BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,984,340	G→A	11.5%	L393L (TTG→TTA)	tyrP →	tyrosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,984,340	0	G	A	11.5%	916.0 / 82.1	287	L393L (TTG→TTA)	tyrP	tyrosine transporter
Reads supporting (aligned to +/- strand): ref base G (115/139); new base A (17/16); total (132/155)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 289 total aligned reads displayed.

GGCGGTACTGGCATTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATC  >  CP009273/1984193‑1984488

ggCGGTACTGGCATTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGa                                                                                                                                                     >  1:1175835/1‑149 (MQ=255)

 gCGGTACTGGCATTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGAt                                                                                                                                                    >  2:3945460/1‑149 (MQ=255)

  cGGTACTGGCATTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGAtt                                                                                                                                                   <  1:858108/149‑1 (MQ=255)

    gTACTGGCATTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGc                                                                                                                                                 >  2:2774591/1‑149 (MQ=255)

          gCATTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAgg                                                                                                                                           <  1:3639358/149‑1 (MQ=255)

          gCATTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTAATTGCGGCAgg                                                                                                                                           <  1:18415/149‑1 (MQ=255)

            aTTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgt                                                                                                                                         <  2:2720392/149‑1 (MQ=255)

            aTTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgt                                                                                                                                         <  1:4313526/149‑1 (MQ=255)

            aTTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgt                                                                                                                                         <  2:4459340/149‑1 (MQ=255)

             ttGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgtt                                                                                                                                        >  1:2421561/1‑149 (MQ=255)

               gATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGCGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttgt                                                                                                                                      <  1:2162961/149‑1 (MQ=255)

               gATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCGGGTGGTGGTGTTTCTCTGTGGTAGTGCTGTGATTGGCGTGCAATTTTGGAGTGCGGCAGGgttgt                                                                                                                                      <  2:508323/149‑1 (MQ=255)

                   aTCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTAcc                                                                                                                                  >  2:4669668/1‑149 (MQ=255)

                    tCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCa                                                                                                                                 >  2:4595994/1‑149 (MQ=255)

                    tCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCa                                                                                                                                 >  2:4520677/1‑149 (MQ=255)

                    tCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCa                                                                                                                                 >  1:763828/1‑149 (MQ=255)

                    tCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCa                                                                                                                                 >  1:4000617/1‑149 (MQ=255)

                    tCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCa                                                                                                                                 <  2:861309/149‑1 (MQ=255)

                        ttCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAg                                                                                                                             >  1:4703722/1‑149 (MQ=255)

                         tCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGt                                                                                                                            <  1:4600497/149‑1 (MQ=255)

                          cGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTg                                                                                                                           <  1:4101822/149‑1 (MQ=255)

                                tGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGa                                                                                                                     <  2:2328336/149‑1 (MQ=255)

                                 gACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGAt                                                                                                                    <  1:4781099/149‑1 (MQ=255)

                                 gACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGAt                                                                                                                    <  1:1314497/149‑1 (MQ=255)

                                 gACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGAt                                                                                                                    >  1:2930986/1‑149 (MQ=255)

                                  aCCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATc                                                                                                                   <  1:2566400/149‑1 (MQ=255)

                                  aCCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATc                                                                                                                   >  2:3748463/1‑149 (MQ=255)

                                   ccTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCa                                                                                                                  >  2:778576/1‑149 (MQ=255)

                                     tGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGa                                                                                                                >  1:3024067/1‑149 (MQ=255)

                                       gCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTAATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATa                                                                                                              >  1:3597891/1‑149 (MQ=255)

                                        cAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAg                                                                                                             <  1:59121/149‑1 (MQ=255)

                                         aaaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGc                                                                                                            >  1:2636451/1‑149 (MQ=255)

                                          aaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGcc                                                                                                           <  1:3176247/149‑1 (MQ=255)

                                          aaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGcc                                                                                                           >  2:5171742/1‑149 (MQ=255)

                                          aaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGcc                                                                                                           <  1:288200/149‑1 (MQ=255)

                                          aaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTAATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGcc                                                                                                           >  1:2838684/1‑149 (MQ=255)

                                           aGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCt                                                                                                          >  1:5097869/1‑149 (MQ=255)

                                              aGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCaa                                                                                                       >  2:186086/1‑149 (MQ=255)

                                              aGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCaa                                                                                                       >  1:307076/1‑149 (MQ=255)

                                               gAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCaaa                                                                                                      <  2:2043377/149‑1 (MQ=255)

                                               gAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCaaa                                                                                                      <  2:1241162/149‑1 (MQ=255)

                                                 aaGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAAtt                                                                                                    <  1:2722752/149‑1 (MQ=255)

                                                     acaATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCtt                                                                                                <  2:3567149/149‑1 (MQ=255)

                                                       aaTCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTAATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTAt                                                                                              >  2:4799317/1‑149 (MQ=255)

                                                           cTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTggg                                                                                          >  2:3340968/1‑149 (MQ=255)

                                                             cAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTg                                                                                        >  1:381921/1‑149 (MQ=255)

                                                             cAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTg                                                                                        >  1:1892722/1‑149 (MQ=255)

                                                                 cGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAg                                                                                    <  1:3496322/149‑1 (MQ=255)

