BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,521,211	A→T	6.9%	L5F (TTA→TTT)	yncH →	IPR020099 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,521,211	0	A	T	6.9%	944.8 / 47.5	259	L5F (TTA→TTT)	yncH	IPR020099 family protein
Reads supporting (aligned to +/- strand): ref base A (108/133); new base T (8/10); total (116/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 271 total aligned reads displayed.

ATGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTG  >  CP009273/1521063‑1521359

aTGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTa                                                                                                                                                      >  1:1144870/1‑149 (MQ=255)

 tGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTaa                                                                                                                                                     >  2:3960204/1‑149 (MQ=255)

 tGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTaa                                                                                                                                                     >  1:597928/1‑149 (MQ=255)

 tGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTaa                                                                                                                                                     >  1:4529399/1‑149 (MQ=255)

 tGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTaa                                                                                                                                                     <  2:706527/149‑1 (MQ=255)

   cTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAAtt                                                                                                                                                   <  2:1201391/149‑1 (MQ=255)

   cTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAAtt                                                                                                                                                   <  1:2710940/149‑1 (MQ=255)

    tGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAAttt                                                                                                                                                  >  2:2447834/1‑149 (MQ=255)

    tGTATGTCAGTTACAGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCTGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAAttt                                                                                                                                                  <  2:517184/149‑1 (MQ=255)

     gTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTta                                                                                                                                                 >  2:2542883/1‑149 (MQ=255)

       aTGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTtata                                                                                                                                               <  1:4117760/149‑1 (MQ=255)

        tGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTTATTTatat                                                                                                                                              <  2:3054074/149‑1 (MQ=255)

        tGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTTATTTatat                                                                                                                                              >  1:3735783/1‑149 (MQ=255)

        tGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTatat                                                                                                                                              >  1:1850211/1‑149 (MQ=255)

         gTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCAACAATGATGGGCGTAATTAAATAAATTCATGGTATGTTTTTTAATTTatata                                                                                                                                             >  1:3692458/1‑148 (MQ=255)

          tCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCa                                                                                                                                            <  2:4450725/149‑1 (MQ=255)

          tCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCa                                                                                                                                            <  2:319906/149‑1 (MQ=255)

           cAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCAc                                                                                                                                           <  2:2851609/149‑1 (MQ=255)

             gTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCActc                                                                                                                                         <  2:1389373/149‑1 (MQ=255)

             gTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCActc                                                                                                                                         <  1:3804162/149‑1 (MQ=255)

               tACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAATTTCATGGTATGTTTTTTAATTTATATCACtctc                                                                                                                                       >  2:3782846/1‑149 (MQ=255)

                aCCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTTATTTATATCACTCTcc                                                                                                                                      <  2:1579518/149‑1 (MQ=255)

                 ccGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCt                                                                                                                                     >  2:550367/1‑149 (MQ=255)

                 ccGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCt                                                                                                                                     >  1:4210480/1‑149 (MQ=255)

                  cGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCtt                                                                                                                                    <  2:3660711/149‑1 (MQ=255)

                     aaTTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTttc                                                                                                                                 <  1:214671/149‑1 (MQ=255)

                      aTTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTttca                                                                                                                                <  2:4643380/149‑1 (MQ=255)

                      aTTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTttca                                                                                                                                >  2:4775309/1‑149 (MQ=255)

                        tACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTttcatt                                                                                                                              >  1:1842521/1‑149 (MQ=255)

                            agaagTATTAAAAAGGAATGAACTTATTTGAGTGGGATACCAGCAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATGAATTAAATTCATGGGATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGc                                                                                                                          <  2:2300830/149‑1 (MQ=255)

                            agaagTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGc                                                                                                                          <  2:4531959/149‑1 (MQ=255)

                            agaagTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGc                                                                                                                          >  1:2351649/1‑149 (MQ=255)

                             gaagTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTAAGCg                                                                                                                         >  1:3670071/1‑149 (MQ=255)

                              aagTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCgt                                                                                                                        <  2:78596/149‑1 (MQ=255)

                                  aTTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCt                                                                                                                    <  2:1108625/149‑1 (MQ=255)

                                   ttAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTa                                                                                                                   >  2:3336957/1‑149 (MQ=255)

                                          ggAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTatttcatt                                                                                                            <  2:2589214/149‑1 (MQ=255)

                                             aTGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTcc                                                                                                         >  2:4869367/1‑149 (MQ=255)

                                             aTGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTcc                                                                                                         >  2:2266018/1‑149 (MQ=255)

                                             aTGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTcc                                                                                                         >  1:3096992/1‑149 (MQ=255)

                                               gAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCctc                                                                                                       <  2:5103330/149‑1 (MQ=255)

                                               gAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCctc                                                                                                       <  2:4228006/149‑1 (MQ=255)

                                                aaaTTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCctct                                                                                                      <  1:1917030/149‑1 (MQ=255)

                                                    tattTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTTATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGaaa                                                                                                  >  1:3396823/1‑149 (MQ=255)

                                                     attTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGaaaa                                                                                                 >  1:428264/1‑149 (MQ=255)

                                                     attTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGaaaa                                                                                                 >  2:1994864/1‑149 (MQ=255)

                                                      tttGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGTAAAg                                                                                                >  1:4403143/1‑149 (MQ=255)

                                                      tttGAGTGGGATACCAGAAAATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAg                                                                                                >  1:3636637/1‑149 (MQ=255)

