BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	214,044	G→T	7.6%	R407S (CGT→AGT)	proS ←	prolyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	214,044	0	G	T	7.6%	1304.5 / 61.5	340	R407S (CGT→AGT)	proS	prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (146/168); new base T (13/13); total (159/181)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 342 total aligned reads displayed.

ATCGCCGCAGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATC  >  CP009273/213896‑214191

 tCGCCGCAGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGt                                                                                                                                                    >  2:2977241/1‑149 (MQ=255)

 tCGCCGCAGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGt                                                                                                                                                    <  1:4947983/149‑1 (MQ=255)

  cgccgcAGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGtt                                                                                                                                                   >  2:3306808/1‑149 (MQ=255)

    ccgcAGCTACCACACGCGTTAACCCGATACCGTAGCAACCCATCGTCATGATTTGGTTACGTACATCTTCACCCTGTACGGATGATTTCAGTGCTTCGGAGTACTTGTTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTg                                                                                                                                                 <  1:3808509/149‑1 (MQ=255)

      gcAGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGAt                                                                                                                                               >  1:4260434/1‑149 (MQ=255)

      gcAGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGAt                                                                                                                                               <  2:3985631/149‑1 (MQ=255)

       cAGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATc                                                                                                                                              <  2:4448253/149‑1 (MQ=255)

        aGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATca                                                                                                                                             <  2:4700704/149‑1 (MQ=255)

         gCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATcag                                                                                                                                            <  1:5129973/149‑1 (MQ=255)

           tACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATcagca                                                                                                                                          >  2:4470415/1‑149 (MQ=255)

           tACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTAACCAACTTCGATACCACGTTTGATcagca                                                                                                                                          >  2:4808079/1‑149 (MQ=255)

              cacaCGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGcc                                                                                                                                       <  2:3742620/149‑1 (MQ=255)

              cacaCGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGcc                                                                                                                                       >  2:2220659/1‑149 (MQ=255)

               acacGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCt                                                                                                                                      <  2:2889918/149‑1 (MQ=255)

               acacGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCt                                                                                                                                      <  1:3993500/149‑1 (MQ=255)

                cacGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACTTTTGATCAGCAGCCTa                                                                                                                                     >  2:916018/1‑149 (MQ=255)

                   gcgTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTAccc                                                                                                                                  <  2:5167961/149‑1 (MQ=255)

                    cgTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACTTTTGATCAGCAGCCTACCCt                                                                                                                                 <  1:4421647/149‑1 (MQ=255)

                    cgTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCt                                                                                                                                 <  1:2383765/149‑1 (MQ=255)

                    cgTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCt                                                                                                                                 <  2:391783/149‑1 (MQ=255)

                    cgTTACCCCGAGACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCt                                                                                                                                 <  1:4907754/149‑1 (MQ=255)

                     gTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTg                                                                                                                                <  2:5115356/149‑1 (MQ=255)

                        aCCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGcc                                                                                                                             <  2:875314/149‑1 (MQ=255)

                              aTACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCgg                                                                                                                       <  1:193232/149‑1 (MQ=255)

                                aCCGTAGCAACCCATCGTCAGGATTTGGGTACGGCCATCTTCACCCGGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCGGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGc                                                                                                                     <  2:1221661/149‑1 (MQ=255)

                                 ccGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCt                                                                                                                    >  2:5051869/1‑149 (MQ=255)

                                 ccGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCt                                                                                                                    <  2:2804309/149‑1 (MQ=255)

                                   gTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTg                                                                                                                  <  2:1942536/149‑1 (MQ=255)

                                    tAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTgg                                                                                                                 >  2:4610430/1‑149 (MQ=255)

                                    tAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTgg                                                                                                                 >  2:2017448/1‑149 (MQ=255)

                                     aGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGa                                                                                                                >  2:2160215/1‑149 (MQ=255)

                                      gCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACTTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGAt                                                                                                               <  2:349872/149‑1 (MQ=255)

                                      gCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGAt                                                                                                               >  2:1279508/1‑149 (MQ=255)

                                              tCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCgccagcc                                                                                                       >  2:2474620/1‑149 (MQ=255)

                                              tCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCgccagcc                                                                                                       >  1:2045129/1‑149 (MQ=255)

                                                  cAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGccacca                                                                                                   <  2:3105752/149‑1 (MQ=255)

                                                      aTTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGtt                                                                                               >  1:2581691/1‑149 (MQ=255)

                                                        ttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTAc                                                                                             >  2:4259053/1‑149 (MQ=255)

                                                        ttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTAc                                                                                             <  1:2933411/149‑1 (MQ=255)

                                                        ttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTAc                                                                                             <  1:5168233/149‑1 (MQ=255)

                                                            ttACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGat                                                                                         >  2:596545/1‑149 (MQ=255)

                                                              aCGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGatat                                                                                       <  1:2724067/149‑1 (MQ=255)

                                                                ggCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCt                                                                                     <  2:1125576/149‑1 (MQ=255)

                                                                  ccATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGc                                                                                   >  1:699966/1‑149 (MQ=255)

                                                                    aTCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCaa                                                                                 <  1:4563754/149‑1 (MQ=255)

