Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 127,629 G→T 5.3% P40Q (CCG→CAG)  yacH ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273127,6290GT 5.3% 1361.1 / 35.9 347P40Q (CCG→CAG) yacHuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (145/183);  new base T (9/10);  total (154/193)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

GGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGT  >  CP009273/127482‑127776
                                                                                                                                                   |                                                                                                                                                   
ggATACAGCGCGACGGGCGCAACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCgg                                                                                                                                                    <  1:2050013/149‑1 (MQ=255)
 gATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGt                                                                                                                                                   >  1:2838202/1‑149 (MQ=255)
  aTACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCTGtt                                                                                                                                                  <  2:4136076/149‑1 (MQ=255)
  aTACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGtt                                                                                                                                                  >  2:2076554/1‑149 (MQ=255)
    aCAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTcc                                                                                                                                                <  1:4647350/149‑1 (MQ=255)
     cAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCca                                                                                                                                               <  2:5085778/149‑1 (MQ=255)
       gcgcGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCcaca                                                                                                                                             >  1:3761738/1‑149 (MQ=255)
       gcgcGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCcaca                                                                                                                                             >  1:3661245/1‑149 (MQ=255)
       gcgcGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCcaca                                                                                                                                             <  2:3825881/149‑1 (MQ=255)
         gcgACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCGGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAgg                                                                                                                                           >  2:1443947/1‑149 (MQ=255)
                gcgcGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTcc                                                                                                                                    >  2:837751/1‑149 (MQ=255)
                  gcgACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCTGTTCCACAGGCGCTTCCAc                                                                                                                                  <  1:872753/149‑1 (MQ=255)
                  gcgACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCAc                                                                                                                                  <  1:442900/149‑1 (MQ=255)
                     aCCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACtgt                                                                                                                               >  1:1176865/1‑149 (MQ=255)
                     aCCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACtgt                                                                                                                               <  2:4484961/149‑1 (MQ=255)
                     aCCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACtgt                                                                                                                               <  1:1534945/149‑1 (MQ=255)
                     aCCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGAGCTTCCACtgt                                                                                                                               >  2:1100738/1‑149 (MQ=255)
                       ccATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGc                                                                                                                             <  2:4189150/149‑1 (MQ=255)
                        cATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCg                                                                                                                            >  2:4263096/1‑149 (MQ=255)
                        cATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCg                                                                                                                            >  2:2441560/1‑149 (MQ=255)
                         aTTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCgg                                                                                                                           >  1:1500627/1‑149 (MQ=255)
                          ttGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGc                                                                                                                          >  1:4339204/1‑149 (MQ=255)
                           tGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCt                                                                                                                         <  2:4528165/149‑1 (MQ=255)
                             aTCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCTGTTCCACAGGCGCTTCCACTGTGCGGCttt                                                                                                                       >  1:359423/1‑149 (MQ=255)
                             aTCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCttt                                                                                                                       >  1:2765142/1‑149 (MQ=255)
                             aTCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCttt                                                                                                                       >  2:3232090/1‑149 (MQ=255)
                             aTCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCttt                                                                                                                       >  2:2368801/1‑149 (MQ=255)
                              tCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCtttt                                                                                                                      <  1:2899137/149‑1 (MQ=255)
                                tATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTta                                                                                                                    >  2:1924646/1‑149 (MQ=255)
                                tATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTta                                                                                                                    >  2:1777277/1‑149 (MQ=255)
                                 aTTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTtat                                                                                                                   >  1:4036824/1‑149 (MQ=255)
                                  tttGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTtata                                                                                                                  >  2:2556071/1‑149 (MQ=255)
                                          gTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGccc                                                                                                          >  1:3149705/1‑149 (MQ=255)
                                           tGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCg                                                                                                         <  1:1435634/149‑1 (MQ=255)
                                             cTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGt                                                                                                       >  1:3418337/1‑149 (MQ=255)
                                                 aTGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTagag                                                                                                   <  1:1203469/149‑1 (MQ=255)
                                                  tGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAgg                                                                                                  <  2:4855394/149‑1 (MQ=255)
                                                   gCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACAGTGCGGCTTTTTAAATATATCCCCGTAGAggc                                                                                                 >  1:2666810/1‑149 (MQ=255)
                                                    cGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAggcg                                                                                                <  1:3830238/149‑1 (MQ=255)
                                                      gATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAggcggc                                                                                              >  1:207912/1‑149 (MQ=255)
                                                             cgacgGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTACACTGTGCGGCTTTTTATATATATCCCGGTAGAGGCGGCACaaatc                                                                                       >  1:3201364/1‑147 (MQ=255)
                                                              gacgGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGcc                                                                                      <  1:2818059/149‑1 (MQ=255)
                                                               acgGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGccc                                                                                     >  2:1679641/1‑149 (MQ=255)
                                                               acgGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGccc                                                                                     <  2:956328/149‑1 (MQ=255)
                                                                    gggTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCAc                                                                                <  1:638871/149‑1 (MQ=255)
                                                                          tACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCaaa                                                                          <  1:2299366/149‑1 (MQ=255)
                                                                          tACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCaaa                                                                          <  1:4962825/149‑1 (MQ=255)
                                                                           aCGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAAt                                                                         >  1:4676491/1‑149 (MQ=255)
                                                                             gggAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTa                                                                       >  2:3092567/1‑149 (MQ=255)
                                                                             gggAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTa                                                                       <  2:1519116/149‑1 (MQ=255)
