Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,229,460 G→T 12.0% F238L (TTC→TTA)  nhaB ← sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,229,4600GT12.0% 1092.5 / 95.5 309F238L (TTC→TTA) nhaBsodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (129/143);  new base T (22/15);  total (151/158)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 317 total aligned reads displayed.

CTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTT  >  CP009273/1229314‑1229608
                                                                                                                                                  |                                                                                                                                                    
 tttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGaaa                                                                                                                                                   <  2:733656/149‑1 (MQ=255)
  ttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCCACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAAt                                                                                                                                                  <  1:1117656/149‑1 (MQ=255)
   tGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATc                                                                                                                                                 >  1:2981143/1‑149 (MQ=255)
    gATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCg                                                                                                                                                <  2:3277142/149‑1 (MQ=255)
        gTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCaa                                                                                                                                            >  2:2966701/1‑149 (MQ=255)
        gTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCaa                                                                                                                                            <  2:3439537/149‑1 (MQ=255)
          cAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCaaaa                                                                                                                                          <  1:2490186/149‑1 (MQ=255)
           aaaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACCTGCGCAGGAAGAAATCGCCAAAAt                                                                                                                                         >  2:657965/1‑149 (MQ=255)
           aaaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAAt                                                                                                                                         <  1:4870390/149‑1 (MQ=255)
             aCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgc                                                                                                                                       >  1:4175577/1‑149 (MQ=255)
             aCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgc                                                                                                                                       >  1:1203866/1‑149 (MQ=255)
              cTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgcc                                                                                                                                      >  2:4003047/1‑149 (MQ=255)
               tGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgcca                                                                                                                                     >  2:826709/1‑149 (MQ=255)
               tGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgcca                                                                                                                                     >  2:3225274/1‑149 (MQ=255)
                 ttGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACCTGCGCAGGAAGAAATCGCCAAAATgccagc                                                                                                                                   >  2:158575/1‑149 (MQ=255)
                  tGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccagcc                                                                                                                                  <  2:2003576/149‑1 (MQ=255)
                    cAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGccagccag                                                                                                                                >  1:2096702/1‑149 (MQ=255)
                      acacTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCagccagcc                                                                                                                              >  2:1863395/1‑149 (MQ=255)
                        acTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGc                                                                                                                            >  2:1710729/1‑149 (MQ=255)
                        acTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGc                                                                                                                            >  2:4840378/1‑149 (MQ=255)
                        acTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGc                                                                                                                            >  2:874173/1‑149 (MQ=255)
                         cTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCt                                                                                                                           <  1:4520520/149‑1 (MQ=255)
                          ttCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCtt                                                                                                                          >  1:2016302/1‑149 (MQ=255)
                           tCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCttt                                                                                                                         <  2:4498506/149‑1 (MQ=255)
                            cgcgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTa                                                                                                                        <  2:2262342/149‑1 (MQ=255)
                             gcgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAg                                                                                                                       <  2:1743175/149‑1 (MQ=255)
                                gACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACAGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCga                                                                                                                    >  2:3481562/1‑149 (MQ=255)
                                  cTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatg                                                                                                                  <  2:2347529/149‑1 (MQ=255)
                                   tttCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatga                                                                                                                 >  1:763053/1‑149 (MQ=255)
                                    ttCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatgat                                                                                                                <  2:5082794/149‑1 (MQ=255)
                                     tctcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATc                                                                                                               <  2:395750/149‑1 (MQ=255)
                                      ctcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCa                                                                                                              <  1:584275/149‑1 (MQ=255)
                                      ctcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCa                                                                                                              >  2:1629572/1‑149 (MQ=255)
                                         cGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGt                                                                                                           <  2:1671742/149‑1 (MQ=255)
                                         cGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGt                                                                                                           <  2:4624374/149‑1 (MQ=255)
                                          ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGACCGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                          >  1:355670/1‑149 (MQ=255)
                                           gCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACCTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCGTTAGCGATGATCAGGTTc                                                                                                         >  2:315001/1‑149 (MQ=255)
                                                gTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACGAGCTGGCAGGTTAACAGGCCTCAAATCAGACCCTGATAGGTCACCTGCGACCTGCGCATGAAGATATCGCGAACACCCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTgg                                                                                                    <  1:998122/149‑1 (MQ=255)
                                                   tCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTc                                                                                                 >  1:2468778/1‑149 (MQ=255)
                                                   tCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTc                                                                                                 <  1:1697898/149‑1 (MQ=255)
                                                    cACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCg                                                                                                >  1:4129870/1‑149 (MQ=255)
                                                     aCCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGc                                                                                               >  2:427825/1‑149 (MQ=255)
                                                     aCCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGc                                                                                               >  2:3028472/1‑149 (MQ=255)
                                                            ccAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCAt                                                                                        <  1:4607311/149‑1 (MQ=255)
                                                              aaaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGACGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATgg                                                                                      >  2:3318897/1‑149 (MQ=255)
                                                              aaaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATgg                                                                                      >  2:1080148/1‑149 (MQ=255)
                                                               aaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGt                                                                                     >  1:4064005/1‑149 (MQ=255)
                                                                aCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTc                                                                                    <  2:4359363/149‑1 (MQ=255)
