BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,784,723	A→C	8.1%	F260C (TTT→TGT)	ydiU ←	UPF0061 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,784,723	0	A	C	8.1%	729.3 / 7.9	267	F260C (TTT→TGT)	ydiU	UPF0061 family protein
Reads supporting (aligned to +/- strand): ref base A (163/75); new base C (2/27); total (165/102)
Fisher's exact test for biased strand distribution p-value = 9.11e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

GGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATC  >  CP009273/1784575‑1784871

ggCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCaa                                                                                                                                                      <  1:686804/149‑1 (MQ=255)

   aTCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCACACg                                                                                                                                                   >  1:4152850/1‑149 (MQ=255)

     cTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGc                                                                                                                                                 >  2:3291478/1‑149 (MQ=255)

     cTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGc                                                                                                                                                 >  1:1707775/1‑149 (MQ=255)

      tACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGcc                                                                                                                                                >  1:1235447/1‑149 (MQ=255)

          gCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTa                                                                                                                                            >  2:2049027/1‑149 (MQ=255)

           caaaaaATGGCGACAATTTCTGAGCCAGACGCTGTAAATTCCACAACGCGACGGCATGTTTATTATCAAAGCTTTAACGCCCTTGATTATCCGAGTGATTACAAATAAAACCGGGTTCTTAATAATCAAGAAAACAAAACGGCCCGTaa                                                                                                                                           <  2:5166118/149‑1 (MQ=255)

            aacaaATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAAt                                                                                                                                          >  1:1681822/1‑149 (MQ=255)

             acaaATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATc                                                                                                                                         >  2:302904/1‑149 (MQ=255)

                aaTGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAg                                                                                                                                      >  2:3656843/1‑149 (MQ=255)

                aaTGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAg                                                                                                                                      >  2:133317/1‑149 (MQ=255)

                  tGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCg                                                                                                                                    <  2:3867113/149‑1 (MQ=255)

                    gCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTTATTATCAAAGCTGTAACGCCATTGATGATCCGAGTTATTACAAATAAAACCGGGTTCGTAATAATCAAGAAAACCAAACGGCCCGTAATCAAGCGTc                                                                                                                                  <  2:652020/149‑1 (MQ=255)

                    gCGACAAGGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTTTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATAATCAAGAAAACCAAACGGCCCGTAATCAAGCGTc                                                                                                                                  <  2:2920030/149‑1 (MQ=255)

                       aCAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGc                                                                                                                               >  1:3216903/1‑149 (MQ=255)

                             tCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCcagc                                                                                                                         >  2:510584/1‑149 (MQ=255)

                             tCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCcagc                                                                                                                         >  1:3316804/1‑149 (MQ=255)

                              cTGCGCCAGACGCTGTAAAGTCCACAACGCGACGGCTGGTTTATTATCAACGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAAACAAAAAGAAACCCAAACGGCCCGTAATCAAGCGTCAGCCCcagca                                                                                                                        <  2:1884340/149‑1 (MQ=255)

                               tGCGCCAGACGTTTTAATTCCCACACCGCGACGGCTGGTTTTTTATCAACGCTTTAACGCCCTTTATTATCCGTGTTATTAAAAAAAAAACGGGTTTCTTAATCAAAAACAAAACCACACGGCCCGTAATCAAGCGTCAGCCCcagcag                                                                                                                       <  1:2408854/149‑1 (MQ=255)

                                       aCGCTTTAATTTCCAAAACGCGACGGCTGTTTTATTATCAACGCTGTAACGCCCTTGATGATCCGATTTATCACAAATAAAACCGGGTTCGTAATAATCAATAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACAtgt                                                                                                               <  1:3824200/149‑1 (MQ=255)

                                          cTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACAtgttgt                                                                                                            >  2:3806481/1‑149 (MQ=255)

                                          cTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACAtgttgt                                                                                                            >  2:1212276/1‑149 (MQ=255)

                                            gTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCg                                                                                                          >  1:188122/1‑149 (MQ=255)

                                                aTTCCACCCCGCGCCGGCTGTTTGTTTATCAACGCTGTACCGCCGTTGATGATCCGTGTGATAACAAATAAACCGGGGTGCGTAATCATCAAGAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTAt                                                                                                      <  1:1516592/149‑1 (MQ=255)

                                                   ccccaccgcgCCGGCTGTTTGATTAACAACGCTTTAACGCCCTTGTTGATCCTAGTTATAAAAAATAAACCCGGGTTCTTAAACAAAAACAAAACCCCACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATtca                                                                                                   <  1:664188/143‑1 (MQ=255)

