BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,342,794	C→T	5.9%	D126N (GAC→AAC)	rnb ←	ribonuclease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,342,794	0	C	T	5.9%	1101.6 / 36.9	303	D126N (GAC→AAC)	rnb	ribonuclease II
Reads supporting (aligned to +/- strand): ref base C (145/139); new base T (9/9); total (154/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

GGTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCT  >  CP009273/1342646‑1342942

ggTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTc                                                                                                                                                      >  1:3607409/1‑149 (MQ=255)

ggTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTc                                                                                                                                                      >  2:436303/1‑149 (MQ=255)

  tGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGc                                                                                                                                                    <  1:4661810/149‑1 (MQ=255)

   gCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGcc                                                                                                                                                   >  1:2584555/1‑149 (MQ=255)

     ttcttTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCctt                                                                                                                                                 >  1:2379300/1‑149 (MQ=255)

        tttttCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCcttctt                                                                                                                                              <  1:3161006/149‑1 (MQ=255)

          tttCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTa                                                                                                                                            <  2:703925/149‑1 (MQ=255)

              cAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACt                                                                                                                                        <  2:2457326/149‑1 (MQ=255)

               aGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTc                                                                                                                                       >  2:1518039/1‑149 (MQ=255)

                 aTTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGt                                                                                                                                     <  1:1508028/149‑1 (MQ=255)

                 aTTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGt                                                                                                                                     <  1:4103065/149‑1 (MQ=255)

                    aTGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGt                                                                                                                                  >  2:3099222/1‑149 (MQ=255)

                     tGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTAGTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGACGCCTTCTTTAAACTCGTGGtt                                                                                                                                 >  2:4304607/1‑149 (MQ=255)

                          tGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGc                                                                                                                            >  1:1996980/1‑149 (MQ=255)

                            cAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCa                                                                                                                          >  2:4056133/1‑149 (MQ=255)

                              aGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACg                                                                                                                        <  1:3075438/149‑1 (MQ=255)

                                    aCCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTgc                                                                                                                  <  1:964224/149‑1 (MQ=255)

                                      ccaccaCGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTgcgc                                                                                                                <  2:2892134/149‑1 (MQ=255)

                                      ccaccaCGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTgcgc                                                                                                                >  2:176856/1‑149 (MQ=255)

                                        accacGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCgg                                                                                                              >  2:1617178/1‑149 (MQ=255)

                                             ggTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAgg                                                                                                         <  1:1337621/149‑1 (MQ=255)

                                              gTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGa                                                                                                        <  1:1490361/149‑1 (MQ=255)

                                               tACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGaa                                                                                                       >  1:5105829/1‑149 (MQ=255)

                                               tACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGaa                                                                                                       <  2:2384887/149‑1 (MQ=255)

                                                aCAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAAt                                                                                                      >  2:3174003/1‑149 (MQ=255)

                                                aCAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAAt                                                                                                      <  2:3453779/149‑1 (MQ=255)

                                                aCAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAAt                                                                                                      <  2:1679485/149‑1 (MQ=255)

                                                aCAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAAt                                                                                                      >  2:1305055/1‑149 (MQ=255)

                                                 cAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCTCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATg                                                                                                     <  1:75424/149‑1 (MQ=255)

                                                  aaaGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATgg                                                                                                    <  1:3176339/149‑1 (MQ=255)

                                                   aaGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGc                                                                                                   >  1:4232756/1‑149 (MQ=255)

                                                   aaGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGc                                                                                                   >  2:2255395/1‑149 (MQ=255)

                                                   aaGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGc                                                                                                   >  2:4858661/1‑149 (MQ=255)

                                                   aaGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGc                                                                                                   >  1:3211075/1‑149 (MQ=255)

                                                     gTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGt                                                                                                 <  2:1121466/149‑1 (MQ=255)

                                                     gTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGt                                                                                                 <  2:3383311/149‑1 (MQ=255)

                                                      tGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTc                                                                                                >  1:4402977/1‑149 (MQ=255)

                                                       gATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCt                                                                                               <  2:5082086/149‑1 (MQ=255)

                                                       gATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCt                                                                                               >  2:4589684/1‑149 (MQ=255)

                                                        aTCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCtt                                                                                              >  2:4882471/1‑149 (MQ=255)

                                                        aTCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCtt                                                                                              <  2:4706726/149‑1 (MQ=255)

                                                         tCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCttt                                                                                             >  1:2727763/1‑149 (MQ=255)

                                                         tCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCttt                                                                                             >  1:1551273/1‑149 (MQ=255)

                                                               ccAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAagag                                                                                       >  1:598772/1‑149 (MQ=255)

                                                               ccAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAagag                                                                                       <  2:3608927/149‑1 (MQ=255)

                                                                cAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGt                                                                                      <  1:5168815/149‑1 (MQ=255)

                                                                cAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGt                                                                                      >  2:1840791/1‑149 (MQ=255)

                                                                cAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGt                                                                                      <  1:3267222/149‑1 (MQ=255)

                                                                 aaaaGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTg                                                                                     <  2:4326450/149‑1 (MQ=255)

                                                                 aaaaGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTg                                                                                     >  2:2283204/1‑149 (MQ=255)

                                                                  aaaGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTgg                                                                                    >  2:3552854/1‑149 (MQ=255)

                                                                   aaGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGga                                                                                   >  1:4761200/1‑149 (MQ=255)

                                                                   aaGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGga                                                                                   <  1:1472364/149‑1 (MQ=255)