                                                                     ttACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAAtt                                                                                <  2:4977663/149‑1 (MQ=255)

                                                                        ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                                                             <  2:2012048/149‑1 (MQ=255)

                                                                        ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                                                             <  2:3752809/149‑1 (MQ=255)

                                                                        ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                                                             <  2:1539771/149‑1 (MQ=255)

                                                                        ccGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTAATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                                                             >  2:2602660/1‑149 (MQ=255)

                                                                          gggTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGc                                                                           >  2:40707/1‑149 (MQ=255)

                                                                          gggTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGc                                                                           <  2:419143/149‑1 (MQ=255)

                                                                          gggTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGc                                                                           <  1:1484753/149‑1 (MQ=255)

                                                                             tCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGa                                                                        <  2:1390278/149‑1 (MQ=255)

                                                                                aaGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGacac                                                                     >  1:991921/1‑149 (MQ=255)

                                                                                aaGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGacac                                                                     >  1:3337261/1‑149 (MQ=255)

                                                                                aaGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGacac                                                                     >  1:5018575/1‑149 (MQ=255)

                                                                                 aGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAcc                                                                    <  2:3460759/149‑1 (MQ=255)

                                                                                 aGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAcc                                                                    >  2:2172402/1‑149 (MQ=255)

                                                                                  ggtggtCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAccc                                                                   <  1:1693637/149‑1 (MQ=255)

                                                                                    tggtCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCaa                                                                 <  2:1872064/149‑1 (MQ=255)

                                                                                    tggtCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTAGTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCaa                                                                 <  2:4000953/149‑1 (MQ=255)

                                                                                      gtcgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAAtt                                                               >  2:2506071/1‑149 (MQ=255)

                                                                                      gtcgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAAtt                                                               <  2:4855258/149‑1 (MQ=255)

                                                                                          tcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGc                                                           >  1:1146356/1‑149 (MQ=255)

                                                                                           ccGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGcc                                                          <  1:1451919/149‑1 (MQ=255)

                                                                                             ggCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCctc                                                        >  1:4456798/1‑149 (MQ=255)

                                                                                               cgcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCtt                                                      <  2:1719313/149‑1 (MQ=255)

                                                                                                gcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTa                                                     >  2:1824263/1‑149 (MQ=255)

                                                                                                 cTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTaa                                                    <  2:3243293/149‑1 (MQ=255)

                                                                                                   ggtggtggtGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATg                                                  <  2:2549253/149‑1 (MQ=255)

                                                                                                      ggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag                                               <  1:4872378/149‑1 (MQ=255)

                                                                                                        tggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggc                                             <  2:3694708/149‑1 (MQ=255)

                                                                                                         ggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcaggca                                            >  2:458650/1‑149 (MQ=255)

                                                                                                             tttCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAc                                        <  1:2999581/149‑1 (MQ=255)

                                                                                                                 tctGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCt                                    >  2:967058/1‑149 (MQ=255)

                                                                                                                 tctGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCt                                    >  1:4435863/1‑149 (MQ=255)

                                                                                                                   tgtgGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTa                                  >  2:531089/1‑149 (MQ=255)

                                                                                                                   tgtgGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTa                                  >  1:4515858/1‑149 (MQ=255)

                                                                                                                       gTATTGCTGTGATTGGCGTGCAATTTTTAATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAtt                              <  1:485993/149‑1 (MQ=255)

                                                                                                                          ttGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCt                           <  1:2342993/149‑1 (MQ=255)

                                                                                                                           tGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCtt                          <  1:1207271/149‑1 (MQ=255)

                                                                                                                           tGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCtt                          >  2:663963/1‑149 (MQ=255)

                                                                                                                              tgtgATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTAcc                       >  1:1932989/1‑149 (MQ=255)

                                                                                                                               gtgATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCa                      <  1:1517064/149‑1 (MQ=255)

                                                                                                                                tgATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCAc                     <  2:287701/149‑1 (MQ=255)

                                                                                                                                tgATTGGCGTGCAATTTTTAATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCAc                     <  2:1471236/149‑1 (MQ=255)

                                                                                                                                  aTTGGCGTGCAATTTTTAATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTa                   >  2:3289521/1‑149 (MQ=255)

                                                                                                                                     ggCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCg                <  2:631851/149‑1 (MQ=255)

                                                                                                                                       cGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCa              <  2:130324/149‑1 (MQ=255)

                                                                                                                                         tGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACg            >  1:2252201/1‑149 (MQ=255)

                                                                                                                                          gCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACgg           >  1:5188685/1‑149 (MQ=255)

                                                                                                                                           cAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACggg          <  2:296203/149‑1 (MQ=255)

                                                                                                                                           cAATTTTTAATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACggg          <  1:4066713/149‑1 (MQ=255)

                                                                                                                                            aaTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGc         <  2:436156/149‑1 (MQ=255)

                                                                                                                                              tttttGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCaa       >  1:3923314/1‑149 (MQ=255)

                                                                                                                                                 ttGAGTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGAGAGCCTCAAAGTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGa    <  2:51323/149‑1 (MQ=255)

GGCGGTACTGGCATTGATTATCCCTTCGCTGTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATC  >  CP009273/1984193‑1984488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`