                                                         gAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGaaa                                                                                             <  1:2676548/149‑1 (MQ=255)

                                                             gggATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAaactaa                                                                                         >  2:3399727/1‑149 (MQ=255)

                                                              ggATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAaactaac                                                                                        >  2:1996764/1‑149 (MQ=255)

                                                                aTACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAactaacta                                                                                      <  2:2891297/149‑1 (MQ=255)

                                                                  aCCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTAtt                                                                                    <  1:1804736/149‑1 (MQ=255)

                                                                     agaagaTTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTcac                                                                                 >  2:4428661/1‑149 (MQ=255)

                                                                     agaagaTTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTcac                                                                                 <  2:886607/149‑1 (MQ=255)

                                                                     agaagaTTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTATGAATAGTAACTAACTATTcac                                                                                 >  2:406587/1‑149 (MQ=255)

                                                                      gaagaTTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTcaca                                                                                >  2:128435/1‑149 (MQ=255)

                                                                          aTTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGt                                                                            <  1:1042290/149‑1 (MQ=255)

                                                                           ttATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTg                                                                           <  1:3016842/149‑1 (MQ=255)

                                                                             aTGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCa                                                                         <  2:2341870/149‑1 (MQ=255)

                                                                                ttatttatTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGAt                                                                      <  2:1057097/149‑1 (MQ=255)

                                                                                   tttattATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGtt                                                                   >  1:2629183/1‑149 (MQ=255)

                                                                                    ttattaTTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGttt                                                                  <  1:1351713/149‑1 (MQ=255)

                                                                                    ttattaTTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTAACATCGTGCAGATGttt                                                                  >  2:2721733/1‑149 (MQ=255)

                                                                                       ttattCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAg                                                               >  1:662376/1‑149 (MQ=255)

                                                                                         atacAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTATTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAgtt                                                             <  2:4601536/146‑1 (MQ=255)

                                                                                             aGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTa                                                         <  2:5198820/149‑1 (MQ=255)

                                                                                               tAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATc                                                       >  2:3726258/1‑149 (MQ=255)

                                                                                                 aCTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATcac                                                     >  1:1305438/1‑149 (MQ=255)

                                                                                                   tttATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAg                                                   <  2:1699361/149‑1 (MQ=255)

                                                                                                   tttATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAg                                                   >  1:468772/1‑149 (MQ=255)

                                                                                                      aTCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCa                                                >  1:3245219/1‑149 (MQ=255)

                                                                                                       tCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCAt                                               <  1:3363954/149‑1 (MQ=255)

                                                                                                         cacaATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTc                                             >  1:5151635/1‑149 (MQ=255)

                                                                                                          acaATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCt                                            >  2:927538/1‑149 (MQ=255)

                                                                                                            aaTGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCtat                                          <  1:940656/149‑1 (MQ=255)

                                                                                                              tgatgGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATaa                                        <  1:4020990/149‑1 (MQ=255)

                                                                                                                atgGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAAtc                                      <  1:2335480/149‑1 (MQ=255)

                                                                                                                atgGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAAtc                                      <  2:2220396/149‑1 (MQ=255)

                                                                                                                  gggCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAAtctc                                    <  2:2284057/149‑1 (MQ=255)

                                                                                                                     cGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCaaa                                 <  1:2266018/149‑1 (MQ=255)

                                                                                                                     cGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCaaa                                 <  1:3203884/149‑1 (MQ=255)

                                                                                                                     cGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCaaa                                 <  1:4869367/149‑1 (MQ=255)

                                                                                                                         attaattaAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGt                             >  2:197189/1‑149 (MQ=255)

                                                                                                                           taattaaATTCATGGTATGTTTTTTTATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGttt                           <  2:3113789/149‑1 (MQ=255)

                                                                                                                            aattaaATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGtttt                          <  1:1995364/149‑1 (MQ=255)

                                                                                                                            aattaaATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGtttt                          <  1:1994864/149‑1 (MQ=255)

                                                                                                                             attaaATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGttttt                         >  1:4985499/1‑149 (MQ=255)

                                                                                                                              ttaaATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTa                        <  2:3251083/149‑1 (MQ=255)

                                                                                                                                aaaTTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAat                      >  2:2784393/1‑149 (MQ=255)

                                                                                                                                  aTTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAatga                    <  1:476428/149‑1 (MQ=255)

                                                                                                                                  aTTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAatga                    <  1:1131379/149‑1 (MQ=255)

                                                                                                                                     cATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATgg                 <  1:2529261/149‑1 (MQ=255)

                                                                                                                                     cATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATgg                 <  1:1666630/149‑1 (MQ=255)

                                                                                                                                      aTGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGa                >  2:2998346/1‑149 (MQ=255)

                                                                                                                                       tGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGat               <  1:4942719/149‑1 (MQ=255)

                                                                                                                                        ggTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGata              <  2:714218/149‑1 (MQ=255)

                                                                                                                                         gTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGatat             <  1:550367/149‑1 (MQ=255)

                                                                                                                                            tGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCt          <  1:4008914/149‑1 (MQ=255)

                                                                                                                                                    aaTTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTg  <  2:2340248/149‑1 (MQ=255)

ATGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTATCCACAATGATGGGCGTAATTAATTAAATTCATGGTATGTTTTTTAATTTATATCACTCTCCTTTTCATTCAGCGTGTCTATTTCATTTCCTCTGAAAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTG  >  CP009273/1521063‑1521359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`