BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,308,862	A→C	5.6%	S196R (AGC→CGC)	ompW →	outer membrane protein W

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,308,862	0	A	C	5.6%	846.8 / 4.8	293	S196R (AGC→CGC)	ompW	outer membrane protein W
Reads supporting (aligned to +/- strand): ref base A (162/104); new base C (2/23); total (164/129)
Fisher's exact test for biased strand distribution p-value = 1.81e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

TCTCAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCG  >  CP009273/1308714‑1309010

tctcAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATa                                                                                                                                                      >  1:3573305/1‑149 (MQ=255)

 ctcAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAg                                                                                                                                                     <  1:2907947/149‑1 (MQ=255)

  tcAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGc                                                                                                                                                    >  2:2875949/1‑149 (MQ=255)

     gTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTa                                                                                                                                                 <  2:1859940/149‑1 (MQ=255)

     gTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTa                                                                                                                                                 <  1:4470111/149‑1 (MQ=255)

     gTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTa                                                                                                                                                 <  2:4917733/149‑1 (MQ=255)

         gAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGcc                                                                                                                                             <  1:4208242/149‑1 (MQ=255)

         gAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGcc                                                                                                                                             <  1:2452679/149‑1 (MQ=255)

          aaaGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCt                                                                                                                                            >  1:1781530/1‑149 (MQ=255)

           aaGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTc                                                                                                                                           <  1:1240861/149‑1 (MQ=255)

           aaGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTc                                                                                                                                           <  1:264289/149‑1 (MQ=255)

              aTTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGAt                                                                                                                                        >  2:4604157/1‑149 (MQ=255)

               ttCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATc                                                                                                                                       >  1:3665466/1‑149 (MQ=255)

               ttCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATc                                                                                                                                       >  1:1071169/1‑149 (MQ=255)

               ttCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATc                                                                                                                                       <  2:92723/149‑1 (MQ=255)

                   tGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTg                                                                                                                                   >  2:375493/1‑149 (MQ=255)

                    ggggAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTgg                                                                                                                                  >  2:3087646/1‑149 (MQ=255)

                    ggggAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTgg                                                                                                                                  <  2:3693064/149‑1 (MQ=255)

                          cTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGttt                                                                                                                            <  2:154305/149‑1 (MQ=255)

                           tGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGAGACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTa                                                                                                                           <  2:3172699/149‑1 (MQ=255)

                            gCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTAt                                                                                                                          <  2:515210/149‑1 (MQ=255)

                               gggCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGtt                                                                                                                       >  2:198414/1‑149 (MQ=255)

                                  cAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTtctc                                                                                                                    <  1:5190173/149‑1 (MQ=255)

                                   aGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTca                                                                                                                   <  1:3404150/149‑1 (MQ=255)

                                    ggTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATCGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTcag                                                                                                                  >  1:3972127/1‑149 (MQ=255)

                                      tGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTcagca                                                                                                                <  1:2210949/149‑1 (MQ=255)

                                       gggggTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTcagcag                                                                                                               >  1:1004456/1‑149 (MQ=255)

                                       gggggTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTcagcag                                                                                                               >  1:158770/1‑149 (MQ=255)

                                           gTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGGGGGTGTTTATGTTCTCAGCAGGata                                                                                                           >  2:1686098/1‑149 (MQ=255)

                                            ttGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGatat                                                                                                          >  1:4344180/1‑149 (MQ=255)

                                               aTTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGt                                                                                                       >  1:4999992/1‑149 (MQ=255)

                                                     tGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTaa                                                                                                 >  1:3881069/1‑149 (MQ=255)

                                                      gATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAAt                                                                                                <  2:2258211/149‑1 (MQ=255)

                                                      gATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAAt                                                                                                >  1:1837247/1‑149 (MQ=255)

                                                        ttAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTc                                                                                              >  1:3183103/1‑149 (MQ=255)

                                                        ttAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTc                                                                                              >  1:1665823/1‑149 (MQ=255)

                                                          aaCCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCg                                                                                            >  2:1916637/1‑149 (MQ=255)

                                                           aCCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGAGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGc                                                                                           >  1:5126399/1‑149 (MQ=255)

                                                             cGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGcac                                                                                         <  2:2598599/149‑1 (MQ=255)

                                                             cGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGcac                                                                                         >  2:327774/1‑149 (MQ=255)

                                                              gTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGcaca                                                                                        >  1:2573560/1‑149 (MQ=255)

