BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,129,182	G→A	7.9%	A385T (GCC→ACC)	flgE →	flagellar hook protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,129,182	0	G	A	7.9%	1008.4 / 51.6	293	A385T (GCC→ACC)	flgE	flagellar hook protein
Reads supporting (aligned to +/- strand): ref base G (137/132); new base A (11/12); total (149/144)
Fisher's exact test for biased strand distribution p-value = 8.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 297 total aligned reads displayed.

GACGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTA  >  CP009273/1129034‑1129330

   gCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGccc                                                                                                                                                   >  2:184884/1‑149 (MQ=255)

   gCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGccc                                                                                                                                                   >  2:3806157/1‑149 (MQ=255)

     aaTCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAg                                                                                                                                                 >  2:2112931/1‑149 (MQ=255)

     aaTCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAg                                                                                                                                                 >  2:266461/1‑149 (MQ=255)

       tcttctGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGAc                                                                                                                                               <  1:1586365/149‑1 (MQ=255)

       tcttctGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGAc                                                                                                                                               >  2:1953161/1‑149 (MQ=255)

       tcttctGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGAc                                                                                                                                               <  1:5148336/149‑1 (MQ=255)

         ttctGGCGTGGCGCTGTTGGGGACGGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGAccc                                                                                                                                             >  2:2437182/1‑148 (MQ=255)

          tctGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCAt                                                                                                                                            <  1:1258160/149‑1 (MQ=255)

                gtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAc                                                                                                                                      <  2:4215140/149‑1 (MQ=255)

                  ggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAccc                                                                                                                                    <  2:2521142/149‑1 (MQ=255)

                   gcgcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCa                                                                                                                                   <  1:612599/149‑1 (MQ=255)

                   gcgcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCa                                                                                                                                   >  1:58496/1‑149 (MQ=255)

                    cgcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAg                                                                                                                                  <  2:3117668/149‑1 (MQ=255)

                     gcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAgg                                                                                                                                 >  2:1250123/1‑149 (MQ=255)

                         ttGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCa                                                                                                                             <  1:4016498/149‑1 (MQ=255)

                         ttGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCa                                                                                                                             >  2:3607417/1‑149 (MQ=255)

                           ggggACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGa                                                                                                                           >  1:5030785/1‑149 (MQ=255)

                            gggACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGAt                                                                                                                          <  1:2639042/149‑1 (MQ=255)

                             ggACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATc                                                                                                                         <  2:3926529/149‑1 (MQ=255)

                               aCAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCt                                                                                                                       <  2:1169693/149‑1 (MQ=255)

                               aCAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCt                                                                                                                       <  2:5047577/149‑1 (MQ=255)

                               aCAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCt                                                                                                                       <  2:3341056/149‑1 (MQ=255)

                                cAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTc                                                                                                                      <  1:1193366/149‑1 (MQ=255)

                                     gggacgggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACCCCCAGACCCTCATAACCCAGGCCCCGATCCTCAacac                                                                                                                 >  1:2699191/1‑149 (MQ=255)

                                       gacgggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGc                                                                                                               >  2:1775563/1‑149 (MQ=255)

                                       gacgggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGc                                                                                                               <  2:4133159/149‑1 (MQ=255)

                                        acgggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCt                                                                                                              <  2:3492317/149‑1 (MQ=255)

                                         cgggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTg                                                                                                             <  1:4896592/149‑1 (MQ=255)

                                          gggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTgg                                                                                                            <  1:4419843/149‑1 (MQ=255)

                                          gggaAACTTTGGCACCCTGACAATCGGTGCGCTGTAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTgg                                                                                                            <  1:1824574/149‑1 (MQ=255)

                                           ggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGt                                                                                                           <  1:2160755/149‑1 (MQ=255)

                                           ggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGt                                                                                                           >  2:1538528/1‑149 (MQ=255)

                                           ggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGt                                                                                                           <  2:4349046/149‑1 (MQ=255)

                                           ggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGt                                                                                                           <  1:4965830/149‑1 (MQ=255)

                                              aaCTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTaa                                                                                                        >  1:1142363/1‑149 (MQ=255)

                                               aCTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAAc                                                                                                       <  1:2547175/149‑1 (MQ=255)

                                                 tttGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACtt                                                                                                     >  1:403593/1‑149 (MQ=255)

                                                  ttGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTa                                                                                                    <  1:2115726/149‑1 (MQ=255)

                                                  ttGGCACACTGACCAACGGTGCGCTGGAAGCGTCCAACGTCTATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTa                                                                                                    <  1:3330552/149‑1 (MQ=255)

                                                   tGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTAc                                                                                                   >  2:835766/1‑149 (MQ=255)

                                                      cACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCt                                                                                                >  1:899435/1‑149 (MQ=255)

                                                      cACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCt                                                                                                <  1:4842960/149‑1 (MQ=255)

                                                      cACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCt                                                                                                <  2:2292638/149‑1 (MQ=255)

                                                        cccTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTaa                                                                                              <  2:2705618/149‑1 (MQ=255)

                                                        cccTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTaa                                                                                              <  1:4599460/149‑1 (MQ=255)

