BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,152,278	G→T	43.1%	E16* (GAA→TAA)	ycfH →	putative DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,152,278	0	G	T	43.1%	210.6 / inf	255	E16* (GAA→TAA)	ycfH	putative DNase
Reads supporting (aligned to +/- strand): ref base G (81/64); new base T (61/49); total (142/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 257 total aligned reads displayed.

CATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTG  >  CP009273/1152132‑1152425

cATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGaa                                                                                                                                                   >  2:45601/1‑149 (MQ=255)

  tCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGGTCCTCATCTTTAAGAGAGACATCATGTGTTTAGTCGACTCACACGGCCCTCTCGATGGTCTGGATTATTAATc                                                                                                                                                 >  2:2448227/1‑149 (MQ=255)

    aaCCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACCTCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCtt                                                                                                                                               >  1:3626393/1‑149 (MQ=255)

    aaCCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCtt                                                                                                                                               <  1:1181449/149‑1 (MQ=255)

    aaCCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCtt                                                                                                                                               >  2:403197/1‑149 (MQ=255)

     aCCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCttt                                                                                                                                              <  1:3481956/149‑1 (MQ=255)

      ccGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTg                                                                                                                                             <  1:813226/149‑1 (MQ=255)

      ccGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTg                                                                                                                                             >  2:81826/1‑149 (MQ=255)

           aGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATaa                                                                                                                                        <  2:1612513/149‑1 (MQ=255)

              ttCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGa                                                                                                                                     >  2:2107070/1‑149 (MQ=255)

               tctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGAc                                                                                                                                    >  1:1602310/1‑149 (MQ=255)

               tctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGAc                                                                                                                                    >  1:1276739/1‑149 (MQ=255)

                 tcatcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGt                                                                                                                                  >  1:936013/1‑149 (MQ=255)

                  catcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTg                                                                                                                                 >  1:4905634/1‑149 (MQ=255)

                  catcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTg                                                                                                                                 <  1:2156692/149‑1 (MQ=255)

                      aCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATg                                                                                                                             >  1:4526966/1‑149 (MQ=255)

                       ccGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGa                                                                                                                            <  2:4124862/149‑1 (MQ=255)

                         gATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACg                                                                                                                          >  1:3159737/1‑149 (MQ=255)

                           tCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGtt                                                                                                                        <  2:882921/149‑1 (MQ=255)

                            cTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTc                                                                                                                       >  2:3548540/1‑149 (MQ=255)

                            cTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTc                                                                                                                       >  2:348013/1‑149 (MQ=255)

                            cTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTc                                                                                                                       >  2:3995725/1‑149 (MQ=255)

                              tttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTg                                                                                                                     <  2:1884903/149‑1 (MQ=255)

                               ttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTgg                                                                                                                    <  2:3198103/149‑1 (MQ=255)

                               ttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTgg                                                                                                                    <  1:1371880/149‑1 (MQ=255)

                                 aCTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCg                                                                                                                  >  2:3957138/1‑149 (MQ=255)

                                      gTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgc                                                                                                             >  1:5035897/1‑149 (MQ=255)

                                      gTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgc                                                                                                             >  1:3695381/1‑149 (MQ=255)

                                       tATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAgcc                                                                                                            >  1:3302682/1‑149 (MQ=255)

                                           gAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGccgccg                                                                                                        >  2:1645462/1‑149 (MQ=255)

                                              cATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCAc                                                                                                     <  1:2975406/149‑1 (MQ=255)

                                               aTTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCAcg                                                                                                    >  1:4266152/1‑149 (MQ=255)

                                                 tACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCAcgcg                                                                                                  >  1:1141311/1‑149 (MQ=255)

                                                   ccTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAt                                                                                                <  2:737354/149‑1 (MQ=255)

                                                      gCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTCAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCAGCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGAtgtg                                                                                             <  2:5073406/149‑1 (MQ=255)

                                                         aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaaa                                                                                          >  1:4748678/1‑149 (MQ=255)

                                                         aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaaa                                                                                          >  1:2584882/1‑149 (MQ=255)

                                                           cGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAtt                                                                                        <  2:390870/149‑1 (MQ=255)

                                                             ggCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAtttt                                                                                      >  1:2441733/1‑149 (MQ=255)

                                                              gCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTg                                                                                     >  2:2995944/1‑149 (MQ=255)

                                                                 ttGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCt                                                                                  <  1:4365832/149‑1 (MQ=255)

                                                                 ttGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCt                                                                                  >  2:4341865/1‑149 (MQ=255)

                                                                      cTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAg                                                                             <  1:3541930/149‑1 (MQ=255)

                                                                      cTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAg                                                                             >  2:2591693/1‑149 (MQ=255)

                                                                        aCCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTc                                                                           >  1:3933844/1‑149 (MQ=255)

                                                                          cGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTctc                                                                         >  1:3363235/1‑147 (MQ=255)

                                                                          cGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGc                                                                         >  1:3027618/1‑149 (MQ=255)

                                                                            gTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCca                                                                       <  1:3979213/149‑1 (MQ=255)

