BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,850,966	G→A	8.5%	L74F (CTC→TTC)	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,850,966	0	G	A	8.5%	1001.0 / 58.1	294	L74F (CTC→TTC)	ydjH	putative kinase
Reads supporting (aligned to +/- strand): ref base G (138/130); new base A (15/10); total (153/141)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 301 total aligned reads displayed.

ATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATA  >  CP009273/1850818‑1851114

  atTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGaa                                                                                                                                                    >  2:534764/1‑149 (MQ=255)

   ttCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAAt                                                                                                                                                   <  2:3922637/149‑1 (MQ=255)

    tCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATa                                                                                                                                                  >  1:4322744/1‑149 (MQ=255)

      aaTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATaaa                                                                                                                                                >  2:2071914/1‑149 (MQ=255)

      aaTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATaaa                                                                                                                                                <  1:3990869/149‑1 (MQ=255)

        tttCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAAtt                                                                                                                                              <  1:3283264/149‑1 (MQ=255)

           ccACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGcc                                                                                                                                           <  2:2006769/149‑1 (MQ=255)

               aGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGc                                                                                                                                       >  1:1056862/1‑149 (MQ=255)

                gACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                                                                                                                      >  2:18005/1‑149 (MQ=255)

                gACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAAAATAAATTGCCCTGCg                                                                                                                                      >  1:3901608/1‑149 (MQ=255)

                  cTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGc                                                                                                                                    >  2:3973413/1‑149 (MQ=255)

                    gCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCAt                                                                                                                                  >  2:4872953/1‑149 (MQ=255)

                    gCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCAt                                                                                                                                  >  2:3406966/1‑149 (MQ=255)

                     ccATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATc                                                                                                                                 >  1:4614317/1‑149 (MQ=255)

                       aTTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCtt                                                                                                                               <  2:1681866/149‑1 (MQ=255)

                          aCGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTAc                                                                                                                            >  1:334969/1‑149 (MQ=255)

                            ggTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTTACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCa                                                                                                                          >  2:3617337/1‑149 (MQ=255)

                            ggTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCa                                                                                                                          >  1:4804828/1‑149 (MQ=255)

                             gTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCaa                                                                                                                         >  1:4526927/1‑149 (MQ=255)

                                 gtgACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACg                                                                                                                     >  1:1165037/1‑149 (MQ=255)

                                  tgACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGa                                                                                                                    >  1:162260/1‑149 (MQ=255)

                                       aaCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAt                                                                                                               >  2:800515/1‑149 (MQ=255)

                                        aCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAtt                                                                                                              >  2:639585/1‑149 (MQ=255)

                                        aCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAtt                                                                                                              >  2:1927500/1‑149 (MQ=255)

                                        aCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAtt                                                                                                              <  2:215042/149‑1 (MQ=255)

                                        aCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAtt                                                                                                              >  1:2166554/1‑149 (MQ=255)

                                           tCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAAt                                                                                                           <  1:425999/149‑1 (MQ=255)

                                           tCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAAt                                                                                                           >  2:4476821/1‑149 (MQ=255)

                                              gCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCt                                                                                                        <  2:2455546/149‑1 (MQ=255)

                                                 cGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCtgtg                                                                                                     >  1:4600479/1‑149 (MQ=255)

                                                     aTCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGAt                                                                                                 <  1:3101130/149‑1 (MQ=255)

                                                      tcctccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATg                                                                                                <  1:1899679/149‑1 (MQ=255)

                                                      tcctccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATg                                                                                                <  1:531919/149‑1 (MQ=255)

                                                          ccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGccc                                                                                            <  1:1087403/149‑1 (MQ=255)

                                                             tCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGa                                                                                         <  2:4392579/149‑1 (MQ=255)

                                                              cACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGAc                                                                                        <  2:2261915/149‑1 (MQ=255)

                                                              cACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGAc                                                                                        <  1:2768263/149‑1 (MQ=255)

                                                                ccAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACga                                                                                      <  2:1508267/149‑1 (MQ=255)

                                                                ccAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACga                                                                                      <  2:2498085/149‑1 (MQ=255)

                                                                ccAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACga                                                                                      >  2:3179424/1‑149 (MQ=255)

                                                                ccAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACga                                                                                      >  1:4629779/1‑149 (MQ=255)

                                                                  aGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACgaga                                                                                    <  1:4845701/149‑1 (MQ=255)

                                                                  aGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACgaga                                                                                    >  1:824093/1‑149 (MQ=255)

                                                                    tCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGaaa                                                                                  <  1:5018967/149‑1 (MQ=255)

                                                                    tCCAAAGTTAATAGAGGTATCTATGCTGACGTCCTTCTTCAGGATTTTAATATCAATATTCTCTTTGAGGAAATTGTCTATAATAAATTTCCCTGATGCATCTTTACAAATACGACTCATTAATTATGTGCGATTGCCCAGACTAGaaa                                                                                  <  2:2796594/149‑1 (MQ=255)

                                                                     ccAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAaat                                                                                 >  1:3301362/1‑149 (MQ=255)

                                                                     ccAACGTTAAGAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAaat                                                                                 <  2:49706/149‑1 (MQ=255)

                                                                      cAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAaata                                                                                <  2:2552816/149‑1 (MQ=255)

