BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,237,098	G→T	33.8%	H12N (CAT→AAT)	folE ←	GTP cyclohydrolase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,237,098	0	G	T	33.8%	488.6 / 298.9	291	H12N (CAT→AAT)	folE	GTP cyclohydrolase I
Reads supporting (aligned to +/- strand): ref base G (87/105); new base T (47/52); total (134/157)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

ATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACA  >  CP009273/2236950‑2237245

 tCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGa                                                                                                                                                    >  2:1506478/1‑149 (MQ=255)

   aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAAc                                                                                                                                                  >  1:725490/1‑149 (MQ=255)

   aaaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAAc                                                                                                                                                  <  1:3223959/149‑1 (MQ=255)

    aaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAAcc                                                                                                                                                 >  2:2935606/1‑149 (MQ=255)

      cTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAg                                                                                                                                               <  1:2062323/149‑1 (MQ=255)

          catcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGcc                                                                                                                                           <  2:1552639/149‑1 (MQ=255)

           atcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCg                                                                                                                                          <  1:4967964/149‑1 (MQ=255)

           atcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCg                                                                                                                                          >  1:857260/1‑149 (MQ=255)

               gCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGcttc                                                                                                                                      <  2:531686/149‑1 (MQ=255)

               gCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCAGAGGACCAGCAAGAAGGCTTTTGCGCGGTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGcttc                                                                                                                                      <  2:4568047/149‑1 (MQ=255)

                 cAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGcttctt                                                                                                                                    <  2:4837782/149‑1 (MQ=255)

                  aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCttt                                                                                                                                   >  2:4420342/1‑149 (MQ=255)

                  aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCttt                                                                                                                                   <  1:2658523/149‑1 (MQ=255)

                    gTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAc                                                                                                                                 <  1:773287/149‑1 (MQ=255)

                    gTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTAc                                                                                                                                 <  2:4121193/149‑1 (MQ=255)

                     tCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACt                                                                                                                                <  1:2232796/149‑1 (MQ=255)

                       gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGa                                                                                                                              <  1:2107939/149‑1 (MQ=255)

                        agaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCACTAAGGCGTTTGCGCGGTTCGGTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCAGGAACCAGGGCCGCTTCTTTACGGAg                                                                                                                             >  2:1664733/1‑149 (MQ=255)

                           ttCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                          <  1:2729236/149‑1 (MQ=255)

                           ttCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACAGAGTGa                                                                                                                          >  1:567598/1‑149 (MQ=255)

                           ttCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCGTCTTTACTGAGTGa                                                                                                                          >  1:2382/1‑149 (MQ=255)

                           ttCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGa                                                                                                                          <  2:3404914/149‑1 (MQ=255)

                               gcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGc                                                                                                                      <  2:2317482/149‑1 (MQ=255)

                               gcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGc                                                                                                                      <  2:548643/149‑1 (MQ=255)

                               gcagcTGCATGAGTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATGTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGc                                                                                                                      <  2:4686208/149‑1 (MQ=255)

                                    tGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTa                                                                                                                 <  1:1274718/149‑1 (MQ=255)

                                    tGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTa                                                                                                                 <  2:1327424/149‑1 (MQ=255)

                                     gCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTAt                                                                                                                >  1:1825464/1‑149 (MQ=255)

                                      cATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCATTACTGAGTGATGGCATTTAtt                                                                                                               >  1:2784244/1‑148 (MQ=255)

                                        tGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAt                                                                                                             >  2:13381/1‑149 (MQ=255)

                                        tGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGAGGGCATTTATGAt                                                                                                             >  2:2616306/1‑149 (MQ=255)

                                          aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTAGTTACTGAGTGATGGGCTTTATGAttt                                                                                                           >  1:3076224/1‑149 (MQ=255)

                                          aTTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATTGCATTTATGAttt                                                                                                           >  1:3428859/1‑149 (MQ=255)

                                              cGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTcc                                                                                                       <  1:599490/149‑1 (MQ=255)

                                                 tCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGc                                                                                                    >  2:3088232/1‑149 (MQ=255)

                                                  cATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCa                                                                                                   <  2:1888689/149‑1 (MQ=255)

                                                   atatGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAg                                                                                                  <  1:4125809/149‑1 (MQ=255)

                                                   atatGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTATTTAATGAGTGATTGCATTTATTATTTATAcagcag                                                                                                  >  1:934853/1‑149 (MQ=255)