                                                        cccTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTaa                                                                                              >  2:579964/1‑149 (MQ=255)

                                                          cTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATc                                                                                            >  2:1720594/1‑149 (MQ=255)

                                                            gACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGc                                                                                          <  2:889556/149‑1 (MQ=255)

                                                            gACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGc                                                                                          <  2:254209/149‑1 (MQ=255)

                                                               cAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGa                                                                                       <  1:184884/149‑1 (MQ=255)

                                                               cAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGa                                                                                       <  2:1562699/149‑1 (MQ=255)

                                                               cAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGa                                                                                       <  1:317866/149‑1 (MQ=255)

                                                                    gTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGa                                                                                  >  1:5012962/1‑149 (MQ=255)

                                                                    gTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGa                                                                                  >  1:3525756/1‑149 (MQ=255)

                                                                      gcgcTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATa                                                                                <  2:937639/149‑1 (MQ=255)

                                                                         cTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCt                                                                             <  1:1415277/149‑1 (MQ=255)

                                                                            gAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCaa                                                                          <  1:987893/149‑1 (MQ=255)

                                                                              aGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATg                                                                        >  1:2006871/1‑149 (MQ=255)

                                                                              aGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATg                                                                        <  2:2963299/149‑1 (MQ=255)

                                                                              aGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATg                                                                        <  2:3402984/149‑1 (MQ=255)

                                                                                 gTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGAt                                                                     >  1:1955062/1‑149 (MQ=255)

                                                                                 gTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGAt                                                                     <  2:1072829/149‑1 (MQ=255)

                                                                                  tCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATc                                                                    <  1:1171513/149‑1 (MQ=255)

                                                                                  tCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATc                                                                    >  2:3479243/1‑149 (MQ=255)

                                                                                    cAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCAc                                                                  >  1:895856/1‑149 (MQ=255)

                                                                                          cGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAAtt                                                            >  1:5041874/1‑149 (MQ=255)

                                                                                            aTCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCACTTta                                                          >  2:4803815/1‑149 (MQ=255)

                                                                                            aTCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTta                                                          >  1:4451364/1‑149 (MQ=255)

                                                                                                  gTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACcgc                                                    <  1:2279936/149‑1 (MQ=255)

                                                                                                  gTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACcgc                                                    >  1:935392/1‑149 (MQ=255)

                                                                                                     aaGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGAt                                                 <  1:1550565/149‑1 (MQ=255)

                                                                                                      aGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACTCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATg                                                >  1:2983449/1‑149 (MQ=255)

                                                                                                        aaCTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATggg                                              <  1:4721635/149‑1 (MQ=255)

                                                                                                         aCTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGa                                             <  1:4796335/149‑1 (MQ=255)

                                                                                                          cTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGag                                            >  2:2022105/1‑149 (MQ=255)

                                                                                                            ggTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGagca                                          <  1:3794555/149‑1 (MQ=255)

                                                                                                                 atatGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGcagccag                                     >  2:1470421/1‑149 (MQ=255)

                                                                                                                  tatGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGcagccagc                                    >  1:4379680/1‑149 (MQ=255)

                                                                                                                    tGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAgccagcca                                  >  2:2470504/1‑149 (MQ=255)

                                                                                                                      aTCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGa                                <  2:3073201/149‑1 (MQ=255)

                                                                                                                      aTCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGa                                >  2:668122/1‑149 (MQ=255)

                                                                                                                          ttGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACt                            >  1:1009147/1‑149 (MQ=255)

                                                                                                                           tGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTg                           >  1:1479048/1‑149 (MQ=255)

                                                                                                                            gCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGa                          >  1:3371179/1‑149 (MQ=255)

                                                                                                                              ccAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAAt                        <  2:3178756/149‑1 (MQ=255)

                                                                                                                              ccAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAAt                        <  2:4945903/149‑1 (MQ=255)

                                                                                                                                 gCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCaa                     <  2:4626331/149‑1 (MQ=255)

                                                                                                                                    tAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAg                  <  2:3958422/149‑1 (MQ=255)

                                                                                                                                     aaCTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAgg                 >  2:4928201/1‑149 (MQ=255)

                                                                                                                                     aaCTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAgg                 >  1:3528026/1‑149 (MQ=255)

                                                                                                                                     aaCTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAgg                 >  2:4348614/1‑149 (MQ=255)

                                                                                                                                      aCTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTGATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGc                >  2:3990203/1‑149 (MQ=255)

                                                                                                                                      aCTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGc                >  1:3877991/1‑149 (MQ=255)

                                                                                                                                          tCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTa            >  2:1837051/1‑149 (MQ=255)

                                                                                                                                          tCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTa            >  2:250317/1‑149 (MQ=255)

                                                                                                                                           cAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTaa           <  2:5191470/149‑1 (MQ=255)

                                                                                                                                                tAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGcc      >  1:173751/1‑149 (MQ=255)

                                                                                                                                                 aaCGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCa     >  2:2603850/1‑149 (MQ=255)

                                                                                                                                                    gCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTa  >  2:4592778/1‑149 (MQ=255)

GACGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTA  >  CP009273/1129034‑1129330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`