                                                                             ttCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCcac                                                                      >  2:1302340/1‑149 (MQ=255)

                                                                               ccTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaa                                                                    <  2:617888/149‑1 (MQ=255)

                                                                                  cATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACGGCCATCTCGATGGTCTGGATTATGAATCTTGGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACAt                                                                 <  1:3152237/149‑1 (MQ=255)

                                                                                   aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACAtt                                                                <  1:563036/149‑1 (MQ=255)

                                                                                   aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACAtt                                                                >  2:3242303/1‑149 (MQ=255)

                                                                                   aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACAtt                                                                >  1:490800/1‑149 (MQ=255)

                                                                                   aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCACCACAACAtt                                                                >  1:4867402/1‑149 (MQ=255)

                                                                                     cTTTTAGAGAGAAATCTTGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGAATAAGTTAGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTAc                                                              <  1:1002406/149‑1 (MQ=255)

                                                                                     cTTTCAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTAc                                                              <  2:2373516/149‑1 (MQ=255)

                                                                                       ttAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCg                                                            <  1:1978813/149‑1 (MQ=255)

                                                                                        tAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCgg                                                           <  2:3611700/149‑1 (MQ=255)

                                                                                        tAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCgg                                                           <  2:3663231/149‑1 (MQ=255)

                                                                                        tAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTGGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCgg                                                           <  2:3693483/149‑1 (MQ=255)

                                                                                         aaGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCggg                                                          <  2:215788/149‑1 (MQ=255)

                                                                                          agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGt                                                         >  2:4439641/1‑149 (MQ=255)

                                                                                          agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGt                                                         <  1:4778415/149‑1 (MQ=255)

                                                                                           gagagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                        >  1:3818232/1‑149 (MQ=255)

                                                                                              agaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAtt                                                     >  1:1695133/1‑149 (MQ=255)

                                                                                               gaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAttt                                                    <  2:2039856/149‑1 (MQ=255)

                                                                                                  atcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACa                                                 <  2:4938622/149‑1 (MQ=255)

                                                                                                   tcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACat                                                >  1:2732879/1‑149 (MQ=255)

                                                                                                    catGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACata                                               <  2:2036668/149‑1 (MQ=255)

                                                                                                         ttttAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCgg                                          <  1:4996565/149‑1 (MQ=255)

                                                                                                          tttAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCggg                                         <  2:4759383/149‑1 (MQ=255)

                                                                                                             aGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATc                                      <  1:552519/149‑1 (MQ=255)

                                                                                                                cGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTgg                                   <  1:242471/149‑1 (MQ=255)

                                                                                                                 gACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGt                                  <  2:4385355/149‑1 (MQ=255)

                                                                                                                  aCTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTa                                 >  2:2375771/1‑149 (MQ=255)

                                                                                                                    tCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGCCATCTGGTAgg                               >  1:1324963/1‑149 (MQ=255)

                                                                                                                      acacTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCg                             <  2:4905634/149‑1 (MQ=255)

                                                                                                                      acacTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCg                             >  2:1932354/1‑149 (MQ=255)

                                                                                                                      acacTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCg                             >  1:2754440/1‑149 (MQ=255)

                                                                                                                          tGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACg                         >  2:1017046/1‑149 (MQ=255)

                                                                                                                          tGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACg                         >  2:696244/1‑149 (MQ=255)

                                                                                                                           gCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGt                        >  1:2362702/1‑149 (MQ=255)

                                                                                                                            ccATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTg                       >  1:2488890/1‑149 (MQ=255)

                                                                                                                            ccATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTg                       <  2:2685512/149‑1 (MQ=255)

                                                                                                                                   gATGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgt                <  1:4591402/149‑1 (MQ=255)

                                                                                                                                    aTGGTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtc               <  2:4630768/149‑1 (MQ=255)

                                                                                                                                    aTGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtc               <  1:2591693/149‑1 (MQ=255)

                                                                                                                                    aTGGTCTGGAGTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtc               <  2:4407534/149‑1 (MQ=255)

                                                                                                                                       gTCTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTa            <  1:3136359/149‑1 (MQ=255)

                                                                                                                                       gTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTa            >  1:3586809/1‑149 (MQ=255)

                                                                                                                                       gTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTa            <  2:1141685/149‑1 (MQ=255)

                                                                                                                                         cTGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAtt          <  1:1083019/149‑1 (MQ=255)

                                                                                                                                         cTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAtt          <  2:1768528/149‑1 (MQ=255)

                                                                                                                                          tGGATTATTAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAttt         >  1:3667365/1‑149 (MQ=255)

                                                                                                                                          tGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAttt         >  2:4343708/1‑149 (MQ=255)

                                                                                                                                            gATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTc       <  1:1370176/149‑1 (MQ=255)

                                                                                                                                            gATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTc       >  2:2841351/1‑149 (MQ=255)

                                                                                                                                             aTTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCt      <  1:4341865/149‑1 (MQ=255)

                                                                                                                                             aTTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCt      >  2:4792492/1‑149 (MQ=255)

CATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTG  >  CP009273/1152132‑1152425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`