                                                   ccACACCGCGACGGATGGTTTATTAAAAAAGCTGTAACGCCCTTTATGATCCTAGTGATTAAAAATAAAACCGGGTTCTTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATtca                                                                                                   <  2:30162/149‑1 (MQ=255)

                                                     acaACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATtcatc                                                                                                 >  1:1670854/1‑149 (MQ=255)

                                                       aaCGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAc                                                                                               >  2:4174265/1‑149 (MQ=255)

                                                        aCGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAcc                                                                                              >  2:292525/1‑149 (MQ=255)

                                                          gcgACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAcccc                                                                                            >  1:4120621/1‑149 (MQ=255)

                                                             aCGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATg                                                                                         >  2:1115263/1‑149 (MQ=255)

                                                              cGGCTGGTTTTTTATCAAGGGTGAACCGCCCTTGTTGCTCCGTGTTATAAAAAATAACACCGGGTTCGTAACAATCAATAAACCCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGa                                                                                        <  1:569938/149‑1 (MQ=255)

                                                              cGGCTGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGa                                                                                        <  2:3786851/149‑1 (MQ=255)

                                                                gCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGc                                                                                      >  2:3369452/1‑149 (MQ=255)

                                                                   ggTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCaaa                                                                                   >  2:2083287/1‑149 (MQ=255)

                                                                        aTTATCAAAGTTGTAAGGCCGTTGATGATCCGAGTGTTTAAAAATAAAACGGGTTTGGTAATCATCAATAAAACAAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGa                                                                              <  2:2308357/149‑1 (MQ=255)

                                                                         ttATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGAc                                                                             <  2:3859001/149‑1 (MQ=255)

                                                                          tATCAAAGCTGTAACGCCCTTGATGAACCGTGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGAcc                                                                            <  2:3532582/149‑1 (MQ=255)

                                                                           aTCAAAGCTGTAACGCCCTTTTTGATCCGTGTTATTAAAAATAAAACCGGGTTCTAAATAATAAAGAAAACCACCCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCg                                                                           <  2:3049992/149‑1 (MQ=255)

                                                                            tCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGt                                                                          >  1:4525234/1‑149 (MQ=255)

                                                                             cAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTc                                                                         >  2:31719/1‑149 (MQ=255)

                                                                               aaGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTg                                                                       >  1:3370177/1‑149 (MQ=255)

                                                                                  cTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCa                                                                    >  1:3081153/1‑149 (MQ=255)

                                                                                   tGTAACGCCCTTGATGATCCGAGTGATTACAAATAAACCCGGGTTCGTAATCATCAAGAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCAt                                                                   <  2:1260675/149‑1 (MQ=255)

                                                                                      aaCGCCCTTGATGATCCGAGTTATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTgg                                                                <  1:435491/149‑1 (MQ=255)

                                                                                        cGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGc                                                              <  2:2043033/149‑1 (MQ=255)

                                                                                        cGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGc                                                              >  1:213139/1‑149 (MQ=255)

                                                                                          cccTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCaa                                                            >  2:1147918/1‑149 (MQ=255)

                                                                                           ccTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAt                                                           >  1:2849882/1‑149 (MQ=255)

                                                                                           ccTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAt                                                           >  1:877931/1‑149 (MQ=255)

                                                                                           ccTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAt                                                           >  2:1838648/1‑149 (MQ=255)

                                                                                            cTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCACtt                                                          >  2:4421896/1‑149 (MQ=255)

                                                                                              tgatgaTCCGAGTGATTACAAATAAAACCGGGTTCTTAATCATCAAGAAAACAAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTaa                                                        <  2:1993305/149‑1 (MQ=255)

                                                                                                           gATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGt                                           >  1:1052289/1‑149 (MQ=255)

                                                                                                           gATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGt                                           >  2:4966290/1‑149 (MQ=255)

                                                                                                            aTTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTg                                          >  2:276925/1‑149 (MQ=255)

                                                                                                            aTTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTg                                          >  1:2575385/1‑149 (MQ=255)

                                                                                                            aTTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTg                                          >  2:1780733/1‑149 (MQ=255)

                                                                                                             ttAAAAATAAAACGGGGTTCTTAATAATAAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGc                                         <  2:3953248/149‑1 (MQ=255)

                                                                                                                cAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGac                                      >  2:610864/1‑149 (MQ=255)