                                                    tatGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAgg                                                                                                 <  1:559704/149‑1 (MQ=255)

                                                        aCCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGtgtg                                                                                             <  1:2385757/149‑1 (MQ=255)

                                                          cAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGAc                                                                                           <  1:4170324/149‑1 (MQ=255)

                                                          cAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGAc                                                                                           <  1:4037146/149‑1 (MQ=255)

                                                           aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACg                                                                                          >  2:426541/1‑149 (MQ=255)

                                                              aaTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTAATGAGTGATTGCATTTATGATTTCTCCTGCAGGTGTGAAGCCt                                                                                       >  1:3724308/1‑149 (MQ=255)

                                                               aTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTAATTAGTGATTGCATTTATTATTTCTCCAGCAGGTGTGACGccac                                                                                      >  1:1710051/1‑147 (MQ=255)

                                                                tAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                                                                     >  2:3890547/1‑149 (MQ=255)

                                                                  aGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATTATTTCTCCTGCAGGTGTGACGCCTCCGc                                                                                   >  2:2048322/1‑149 (MQ=255)

                                                                  aGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTACTGCAGGTGTTACGCCTCCGc                                                                                   >  2:4920147/1‑149 (MQ=255)

                                                                     cTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTAATGAGTGATGGCATTTATGATTTATCCTGCAGGTGTGACGCCTCCGCCCt                                                                                >  2:5133965/1‑149 (MQ=255)

                                                                      ttttGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTAGTTAAATATTGTTTGCATTTATTATTTAAACATCAGGTGTGACGCCTccccccag                                                                               >  1:297072/1‑147 (MQ=255)

                                                                              gTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTATCCAGCAGGTGTGACGCCTCCGCCCAGCGgggggg                                                                       >  1:1042450/1‑149 (MQ=255)

                                                                              gTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTggggg                                                                       >  2:2471254/1‑149 (MQ=255)

                                                                               tttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTATTTACTGAGTGATTGCATTTATTATTTCTCCTGCAGGTGTGACGCCACCGCCCAGCGTGGGGGc                                                                      >  1:2007855/1‑149 (MQ=255)

                                                                               tttCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGc                                                                      >  2:4180277/1‑149 (MQ=255)

                                                                                   gTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAg                                                                  <  1:3783734/149‑1 (MQ=255)

                                                                                      aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                                                               <  2:3700211/149‑1 (MQ=255)

                                                                                      aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                                                               >  2:2249407/1‑149 (MQ=255)

                                                                                      aTCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                                                               >  2:2858153/1‑149 (MQ=255)

                                                                                        ccATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATTATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatt                                                             >  2:5044896/1‑149 (MQ=255)

                                                                                        ccATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCACGCGCAACTAACGCTTCATGAACCAGGGCCGCGTCTTTACTGAGAGATGGCAGTTATGATTTCTCCTGCAGGTGTGACGCCTCCCCCCGGCGGGGGGGCAAACttatt                                                             >  2:2028969/1‑149 (MQ=255)

                                                                                         cATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatta                                                            <  1:2291037/149‑1 (MQ=255)

                                                                                         cATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatta                                                            >  2:2145642/1‑149 (MQ=255)

                                                                                          aTTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATTATTTCTCCTGCAGGTGTGACGCCACCCCCCAGCGGGGGGGCAAAGTTATTaa                                                           >  1:2913592/1‑149 (MQ=255)

                                                                                             tCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCt                                                        <  2:429314/149‑1 (MQ=255)

                                                                                               aTGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCAGTTATTATTTATCCTGCAGGTGTGACGCCAACGCCCTGCGGGGGGGCAAAGTTATTAATCTGa                                                      >  1:4232563/1‑149 (MQ=255)

                                                                                               aTGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCGTGTTTAATGAGTGATGGGATGTATGTTTTCTCCGGCAGGTGGGTCGCCGCCGCCCTGCGGGGGGGGGCAGTTATTAAGCTgg                                                      >  1:4412471/1‑148 (MQ=255)

                                                                                                tGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAt                                                     <  2:567696/149‑1 (MQ=255)

                                                                                                tGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGAt                                                     >  2:670757/1‑149 (MQ=255)

                                                                                                    cgggcgggCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAc                                                 <  2:772269/149‑1 (MQ=255)

                                                                                                         gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCt                                            <  1:145201/149‑1 (MQ=255)

                                                                                                         gggcgCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTATTTACTGAGTGATGGCATTTATGATTTATCCAGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACATCCt                                            >  1:2557838/1‑149 (MQ=255)