                                                                gACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACaaa                                                                                      >  1:27614/1‑149 (MQ=255)

                                                                   tggttggtTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAAc                                                                                   <  2:3427405/149‑1 (MQ=255)

                                                                     gttggttAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGa                                                                                 >  1:583202/1‑149 (MQ=255)

                                                                      ttggttAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGAc                                                                                >  1:4589717/1‑149 (MQ=255)

                                                                         gttAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGAcccc                                                                             >  1:3084131/1‑149 (MQ=255)

                                                                           tAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCTATCCGTGGGTGTTTATGTTCTCAGCTGTATTTCGTTTTTAATTCCGCACAAACACGACCCCGt                                                                           <  2:840300/149‑1 (MQ=255)

                                                                               aTGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAata                                                                       >  2:1728572/1‑149 (MQ=255)

                                                                               aTGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAata                                                                       >  1:2249224/1‑149 (MQ=255)

                                                                                 gTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAAtata                                                                     >  2:4361707/1‑149 (MQ=255)

                                                                                 gTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAAtata                                                                     >  2:3668818/1‑149 (MQ=255)

                                                                                  tCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATAc                                                                    >  2:1122729/1‑149 (MQ=255)

                                                                                  tCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATAc                                                                    >  2:2164998/1‑149 (MQ=255)

                                                                                    tgtgtGGTACATGTATATCTATACCCCCGCCTATTATAAGGTGGGGGGTGCACCGCAACACGATTGCGTCCGCCTCGATCGGTGGGTTTTTTTTTTCTCAGCTGTATATGGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgg                                                                  <  2:3524598/148‑1 (MQ=255)

                                                                                          ttACATGTATATCGATACCACCGCCAATTATAAGGTGGGCGGTGCACAGCAACCCGATAGCGTACGCCTCTATCGGTGGGTTTTTTGGTTCTCAGCAGGATATCTTTTTTAATTCCGCAAAAACACGACCCCGTAATATACGGGGTCaa                                                            <  2:445411/148‑1 (MQ=255)

                                                                                          gTACTTGGATATCTACACCACCGCCAATTATACGCTGGGCGGTGCCCACCAACCCTATCGCGTACGCCTCGATCCGTGGTTTTTTATGTTCTCAGAAGGATATCTTTTTTAATTCCGAACAAAAACGACCCCGTAATATACGGGGTCaa                                                            <  1:4718571/149‑1 (MQ=255)

                                                                                          gTAAATGTATATCGATACCACCGCCAATTATAAGCTGGGGGGTGCACAGCAACACGATAGCGTACGCCTCTAACCGGGGGTGTTTTTGTTCTCAGCAGTTATTGGTTTTTAATACCGCACAAACACGACCCCGTAATATACGGGGTCaa                                                            <  2:445519/149‑1 (MQ=255)

                                                                                           tACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAAt                                                           >  2:2979391/1‑149 (MQ=255)

                                                                                           tACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAAt                                                           >  1:3190286/1‑149 (MQ=255)

                                                                                            aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATa                                                          >  2:3781480/1‑149 (MQ=255)

                                                                                               tGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                       >  2:423630/1‑149 (MQ=255)

                                                                                               tGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                       >  1:3114928/1‑149 (MQ=255)

                                                                                                ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGa                                                      >  2:455493/1‑149 (MQ=255)

                                                                                                    atCGATCCCACCGCAAATTATAAGGTGGGCGGTGCACAGCAACACGATCGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCTGTATATGTTTTTTATTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                  <  1:2992070/149‑1 (MQ=255)

                                                                                                    atCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                  >  1:1026103/1‑149 (MQ=255)

                                                                                                    atCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                  >  1:3622547/1‑149 (MQ=255)

                                                                                                    atCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                  >  2:4085035/1‑149 (MQ=255)

                                                                                                     tCGATACCACCGCCAATTATAAGCTGGGGGGGGCACAGCAACCCGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCCGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                 <  2:3579028/149‑1 (MQ=255)

                                                                                                     tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                 >  1:686182/1‑149 (MQ=255)

                                                                                                      ctaTACCACCGCAATTTATACGCGGGGGGGGGCACAGCACCACGATCGGGTACGCCTCGTTCGGTGGGTGTTTTTGTTCTCAGCTGTTTCTCGTTTTTAATCCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                <  1:3310379/147‑1 (MQ=255)

                                                                                                      cGATACCACCGCAAATTATACGCGGGGCGGTGCACAGCAACCCGCTCGCGTCCGCCGCGATCCGGGGGTGTTTTTGTTCCCAGCAGGATAGCGTTTTTAATTCCGCAAAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                <  2:1386123/149‑1 (MQ=255)