                                                                                                                 aaaTAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGaca                                     >  2:2550931/1‑149 (MQ=255)

                                                                                                                  aaTAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGacaa                                    >  1:150593/1‑149 (MQ=255)

                                                                                                                   aTAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGacaac                                   >  1:3064308/1‑149 (MQ=255)

                                                                                                                    tAAAACGGGGTTCGAAATCATCAAGAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGacaaca                                  <  2:1879900/149‑1 (MQ=255)

                                                                                                                      aaaCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATc                                >  2:3710044/1‑149 (MQ=255)

                                                                                                                      aaaCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGAAATCAAGCGTCAGCCACAGCAGCGACATGTTGTCGGTATTCATCACCCCAAGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGGACGTGCGACAACATc                                >  1:4830457/1‑149 (MQ=255)

                                                                                                                       aaCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCg                               >  2:4329240/1‑149 (MQ=255)

                                                                                                                           gggTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTaa                           >  2:4255100/1‑149 (MQ=255)

                                                                                                                            tgTTCTTAATCATCAAGAAAACAAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTaaa                          <  2:1333527/148‑1 (MQ=255)

                                                                                                                            ggTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTaaa                          <  2:2280310/149‑1 (MQ=255)

                                                                                                                            ggTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTaaa                          >  1:4460437/1‑149 (MQ=255)

                                                                                                                             tttCGTAATCATCAATAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAc                         <  1:2373594/148‑1 (MQ=255)

                                                                                                                             gTTCGTAATCATCAAGAAACCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAc                         <  1:302614/149‑1 (MQ=255)

                                                                                                                             gTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAc                         >  2:4546079/1‑149 (MQ=255)

                                                                                                                             gTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAc                         >  1:2260011/1‑149 (MQ=255)

                                                                                                                                 gTAATCATCAATAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCaga                     <  2:2424485/149‑1 (MQ=255)

                                                                                                                                   aaTCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAgaga                   <  2:4143179/149‑1 (MQ=255)

                                                                                                                                    atcatcAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGAc                  >  1:4207022/1‑149 (MQ=255)

                                                                                                                                        tcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTa              <  2:4892562/149‑1 (MQ=255)

                                                                                                                                        tcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTa              >  2:1971032/1‑149 (MQ=255)

                                                                                                                                         caAGAAACCCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAt             <  1:4884521/149‑1 (MQ=255)

                                                                                                                                         caAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAt             <  2:1675204/149‑1 (MQ=255)

                                                                                                                                          ataaaacCCAACCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAtt            <  1:1884004/146‑1 (MQ=255)

                                                                                                                                          aacaaaaCCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAtt            <  1:3559865/146‑1 (MQ=255)

                                                                                                                                          aaGAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCTACATGTTGTCGGTATACATCACCCCATTAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAtt            >  1:2533533/1‑149 (MQ=255)

                                                                                                                                           ataaaaCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAttt           <  2:4740259/147‑1 (MQ=255)

                                                                                                                                           aGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTAttt           >  1:4135303/1‑149 (MQ=255)

                                                                                                                                            gAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTg          >  2:1540203/1‑149 (MQ=255)

                                                                                                                                            gAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTg          >  1:2544109/1‑149 (MQ=255)

                                                                                                                                             aaaaCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGt         >  1:3584435/1‑149 (MQ=255)

                                                                                                                                              aaaCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTc        >  2:1649786/1‑149 (MQ=255)

                                                                                                                                               aaCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTcc       <  2:923449/149‑1 (MQ=255)

                                                                                                                                                aCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCt      >  2:4768586/1‑149 (MQ=255)

                                                                                                                                                 ccAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtc     >  1:515851/1‑149 (MQ=255)

                                                                                                                                                 ccAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGACGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGAGACAACATCGCTAAACCAGATACGGTATTTGTCCtc     >  1:622974/1‑149 (MQ=255)

                                                                                                                                                  cAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACCACATCGCTAAACCAGAGACGGTATTTGTCCtca    >  2:2937165/1‑149 (MQ=255)

                                                                                                                                                  cAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtca    <  2:846608/149‑1 (MQ=255)

                                                                                                                                                  aaaaCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtca    <  1:775972/148‑1 (MQ=255)

                                                                                                                                                    aaCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc  >  1:3715816/1‑149 (MQ=255)

                                                                                                                                                    aaCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc  >  1:2731834/1‑149 (MQ=255)

GGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATC  >  CP009273/1784575‑1784871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`