                                                                             gggAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTa                                                                       <  2:2118063/149‑1 (MQ=255)
                                                                               gAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAAATAgc                                                                     >  1:4103508/1‑149 (MQ=255)
                                                                                    gCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAg                                                                <  2:1333834/149‑1 (MQ=255)
                                                                                     cGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGc                                                               >  2:1216156/1‑149 (MQ=255)
                                                                                       gAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGcac                                                             >  2:666827/1‑149 (MQ=255)
                                                                                          aCCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATg                                                          <  2:2988366/149‑1 (MQ=255)
                                                                                           ccGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATgg                                                         <  2:3298356/149‑1 (MQ=255)
                                                                                           ccGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATgg                                                         >  2:4922852/1‑149 (MQ=255)
                                                                                            cGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCCCATggg                                                        >  1:3424640/1‑149 (MQ=255)
                                                                                              ttGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGt                                                      <  2:2861258/149‑1 (MQ=255)
                                                                                               tGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGtt                                                     <  2:4943665/149‑1 (MQ=255)
                                                                                                gCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGttt                                                    >  2:2048457/1‑149 (MQ=255)
                                                                                                gCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGttt                                                    >  2:4238570/1‑149 (MQ=255)
                                                                                                gCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGttt                                                    >  2:626064/1‑149 (MQ=255)
                                                                                                gCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGttt                                                    >  2:1123598/1‑149 (MQ=255)
                                                                                                    gAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCTGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATAGAGCCCGGTCGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAAc                                                <  1:60925/149‑1 (MQ=255)
                                                                                                    gAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAAc                                                <  2:835348/149‑1 (MQ=255)
                                                                                                     aaaCGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACg                                               <  1:4169116/149‑1 (MQ=255)
                                                                                                          tAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCaaa                                          <  2:3974770/149‑1 (MQ=255)
                                                                                                           aaCCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACCTGGGGTTTAAACGGCAAAg                                         >  1:4595752/1‑149 (MQ=255)
                                                                                                           aaCCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAg                                         <  2:2588598/149‑1 (MQ=255)
                                                                                                            aCCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGACTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGt                                        <  1:2422995/149‑1 (MQ=255)
                                                                                                              cgcgGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCa                                      <  1:2586460/149‑1 (MQ=255)
                                                                                                               gcgGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAt                                     <  1:1027452/149‑1 (MQ=255)
                                                                                                                 ggCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAttt                                   <  2:4847130/149‑1 (MQ=255)
                                                                                                                 ggCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAttt                                   <  1:3322162/149‑1 (MQ=255)
                                                                                                                  gCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAtttt                                  <  1:4894605/149‑1 (MQ=255)
                                                                                                                    aGCGTCAGACACAGCCAGTTGTGTCGATTGCTGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCa                                >  2:4651440/1‑149 (MQ=255)
                                                                                                                    aGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCCAAGTCATTTTCa                                >  2:4612355/1‑149 (MQ=255)
                                                                                                                    aGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCa                                <  1:5186090/149‑1 (MQ=255)
                                                                                                                       gTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATaa                             <  1:1777277/149‑1 (MQ=255)
                                                                                                                       gTCAGACACAGCCAGTTGTGTCGATTGAGGTTCCAGAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATaa                             <  2:2521134/149‑1 (MQ=255)
                                                                                                                        tCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAAt                            <  2:2688707/149‑1 (MQ=255)
                                                                                                                        tCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAAt                            <  1:2936280/149‑1 (MQ=255)
                                                                                                                        tCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAAt                            <  2:537341/149‑1 (MQ=255)
                                                                                                                         cAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAAtt                           <  1:1896576/149‑1 (MQ=255)
                                                                                                                          aGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTc                          >  2:393991/1‑149 (MQ=255)
                                                                                                                             cacaGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGa                       <  1:4211506/149‑1 (MQ=255)
                                                                                                                              acaGCCAGTTGTGTCGATTGCTGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGAt                      <  2:871345/149‑1 (MQ=255)
                                                                                                                                   cAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAATTTCATAATTCGGATCTCaa                 >  2:1957370/1‑149 (MQ=255)
                                                                                                                                    aGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAg                >  2:3732448/1‑149 (MQ=255)
                                                                                                                                        gtgtCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGaaa            <  2:3654226/149‑1 (MQ=255)
                                                                                                                                        gtgtCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGaaa            <  1:1664443/149‑1 (MQ=255)
                                                                                                                                         tgtCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAAt           <  2:1816377/149‑1 (MQ=255)
                                                                                                                                         tgtCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATAAAGCCCGGTAGAGGCGGCACAAAGCCCGGCACAGCAAAATAGCGCCAGCACAATGGGTTTAAACGGCAAAGTCATTTTCATAATTATGATATCAAGGaaaa           >  2:4387487/1‑148 (MQ=255)
                                                                                                                                          gtCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATc          <  2:1196267/149‑1 (MQ=255)
                                                                                                                                          gtCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATc          <  1:3034063/149‑1 (MQ=255)
                                                                                                                                                tGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATg    <  1:2221352/149‑1 (MQ=255)
                                                                                                                                                 gCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATgg   <  2:1659053/149‑1 (MQ=255)
                                                                                                                                                 gCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATgg   <  1:4534222/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGATACAGCGCGACGGGCGCGACCCATTGATCTATTTGTGCAGTGCTGAATGCGGATTTGACGACGGCGGGTGTTACGGGAGGTGCGGAAACCGTTGCAGGAAACGTAACCGCGGCAGCGTCAGACACAGCCAGTTGTGTCGATTGCGGTTCCACAGGCGCTTCCACTGTGCGGCTTTTTATATATAGCCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGT  >  CP009273/127482‑127776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