                                                                      cAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAaata                                                                                >  1:111204/1‑149 (MQ=255)

                                                                           ttAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAAttgt                                                                           >  1:4678978/1‑149 (MQ=255)

                                                                           ttAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAAttgt                                                                           <  1:3809935/149‑1 (MQ=255)

                                                                           ttAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAAttgt                                                                           >  2:1853196/1‑149 (MQ=255)

                                                                           ttAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAAttgt                                                                           >  2:4581793/1‑149 (MQ=255)

                                                                              aTAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGc                                                                        <  1:4182731/149‑1 (MQ=255)

                                                                               tAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCt                                                                       <  2:935878/149‑1 (MQ=255)

                                                                                  agGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCa                                                                    <  1:1382002/149‑1 (MQ=255)

                                                                                   ggTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCAt                                                                   >  2:4226810/1‑149 (MQ=255)

                                                                                    gTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCAtt                                                                  <  2:3301362/149‑1 (MQ=255)

                                                                                    gTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCAtt                                                                  <  2:4142190/149‑1 (MQ=255)

                                                                                     tATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCAttt                                                                 >  1:2673816/1‑149 (MQ=255)

                                                                                      aTCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTa                                                                >  1:3701774/1‑149 (MQ=255)

                                                                                          aTGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGc                                                            <  1:2524613/149‑1 (MQ=255)

                                                                                             cTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATc                                                         <  2:4528212/149‑1 (MQ=255)

                                                                                             cTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATc                                                         >  1:1943621/1‑149 (MQ=255)

                                                                                             cTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATc                                                         <  2:3850536/149‑1 (MQ=255)

                                                                                              tGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCa                                                        <  1:3655866/149‑1 (MQ=255)

                                                                                               gACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCAc                                                       <  1:1504952/149‑1 (MQ=255)

                                                                                                   tCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCAccccc                                                   >  2:3556378/1‑149 (MQ=255)

                                                                                                     cTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCgg                                                 >  1:4102530/1‑149 (MQ=255)

                                                                                                      tGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCggt                                                <  1:3560275/149‑1 (MQ=255)

                                                                                                      tGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCggt                                                <  1:2280627/149‑1 (MQ=255)

                                                                                                       gCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCggtg                                               >  1:1000442/1‑149 (MQ=255)

                                                                                                        cTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCggtgg                                              >  2:5082153/1‑149 (MQ=255)

                                                                                                        cTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCggtgg                                              >  1:5086572/1‑149 (MQ=255)

                                                                                                        cTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTGTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCggtgg                                              <  2:4624686/149‑1 (MQ=255)

                                                                                                           cAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCa                                           >  2:3096135/1‑149 (MQ=255)

                                                                                                           cAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCa                                           <  1:407706/149‑1 (MQ=255)

                                                                                                            aGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCAt                                          <  2:2996679/149‑1 (MQ=255)

                                                                                                                   gAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGAtt                                   <  2:731744/149‑1 (MQ=255)

                                                                                                                      tatCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCtt                                <  2:334969/149‑1 (MQ=255)

                                                                                                                      tatCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCtt                                >  1:1295898/1‑149 (MQ=255)

                                                                                                                       atCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCttt                               <  2:1093446/149‑1 (MQ=255)

                                                                                                                        tCAATATTCTCTTTGCGGCAATGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTc                              <  1:2668675/149‑1 (MQ=255)

                                                                                                                             atTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAagag                         <  2:2867001/149‑1 (MQ=255)

                                                                                                                                ctctTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGAt                      <  1:5064004/149‑1 (MQ=255)

                                                                                                                                 tctTTGCGGCAATGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATa                     <  2:2334693/149‑1 (MQ=255)

                                                                                                                                 tctTTGCGGCAATGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATa                     <  2:3378859/149‑1 (MQ=255)

                                                                                                                                  ctTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATaa                    <  1:2012461/149‑1 (MQ=255)

                                                                                                                                     tGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGaa                 <  1:1599697/149‑1 (MQ=255)

                                                                                                                                        ggCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATcc              >  2:4548359/1‑149 (MQ=255)

                                                                                                                                        ggCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATcc              >  2:1955213/1‑149 (MQ=255)

                                                                                                                                        ggCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATcc              <  2:3874308/149‑1 (MQ=255)

                                                                                                                                          cAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCcac            <  2:4805465/149‑1 (MQ=255)

                                                                                                                                           aaTGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCcaca           >  1:30197/1‑149 (MQ=255)

                                                                                                                                           aaTGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCcaca           >  1:1788370/1‑149 (MQ=255)

                                                                                                                                            aTGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACAt          >  2:1303591/1‑149 (MQ=255)

                                                                                                                                            aTGGTCGAAAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACAt          >  2:1214112/1‑149 (MQ=255)

                                                                                                                                                  gagaATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGa    >  1:2708266/1‑149 (MQ=255)

                                                                                                                                                  gagaATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGa    >  1:4365007/1‑149 (MQ=255)

                                                                                                                                                    gaATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGata  >  1:2979833/1‑149 (MQ=255)

ATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGACGAGAAATAATTGTTGCTTCATTTATTGCATCACCCCCGGTGGTCATTGCGATTCTTTCAAGAGGATAAGAATCCACATCAAAGATA  >  CP009273/1850818‑1851114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`