                                                                                                           gcgcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAGTTATTAATCTGATTTACAGCCTGa                                          >  2:1143247/1‑149 (MQ=255)

                                                                                                             gcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatt                                        <  1:186316/149‑1 (MQ=255)

                                                                                                             gcAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGatt                                        <  1:5027564/149‑1 (MQ=255)

                                                                                                                gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCAAAGTTATTAAGCTGATTTTCAGCCTGattatt                                     >  1:396018/1‑149 (MQ=255)

                                                                                                                gCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTCTTTACGGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCAGCGTGGGGGCCAAGTTATTAAGCTGATTTACAGCCTGattatt                                     >  2:2703697/1‑149 (MQ=255)

                                                                                                                  ggTGTTTCCAGTCCTCGCGCAACTAACGCTTCATTAACCAGGGCCGCTTGTTTTATGAGTGTTTGCAGTTATGTTTTATACTGCAGGTGTGCCGCCACCGCCCAGCGGGGGGGCCAAGGTATTAAGCAGATTTACAGCCAGattatttt                                   >  1:473304/1‑147 (MQ=255)

                                                                                                                  ggTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATtgt                                   <  1:3069240/149‑1 (MQ=255)

                                                                                                                      tttCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGt                               <  1:5109449/149‑1 (MQ=255)

                                                                                                                      tttCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGt                               <  2:3793911/149‑1 (MQ=255)

                                                                                                                           aGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTAATGAGTGATGGCATTTATGATTTCTCCTGCAGGGGTGACGCCTCCGCCCTGCGTGGGGGCAAAGGTATTAATCTGATTTACAGCCTGATTATTTTGCGTGAggc                          >  2:3131649/1‑149 (MQ=255)

                                                                                                                            gTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGGTGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcg                         >  2:1237858/1‑149 (MQ=255)

                                                                                                                            gTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcg                         <  1:3330312/149‑1 (MQ=255)

                                                                                                                            gTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAggcg                         <  1:3354963/149‑1 (MQ=255)

                                                                                                                                   cgcAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGcaca                  <  2:1964701/149‑1 (MQ=255)

                                                                                                                                    gcAACTAACGCTTCATGAACCAGGGCCGCGTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACAt                 <  2:4602352/149‑1 (MQ=255)

                                                                                                                                     cAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATa                <  1:1056585/149‑1 (MQ=255)

                                                                                                                                      aaCTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCCAAGTTATTAATCTGATTTACAGCCTGATTATTTTGCGTGTTGCGGCGCACATaa               >  1:3015115/1‑149 (MQ=255)

                                                                                                                                      aaCTAACGCTTCATGAACCAGGGCCGCGTCTTTACTGAGTGATGGCCTTTATGATTTCTCCAGCAGGGGTGACGCCGCCCCCCAGCGGGGGGGCCCACTTATTAAGCGGCTTTACAGCCAGATTATTGTGCGTGAGGCGGCGcaccaaa               >  1:5084295/1‑145 (MQ=255)

                                                                                                                                         tAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATcc            <  1:4650720/149‑1 (MQ=255)

                                                                                                                                          aaCGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCa           <  2:4132729/149‑1 (MQ=255)

                                                                                                                                           aCGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAg          <  1:814578/149‑1 (MQ=255)

                                                                                                                                           aCGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAg          <  2:3647794/149‑1 (MQ=255)

                                                                                                                                            cGCTTCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGt         <  2:1595276/149‑1 (MQ=255)

                                                                                                                                               ttCATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTAtt      <  1:2196294/149‑1 (MQ=255)

                                                                                                                                                 cATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTca    <  1:4566669/149‑1 (MQ=255)

                                                                                                                                                 cATTAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTca    <  1:1557056/149‑1 (MQ=255)

                                                                                                                                                   tGAACCAGGGCCGCTTCTTTACTGAGTGATTGCATTTATGATTTCTCCAGCACGTGTGACGCCACCCCCCAGCGTGGGGGCAAAGTTATTAAGCAGAATTACAGCCAGATTATATTGCGTGAGGCGGCGCACCAAATCCACAATTcaca  >  1:5156399/1‑149 (MQ=255)

                                                                                                                                                   tGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTcaca  <  1:487277/149‑1 (MQ=255)

                                                                                                                                                   tGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTcaca  <  2:1179084/149‑1 (MQ=255)

ATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACA  >  CP009273/2236950‑2237245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`