTTc  >  2:1760117/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATATCGGTGGTCAGGCGACCCGGATTACCCATCCGGCATTCGGCCATTGCGCCGCGCAGCATTTTCAACGTGTGGTCGGCAATCTCATCTTGCAGGCACAGCACGCGCAGCGCCGAACAACGCTGACCCGCACTGTCGAACGCCGAGGCCAGTACATCCACGACGACCTGTTCGGTCAGTGCTGAAGAATCGACAATCATCGCGTTCATGCCGCCGGTTTCAGCGATGAGCGGAATAGGGCGACCCTGAGCGTCCAGGCGGCTGGCGATATTGCGCTGCAGTAACGTAGCGACTTC  >  CP009273/1071471‑1071767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