                                                                   aaCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAtt                                                                                 <  1:3891430/149‑1 (MQ=255)
                                                                   aaCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAtt                                                                                 <  1:3510204/149‑1 (MQ=255)
                                                                   aaCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAtt                                                                                 >  2:30733/1‑149 (MQ=255)
                                                                   aaCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAtt                                                                                 <  1:469165/149‑1 (MQ=255)
                                                                    aCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTa                                                                                <  2:1904380/149‑1 (MQ=255)
                                                                    aCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTa                                                                                >  2:1275396/1‑149 (MQ=255)
                                                                       cAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGACCCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgc                                                                             <  2:577329/149‑1 (MQ=255)
                                                                       cAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgc                                                                             >  2:1938847/1‑149 (MQ=255)
                                                                         gCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgccg                                                                           >  1:2627574/1‑149 (MQ=255)
                                                                         gCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgccg                                                                           >  2:69795/1‑149 (MQ=255)
                                                                           ttCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACgccgcc                                                                         >  2:1388073/1‑149 (MQ=255)
                                                                            tctcTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCt                                                                        >  2:2527070/1‑149 (MQ=255)
                                                                              tctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTaa                                                                      <  2:2088032/149‑1 (MQ=255)
                                                                              tctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTaa                                                                      <  2:1798654/149‑1 (MQ=255)
                                                                                 aCCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGc                                                                   >  2:4760161/1‑149 (MQ=255)
                                                                                   cagcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCgg                                                                 <  1:3797510/149‑1 (MQ=255)
                                                                                       aggcaggTTACCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGACATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCAGGGTCATTACGCCGCCTAATGCGGTGcc                                                             >  2:389032/1‑149 (MQ=255)
                                                                                       aggcaggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGcc                                                             >  2:3621460/1‑149 (MQ=255)
                                                                                           aggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGaca                                                         >  2:2517030/1‑149 (MQ=255)
                                                                                           aggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGaca                                                         >  1:883600/1‑149 (MQ=255)
                                                                                              ttAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCg                                                      <  1:1591112/149‑1 (MQ=255)
                                                                                               tAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCgg                                                     <  1:2814465/149‑1 (MQ=255)
                                                                                                 aCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgca                                                   <  1:3104869/149‑1 (MQ=255)
                                                                                                  cAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgcat                                                  <  1:4756917/149‑1 (MQ=255)
                                                                                                        acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatca                                            >  2:3987024/1‑149 (MQ=255)
                                                                                                        acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatca                                            <  1:512490/149‑1 (MQ=255)
                                                                                                         caAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatcat                                           <  1:1817147/149‑1 (MQ=255)
                                                                                                             tCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAgg                                       <  1:1511743/149‑1 (MQ=255)
                                                                                                              cAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGc                                      <  1:2217947/149‑1 (MQ=255)
                                                                                                                    cGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCa                                >  1:2396546/1‑149 (MQ=255)
                                                                                                                      gAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGa                              >  2:4296088/1‑149 (MQ=255)
                                                                                                                       aaCGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGaa                             <  2:3371606/149‑1 (MQ=255)
                                                                                                                          ggTCACCGGCGACATGCGCAGGAAGAAATCGCCAAACTGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGc                          <  2:4983321/149‑1 (MQ=255)
                                                                                                                           gTCACCGGCGACATGCGCAGGAAGAAATCGCCAACATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGcc                         <  2:928513/149‑1 (MQ=255)
                                                                                                                             cACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCAc                       <  2:2719512/149‑1 (MQ=255)
                                                                                                                               ccGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACgg                     >  2:4223381/1‑149 (MQ=255)
                                                                                                                               ccGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACgg                     >  2:1628474/1‑149 (MQ=255)
                                                                                                                               ccGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACgg                     <  1:3206799/149‑1 (MQ=255)
                                                                                                                                cGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGa                    >  1:1409114/1‑149 (MQ=255)
                                                                                                                                 ggCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGaa                   >  1:2354068/1‑149 (MQ=255)
                                                                                                                                  gCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAAc                  <  2:2101173/149‑1 (MQ=255)
                                                                                                                                       aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc             >  2:3113376/1‑149 (MQ=255)
                                                                                                                                       aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc             <  1:3265742/149‑1 (MQ=255)
                                                                                                                                       aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc             >  2:1156014/1‑149 (MQ=255)
                                                                                                                                       aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc             >  1:4442334/1‑149 (MQ=255)
                                                                                                                                       aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc             <  1:657965/149‑1 (MQ=255)
                                                                                                                                              ggAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAcc      >  1:3354211/1‑149 (MQ=255)
                                                                                                                                              ggAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAcc      >  1:846668/1‑149 (MQ=255)
                                                                                                                                               gaagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCa     <  2:3784572/149‑1 (MQ=255)
                                                                                                                                                aagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCAc    <  1:5080176/149‑1 (MQ=255)
                                                                                                                                                aagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCAc    <  1:1297648/149‑1 (MQ=255)
                                                                                                                                                 agaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACt   <  1:5034126/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTT  >  CP009273/1229314‑1229608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