                                                                     tCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAAc                                                                                >  2:4224272/1‑149 (MQ=255)

                                                                     tCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAAc                                                                                >  1:569232/1‑149 (MQ=255)

                                                                           cccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCgg                                                                          <  1:2095354/149‑1 (MQ=255)

                                                                              tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                                                                       <  2:2652510/149‑1 (MQ=255)

                                                                               gTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTa                                                                      <  1:4119480/149‑1 (MQ=255)

                                                                               gTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTa                                                                      >  1:3286341/1‑149 (MQ=255)

                                                                                 aCGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCAGCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                    <  2:1768798/149‑1 (MQ=255)

                                                                                   ggAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGa                                                                  <  1:4214920/149‑1 (MQ=255)

                                                                                   ggAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGa                                                                  >  1:80825/1‑149 (MQ=255)

                                                                                         tttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                                                            <  2:1360023/149‑1 (MQ=255)

                                                                                         tttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                                                            <  1:2220659/149‑1 (MQ=255)

                                                                                          ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACTTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                                                           <  1:2508200/149‑1 (MQ=255)

                                                                                          ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                                                           >  1:137781/1‑149 (MQ=255)

                                                                                            cAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGAt                                                         <  2:2406085/149‑1 (MQ=255)

                                                                                                gCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCa                                                     <  2:3134285/149‑1 (MQ=255)

                                                                                                  ttCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt                                                   <  1:3667964/149‑1 (MQ=255)

                                                                                                   tCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGtt                                                  <  2:1652512/149‑1 (MQ=255)

                                                                                                    cGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTg                                                 >  1:96101/1‑149 (MQ=255)

                                                                                                     ggAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                >  1:4671984/1‑149 (MQ=255)

                                                                                                     ggAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                <  1:164789/149‑1 (MQ=255)

                                                                                                      gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                               >  1:1601202/1‑149 (MQ=255)

                                                                                                      gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                               >  1:3637867/1‑149 (MQ=255)

                                                                                                       aGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATg                                              <  1:1035787/149‑1 (MQ=255)

                                                                                                        gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACTTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                             >  1:2403512/1‑149 (MQ=255)

                                                                                                        gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                             <  1:2306536/149‑1 (MQ=255)

                                                                                                            ttGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                         <  1:1796581/149‑1 (MQ=255)

                                                                                                              ggTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagt                                       <  2:3359093/149‑1 (MQ=255)

                                                                                                                  cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                   >  1:3168991/1‑149 (MQ=255)

                                                                                                                   ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtgt                                  <  1:1104954/149‑1 (MQ=255)

                                                                                                                     aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGttt                                <  2:3051753/149‑1 (MQ=255)

                                                                                                                      gCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTa                               >  2:2303131/1‑149 (MQ=255)

                                                                                                                       cTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTAc                              <  1:2324780/149‑1 (MQ=255)

                                                                                                                        tGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTAcc                             <  1:5051869/149‑1 (MQ=255)

                                                                                                                         ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                            >  1:1203052/1‑149 (MQ=255)

                                                                                                                         ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                            >  1:3798919/1‑149 (MQ=255)

                                                                                                                           aaGATGTGACCAACTTCGAGACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                          <  1:3233169/149‑1 (MQ=255)

                                                                                                                             gATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                        >  2:488296/1‑149 (MQ=255)

                                                                                                                              aTGTGACCACCTGCGATACCACGTTGGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACGTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGAt                       <  1:4636194/149‑1 (MQ=255)

                                                                                                                              aTGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGAt                       <  1:586116/149‑1 (MQ=255)

                                                                                                                                 tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGtt                    <  1:3753860/149‑1 (MQ=255)

                                                                                                                                  gACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTa                   >  1:3702138/1‑149 (MQ=255)

                                                                                                                                   aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAg                  >  2:3175284/1‑149 (MQ=255)

                                                                                                                                   aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAg                  >  1:3081285/1‑149 (MQ=255)

                                                                                                                                    ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTCCGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGGTTACCATCGATGTTAGc                 >  1:4299549/1‑149 (MQ=255)

                                                                                                                                    ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGc                 <  1:4026419/149‑1 (MQ=255)

                                                                                                                                    ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGc                 <  1:1428803/149‑1 (MQ=255)

                                                                                                                                     cAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCa                >  1:3562611/1‑149 (MQ=255)

                                                                                                                                     cAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCa                >  1:334676/1‑149 (MQ=255)

                                                                                                                                      aaCTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAc               <  1:1485894/149‑1 (MQ=255)

                                                                                                                                        cTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTTGTGTTTACCATCGATGTTAGCACca             <  2:1616588/149‑1 (MQ=255)

                                                                                                                                         ttCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcag            >  1:773628/1‑149 (MQ=255)

                                                                                                                                         ttCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcag            >  1:900898/1‑149 (MQ=255)

                                                                                                                                         ttCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcag            >  2:4127926/1‑149 (MQ=255)

                                                                                                                                             aTACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCagcagc        <  1:3452952/149‑1 (MQ=255)

                                                                                                                                                   cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATc  <  2:4975749/149‑1 (MQ=255)

ATCGCCGCAGCTACCACACGCGTTACCCCGATACCGTAGCAACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATC  >  CP009273/213896‑214191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`