                                                                                                      cGATACCACCGCAAATTATAAGCTGGGCGGTGCACAGAAACACGATAGCGTACGCCTCTAACCTTGGGTTTTTTTGTTCTCAGCAGTATATCTTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                <  1:3468375/149‑1 (MQ=255)

                                                                                                       taTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACTATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                               <  2:338678/148‑1 (MQ=255)

                                                                                                       gATACCCCCGCCAATTATAAGCTGGGCGGTGCACACCAACACGATAGCGTACGCCTCTATCGGTGGGTTTTTATTTTCTCAGCAGTATATCGTTTTTATTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                               <  2:4973602/149‑1 (MQ=255)

                                                                                                       gATACCACCGCCATTTTTATGCGGGGCGGTGCCCAGAAACACGATAGCGTACGCCTCGATCGGTGGGTGTTTTTGTTCTCAGATGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                               <  2:2440345/149‑1 (MQ=255)

                                                                                                       gATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                               >  2:3645862/1‑149 (MQ=255)

                                                                                                        aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtat                                              >  1:1360524/1‑149 (MQ=255)

                                                                                                         tACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtata                                             >  1:2497545/1‑149 (MQ=255)

                                                                                                           ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                           >  1:3631155/1‑149 (MQ=255)

                                                                                                                 ccATTTTTAAGCTGGGCGGTGCACAGCAACCCGATAGCGTACGCCTCGTTCCGTGGTTGTTTATGTTCTCAGCAGTATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                     <  2:2394274/149‑1 (MQ=255)

                                                                                                                  cAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatt                                    >  1:903702/1‑149 (MQ=255)

                                                                                                                  cAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGATTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatt                                    <  2:2102944/149‑1 (MQ=255)

                                                                                                                   aaTTATACGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGAGCCGTGGGTGTTTATGTTCTCAGCAGGATATCTTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                   <  1:3862342/149‑1 (MQ=255)

                                                                                                                    aTTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtattat                                  >  2:4722672/1‑149 (MQ=255)

                                                                                                                      tataAGCTGGGCGGTGCACAGCAACACCATCGCTGACGCCGCGAACCGTGGGTTTTTATGTTCCCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATCTACGGGGTCAATAAGGACATGGTATAAAGGGTATTAttt                                <  2:689904/149‑1 (MQ=255)

                                                                                                                         aaGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttctt                             >  1:73858/1‑149 (MQ=255)

                                                                                                                           gCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCg                           >  1:4832850/1‑149 (MQ=255)

                                                                                                                             tGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCt                         >  2:1419245/1‑149 (MQ=255)

                                                                                                                              gggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCtt                        >  2:770043/1‑149 (MQ=255)

                                                                                                                                ggggTGCACAGCAAAACGATAGCTTACGCCTCTAGCCTTGGGTGTTTATTTTCACAGCAGTATATCTTTTTTAATTCGGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCttat                      <  2:3961179/147‑3 (MQ=255)

                                                                                                                                ggggTACACACCAACACCAGCGCGTCCCCCTCGATCCGTGGTTTTTTTTGTTCTCAGCATTATATTTTTTTAAATTCCCAAAAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCt                      <  1:4392514/147‑1 (MQ=255)

                                                                                                                                 gggTGCACCGAAACACTATCGCGTACGCCTCGTTCGGTGGTTTTTTTTTTTATCAGCTGTATATGTTTTTTAATACCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTa                     <  1:1523557/148‑1 (MQ=255)

                                                                                                                                     gCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGcc                 >  1:2106846/1‑149 (MQ=255)

                                                                                                                                          gCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAg            >  2:5061378/1‑149 (MQ=255)

                                                                                                                                          gCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAg            >  2:3796818/1‑149 (MQ=255)

                                                                                                                                              cacTATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc        <  2:5175760/149‑1 (MQ=255)

                                                                                                                                              cacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc        <  2:2116519/149‑1 (MQ=255)

                                                                                                                                               ccGATAGCGTCCGCCTCGTTCGGTGGGTGTTTTTGTTCTCAGCAGTTTTTGTTTTTTAATTCCGCAAAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa       <  1:4639136/148‑1 (MQ=255)

                                                                                                                                               acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa       >  2:964233/1‑149 (MQ=255)

                                                                                                                                                  aTAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCGGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag    <  2:5161271/149‑1 (MQ=255)

TCTCAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTTGGTTAACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCG  >  CP009273/1308714‑1309010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`