                                                                     gTGATGTATTGTGTCAGTTCTGCATAGAAAGAACTATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGgatg                                                                                 >  1:1425008/1‑149 (MQ=255)

                                                                     gTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGgatg                                                                                 >  1:3768942/1‑149 (MQ=255)

                                                                      tGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGgatga                                                                                <  1:4926092/149‑1 (MQ=255)

                                                                      tGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGgatga                                                                                <  1:1597288/149‑1 (MQ=255)

                                                                        aTGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATc                                                                              >  1:2244345/1‑149 (MQ=255)

                                                                        aTGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATc                                                                              >  2:2077689/1‑149 (MQ=255)

                                                                        aTGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATc                                                                              >  2:4506972/1‑149 (MQ=255)

                                                                            aTTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGa                                                                          <  2:1421470/149‑1 (MQ=255)

                                                                             ttGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGaa                                                                         >  1:3443850/1‑149 (MQ=255)

                                                                               gtgtCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACg                                                                       >  1:444793/1‑149 (MQ=255)

                                                                               gtgtCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCAGCGCGGCAAGGAATGGCGTCTGTTAAGAGTGGAGGATCAGGAACg                                                                       >  1:3955534/1‑149 (MQ=255)

                                                                                tgtCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGa                                                                      <  1:2665420/149‑1 (MQ=255)

                                                                                     gTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGcc                                                                 >  1:1125616/1‑149 (MQ=255)

                                                                                      ttCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCa                                                                >  1:1194492/1‑149 (MQ=255)

                                                                                      ttCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCa                                                                <  2:1070592/149‑1 (MQ=255)

                                                                                      ttCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGCGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCa                                                                <  1:3045123/149‑1 (MQ=255)

                                                                                        cTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGa                                                              <  2:4249568/149‑1 (MQ=255)

                                                                                        cTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGa                                                              <  1:3900853/149‑1 (MQ=255)

                                                                                        cTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGa                                                              <  2:302532/149‑1 (MQ=255)

                                                                                          gCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACg                                                            <  2:2540250/149‑1 (MQ=255)

                                                                                                aaagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAtt                                                      >  2:2010367/1‑149 (MQ=255)

                                                                                                 aagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAttt                                                     >  1:4897103/1‑149 (MQ=255)

                                                                                                 aagaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAttt                                                     <  1:2821425/149‑1 (MQ=255)

                                                                                                  agaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAtttt                                                    <  1:1013274/149‑1 (MQ=255)

                                                                                                  agaaCGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCAtttt                                                    >  2:527085/1‑149 (MQ=255)

                                                                                                       gATCGCCTTTCTGAGGATGACGGCTCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCTCGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGccc                                               <  1:1587939/149‑1 (MQ=255)

                                                                                                       gATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGccc                                               >  1:3532862/1‑149 (MQ=255)

                                                                                                       gATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGccc                                               >  1:2078432/1‑149 (MQ=255)

                                                                                                        aTCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCt                                              >  2:3872855/1‑149 (MQ=255)

                                                                                                          cGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGACCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGa                                            >  2:606186/1‑149 (MQ=255)

                                                                                                              tttCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGTAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCt                                        <  2:1183199/149‑1 (MQ=255)

                                                                                                              tttCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCt                                        <  2:2945556/149‑1 (MQ=255)

                                                                                                                  aGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTAcc                                    >  2:2813621/1‑149 (MQ=255)

                                                                                                                   gCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTAccc                                   <  1:1696783/149‑1 (MQ=255)

                                                                                                                        tGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGaa                              <  1:2618722/149‑1 (MQ=255)

                                                                                                                         gACGGCGCATTTCGGCAACCGCCCAGTTGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGaaa                             <  2:844736/149‑1 (MQ=255)

                                                                                                                         gACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGaaa                             >  1:1873921/1‑149 (MQ=255)

                                                                                                                             gcgcATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAg                         <  2:801742/149‑1 (MQ=255)

                                                                                                                                 aTTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAg                     <  1:1798730/149‑1 (MQ=255)

                                                                                                                                  tttCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAgg                    >  2:1734060/1‑149 (MQ=255)

                                                                                                                                         aaCCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGtt             <  2:1923365/149‑1 (MQ=255)

                                                                                                                                         aaCCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGtt             >  1:3831725/1‑149 (MQ=255)

                                                                                                                                          aCCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTc            >  1:3662557/1‑149 (MQ=255)

                                                                                                                                             gCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAAc         <  1:787158/149‑1 (MQ=255)

                                                                                                                                             gCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAAc         <  2:2735961/149‑1 (MQ=255)

                                                                                                                                               ccAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCa       <  2:5154357/149‑1 (MQ=255)

                                                                                                                                                   tCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGttc   <  2:419275/149‑1 (MQ=255)

GGTGCTTCTTTTTCCAGATTATGGCGTGCAAGGGTAACCCACCACGGTACAAAGTGATCGTCACCAAAAGTGATGTATTGTGTCAGTTCTGCATAGAAAGAACGATCGCCTTTCAGCGGATGACGGCGCATTTCGGCAACCGCCCAGTCGCCTTCTTTAAACTCGTGGTTCAGGCCACGGGCTGCGCGGCAAGGAATGGCGTCTTTTAAGAGTGGATGATCAGGAACGATGGCCAGACGGTCATTTTTGCCCTGAACCTTACCCACGAAACGAGTCAGGAACGGTTCAACCAGTTCT  >  CP009273/1342646‑1342942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`